SG10201809900PA - Hybrid Nanopore Sensors - Google Patents

Hybrid Nanopore Sensors

Info

Publication number
SG10201809900PA
SG10201809900PA SG10201809900PA SG10201809900PA SG10201809900PA SG 10201809900P A SG10201809900P A SG 10201809900PA SG 10201809900P A SG10201809900P A SG 10201809900PA SG 10201809900P A SG10201809900P A SG 10201809900PA SG 10201809900P A SG10201809900P A SG 10201809900PA
Authority
SG
Singapore
Prior art keywords
nanopores
nanopore sensors
solid
hybrid nanopore
detection apparatus
Prior art date
Application number
SG10201809900PA
Inventor
Boyan Boyanov
Jeffrey G Mandell
Kevin L Gunderson
Jingwei Bai
Liangliang Qiang
Bradley Baas
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of SG10201809900PA publication Critical patent/SG10201809900PA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Electrochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)

Abstract

HYBRID NANOPORE SENSORS In one aspect, a detection apparatus comprises a solid support with an array of solid- state nanopores and a plurality of lipid nanodiscs with protein nanopores, wherein the lipid nanodiscs are disposed on the solid support such that they form seals at the solid-state 5 nanopores. In another aspect, a detection apparatus comprises one or more nanopores that are surrounded by a membrane, wherein each nanopore is tethered to an electrode. Figure 2A
SG10201809900PA 2014-07-31 2015-07-29 Hybrid Nanopore Sensors SG10201809900PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462031762P 2014-07-31 2014-07-31
US201562157749P 2015-05-06 2015-05-06

Publications (1)

Publication Number Publication Date
SG10201809900PA true SG10201809900PA (en) 2018-12-28

Family

ID=53794530

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201700745XA SG11201700745XA (en) 2014-07-31 2015-07-29 Hybrid nanopore sensors
SG10201809900PA SG10201809900PA (en) 2014-07-31 2015-07-29 Hybrid Nanopore Sensors

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201700745XA SG11201700745XA (en) 2014-07-31 2015-07-29 Hybrid nanopore sensors

Country Status (10)

Country Link
US (4) US10519499B2 (en)
EP (4) EP3175237B1 (en)
JP (4) JP6601852B2 (en)
CN (3) CN113759105A (en)
AU (3) AU2015296551B2 (en)
CA (2) CA3163156A1 (en)
DK (1) DK3702780T3 (en)
ES (1) ES2912963T3 (en)
SG (2) SG11201700745XA (en)
WO (1) WO2016019030A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110914291A (en) * 2017-07-17 2020-03-24 哈佛大学校董委员会 Nanopore-matched protein shuttles for molecular characterization
CN108226249B (en) * 2018-01-09 2021-02-26 深圳市梅丽纳米孔科技有限公司 Disposable nanopore biosensor and manufacturing method thereof
KR102351743B1 (en) 2018-02-16 2022-01-14 일루미나, 인코포레이티드 device for sequencing
KR102127111B1 (en) * 2019-01-31 2020-06-26 경희대학교 산학협력단 Method for producing a nanopore and nanopore structure using the method
EP3953369A1 (en) * 2019-04-09 2022-02-16 Oxford Nanopore Technologies plc Pore
CN110292916B (en) * 2019-06-26 2020-11-03 中国科学院过程工程研究所 Phospholipid nanodisk chromatography medium and preparation method and application thereof
EP4003382A4 (en) * 2019-07-31 2023-10-11 Axbio Inc. Systems and methods for assessing a target molecule
CN110628599A (en) * 2019-10-19 2019-12-31 上海新微技术研发中心有限公司 Raman spectroscopy biomolecule sequencing system
JP7365022B2 (en) * 2019-10-28 2023-10-19 日本電信電話株式会社 Supports for biomolecules and their production methods
JP2023500627A (en) * 2019-10-30 2023-01-10 ストラトス ゲノミクス インコーポレイテッド Methods and compositions for assembly of biological nanopores
CN116622754A (en) * 2023-05-31 2023-08-22 南开大学 Fixed-point marked transmembrane protein nano-disk and preparation method and application thereof

