JP2018011556A - アミラーゼ活性測定試薬及びアミラーゼ活性測定方法 - Google Patents
アミラーゼ活性測定試薬及びアミラーゼ活性測定方法 Download PDFInfo
- Publication number
- JP2018011556A JP2018011556A JP2016143706A JP2016143706A JP2018011556A JP 2018011556 A JP2018011556 A JP 2018011556A JP 2016143706 A JP2016143706 A JP 2016143706A JP 2016143706 A JP2016143706 A JP 2016143706A JP 2018011556 A JP2018011556 A JP 2018011556A
- Authority
- JP
- Japan
- Prior art keywords
- reagent
- amy
- measuring
- activity
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000000694 effects Effects 0.000 title claims abstract description 69
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 66
- 238000005259 measurement Methods 0.000 title abstract description 30
- 238000000691 measurement method Methods 0.000 title abstract description 5
- 239000004382 Amylase Substances 0.000 title description 4
- 102000013142 Amylases Human genes 0.000 title description 4
- 108010065511 Amylases Proteins 0.000 title description 4
- 235000019418 amylase Nutrition 0.000 title description 4
- 238000000034 method Methods 0.000 claims abstract description 37
- 239000000758 substrate Substances 0.000 claims abstract description 22
- 239000002736 nonionic surfactant Substances 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 10
- 108090000790 Enzymes Proteins 0.000 claims abstract description 10
- 125000000217 alkyl group Chemical group 0.000 claims abstract description 8
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 3
- 125000004432 carbon atom Chemical group C* 0.000 claims description 6
- 102100024295 Maltase-glucoamylase Human genes 0.000 claims description 4
- 108010028144 alpha-Glucosidases Proteins 0.000 claims description 4
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 102000004139 alpha-Amylases Human genes 0.000 abstract description 73
- 108090000637 alpha-Amylases Proteins 0.000 abstract description 73
- 229940024171 alpha-amylase Drugs 0.000 abstract description 73
- 230000009257 reactivity Effects 0.000 abstract description 15
- 229940088598 enzyme Drugs 0.000 abstract description 9
- 230000008878 coupling Effects 0.000 abstract 1
- 238000010168 coupling process Methods 0.000 abstract 1
- 238000005859 coupling reaction Methods 0.000 abstract 1
- BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 description 11
- 210000002700 urine Anatomy 0.000 description 11
- 210000002966 serum Anatomy 0.000 description 10
- 238000002835 absorbance Methods 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- 239000004094 surface-active agent Substances 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 210000002381 plasma Anatomy 0.000 description 7
- 239000012190 activator Substances 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
- 239000002585 base Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 3
- 0 *c1c(*)c(I)c(*)c(OCCO)c1* Chemical compound *c1c(*)c(I)c(*)c(OCCO)c1* 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- AJTVSSFTXWNIRG-UHFFFAOYSA-N 2-[bis(2-hydroxyethyl)amino]ethanesulfonic acid Chemical compound OCC[NH+](CCO)CCS([O-])(=O)=O AJTVSSFTXWNIRG-UHFFFAOYSA-N 0.000 description 2
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 description 2
- XCBLFURAFHFFJF-UHFFFAOYSA-N 3-[bis(2-hydroxyethyl)azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCCN(CCO)CC(O)CS(O)(=O)=O XCBLFURAFHFFJF-UHFFFAOYSA-N 0.000 description 2
- 239000007992 BES buffer Substances 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- 206010018910 Haemolysis Diseases 0.000 description 2
- 239000007993 MOPS buffer Substances 0.000 description 2
- JOCBASBOOFNAJA-UHFFFAOYSA-N N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid Chemical compound OCC(CO)(CO)NCCS(O)(=O)=O JOCBASBOOFNAJA-UHFFFAOYSA-N 0.000 description 2
