JP2018046876A5 - - Google Patents
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- Publication number
- JP2018046876A5 JP2018046876A5 JP2018000610A JP2018000610A JP2018046876A5 JP 2018046876 A5 JP2018046876 A5 JP 2018046876A5 JP 2018000610 A JP2018000610 A JP 2018000610A JP 2018000610 A JP2018000610 A JP 2018000610A JP 2018046876 A5 JP2018046876 A5 JP 2018046876A5
- Authority
- JP
- Japan
- Prior art keywords
- dna polymerase
- amino acid
- modification
- modified dna
- polymerase according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 20
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 20
- 125000003275 alpha amino acid group Chemical group 0.000 claims 17
- 238000012986 modification Methods 0.000 claims 15
- 230000004048 modification Effects 0.000 claims 15
- 150000001413 amino acids Chemical class 0.000 claims 12
- 238000006467 substitution reaction Methods 0.000 claims 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims 6
- 108060002716 Exonuclease Proteins 0.000 claims 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 4
- 102000013165 exonuclease Human genes 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 3
- 150000007523 nucleic acids Chemical class 0.000 claims 3
- 108020004707 nucleic acids Proteins 0.000 claims 3
- 102200136576 rs560049593 Human genes 0.000 claims 3
- 241001495444 Thermococcus sp. Species 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 102220172328 rs138818878 Human genes 0.000 claims 2
- 229940035893 uracil Drugs 0.000 claims 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 1
- 239000004214 Fast Green FCF Substances 0.000 claims 1
- 229930010555 Inosine Natural products 0.000 claims 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims 1
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 claims 1
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 claims 1
- 230000000593 degrading effect Effects 0.000 claims 1
- 229960003786 inosine Drugs 0.000 claims 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012217475 | 2012-09-28 | ||
| JP2012217474 | 2012-09-28 | ||
| JP2012217474 | 2012-09-28 | ||
| JP2012217475 | 2012-09-28 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013072516A Division JP6478445B2 (ja) | 2012-09-28 | 2013-03-29 | 改変された耐熱性ｄｎａポリメラーゼ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018046876A JP2018046876A (ja) | 2018-03-29 |
| JP2018046876A5 true JP2018046876A5 (cg-RX-API-DMAC7.html) | 2019-01-17 |
| JP6579204B2 JP6579204B2 (ja) | 2019-09-25 |
Family
ID=50784072
Family Applications (8)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013072516A Active JP6478445B2 (ja) | 2012-09-28 | 2013-03-29 | 改変された耐熱性ｄｎａポリメラーゼ |
| JP2013072517A Active JP6478446B2 (ja) | 2012-09-28 | 2013-03-29 | 改変された耐熱性ｄｎａポリメラーゼ |
| JP2013072515A Active JP6478444B2 (ja) | 2012-09-28 | 2013-03-29 | 改変された耐熱性ｄｎａポリメラーゼ |
| JP2017093925A Active JP6536621B2 (ja) | 2012-09-28 | 2017-05-10 | 改変された耐熱性ｄｎａポリメラーゼ |
| JP2017093924A Active JP6579152B2 (ja) | 2012-09-28 | 2017-05-10 | 改変された耐熱性ｄｎａポリメラーゼ |
| JP2018000610A Active JP6579204B2 (ja) | 2012-09-28 | 2018-01-05 | 改変された耐熱性ｄｎａポリメラーゼ |
| JP2019126133A Active JP6904382B2 (ja) | 2012-09-28 | 2019-07-05 | 改変された耐熱性ｄｎａポリメラーゼ |
| JP2021038397A Active JP7044185B2 (ja) | 2012-09-28 | 2021-03-10 | 改変された耐熱性ｄｎａポリメラーゼ |
Family Applications Before (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013072516A Active JP6478445B2 (ja) | 2012-09-28 | 2013-03-29 | 改変された耐熱性ｄｎａポリメラーゼ |
| JP2013072517A Active JP6478446B2 (ja) | 2012-09-28 | 2013-03-29 | 改変された耐熱性ｄｎａポリメラーゼ |
| JP2013072515A Active JP6478444B2 (ja) | 2012-09-28 | 2013-03-29 | 改変された耐熱性ｄｎａポリメラーゼ |
| JP2017093925A Active JP6536621B2 (ja) | 2012-09-28 | 2017-05-10 | 改変された耐熱性ｄｎａポリメラーゼ |
| JP2017093924A Active JP6579152B2 (ja) | 2012-09-28 | 2017-05-10 | 改変された耐熱性ｄｎａポリメラーゼ |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019126133A Active JP6904382B2 (ja) | 2012-09-28 | 2019-07-05 | 改変された耐熱性ｄｎａポリメラーゼ |
| JP2021038397A Active JP7044185B2 (ja) | 2012-09-28 | 2021-03-10 | 改変された耐熱性ｄｎａポリメラーゼ |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US9963686B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2902488B1 (cg-RX-API-DMAC7.html) |
