JP2017520251A5 - - Google Patents
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- JP2017520251A5 JP2017520251A5 JP2016574911A JP2016574911A JP2017520251A5 JP 2017520251 A5 JP2017520251 A5 JP 2017520251A5 JP 2016574911 A JP2016574911 A JP 2016574911A JP 2016574911 A JP2016574911 A JP 2016574911A JP 2017520251 A5 JP2017520251 A5 JP 2017520251A5
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- nucleotide
- nucleotides
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- 239000002773 nucleotide Substances 0.000 claims 30
- 125000003729 nucleotide group Chemical group 0.000 claims 30
- 239000003795 chemical substances by application Substances 0.000 claims 16
- 239000000203 mixture Substances 0.000 claims 15
- 238000000034 method Methods 0.000 claims 13
- 108091034117 Oligonucleotide Proteins 0.000 claims 9
- 230000000295 complement effect Effects 0.000 claims 9
- 239000000523 sample Substances 0.000 claims 9
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 230000002441 reversible effect Effects 0.000 claims 5
- 108090000364 Ligases Proteins 0.000 claims 3
- 102000003960 Ligases Human genes 0.000 claims 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 2
- 230000000415 inactivating effect Effects 0.000 claims 2
- 108091023037 Aptamer Proteins 0.000 claims 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- 239000002751 oligonucleotide probe Substances 0.000 claims 1
- 239000002516 radical scavenger Substances 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021111337A JP2021164471A (ja) | 2014-06-23 | 2021-07-05 | プライマー伸長によるスライドガラス上での染色 |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462015799P | 2014-06-23 | 2014-06-23 | |
| US62/015,799 | 2014-06-23 | ||
| US14/560,921 | 2014-12-04 | ||
| US14/560,921 US9909167B2 (en) | 2014-06-23 | 2014-12-04 | On-slide staining by primer extension |
| PCT/US2015/036763 WO2015200139A1 (en) | 2014-06-23 | 2015-06-19 | On-slide staining by primer extension |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021111337A Division JP2021164471A (ja) | 2014-06-23 | 2021-07-05 | プライマー伸長によるスライドガラス上での染色 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017520251A JP2017520251A (ja) | 2017-07-27 |
| JP2017520251A5 true JP2017520251A5 (show.php) | 2018-07-26 |
| JP6910145B2 JP6910145B2 (ja) | 2021-07-28 |
Family
ID=54869107
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016574911A Active JP6910145B2 (ja) | 2014-06-23 | 2015-06-19 | プライマー伸長によるスライドガラス上での染色 |
| JP2021111337A Pending JP2021164471A (ja) | 2014-06-23 | 2021-07-05 | プライマー伸長によるスライドガラス上での染色 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021111337A Pending JP2021164471A (ja) | 2014-06-23 | 2021-07-05 | プライマー伸長によるスライドガラス上での染色 |
Country Status (7)
| Country | Link |
|---|---|
| US (11) | US9909167B2 (show.php) |
| EP (2) | EP3822362B1 (show.php) |
| JP (2) | JP6910145B2 (show.php) |
| CN (2) | CN106574301B (show.php) |
| AU (2) | AU2015280326B2 (show.php) |
| CA (1) | CA2952872A1 (show.php) |
| WO (1) | WO2015200139A1 (show.php) |
Families Citing this family (109)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10501791B2 (en) | 2011-10-14 | 2019-12-10 | President And Fellows Of Harvard College | Sequencing by structure assembly |