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000007994 TES buffer Substances 0.000 description 2
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 239000006172 buffering agent Substances 0.000 description 2
- 229910001424 calcium ion Inorganic materials 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000008588 hemolysis Effects 0.000 description 2
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 210000003079 salivary gland Anatomy 0.000 description 2
- 238000005464 sample preparation method Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- QZTKDVCDBIDYMD-UHFFFAOYSA-N 2,2'-[(2-amino-2-oxoethyl)imino]diacetic acid Chemical compound NC(=O)CN(CC(O)=O)CC(O)=O QZTKDVCDBIDYMD-UHFFFAOYSA-N 0.000 description 1
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 1
- LVQFQZZGTZFUNF-UHFFFAOYSA-N 2-hydroxy-3-[4-(2-hydroxy-3-sulfonatopropyl)piperazine-1,4-diium-1-yl]propane-1-sulfonate Chemical compound OS(=O)(=O)CC(O)CN1CCN(CC(O)CS(O)(=O)=O)CC1 LVQFQZZGTZFUNF-UHFFFAOYSA-N 0.000 description 1
- 229940100555 2-methyl-4-isothiazolin-3-one Drugs 0.000 description 1
- NUFBIAUZAMHTSP-UHFFFAOYSA-N 3-(n-morpholino)-2-hydroxypropanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CN1CCOCC1 NUFBIAUZAMHTSP-UHFFFAOYSA-N 0.000 description 1
- RZQXOGQSPBYUKH-UHFFFAOYSA-N 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCC(CO)(CO)NCC(O)CS(O)(=O)=O RZQXOGQSPBYUKH-UHFFFAOYSA-N 0.000 description 1
- 229940100484 5-chloro-2-methyl-4-isothiazolin-3-one Drugs 0.000 description 1
- 239000007991 ACES buffer Substances 0.000 description 1
- 239000007988 ADA buffer Substances 0.000 description 1
- 108700016232 Arg(2)-Sar(4)- dermorphin (1-4) Proteins 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- OWXMKDGYPWMGEB-UHFFFAOYSA-N HEPPS Chemical compound OCCN1CCN(CCCS(O)(=O)=O)CC1 OWXMKDGYPWMGEB-UHFFFAOYSA-N 0.000 description 1
- GIZQLVPDAOBAFN-UHFFFAOYSA-N HEPPSO Chemical compound OCCN1CCN(CC(O)CS(O)(=O)=O)CC1 GIZQLVPDAOBAFN-UHFFFAOYSA-N 0.000 description 1
- 108010044467 Isoenzymes Proteins 0.000 description 1
- FSVCELGFZIQNCK-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)glycine Chemical compound OCCN(CCO)CC(O)=O FSVCELGFZIQNCK-UHFFFAOYSA-N 0.000 description 1
- DBXNUXBLKRLWFA-UHFFFAOYSA-N N-(2-acetamido)-2-aminoethanesulfonic acid Chemical compound NC(=O)CNCCS(O)(=O)=O DBXNUXBLKRLWFA-UHFFFAOYSA-N 0.000 description 1
- 239000007990 PIPES buffer Substances 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 description 1
- 239000007997 Tricine buffer Substances 0.000 description 1
- 208000034953 Twin anemia-polycythemia sequence Diseases 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- -1 alkali metal azide Chemical class 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 150000001540 azides Chemical class 0.000 description 1
- 239000007998 bicine buffer Substances 0.000 description 1
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 1
- 239000001639 calcium acetate Substances 0.000 description 1
- 229960005147 calcium acetate Drugs 0.000 description 1
- 235000011092 calcium acetate Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- DHNRXBZYEKSXIM-UHFFFAOYSA-N chloromethylisothiazolinone Chemical compound CN1SC(Cl)=CC1=O DHNRXBZYEKSXIM-UHFFFAOYSA-N 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 1
- 238000003028 enzyme activity measurement method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- GUWHRJQTTVADPB-UHFFFAOYSA-N lithium azide Chemical compound [Li+].[N-]=[N+]=[N-] GUWHRJQTTVADPB-UHFFFAOYSA-N 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- BEGLCMHJXHIJLR-UHFFFAOYSA-N methylisothiazolinone Chemical compound CN1SC=CC1=O BEGLCMHJXHIJLR-UHFFFAOYSA-N 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000000282 nail Anatomy 0.000 description 1
