BR112014020255A8 - aparelho, sistema e método de transporte de proteína através de nanoporo - Google Patents
aparelho, sistema e método de transporte de proteína através de nanoporoInfo
- Publication number
- BR112014020255A8 BR112014020255A8 BR112014020255A BR112014020255A BR112014020255A8 BR 112014020255 A8 BR112014020255 A8 BR 112014020255A8 BR 112014020255 A BR112014020255 A BR 112014020255A BR 112014020255 A BR112014020255 A BR 112014020255A BR 112014020255 A8 BR112014020255 A8 BR 112014020255A8
- Authority
- BR
- Brazil
- Prior art keywords
- protein
- nanopore
- unfoldase
- membrane
- face
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Sustainable Development (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261599754P | 2012-02-16 | 2012-02-16 | |
US61/599,754 | 2012-02-16 | ||
US201261713163P | 2012-10-12 | 2012-10-12 | |
US61/713,163 | 2012-10-12 | ||
PCT/US2013/026414 WO2013123379A2 (en) | 2012-02-16 | 2013-02-15 | Nanopore sensor for enzyme-mediated protein translocation |
Publications (3)
Publication Number | Publication Date |
---|---|
BR112014020255A2 BR112014020255A2 (pt) | 2017-06-20 |
BR112014020255A8 true BR112014020255A8 (pt) | 2017-07-11 |
BR112014020255B1 BR112014020255B1 (pt) | 2022-01-25 |
Family
ID=48984893
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112014020255-9A BR112014020255B1 (pt) | 2012-02-16 | 2013-02-15 | Aparelho, sistema e método de transporte de proteína através de nanoporo |
Country Status (10)
Country | Link |
---|---|
US (2) | US11339365B2 (pt) |
EP (1) | EP2814939B1 (pt) |
JP (2) | JP6312607B2 (pt) |
KR (1) | KR102066758B1 (pt) |
CN (1) | CN104619854B (pt) |
AU (1) | AU2013221356B2 (pt) |
BR (1) | BR112014020255B1 (pt) |
CA (1) | CA2864824C (pt) |
DK (1) | DK2814939T3 (pt) |
WO (1) | WO2013123379A2 (pt) |
Families Citing this family (50)
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WO2012138357A1 (en) | 2011-04-04 | 2012-10-11 | President And Fellows Of Harvard College | Nanopore sensing by local electrical potential measurement |
GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
JP6226888B2 (ja) | 2012-02-16 | 2017-11-08 | オックスフォード ナノポール テクノロジーズ リミテッド | ポリマーの測定の解析 |
US9274430B2 (en) | 2012-10-10 | 2016-03-01 | Arizona Board Of Regents On Behalf Of Arizona State University | Systems and devices for molecule sensing and method of manufacturing thereof |
GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
WO2014138253A1 (en) | 2013-03-05 | 2014-09-12 | Arizona Board Of Regents, Acting For And On Behalf Of Arizona State University | Translocation of a polymer through a nanopore |
GB201316849D0 (en) * | 2013-09-23 | 2013-11-06 | Isis Innovation | Method |
US10047392B2 (en) | 2014-02-21 | 2018-08-14 | Northeastern University | Fluorescence-based analysis of biopolymers using nanopores |
WO2015131073A1 (en) | 2014-02-27 | 2015-09-03 | Arizona Board Of Regents Acting For And On Behalf Of Arizona State University | Triazole-based reader molecules and methods for synthesizing and use thereof |
US10519499B2 (en) * | 2014-07-31 | 2019-12-31 | Illumina, Inc. | Hybrid nanopore sensors |
KR102551897B1 (ko) | 2014-10-16 | 2023-07-06 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 폴리머의 분석 |
US20160194698A1 (en) * | 2014-12-16 | 2016-07-07 | Arizona Board Of Regents On Behalf Of Arizona State University | Systems, apparatuses and methods for reading polymer sequence |