| WO2013067451A2 (en) | 2011-11-04 | 2013-05-10 | Population Diagnostics Inc. | Methods and compositions for diagnosing, prognosing, and treating neurological conditions |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| EP2812452B1 (en) | 2012-02-09 | 2020-05-27 | Population Bio, Inc. | Methods and compositions for screening and treating developmental disorders |
| US10138509B2 (en) | 2013-03-12 | 2018-11-27 | President And Fellows Of Harvard College | Method for generating a three-dimensional nucleic acid containing matrix |
| CN105473744B (zh) | 2013-06-25 | 2020-06-19 | 普罗格诺西斯生物科学公司 | 采用微流控装置的空间编码生物分析 |
| DK3511422T3 (da) | 2013-11-12 | 2023-02-06 | Population Bio Inc | Fremgangsmåder og sammensætninger til diagnosticering, prognose og behandling af endometriose |
| US9909167B2 (en) | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| CN113186256B (zh) | 2015-04-10 | 2025-05-23 | 十程基因技术瑞典公司 | 生物样本的空间区别、多重核酸分析 |
| JP6971218B2 (ja) | 2015-07-17 | 2021-11-24 | ナノストリング テクノロジーズ，インコーポレイティド | 横断組織切片のユーザー定義領域における遺伝子発現の同時定量 |
| AU2016297513B2 (en) | 2015-07-17 | 2021-02-25 | Board Of Regents, The University Of Texas System | Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue |
| DK4015647T3 (da) | 2016-02-26 | 2023-12-04 | Univ Leland Stanford Junior | Multiplexeret enkeltmolekyle-RNA-visualisering med et to-sonde-proximetetsligationssystem |
| CN116200465A (zh) | 2016-04-25 | 2023-06-02 | 哈佛学院董事及会员团体 | 用于原位分子检测的杂交链反应方法 |
| US11512344B2 (en) * | 2016-05-10 | 2022-11-29 | Arizona Board Of Regents On Behalf Of Arizona State University | Consecutive hybridization for multiplexed analysis of biological samples |
| EP3460474B1 (en) * | 2016-05-19 | 2022-04-06 | Toppan Printing Co., Ltd. | Method and kit for target molecule detection |
| EP4050112A1 (en) | 2016-06-21 | 2022-08-31 | 10X Genomics, Inc. | Nucleic acid sequencing |
| EP3490616B1 (en) * | 2016-07-27 | 2022-09-28 | The Board of Trustees of the Leland Stanford Junior University | Highly-multiplexed fluorescent imaging |
| FI3929304T3 (fi) | 2016-12-16 | 2024-03-27 | Aratome Llc | Molekulaarin tunnistaminen käyttämällä ligatiovahvistusta |
| WO2019068880A1 (en) | 2017-10-06 | 2019-04-11 | Cartana Ab | RNA MATRIX LIGATION |
| CA3090000A1 (en) * | 2018-01-30 | 2019-08-08 | Rush University Medical Center | Sequential staining for multiplex analyses of tissues and cells |
| KR20250161650A (ko) | 2018-02-12 | 2025-11-17 | 브루커 스페이셜 바이올로지, 인크. | 유전자 및 단백질 발현을 검출하는 생체 분자 프로브 및 방법 |
| US10871485B2 (en) | 2018-04-13 | 2020-12-22 | Rarecyte, Inc. | Kits for labeling of biomarkers and methods of using the same |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| CN113195735B (zh) | 2018-10-19 | 2025-03-04 | 阿科亚生物科学股份有限公司 | 共存的受体编码核酸区段的检测 |
| JP2022505653A (ja) * | 2018-10-31 | 2022-01-14 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | オリゴヌクレオチドにコンジュゲートされた抗体を用いて細胞を検出するための方法およびキット |
| US12529094B2 (en) | 2018-12-10 | 2026-01-20 | 10X Genomics, Inc. | Imaging system hardware |
| CN113767177B (zh) | 2018-12-10 | 2025-01-14 | 10X基因组学有限公司 | 生成用于空间分析的捕获探针 |
| CN113287014A (zh) * | 2018-12-27 | 2021-08-20 | 生物辐射实验室股份有限公司 | 循序多重蛋白质印迹 |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| BR112021016040A2 (pt) * | 2019-04-25 | 2021-10-05 | Agilent Technologies, Inc. | Composições de hibridização e métodos para preparar e usar composições |