| JP (8) | JP6478445B2 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2014051031A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015019658A1 (ja) * | 2013-08-06 | 2015-02-12 | 東洋紡株式会社 | 核酸増幅法 |
| JP6489017B2 (ja) * | 2013-08-06 | 2019-03-27 | 東洋紡株式会社 | 核酸増幅法 |
| JP2017093392A (ja) * | 2015-11-27 | 2017-06-01 | 東洋紡株式会社 | バイサルファイト処理された核酸増幅用ｄｎａポリメラーゼ |
| US10626383B2 (en) | 2016-01-15 | 2020-04-21 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
| EP3402826A1 (en) | 2016-01-15 | 2018-11-21 | Thermo Fisher Scientific Baltics UAB | Antibodies that bind thermophilic dna polymerases |
| JP6933907B2 (ja) * | 2017-02-24 | 2021-09-08 | シスメックス株式会社 | 核酸増幅方法 |
| WO2019002178A1 (en) * | 2017-06-26 | 2019-01-03 | Thermo Fisher Scientific Baltics Uab | DNA MUTANTS THERMOPHILIC POLYMERASES |
| IL297994A (en) * | 2018-12-05 | 2025-01-01 | Illumina Inc | Polymerases, compounds, and methods of use |
| US11591629B2 (en) * | 2019-12-23 | 2023-02-28 | Cheng-Yao Chen | Method and kit for template-independent nucleic acid synthesis |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69725076T2 (de) | 1996-07-29 | 2004-04-15 | Toyo Boseki K.K. | Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren |
| JP3487394B2 (ja) * | 1996-07-29 | 2004-01-19 | 東洋紡績株式会社 | 改変された耐熱性ｄｎａポリメラーゼおよびその用途 |
| WO1998040496A1 (en) * | 1997-03-12 | 1998-09-17 | The Perkin-Elmer Corporation | Dna polymerases having improved labeled nucleotide incorporation properties |
| AU1239901A (en) | 1999-10-29 | 2001-05-14 | Stratagene | Compositions and methods utilizing dna polymerases |
| JP3891330B2 (ja) * | 2000-05-11 | 2007-03-14 | 東洋紡績株式会社 | 改変された耐熱性ｄｎａポリメラーゼ |
| DE60141097D1 (de) | 2000-05-11 | 2010-03-11 | Toyo Boseki | Modifizierte thermostabile dna polymerase von pyrococcus kodakaraensis |
| DE60231901D1 (de) * | 2001-11-14 | 2009-05-20 | Toyo Boseki | Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon |
| GB0208768D0 (en) * | 2002-04-17 | 2002-05-29 | Univ Newcastle | DNA polymerases |
| WO2004038007A2 (en) | 2002-10-25 | 2004-05-06 | Stratagene | Dna polymerases with reduced base analog detection activity |
| US20040081965A1 (en) * | 2002-10-25 | 2004-04-29 | Stratagene | DNA polymerases with reduced base analog detection activity |
| US8283148B2 (en) * | 2002-10-25 | 2012-10-09 | Agilent Technologies, Inc. | DNA polymerase compositions for quantitative PCR and methods thereof |
| US7960157B2 (en) | 2002-12-20 | 2011-06-14 | Agilent Technologies, Inc. | DNA polymerase blends and uses thereof |
| US7704712B2 (en) * | 2003-03-25 | 2010-04-27 | Stratagene California | DNA polymerase fusions and uses thereof |
| GB0321306D0 (en) * | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| US7704713B2 (en) * | 2004-06-04 | 2010-04-27 | Takara Bio Inc. | Polypeptides having DNA polymerase activity |
| KR100844358B1 (ko) | 2006-10-02 | 2008-07-07 | 한국해양연구원 | 돌연변이 ｄｎａ 중합효소들 및 그의 유전자들 |
| JP2010035533A (ja) * | 2008-08-08 | 2010-02-18 | Canon Inc | メチル化シトシン検出方法 |
| WO2012154934A1 (en) * | 2011-05-11 | 2012-11-15 | New England Biolabs, Inc. | Dna polymerase variants with reduced exonuclease activity and uses thereof |
| JP5765168B2 (ja) * | 2011-09-28 | 2015-08-19 | トヨタ自動車株式会社 | 車両用ベルト式無段変速機の油圧制御装置 |
| JP5461497B2 (ja) * | 2011-09-28 | 2014-04-02 | 本田技研工業株式会社 | 油圧クラッチの初期制御値学習装置および車両 |
| JP5727340B2 (ja) * | 2011-09-28 | 2015-06-03 | 積水化学工業株式会社 | たて管更新方法 |
| JP6601186B2 (ja) * | 2015-11-26 | 2019-11-06 | コニカミノルタ株式会社 | 生体情報検出装置および制御装置 |
| JP7007695B2 (ja) * | 2016-07-04 | 2022-01-25 | 株式会社トキワ | 塗布体付き化粧料容器 |
-
2013
- 2013-03-29 JP JP2013072516A patent/JP6478445B2/ja active Active
- 2013-03-29 JP JP2013072517A patent/JP6478446B2/ja active Active
- 2013-03-29 JP JP2013072515A patent/JP6478444B2/ja active Active
- 2013-09-27 WO PCT/JP2013/076217 patent/WO2014051031A1/ja not_active Ceased
- 2013-09-27 US US14/422,448 patent/US9963686B2/en active Active
- 2013-09-27 EP EP13841851.2A patent/EP2902488B1/en active Active
-
2017
- 2017-05-10 JP JP2017093925A patent/JP6536621B2/ja active Active
- 2017-05-10 JP JP2017093924A patent/JP6579152B2/ja active Active
-
2018
- 2018-01-05 JP JP2018000610A patent/JP6579204B2/ja active Active
-
2019
- 2019-07-05 JP JP2019126133A patent/JP6904382B2/ja active Active
-
2021
- 2021-03-10 JP JP2021038397A patent/JP7044185B2/ja active Active
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