EP4293349A3 (en) * | 2015-02-05 | 2024-02-21 | President and Fellows of Harvard College | Nanopore sensor including fluidic passage |
GB201508669D0 (en) | 2015-05-20 | 2015-07-01 | Oxford Nanopore Tech Ltd | Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown |
US10126262B2 (en) * | 2015-09-24 | 2018-11-13 | Genia Technologies, Inc. | Differential output of analog memories storing nanopore measurement samples |
GB201611770D0 (en) | 2016-07-06 | 2016-08-17 | Oxford Nanopore Tech | Microfluidic device |
CN106443008A (zh) * | 2016-08-31 | 2017-02-22 | 中国科学院重庆绿色智能技术研究院 | 一种基于固态纳米孔的hiv‑1蛋白酶检测方法 |
GB201619930D0 (en) * | 2016-11-24 | 2017-01-11 | Oxford Nanopore Tech | Apparatus and methods for controlling insertion of a membrane channel into a membrane |
US11035847B2 (en) * | 2017-06-29 | 2021-06-15 | President And Fellows Of Harvard College | Deterministic stepping of polymers through a nanopore |
WO2019018398A1 (en) * | 2017-07-17 | 2019-01-24 | President And Fellows Of Harvard College | PROTEIN SHUTTLE WITH NANOPORA MATCHING FOR MOLECULAR CHARACTERIZATION |
WO2019081442A1 (en) * | 2017-10-23 | 2019-05-02 | F. Hoffmann-La Roche Ag | REMOVAL AND REINTEGRATION OF PROTEIN NANOPORES IN A MEMBRANE USING AN OSMOTIC IMBALANCE |
CN108226249B (zh) * | 2018-01-09 | 2021-02-26 | 深圳市梅丽纳米孔科技有限公司 | 一次性纳米孔生物传感器及其制作方法 |
CN112534063A (zh) | 2018-05-22 | 2021-03-19 | 安序源有限公司 | 用于核酸测序的方法、系统和组合物 |
WO2020055246A1 (en) | 2018-09-11 | 2020-03-19 | Rijksuniversiteit Groningen | Biological nanopores having tunable pore diameters and uses thereof as analytical tools |
WO2020061385A1 (en) * | 2018-09-19 | 2020-03-26 | Conagen Inc. | Controllable protein degradation via engineering degradation tag variants in corynebacterium host cells |
US20210340192A1 (en) * | 2018-10-05 | 2021-11-04 | University Of Washington | Reporter constructs for nanopore-based detection of biological activity |
WO2020120542A2 (en) * | 2018-12-11 | 2020-06-18 | F. Hoffmann-La Roche Ag | Systems and methods for self-limiting protein pore insertion in a membrane |
WO2020183172A1 (en) | 2019-03-12 | 2020-09-17 | Oxford Nanopore Technologies Inc. | Nanopore sensing device and methods of operation and of forming it |
NL2023062B1 (en) * | 2019-05-03 | 2020-11-30 | Univ Delft Tech | Protein current trace signal acquisition using a nanopore |
GB201907243D0 (en) * | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Sensing interactions between molecular entities and nanapores |
CN112147185B (zh) * | 2019-06-29 | 2022-07-01 | 清华大学 | 一种控制多肽穿过纳米孔速度的方法及其应用 |
GB201915480D0 (en) | 2019-10-25 | 2019-12-11 | Oxford Nanopore Tech Ltd | Improved nanopore sensing device, components and method of manufacture |
WO2021097349A1 (en) * | 2019-11-15 | 2021-05-20 | Machine Bio Inc. | Methods and systems for generating biological molecules |
CA3159307A1 (en) * | 2019-12-02 | 2021-06-10 | Andrew John Heron | Method of characterising a target polypeptide using a nanopore |
NL2024579B1 (en) | 2019-12-24 | 2021-09-06 | Univ Delft Tech | Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes |
CN115398008A (zh) * | 2020-04-14 | 2022-11-25 | 成都今是科技有限公司 | 纳米孔制备和检测方法及其检测装置 |
GB202016874D0 (en) | 2020-10-23 | 2020-12-09 | Oxford Nanopore Tech Ltd | Nanopore support structure and manufacture thereof |
CN115989410A (zh) | 2020-07-17 | 2023-04-18 | 牛津纳米孔科技公开有限公司 | 纳米孔感测装置 |
WO2022020461A1 (en) | 2020-07-22 | 2022-01-27 | Oxford Nanopore Technologies Inc. | Solid state nanopore formation |