| WO2020243579A1 (en) | 2019-05-30 | 2020-12-03 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| SG11202111878RA (en) | 2019-05-31 | 2021-11-29 | 10X Genomics Inc | Method of detecting target nucleic acid molecules |
| US12281355B2 (en) | 2019-09-30 | 2025-04-22 | Akoya Biosciences, Inc. | Multiplexed imaging with enzyme mediated amplification |
| DK4051810T3 (da) * | 2019-10-28 | 2024-09-09 | Pixelgen Tech Ab | Fremgangsmåde til kortlægning af rullende-cirkel amplifikationsprodukter |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| FI3891300T3 (fi) | 2019-12-23 | 2023-05-10 | 10X Genomics Inc | Menetelmät spatiaalista analyysiä varten rna-templatoitua ligaatiota käyttäen |
| US12241890B2 (en) | 2019-12-23 | 2025-03-04 | 10X Genomics, Inc. | Methods for generating barcoded nucleic acid molecules using fixed cells |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12110548B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Bi-directional in situ analysis |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| US12215379B2 (en) | 2020-02-17 | 2025-02-04 | 10X Genomics, Inc. | In situ analysis of chromatin interaction |
| AU2021225020A1 (en) | 2020-02-21 | 2022-08-18 | 10X Genomics, Inc. | Methods and compositions for integrated in situ spatial assay |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| US12188085B2 (en) | 2020-03-05 | 2025-01-07 | 10X Genomics, Inc. | Three-dimensional spatial transcriptomics with sequencing readout |
| EP4139485B1 (en) | 2020-04-22 | 2023-09-06 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
| EP4153776B1 (en) | 2020-05-22 | 2025-03-05 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| EP4153775B1 (en) | 2020-05-22 | 2024-07-24 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| ES2981265T3 (es) | 2020-06-08 | 2024-10-08 | 10X Genomics Inc | Métodos para determinar un margen quirúrgico y métodos de uso del mismo |
| US12209273B2 (en) | 2020-06-12 | 2025-01-28 | 10X Genomics, Inc. | Nucleic acid assays using click chemistry bioconjugation |
| EP4172362B1 (en) | 2020-06-25 | 2024-09-18 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| AU2021314130A1 (en) | 2020-07-20 | 2023-02-23 | Akoya Biosciences, Inc. | Processing and imaging tissue samples |
| EP4121557A4 (en) | 2020-08-06 | 2024-05-01 | Singular Genomics Systems, Inc. | Spatial sequencing |
| US11492662B2 (en) | 2020-08-06 | 2022-11-08 | Singular Genomics Systems, Inc. | Methods for in situ transcriptomics and proteomics |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| US12297499B2 (en) | 2020-08-17 | 2025-05-13 | 10X Genomics, Inc. | Multicomponent nucleic acid probes for sample analysis |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US12071667B2 (en) | 2020-11-04 | 2024-08-27 | 10X Genomics, Inc. | Sequence analysis using meta-stable nucleic acid molecules |
| WO2022109196A1 (en) | 2020-11-18 | 2022-05-27 | Akoya Biosciences, Inc. | Multiplexed imaging with nanobody probes |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| US12509717B2 (en) | 2020-12-11 | 2025-12-30 | 10X Genomics, Inc. | Methods and compositions for multimodal in situ analysis |
| WO2022140028A1 (en) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| US12060603B2 (en) | 2021-01-19 | 2024-08-13 | 10X Genomics, Inc. | Methods for internally controlled in situ assays using padlock probes |
| US12275984B2 (en) | 2021-03-02 | 2025-04-15 | 10X Genomics, Inc. | Sequential hybridization and quenching |
| EP4305196B1 (en) | 2021-04-14 | 2025-04-02 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| EP4334706A4 (en) | 2021-05-05 | 2025-08-06 | Singular Genomics Systems Inc | MULTIOMICS ANALYSIS DEVICE AND METHODS OF USE THEREOF |
| WO2022236054A1 (en) | 2021-05-06 | 2022-11-10 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| CN113024665A (zh) * | 2021-05-31 | 2021-06-25 | 泛肽生物科技(浙江)有限公司 | 一种oligos标记抗体的方法及其标记得到的抗体 |
| WO2022256503A1 (en) | 2021-06-03 | 2022-12-08 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| WO2023009842A1 (en) | 2021-07-30 | 2023-02-02 | 10X Genomics, Inc. | Methods and compositions for synchronizing reactions in situ |
| US12139751B2 (en) | 2021-07-30 | 2024-11-12 | 10X Genomics, Inc. | Circularizable probes for in situ analysis |
| US12460251B2 (en) | 2021-08-03 | 2025-11-04 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid molecules |
| US12391984B2 (en) | 2021-08-03 | 2025-08-19 | 10X Genomics, Inc. | Compositions and methods for rolling circle amplification |
| US12529096B2 (en) | 2021-08-03 | 2026-01-20 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid structures |
| WO2023023484A1 (en) | 2021-08-16 | 2023-02-23 | 10X Genomics, Inc. | Probes comprising a split barcode region and methods of use |
| WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
| WO2023086880A1 (en) | 2021-11-10 | 2023-05-19 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| EP4305195A2 (en) | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| US12497653B2 (en) | 2022-01-21 | 2025-12-16 | 10X Genomics, Inc. | Multiple readout signals for analyzing a sample |
| CN118974836A (zh) | 2022-03-08 | 2024-11-15 | 10X基因组学有限公司 | 将光学拥挤最小化的原位代码设计方法 |
| WO2023192616A1 (en) | 2022-04-01 | 2023-10-05 | 10X Genomics, Inc. | Compositions and methods for targeted masking of autofluorescence |
| WO2023220300A1 (en) | 2022-05-11 | 2023-11-16 | 10X Genomics, Inc. | Compositions and methods for in situ sequencing |
| EP4540607A1 (en) | 2022-06-17 | 2025-04-23 | 10X Genomics, Inc. | Catalytic de-crosslinking of samples for in situ analysis |
| EP4558819A1 (en) * | 2022-07-20 | 2025-05-28 | President And Fellows Of Harvard College | Method for highly multiplexed, thermal controllable dna extension and its applications |
| CN119677873A (zh) | 2022-08-16 | 2025-03-21 | 10X基因组学有限公司 | Ap50聚合酶及其用途 |
| CN116287135A (zh) * | 2023-01-12 | 2023-06-23 | 华中科技大学 | Rna酶抑制剂在rna原位杂交中的用途 |
| WO2024249961A1 (en) | 2023-06-01 | 2024-12-05 | Singular Genomics Systems, Inc. | Methods and probes for detecting polynucleotide sequences in cells and tissues |
| US12319956B2 (en) | 2023-07-31 | 2025-06-03 | 10X Genomics, Inc. | Methods and systems for targeted RNA cleavage and target RNA-primed rolling circle amplification |
| EP4560319A1 (en) | 2023-11-24 | 2025-05-28 | Pierre Fabre Medicament | Immune and tumour cells expression of vista in a panel of cancer indications |
| WO2025189030A1 (en) | 2024-03-07 | 2025-09-12 | Stellaromics, Inc. | Methods and systems for volumetric imaging |