- 210000000944 nerve tissue Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- ZNNZYHKDIALBAK-UHFFFAOYSA-M potassium thiocyanate Chemical compound [K+].[S-]C#N ZNNZYHKDIALBAK-UHFFFAOYSA-M 0.000 description 1
- 229940116357 potassium thiocyanate Drugs 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- VGTPCRGMBIAPIM-UHFFFAOYSA-M sodium thiocyanate Chemical compound [Na+].[S-]C#N VGTPCRGMBIAPIM-UHFFFAOYSA-M 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 210000001138 tear Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
[1] ENMを基質として、α−グルコシターゼを共役酵素として含む、AMY活性測定試薬において、下記一般式(1)
ProClin300:2-メチル-4-イソチアゾリン-3-オンおよび5-クロロ-2-メチル-4-イソチアゾリン-3-オンを有効成分とする防腐剤
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2016143706A JP6891368B2 (ja) | 2016-07-21 | 2016-07-21 | アミラーゼ活性測定試薬及びアミラーゼ活性測定方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2016143706A JP6891368B2 (ja) | 2016-07-21 | 2016-07-21 | アミラーゼ活性測定試薬及びアミラーゼ活性測定方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2018011556A true JP2018011556A (ja) | 2018-01-25 |
JP6891368B2 JP6891368B2 (ja) | 2021-06-18 |
Family
ID=61018842
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2016143706A Active JP6891368B2 (ja) | 2016-07-21 | 2016-07-21 | アミラーゼ活性測定試薬及びアミラーゼ活性測定方法 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP6891368B2 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110951824A (zh) * | 2019-11-15 | 2020-04-03 | 中山市创艺生化工程有限公司 | 一种复合缓冲体系在制备α-淀粉酶测定试剂盒中的应用 |
-
2016
- 2016-07-21 JP JP2016143706A patent/JP6891368B2/ja active Active
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110951824A (zh) * | 2019-11-15 | 2020-04-03 | 中山市创艺生化工程有限公司 | 一种复合缓冲体系在制备α-淀粉酶测定试剂盒中的应用 |
CN110951824B (zh) * | 2019-11-15 | 2023-08-11 | 中山市创艺生化工程有限公司 | 一种复合缓冲体系在制备α-淀粉酶测定试剂盒中的应用 |
Also Published As
Publication number | Publication date |
---|---|
JP6891368B2 (ja) | 2021-06-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP3873222B2 (ja) | 臨床診断システムにおいて促進された性能を有する試薬 | |
JPH02238897A (ja) | 総ビリルビンの測定方法および測定用試薬 | |
CS199692B2 (en) | Method of photometric determination of hydrogen peroxides | |
JP4755802B2 (ja) | クレアチニン・センサーの校正 | |
JP6891368B2 (ja) | アミラーゼ活性測定試薬及びアミラーゼ活性測定方法 | |
JP4639287B2 (ja) | 酵素的測定試薬の安定化方法 | |
JPH01108997A (ja) | 血液又は血液から導出した試料中の血清のフルクトスアミン含量を特異的に測定する方法及び試薬、及び非特異的還元作用を有するか、又は/及び溷濁を惹起する試料成分を除去する方法 | |
JP4022799B2 (ja) | 電解質測定用試薬組成物 | |
JP4130724B2 (ja) | キレート物質を含む測定試薬 | |
JP3403407B2 (ja) | ヒドロキシルアミンまたは酸化防止剤を含有する対照試薬 | |
JP4013108B2 (ja) | リパーゼの安定化方法 | |
JP2000189194A (ja) | α―アミラ―ゼ活性測定試薬及び測定方法 | |
JP2008070346A (ja) | 生体試料中の測定対象物質の測定方法及び測定試薬、ヘモグロビンの影響回避方法、並びにヘモグロビンの影響回避剤 | |
JPH0155880B2 (ja) | ||
JP3760250B2 (ja) | 硫酸抱合型胆汁酸の定量法及びそのキット | |
US6818415B2 (en) | Sodium activation of amylase | |
JP2003000236A (ja) | エステラーゼの安定化方法 | |
US5470715A (en) | Composition for determination of chloride ion | |
JP3448864B2 (ja) | 酵素活性測定用試薬 | |
JP2024005038A (ja) | ヘモグロビンの安定化方法、及びヘモグロビン保存溶液 | |
JP2006129802A (ja) | 電解質測定用組成物 | |
JPH09224697A (ja) | 生体成分の測定方法および測定用組成物 | |
JP3656251B2 (ja) | プロテアーゼの保存方法 | |
JP5808616B2 (ja) | 干渉物質の影響を抑制する方法 | |
EP0886139A1 (en) | A reagent for measurement and a measurement method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20160722 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20190530 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20190530 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20200623 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20200731 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20210406 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20210416 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6891368 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |