JP2017527313A5 - - Google Patents
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- JP2017527313A5 JP2017527313A5 JP2017533733A JP2017533733A JP2017527313A5 JP 2017527313 A5 JP2017527313 A5 JP 2017527313A5 JP 2017533733 A JP2017533733 A JP 2017533733A JP 2017533733 A JP2017533733 A JP 2017533733A JP 2017527313 A5 JP2017527313 A5 JP 2017527313A5
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- polynucleotide
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- 108091033319 polynucleotide Proteins 0.000 claims description 570
- 102000040430 polynucleotide Human genes 0.000 claims description 570
- 239000002157 polynucleotide Substances 0.000 claims description 569
- 238000000034 method Methods 0.000 claims description 444
- 230000000295 complement effect Effects 0.000 claims description 259
- 210000004027 cell Anatomy 0.000 claims description 170
- 239000000203 mixture Substances 0.000 claims description 147
- 239000002773 nucleotide Substances 0.000 claims description 106
- 125000003729 nucleotide group Chemical group 0.000 claims description 106
- 230000001413 cellular effect Effects 0.000 claims description 90
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- 238000003199 nucleic acid amplification method Methods 0.000 claims description 40
- 108060003951 Immunoglobulin Proteins 0.000 claims description 38
- 102000018358 immunoglobulin Human genes 0.000 claims description 38
- 102000039446 nucleic acids Human genes 0.000 claims description 27
- 108020004707 nucleic acids Proteins 0.000 claims description 27
- 150000007523 nucleic acids Chemical class 0.000 claims description 27
- 102100034343 Integrase Human genes 0.000 claims description 24
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 24
- 238000012163 sequencing technique Methods 0.000 claims description 15
- 238000011144 upstream manufacturing Methods 0.000 claims description 13
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims description 11
- 210000004602 germ cell Anatomy 0.000 claims description 11
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- 210000001744 T-lymphocyte Anatomy 0.000 claims description 9
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- 102000016266 T-Cell Antigen Receptors Human genes 0.000 claims description 8
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- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 2
- 108020004635 Complementary DNA Proteins 0.000 claims 1
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 claims 1
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- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical group CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 16
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- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 description 10
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- 229930024421 Adenine Natural products 0.000 description 8
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- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 8
- 239000005547 deoxyribonucleotide Substances 0.000 description 8
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 8
- 229940113082 thymine Drugs 0.000 description 8
- 239000012634 fragment Substances 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 6
- 241000713838 Avian myeloblastosis virus Species 0.000 description 4
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- 230000002934 lysing effect Effects 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
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- 201000009030 Carcinoma Diseases 0.000 description 2
- 208000005243 Chondrosarcoma Diseases 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- 208000032612 Glial tumor Diseases 0.000 description 2
- 206010018338 Glioma Diseases 0.000 description 2
- 206010025323 Lymphomas Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000713869 Moloney murine leukemia virus Species 0.000 description 2
- 206010039491 Sarcoma Diseases 0.000 description 2
- 208000009956 adenocarcinoma Diseases 0.000 description 2
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- 239000000091 biomarker candidate Substances 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
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- 230000037431 insertion Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 208000032839 leukemia Diseases 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 210000001806 memory b lymphocyte Anatomy 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 230000003472 neutralizing effect Effects 0.000 description 2
- 201000008968 osteosarcoma Diseases 0.000 description 2
- 206010041823 squamous cell carcinoma Diseases 0.000 description 2
- 206010044412 transitional cell carcinoma Diseases 0.000 description 2
- 206010069754 Acquired gene mutation Diseases 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 description 1
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 1
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- 238000010257 thawing Methods 0.000 description 1
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Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020036503A JP6960490B2 (ja) | 2014-09-15 | 2020-03-04 | ハイスループットヌクレオチドライブラリーシークエンシング |
| JP2021166730A JP7278352B2 (ja) | 2014-09-15 | 2021-10-11 | ハイスループットヌクレオチドライブラリーシークエンシング |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462050549P | 2014-09-15 | 2014-09-15 | |
| US62/050,549 | 2014-09-15 | ||
| US201462051832P | 2014-09-17 | 2014-09-17 | |
| US62/051,832 | 2014-09-17 | ||
| PCT/US2015/050119 WO2016044227A1 (en) | 2014-09-15 | 2015-09-15 | High-throughput nucleotide library sequencing |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020036503A Division JP6960490B2 (ja) | 2014-09-15 | 2020-03-04 | ハイスループットヌクレオチドライブラリーシークエンシング |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017527313A JP2017527313A (ja) | 2017-09-21 |
| JP2017527313A5 true JP2017527313A5 (enExample) | 2018-11-01 |
| JP6672310B2 JP6672310B2 (ja) | 2020-03-25 |
Family
ID=54291588
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017533733A Active JP6672310B2 (ja) | 2014-09-15 | 2015-09-15 | ハイスループットヌクレオチドライブラリーシークエンシング |
| JP2020036503A Active JP6960490B2 (ja) | 2014-09-15 | 2020-03-04 | ハイスループットヌクレオチドライブラリーシークエンシング |
| JP2021166730A Active JP7278352B2 (ja) | 2014-09-15 | 2021-10-11 | ハイスループットヌクレオチドライブラリーシークエンシング |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020036503A Active JP6960490B2 (ja) | 2014-09-15 | 2020-03-04 | ハイスループットヌクレオチドライブラリーシークエンシング |
| JP2021166730A Active JP7278352B2 (ja) | 2014-09-15 | 2021-10-11 | ハイスループットヌクレオチドライブラリーシークエンシング |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US10590483B2 (enExample) |
| EP (3) | EP3194593B1 (enExample) |
| JP (3) | JP6672310B2 (enExample) |
| KR (1) | KR102541849B1 (enExample) |
| CN (1) | CN107002076B (enExample) |
| AU (2) | AU2015318011B2 (enExample) |
| CA (1) | CA2961210A1 (enExample) |
| ES (2) | ES2727656T3 (enExample) |
| IL (1) | IL251123B (enExample) |
| MX (1) | MX378730B (enExample) |
| NZ (2) | NZ767927A (enExample) |
| SG (2) | SG10201911069WA (enExample) |
| WO (1) | WO2016044227A1 (enExample) |
| ZA (1) | ZA201701916B (enExample) |
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