| CN118496347B (zh) * | 2024-05-30 | 2024-10-25 | 北京航空航天大学杭州创新研究院 | 一种用于抗体寡核苷酸偶联的方法 |
| CN120005963B (zh) * | 2025-02-28 | 2025-11-07 | 青岛农业大学 | 一种快速计数牛奶中菌落总数的方法及染色试剂组合 |
Family Cites Families (122)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1000796A (en) | 1910-11-08 | 1911-08-15 | Jacob Whitfield Graham | Spring-tire. |
| US5063151A (en) * | 1985-09-20 | 1991-11-05 | Biometallics, Inc. | Immunoassay method and kit |
| US5068178A (en) * | 1988-12-06 | 1991-11-26 | Genetic Systems Corporation | Method of enhancing direct immunofluorescence staining of cells |
| US5491224A (en) | 1990-09-20 | 1996-02-13 | Bittner; Michael L. | Direct label transaminated DNA probe compositions for chromosome identification and methods for their manufacture |
| AU3618593A (en) * | 1992-02-04 | 1993-09-01 | E.I. Du Pont De Nemours And Company | Amplification of assay reporters by nucleic acid replication |
| US5985548A (en) * | 1993-02-04 | 1999-11-16 | E. I. Du Pont De Nemours And Company | Amplification of assay reporters by nucleic acid replication |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US6027890A (en) | 1996-01-23 | 2000-02-22 | Rapigene, Inc. | Methods and compositions for enhancing sensitivity in the analysis of biological-based assays |
| US6117631A (en) * | 1996-10-29 | 2000-09-12 | Polyprobe, Inc. | Detection of antigens via oligonucleotide antibody conjugates |
| US20100081134A1 (en) | 1997-07-21 | 2010-04-01 | Mirkin Chad A | Bio-barcode based detection of target analytes |
| US5948902A (en) | 1997-11-20 | 1999-09-07 | South Alabama Medical Science Foundation | Antisense oligonucleotides to human serine/threonine protein phosphatase genes |
| AU3463099A (en) | 1998-03-31 | 1999-10-18 | Tularik Inc. | High throughput assay for detection of mrna in cells |
| US8131053B2 (en) | 1999-01-25 | 2012-03-06 | Amnis Corporation | Detection of circulating tumor cells using imaging flow cytometry |
| US6316230B1 (en) | 1999-08-13 | 2001-11-13 | Applera Corporation | Polymerase extension at 3′ terminus of PNA-DNA chimera |
| US6998239B1 (en) * | 1999-10-12 | 2006-02-14 | Chemocentryx, Inc. | Method for identifying a modulator of the binding of CCX CKR polypeptide to a chemokine |
| CA2413814A1 (en) | 2000-05-11 | 2002-12-12 | Synthes (U.S.A.) | Push-connection for the detachable connection of two bodies, in particular a pedicle screw and a screwdriver |
| US6511809B2 (en) * | 2000-06-13 | 2003-01-28 | E. I. Du Pont De Nemours And Company | Method for the detection of an analyte by means of a nucleic acid reporter |
| US6790636B1 (en) | 2000-06-14 | 2004-09-14 | The United States Of America As Represented By The Department Of Health And Human Services | Rapid fluorescent labeling of tissue for microdissection using fluorescent specific binding agents |
| US6531283B1 (en) * | 2000-06-20 | 2003-03-11 | Molecular Staging, Inc. | Protein expression profiling |
| US6743592B1 (en) | 2000-07-25 | 2004-06-01 | The Trustees Of The University Of Pennsylvania | Methods, systems and kits for immuno-detection of epitopes expressed on molecules |
| US20040038194A1 (en) | 2000-10-06 | 2004-02-26 | Hofmann Scott Daniel | Diagnostic polymerase chain reaction process utilizing simultaneous capture and detection of amplicons |
