BR112022009402A2 - Nanoporos artificiais e usos e métodos relacionados com os mesmos - Google Patents
Nanoporos artificiais e usos e métodos relacionados com os mesmosInfo
- Publication number
- BR112022009402A2 BR112022009402A2 BR112022009402A BR112022009402A BR112022009402A2 BR 112022009402 A2 BR112022009402 A2 BR 112022009402A2 BR 112022009402 A BR112022009402 A BR 112022009402A BR 112022009402 A BR112022009402 A BR 112022009402A BR 112022009402 A2 BR112022009402 A2 BR 112022009402A2
- Authority
- BR
- Brazil
- Prior art keywords
- methods
- nanopores
- artificial
- related uses
- subunit
- Prior art date
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Nanotechnology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
NANOPOROS ARTIFICIAIS E USOS E MÉTODOS RELACIONADOS COM OS MESMOS. A invenção se refere ao campo de nanoporos e seu uso na análise de biopolímeros, incluindo polipeptídeos e polinucleotídeos. É fornecido um nanoporo artificial compreendendo um conjunto multimérico de subunidades, cada subunidade compreendendo (i) a sequência transmembranar (TM) de uma proteína formadora de poros ß-barril ou a-helicoidal fundida com a sequência de aminoácidos de (ii) uma subunidade de uma proteína formadora de anel capaz de controlar o transporte de um polipeptídeo ou polinucleotídeo através da região TM do conjunto.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19210168 | 2019-11-19 | ||
| PCT/NL2020/050726 WO2021101378A1 (en) | 2019-11-19 | 2020-11-19 | Artificial nanopores and uses and methods relating thereto |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112022009402A2 true BR112022009402A2 (pt) | 2022-08-09 |
Family
ID=68840857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112022009402A BR112022009402A2 (pt) | 2019-11-19 | 2020-11-19 | Nanoporos artificiais e usos e métodos relacionados com os mesmos |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US20220412948A1 (pt) |
| EP (1) | EP4061965A1 (pt) |
| JP (1) | JP2023502658A (pt) |
| KR (1) | KR20220100901A (pt) |
| CN (1) | CN114981450A (pt) |
| AU (1) | AU2020389020A1 (pt) |
| BR (1) | BR112022009402A2 (pt) |
| CA (1) | CA3161981A1 (pt) |
| IL (1) | IL293024A (pt) |
| MX (1) | MX2022006018A (pt) |
| WO (1) | WO2021101378A1 (pt) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3111488A1 (en) | 2018-09-11 | 2020-03-19 | Rijksuniversiteit Groningen | Biological nanopores having tunable pore diameters and uses thereof as analytical tools |
| WO2023225988A1 (zh) * | 2022-05-27 | 2023-11-30 | 深圳华大生命科学研究院 | 一种维持纳米孔测序速度的方法 |
| WO2024091124A1 (en) | 2022-10-28 | 2024-05-02 | Rijksuniversiteit Groningen | Nanopore-based analysis of proteins |
| WO2024091123A1 (en) | 2022-10-28 | 2024-05-02 | Rijksuniversiteit Groningen | Nanopore systems and methods for single-molecule polymer profiling |
| CN117417421B (zh) * | 2023-01-12 | 2024-07-23 | 北京普译生物科技有限公司 | 一种突变体膜蛋白复合物纳米孔及其应用 |
| WO2024205413A1 (en) * | 2023-03-30 | 2024-10-03 | Rijksuniversiteit Groningen | Large conical nanopores and uses thereof in analyte sensing |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103695530B (zh) | 2008-07-07 | 2016-05-25 | 牛津纳米孔技术有限公司 | 酶-孔构建体 |
| FR3003268B1 (fr) | 2013-03-13 | 2018-01-19 | Roquette Freres | Bioreacteur |
| JP7237586B2 (ja) * | 2015-12-08 | 2023-03-13 | カトリック ユニヴェルシテット ルーヴェン カーユー ルーヴェン リサーチ アンド ディベロップメント | 修飾ナノポア、それを含む組成物およびその使用 |
| EP3423485B1 (en) * | 2016-03-02 | 2021-12-29 | Oxford Nanopore Technologies plc | Mutant pore |
| CN109890980A (zh) | 2016-07-12 | 2019-06-14 | 格罗宁根大学 | 基于FraC actinoporin的用于生物聚合物传感和测序的生物纳米孔 |
| GB201809323D0 (en) * | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
-
2020
- 2020-11-19 EP EP20815959.0A patent/EP4061965A1/en active Pending
- 2020-11-19 MX MX2022006018A patent/MX2022006018A/es unknown
- 2020-11-19 CN CN202080093474.9A patent/CN114981450A/zh active Pending
- 2020-11-19 IL IL293024A patent/IL293024A/en unknown
- 2020-11-19 KR KR1020227019003A patent/KR20220100901A/ko active Search and Examination
- 2020-11-19 AU AU2020389020A patent/AU2020389020A1/en active Pending
- 2020-11-19 CA CA3161981A patent/CA3161981A1/en active Pending
- 2020-11-19 US US17/777,757 patent/US20220412948A1/en active Pending
- 2020-11-19 BR BR112022009402A patent/BR112022009402A2/pt unknown
- 2020-11-19 JP JP2022529047A patent/JP2023502658A/ja active Pending
- 2020-11-19 WO PCT/NL2020/050726 patent/WO2021101378A1/en active Application Filing
-
2023
- 2023-12-22 US US18/394,833 patent/US20240288416A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR20220100901A (ko) | 2022-07-18 |
| US20220412948A1 (en) | 2022-12-29 |
| CA3161981A1 (en) | 2021-05-27 |
| CN114981450A (zh) | 2022-08-30 |
| JP2023502658A (ja) | 2023-01-25 |
| US20240288416A1 (en) | 2024-08-29 |
| MX2022006018A (es) | 2022-09-12 |
| WO2021101378A1 (en) | 2021-05-27 |
| EP4061965A1 (en) | 2022-09-28 |
| AU2020389020A1 (en) | 2022-06-09 |
| IL293024A (en) | 2022-07-01 |
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