JP7450509B2 (ja) | 2020-09-28 | 2024-03-15 | 日立建機株式会社 | ホイール式作業車両 |
US11994508B2 (en) * | 2020-12-23 | 2024-05-28 | Northeastern University | Method and system for linearization and translocation of single protein molecules through nanopores |
US11994484B2 (en) * | 2021-03-25 | 2024-05-28 | The Regents Of The University Of California | Apparatus and method for single cell discrimination |
GB202112235D0 (en) | 2021-08-26 | 2021-10-13 | Oxford Nanopore Tech Ltd | Nanopore |
GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
WO2024030919A1 (en) * | 2022-08-02 | 2024-02-08 | Glyphic Biotechnologies, Inc. | Protein sequencing via coupling of polymerizable molecules |
WO2024091124A1 (en) | 2022-10-28 | 2024-05-02 | Rijksuniversiteit Groningen | Nanopore-based analysis of proteins |
GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
WO2024138570A1 (zh) * | 2022-12-29 | 2024-07-04 | 深圳华大生命科学研究院 | 生物纳米孔传感器、其制备方法及应用 |
WO2024165853A1 (en) | 2023-02-07 | 2024-08-15 | Oxford University Innovation Limited | Method of characterising a peptide, polypeptide or protein using a nanopore |
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US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
SE513280C2 (sv) | 1998-11-05 | 2000-08-14 | Forbo Int Sa | Beläggningsmaterial för golv och förfarande för dess framställning |
US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
US6627067B1 (en) | 1999-06-22 | 2003-09-30 | President And Fellows Of Harvard College | Molecular and atomic scale evaluation of biopolymers |
US6464842B1 (en) | 1999-06-22 | 2002-10-15 | President And Fellows Of Harvard College | Control of solid state dimensional features |
ATE316582T1 (de) | 1999-09-07 | 2006-02-15 | Univ California | Verfahren um die anwesenheit von doppelsträngiger dns in einer probe nachzuweisen |
AU2002239284A1 (en) | 2000-11-27 | 2002-06-03 | The Regents Of The University Of California | Methods and devices for characterizing duplex nucleic acid molecules |
US20030104428A1 (en) | 2001-06-21 | 2003-06-05 | President And Fellows Of Harvard College | Method for characterization of nucleic acid molecules |
US8278055B2 (en) | 2002-05-01 | 2012-10-02 | Intel Corporation | Methods and device for analyte characterization |
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WO2005017025A2 (en) * | 2003-08-15 | 2005-02-24 | The President And Fellows Of Harvard College | Study of polymer molecules and conformations with a nanopore |
WO2006028508A2 (en) | 2004-03-23 | 2006-03-16 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
DE102005013608A1 (de) * | 2005-03-24 | 2006-09-28 | Westfälische Wilhelms-Universität Münster | Vorrichtung und Verfahren für die zellfreie analytische und präparative Proteinsynthese |
CA2684801C (en) | 2007-04-04 | 2017-10-10 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
US8034910B2 (en) | 2008-05-06 | 2011-10-11 | Academia Sinica, Taiwan | SUMO fusion protein expression system for producing native proteins |
GB0820927D0 (en) | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
WO2010082860A1 (en) * | 2009-01-19 | 2010-07-22 | Instituto De Biologia Experimental E Tecnologia (Ibet) | Method and device for nanopore based single-molecule protein/ protein interaction detection |
GB0905140D0 (en) * | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
AU2010240670B2 (en) | 2009-04-20 | 2015-08-20 | Oxford Nanopore Technologies Limited | Lipid bilayer sensor array |
US10385367B2 (en) * | 2009-06-01 | 2019-08-20 | Ginkgo Bioworks, Inc. | Methods and molecules for yield improvement involving metabolic engineering |
US9127313B2 (en) | 2009-12-01 | 2015-09-08 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument |
US9605307B2 (en) | 2010-02-08 | 2017-03-28 | Genia Technologies, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
US8916684B2 (en) * | 2010-06-30 | 2014-12-23 | Syracuse University | Bioengineered protein pores |
-
2013
- 2013-02-15 JP JP2014557827A patent/JP6312607B2/ja active Active
- 2013-02-15 AU AU2013221356A patent/AU2013221356B2/en active Active
- 2013-02-15 CN CN201380019821.3A patent/CN104619854B/zh active Active
- 2013-02-15 KR KR1020147025764A patent/KR102066758B1/ko active IP Right Grant
- 2013-02-15 WO PCT/US2013/026414 patent/WO2013123379A2/en active Application Filing
- 2013-02-15 US US14/378,448 patent/US11339365B2/en active Active
- 2013-02-15 EP EP13749423.3A patent/EP2814939B1/en active Active
- 2013-02-15 CA CA2864824A patent/CA2864824C/en active Active
- 2013-02-15 DK DK13749423.3T patent/DK2814939T3/en active
- 2013-02-15 BR BR112014020255-9A patent/BR112014020255B1/pt active IP Right Grant
-
2017
- 2017-12-08 JP JP2017236383A patent/JP6594944B2/ja active Active
-
2022
- 2022-05-16 US US17/745,399 patent/US20220396758A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US20160032236A1 (en) | 2016-02-04 |
WO2013123379A3 (en) | 2015-03-12 |
CA2864824A1 (en) | 2013-08-22 |
KR102066758B1 (ko) | 2020-02-11 |
JP2018075021A (ja) | 2018-05-17 |
KR20140131539A (ko) | 2014-11-13 |
US20220396758A1 (en) | 2022-12-15 |
CN104619854A (zh) | 2015-05-13 |
AU2013221356A1 (en) | 2014-08-28 |
US11339365B2 (en) | 2022-05-24 |
JP6594944B2 (ja) | 2019-10-23 |
CA2864824C (en) | 2020-06-16 |
BR112014020255B1 (pt) | 2022-01-25 |
CN104619854B (zh) | 2018-07-17 |
EP2814939A2 (en) | 2014-12-24 |
AU2013221356B2 (en) | 2018-10-18 |
BR112014020255A2 (pt) | 2017-06-20 |
EP2814939A4 (en) | 2016-02-24 |
JP2015517799A (ja) | 2015-06-25 |
EP2814939B1 (en) | 2018-04-18 |
JP6312607B2 (ja) | 2018-04-18 |
DK2814939T3 (en) | 2018-07-30 |
WO2013123379A2 (en) | 2013-08-22 |
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Legal Events
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B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
B06T | Formal requirements before examination [chapter 6.20 patent gazette] | ||
B15I | Others concerning applications: loss of priority |
Free format text: PERDA DA PRIORIDADE US 61/599,754 DE 16/02/2012 REIVINDICADA NO PCT/US2013/026414 POR NAO ENVIO DE DOCUMENTO COMPROBATORIO DE CESSAO DA MESMA CONFORME AS DISPOSICOES PREVISTAS NA LEI 9.279 DE 14/05/1996 (LPI) ART. 16 6O, ITEM 27 DO ATO NORMATIVO 128/1997, ART. 28 DA RESOLUCAO INPI-PR 77/2013 E ART 3O DA RESOLUCAO INPI-PR 179 DE 21/02/2017 UMA VEZ QUE DEPOSITANTE CONSTANTE DA PETICAO DE REQUERIMENTO DO PEDIDO PCT E DISTINTO DAQUELE QUE DEPOSITOU A PRIORIDADE REIVINDICADA. |
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B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
B12F | Other appeals [chapter 12.6 patent gazette] |
Free format text: RECURSO: 870210034109 - 15/04/2021 |
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B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
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