| US20020072053A1 (en) * | 2000-12-08 | 2002-06-13 | Mcnally Alan J. | Immunoassay based on DNA replication using labeled primer |
| US7846746B2 (en) | 2001-04-10 | 2010-12-07 | Children's Medical Center Corporation | Methods of analysis and labeling of protein-protein interactions |
| EP1270738A1 (de) | 2001-06-18 | 2003-01-02 | chimera biotec GmbH | Verfahren zum Nachweis von Substanzen in Flüssigkeiten |
| US20020197694A1 (en) | 2001-06-20 | 2002-12-26 | Weiping Shao | Conjugates of reduced antibodies and biomolecules |
| US7341831B2 (en) | 2001-07-18 | 2008-03-11 | The Trustees Of The University Of Pennsylvania | Method for immuno-detection of epitopes |
| US20040091857A1 (en) * | 2001-07-20 | 2004-05-13 | Nallur Girish N. | Gene expression profiling |
| US6658780B2 (en) | 2001-09-06 | 2003-12-09 | Aris Papadimas | Revolver device |
| JP3926178B2 (ja) * | 2001-11-02 | 2007-06-06 | 富士通株式会社 | 蛋白質検出デバイス |
| JP2005523688A (ja) * | 2002-01-18 | 2005-08-11 | ブリストル−マイヤーズ スクイブ カンパニー | タンパク質チロシンキナーゼおよび／またはタンパク質チロシンキナーゼ経路と相互作用する化合物の活性を予測するためのポリヌクレオチドおよびポリペプチドの同定 |
| CA2480525A1 (en) | 2002-03-29 | 2003-10-09 | Nugen Technologies, Inc. | Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification |
| DE602004021235D1 (de) * | 2003-02-21 | 2009-07-09 | Geneform Technologies Ltd | Verfahren, kits und reagenzien zur nukleinsäuresequenzierung |
| US20040185453A1 (en) * | 2003-03-21 | 2004-09-23 | Joel Myerson | Affinity based methods for separating homologous parental genetic material and uses thereof |
| AU2004232976B2 (en) * | 2003-04-18 | 2011-02-10 | Becton, Dickinson And Company | Immuno-amplification |
| US7198900B2 (en) | 2003-08-29 | 2007-04-03 | Applera Corporation | Multiplex detection compositions, methods, and kits |
| US7341837B2 (en) * | 2003-09-02 | 2008-03-11 | Lawton Robert L | Soluble analyte detection and amplification |
| WO2005054514A1 (en) * | 2003-12-02 | 2005-06-16 | Agency For Science, Technology And Research | Immuno-amplification rna assay |
| KR100695123B1 (ko) | 2003-12-03 | 2007-03-14 | 삼성전자주식회사 | Ｔｍ에 따라 기판상에 고정화된 프로브 폴리뉴클레오티드군을 2이상 포함하는 폴리뉴클레오티드 어레이 및 그를이용하는 표적 핵산 검출 방법 |
| US20050233340A1 (en) | 2004-04-20 | 2005-10-20 | Barrett Michael T | Methods and compositions for assessing CpG methylation |
| AU2005333156B2 (en) | 2004-11-03 | 2011-05-26 | Iris Molecular Diagnostics, Inc. | Homogeneous analyte detection |
| EP1851331B1 (fr) | 2005-02-25 | 2016-02-24 | Commissariat à l'Énergie Atomique et aux Énergies Alternatives | Procede et dispositif pour separer des cibles moleculaires dans un melange complexe |
| US20070020650A1 (en) * | 2005-04-01 | 2007-01-25 | Avak Kahvejian | Methods for detecting proteins |
| CA2609328A1 (en) | 2005-05-26 | 2006-11-30 | The Trustees Of Boston University | Quantification of nucleic acids and proteins using oligonucleotide mass tags |
| US20070026430A1 (en) | 2005-06-30 | 2007-02-01 | Applera Corporation | Proximity probing of target proteins comprising restriction and/or extension |
| US8530196B2 (en) | 2006-04-07 | 2013-09-10 | The Trustees Of The Univeristy Of Pennsylvania | In situ cloning from pathological tissue specimens |
| CA2656525A1 (en) * | 2006-06-30 | 2008-01-10 | Applera Corporation | Reversible terminator nucleotides and methods of use |