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US7048949B2 (en) * 2000-11-20 2006-05-23 The Board Of Trustees Of The University Of Illinois Membrane scaffold proteins
US7083958B2 (en) * 2000-11-20 2006-08-01 The Board Of Trustees Of The University Of Illinois Membrane scaffold proteins
US7592008B2 (en) * 2000-11-20 2009-09-22 The Board Of Trustees Of The University Of Illinois, A Body Corporate And Politic Of The State Of Illinois Membrane scaffold proteins
US20050023156A1 (en) * 2003-07-30 2005-02-03 Ramsey J. Michael Nanostructured material transport devices and their fabrication by application of molecular coatings to nanoscale channels
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US20060231419A1 (en) * 2005-04-15 2006-10-19 Barth Philip W Molecular resonant tunneling sensor and methods of fabricating and using the same
US7638034B2 (en) * 2006-09-21 2009-12-29 Los Alamos National Security, Llc Electrochemical detection of single molecules using abiotic nanopores having electrically tunable dimensions
CN1932039B (en) * 2006-09-21 2010-06-16 上海交通大学 Single molecular nucleic acid sequencing process for exonuclease-nanometer hole
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
US20080254995A1 (en) * 2007-02-27 2008-10-16 Drexel University Nanopore arrays and sequencing devices and methods thereof
WO2008124706A2 (en) * 2007-04-06 2008-10-16 Arizona Board Of Regents Acting For And On Behalf Of Arizona State University Devices and methods for target molecule characterization
WO2008141230A1 (en) * 2007-05-09 2008-11-20 Lawrence Livermore National Security, Llc Methods and systems for monitoring production of a target protein in a nanolipoprotein particle
US8277628B2 (en) 2007-07-13 2012-10-02 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus using electric field for improved biological assays
GB0808856D0 (en) 2008-05-15 2008-06-25 Univ Warwick Fabricated nanopores and micropores for chemical and biochemical analysis
CN103695530B (en) 2008-07-07 2016-05-25 牛津纳米孔技术有限公司 Enzyme-hole construct
US20100099198A1 (en) * 2008-07-11 2010-04-22 Board Of Regents, The University Of Texas System Apparatus and system for pattern recognition sensing for biomolecules
EP3029467B1 (en) * 2008-09-22 2020-01-08 University of Washington Msp nanopores and related methods
CA2741373A1 (en) * 2008-10-30 2010-06-03 Peixuan Guo Membrane-integrated viral dna-packaging motor protein connector biosensor for dna sequencing and other uses
US8986928B2 (en) * 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
EP2534284B1 (en) * 2010-02-08 2021-03-17 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
CA3211104A1 (en) 2010-02-23 2011-09-01 University Of Washington Artificial mycolic acid membranes
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
EP2580588B1 (en) * 2010-06-08 2014-09-24 President and Fellows of Harvard College Nanopore device with graphene supported artificial lipid membrane
WO2012060882A2 (en) * 2010-11-02 2012-05-10 Molecular Sensing, Inc. Interferometric detection using nanoparticles
CN107083421A (en) * 2010-12-17 2017-08-22 纽约哥伦比亚大学理事会 The DNA detected using the nucleotides through modification and nano-pore is sequenced in synthesis
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
CN103380369B (en) * 2011-02-23 2016-12-28 纽约市哥伦比亚大学理事会 Nano-pore is used to carry out the system and method for Single Molecule Detection
US9702849B2 (en) * 2011-04-04 2017-07-11 President And Fellows Of Harvard College Nanopore sensing by local electrical potential measurement
EP3385992A1 (en) 2011-07-27 2018-10-10 The Board of Trustees of the University of Illinois Nanopore sensors for biomolecular characterization
US9758823B2 (en) 2011-10-21 2017-09-12 Oxford Nanopore Technologies Limited Enzyme method
EP3305400A3 (en) 2011-10-28 2018-06-06 Illumina, Inc. Microarray fabrication system and method
JP6312607B2 (en) * 2012-02-16 2018-04-18 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Nanopore sensors for enzyme-mediated protein translocation
CN108949944A (en) * 2012-02-16 2018-12-07 吉尼亚科技公司 Generate double-deck method for nanopore sensor
CN102621214B (en) * 2012-03-13 2014-10-29 美国哈佛大学 Method for carrying out deceleration and monomolecular capture on nucleic acid molecule based on solid-state nano hole
EP2834374A4 (en) 2012-04-04 2016-04-06 Univ Arizona Electrodes for sensing chemical composition
ES2608857T3 (en) * 2012-12-18 2017-04-17 Salipro Biotech Ag Salipro particles
EP3919624A3 (en) 2013-07-01 2021-12-29 Illumina, Inc. Catalyst-free surface functionalization and polymer grafting

Also Published As

Publication number Publication date
CN106796214B (en) 2020-01-14
ES2912963T3 (en) 2022-05-30
SG11201700745XA (en) 2017-02-27
WO2016019030A1 (en) 2016-02-04
CN110954686B (en) 2021-09-10
EP3702780A1 (en) 2020-09-02
AU2015296551B2 (en) 2021-06-17
EP3982121B1 (en) 2024-04-24
US11732301B2 (en) 2023-08-22
EP3982121A1 (en) 2022-04-13
US20240011089A1 (en) 2024-01-11
JP7320326B2 (en) 2023-08-03
CA3163156A1 (en) 2016-02-04
JP2020030213A (en) 2020-02-27
EP4379373A2 (en) 2024-06-05
JP2017523424A (en) 2017-08-17
AU2021229126A1 (en) 2021-09-30
US20200190577A1 (en) 2020-06-18
US10519499B2 (en) 2019-12-31
EP3702780B1 (en) 2022-03-02
AU2015296551A1 (en) 2017-02-23
CN106796214A (en) 2017-05-31
JP2023164790A (en) 2023-11-14
DK3702780T3 (en) 2022-05-23
CN110954686A (en) 2020-04-03
US20210269877A1 (en) 2021-09-02
JP7010911B2 (en) 2022-01-26
CN113759105A (en) 2021-12-07
EP3175237B1 (en) 2020-03-04
JP2022058596A (en) 2022-04-12
JP6601852B2 (en) 2019-11-06
EP3175237A1 (en) 2017-06-07
US20170260582A1 (en) 2017-09-14
US10961576B2 (en) 2021-03-30
AU2021229125A1 (en) 2021-09-30
CA2956794A1 (en) 2016-02-04

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