JP2018161129A5 - - Google Patents

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Publication number

JP2018161129A5

JP2018161129A5 JP2018095268A JP2018095268A JP2018161129A5 JP 2018161129 A5 JP2018161129 A5 JP 2018161129A5 JP 2018095268 A JP2018095268 A JP 2018095268A JP 2018095268 A JP2018095268 A JP 2018095268A JP 2018161129 A5 JP2018161129 A5 JP 2018161129A5

Authority

JP

Japan

Prior art keywords

amino acid

seq

nucleic acid

acid sequence

dna polymerase

Prior art date

2018-05-17

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

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• 235000001014 amino acid Nutrition 0.000 claims 29
• 150000001413 amino acids Chemical class 0.000 claims 27
• 125000003275 alpha amino acid group Chemical group 0.000 claims 21
• 238000003199 nucleic acid amplification method Methods 0.000 claims 16
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 13
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 13
• 238000012986 modification Methods 0.000 claims 13
• 230000004048 modification Effects 0.000 claims 13
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 9
• 229920001184 polypeptide Polymers 0.000 claims 9
• 108090000765 processed proteins & peptides Proteins 0.000 claims 9
• 102000004196 processed proteins & peptides Human genes 0.000 claims 9
• 230000003321 amplification Effects 0.000 claims 6
• 238000006243 chemical reaction Methods 0.000 claims 6
• 102000039446 nucleic acids Human genes 0.000 claims 6
• 108020004707 nucleic acids Proteins 0.000 claims 6
• 150000007523 nucleic acids Chemical class 0.000 claims 6
• 108050006400 Cyclin Proteins 0.000 claims 5
• 102000009339 Proliferating Cell Nuclear Antigen Human genes 0.000 claims 5
• 125000000539 amino acid group Chemical group 0.000 claims 5
• 238000001514 detection method Methods 0.000 claims 5
• 230000000694 effects Effects 0.000 claims 5
• ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims 4
• 238000000034 method Methods 0.000 claims 4
• 239000012472 biological sample Substances 0.000 claims 3
• 239000000203 mixture Substances 0.000 claims 3
• 230000007935 neutral effect Effects 0.000 claims 3
• 238000006467 substitution reaction Methods 0.000 claims 3
• 239000004475 Arginine Substances 0.000 claims 2
• 241000894006 Bacteria Species 0.000 claims 2
• 108060002716 Exonuclease Proteins 0.000 claims 2
• 235000004279 alanine Nutrition 0.000 claims 2
• 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 claims 2
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 2
• 239000003153 chemical reaction reagent Substances 0.000 claims 2
• 230000002708 enhancing effect Effects 0.000 claims 2
• 230000001747 exhibiting effect Effects 0.000 claims 2
• 102000013165 exonuclease Human genes 0.000 claims 2
• 241000203069 Archaea Species 0.000 claims 1
• AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 claims 1
• 239000004214 Fast Green FCF Substances 0.000 claims 1
• JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims 1
• 241000205160 Pyrococcus Species 0.000 claims 1
• 241000205188 Thermococcus Species 0.000 claims 1
• 241000700605 Viruses Species 0.000 claims 1
• 210000001124 body fluid Anatomy 0.000 claims 1
• 239000010839 body fluid Substances 0.000 claims 1
• 239000007853 buffer solution Substances 0.000 claims 1
• 210000004027 cell Anatomy 0.000 claims 1
• 210000003608 fece Anatomy 0.000 claims 1
• 229910001425 magnesium ion Inorganic materials 0.000 claims 1
• 238000000746 purification Methods 0.000 claims 1
• 102220172328 rs138818878 Human genes 0.000 claims 1
• 102200136576 rs560049593 Human genes 0.000 claims 1
• 239000000243 solution Substances 0.000 claims 1
• 210000001519 tissue Anatomy 0.000 claims 1
• 235000011178 triphosphate Nutrition 0.000 claims 1
• 239000001226 triphosphate Substances 0.000 claims 1
• UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 1