| US20080124735A1 (en) * | 2006-10-16 | 2008-05-29 | Matthias Schuster | Method for detection of one or more CpG positions |
| EP2407558A1 (en) | 2006-10-31 | 2012-01-18 | Noxxon Pharma AG | Methods for the detection of a single- or double-stranded nucleic acid molecule |
| US8305579B2 (en) | 2006-11-16 | 2012-11-06 | Thomas Pirrie Treynor | Sequential analysis of biological samples |
| US9201063B2 (en) | 2006-11-16 | 2015-12-01 | General Electric Company | Sequential analysis of biological samples |
| SE531135C2 (sv) | 2006-12-01 | 2008-12-23 | Alf Maansson | Känslig detektion av gifter, patogener och sjukdomsmarkörer genom aggregation av cytoskeletala filament |
| US8691508B2 (en) | 2006-12-13 | 2014-04-08 | Autogenomics, Inc. | Concurrent analysis of multiple patient samples using solid phase addressable multiplex test with high signal-to-noise ratio |
| CN101210270A (zh) | 2006-12-31 | 2008-07-02 | 陕西北美基因股份有限公司 | 病毒病原高通量快速排查检测方法 |
| GB0703996D0 (en) * | 2007-03-01 | 2007-04-11 | Oxitec Ltd | Nucleic acid detection |
| US8003314B2 (en) | 2007-04-16 | 2011-08-23 | Diagnostic Hybrids, Inc. | Methods for direct fluorescent antibody virus detection in liquids |
| WO2009012220A2 (en) * | 2007-07-17 | 2009-01-22 | Agilent Technologies Inc. | Analyte detection assays |
| WO2009064933A2 (en) | 2007-11-13 | 2009-05-22 | Ikonisys, Inc. | Detection of circulating tumor cells in peripheral blood with an automated scanning fluorescence microscope |
| US20110136116A1 (en) | 2008-04-08 | 2011-06-09 | Cornell University | Detection of target nucleic acid sequences using fluorescence resonance energy transfer |
| US20100021904A1 (en) | 2008-05-21 | 2010-01-28 | Pierce Niles A | Shielded cross-linking probes |
| US8530156B2 (en) | 2008-08-08 | 2013-09-10 | President And Fellows Of Harvard College | Chemically cleavable phosphoramidite linkers for sequencing by ligation |
| EP2320913A4 (en) | 2008-08-09 | 2012-10-03 | Univ Iowa Res Found | NUCLEIC ACID APTAMERS |
| US8262879B2 (en) | 2008-09-03 | 2012-09-11 | Nabsys, Inc. | Devices and methods for determining the length of biopolymers and distances between probes bound thereto |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| US8309306B2 (en) | 2008-11-12 | 2012-11-13 | Nodality, Inc. | Detection composition |
| CN102317779A (zh) | 2008-11-21 | 2012-01-11 | 圣路易大学 | 检测目标物多个表位的生物感应器 |
| DE102008062372B3 (de) | 2008-12-17 | 2010-06-17 | Medizinische Hochschule Hannover | Nachweiskonjugat und Verfahren zur Analyse |
| US8753824B2 (en) | 2009-04-03 | 2014-06-17 | University Of Virginia Patent Foundation | Serial multiple antigen colocalization in paraffin-embedded tissue |
| US9284559B2 (en) | 2009-04-14 | 2016-03-15 | Wake Forest University Health Sciences | Multivalent aptamer complexes |
| CA2760774A1 (en) | 2009-05-05 | 2010-11-11 | Altermune Technologies, Llc | Chemically programmable immunity |
| US9677125B2 (en) | 2009-10-21 | 2017-06-13 | General Electric Company | Detection of plurality of targets in biological samples |
| WO2011143583A1 (en) * | 2010-05-13 | 2011-11-17 | Illumina, Inc. | Binding assays for markers |
| US8658780B2 (en) | 2010-05-18 | 2014-02-25 | California Institute Of Technology | Triggered covalent probes for imaging and silencing genetic expression |
