DK2814939T3 - Nanoporesensor for enzyme-mediated protein translocation - Google Patents

Nanoporesensor for enzyme-mediated protein translocation Download PDF

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Publication number
DK2814939T3
DK2814939T3 DK13749423.3T DK13749423T DK2814939T3 DK 2814939 T3 DK2814939 T3 DK 2814939T3 DK 13749423 T DK13749423 T DK 13749423T DK 2814939 T3 DK2814939 T3 DK 2814939T3
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protein
nanopore
gly
ala
asp
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DK13749423.3T
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Danish (da)
English (en)
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Jeffrey M Nivala
Douglas B Marks
Mark A Akeson
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Univ California
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721Investigating individual macromolecules, e.g. by translocation through nanopores

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Sustainable Development (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
DK13749423.3T 2012-02-16 2013-02-15 Nanoporesensor for enzyme-mediated protein translocation DK2814939T3 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261599754P 2012-02-16 2012-02-16
US201261713163P 2012-10-12 2012-10-12
PCT/US2013/026414 WO2013123379A2 (en) 2012-02-16 2013-02-15 Nanopore sensor for enzyme-mediated protein translocation

Publications (1)

Publication Number Publication Date
DK2814939T3 true DK2814939T3 (en) 2018-07-30

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DK13749423.3T DK2814939T3 (en) 2012-02-16 2013-02-15 Nanoporesensor for enzyme-mediated protein translocation

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US (2) US11339365B2 (enExample)
EP (1) EP2814939B1 (enExample)
JP (2) JP6312607B2 (enExample)
KR (1) KR102066758B1 (enExample)
CN (1) CN104619854B (enExample)
AU (1) AU2013221356B2 (enExample)
BR (1) BR112014020255B1 (enExample)
CA (1) CA2864824C (enExample)
DK (1) DK2814939T3 (enExample)
WO (1) WO2013123379A2 (enExample)

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BR112014020255B1 (pt) 2022-01-25
US11339365B2 (en) 2022-05-24
US20160032236A1 (en) 2016-02-04
CN104619854B (zh) 2018-07-17
KR102066758B1 (ko) 2020-02-11
JP2018075021A (ja) 2018-05-17
AU2013221356B2 (en) 2018-10-18
EP2814939A2 (en) 2014-12-24
WO2013123379A2 (en) 2013-08-22
BR112014020255A8 (pt) 2017-07-11
KR20140131539A (ko) 2014-11-13
JP6312607B2 (ja) 2018-04-18
CA2864824C (en) 2020-06-16
CN104619854A (zh) 2015-05-13
JP6594944B2 (ja) 2019-10-23
BR112014020255A2 (enExample) 2017-06-20
WO2013123379A3 (en) 2015-03-12
JP2015517799A (ja) 2015-06-25
CA2864824A1 (en) 2013-08-22
EP2814939A4 (en) 2016-02-24
EP2814939B1 (en) 2018-04-18
US20220396758A1 (en) 2022-12-15
AU2013221356A1 (en) 2014-08-28

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