| US20120004132A1 (en) | 2010-07-02 | 2012-01-05 | Affymetrix, Inc. | Detection of Nucleic Acids and Proteins |
| US20130225420A1 (en) | 2010-08-02 | 2013-08-29 | The Regents Of The University Of California | Molecular subtyping of oral squamous cell carcinoma to distinguish a subtype that is unlikely to metastasize |
| US20120034603A1 (en) | 2010-08-06 | 2012-02-09 | Tandem Diagnostics, Inc. | Ligation-based detection of genetic variants |
| WO2012057689A1 (en) * | 2010-10-29 | 2012-05-03 | Ge Healthcare Bio-Sciences Ab | Proximity ligation technology for western blot applications |
| EP2633080B1 (en) | 2010-10-29 | 2018-12-05 | President and Fellows of Harvard College | Method of detecting targets using fluorescently labelled nucleic acid nanotube probes |
| WO2012061444A2 (en) | 2010-11-01 | 2012-05-10 | Hiddessen Amy L | System for forming emulsions |
| US20130035259A1 (en) | 2010-11-22 | 2013-02-07 | The University Of Chicago | Methods and/or Systems Producing and Providing Sets of Oligonucleotide Conjugates for Assays and Detections |
| US20120178081A1 (en) | 2010-12-31 | 2012-07-12 | Affymetrix. Inc. | Methods of Labeling Cells, Labeled Cells, and uses Thereof |
| US20120196294A1 (en) * | 2011-01-17 | 2012-08-02 | Life Technologies Corporation | Workflow for detection of ligands using nucleic acids |
| GB201101621D0 (en) * | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
| US20120252682A1 (en) * | 2011-04-01 | 2012-10-04 | Maples Corporate Services Limited | Methods and systems for sequencing nucleic acids |
| US20140170653A1 (en) * | 2011-04-15 | 2014-06-19 | Life Technologies Corporation | Chemical ligation |
| US8946389B2 (en) | 2011-04-25 | 2015-02-03 | University Of Washington | Compositions and methods for multiplex biomarker profiling |
| GB201107863D0 (en) | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
| GB201108678D0 (en) * | 2011-05-24 | 2011-07-06 | Olink Ab | Multiplexed proximity ligation assay |
| US9938524B2 (en) * | 2011-11-22 | 2018-04-10 | Active Motif, Inc. | Multiplex isolation of protein-associated nucleic acids |
| US10689643B2 (en) * | 2011-11-22 | 2020-06-23 | Active Motif, Inc. | Targeted transposition for use in epigenetic studies |
| ES2952728T3 (es) * | 2011-12-22 | 2023-11-03 | Harvard College | Métodos para la detección de analitos |
| GB201201547D0 (en) | 2012-01-30 | 2012-03-14 | Olink Ab | Method and product |
| US9267168B2 (en) | 2012-06-12 | 2016-02-23 | Pacific Biosciences Of California, Inc. | Methods and compositions for isolating template nucleic acids |
| US9663818B2 (en) | 2012-06-15 | 2017-05-30 | The University Of Chicago | Oligonucleotide-mediated quantitative multiplexed immunoassays |
| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
| WO2014152625A1 (en) | 2013-03-15 | 2014-09-25 | Genapsys, Inc. | Systems and methods for biological analysis |
| JP6073035B2 (ja) | 2013-05-07 | 2017-02-01 | エンパイア テクノロジー ディベロップメント エルエルシー | テラヘルツ周波数タグおよびそれらの作製および使用方法 |
| JP6600302B2 (ja) | 2013-06-12 | 2019-10-30 | ザ ジェネラル ホスピタル コーポレイション | 標的分子のマルチプレックス検出のための方法、キット、およびシステム、ならびにそれらの使用 |
| CN105555972B (zh) * | 2013-07-25 | 2020-07-31 | 伯乐生命医学产品有限公司 | 遗传测定 |
| JP6475718B2 (ja) | 2013-07-30 | 2019-02-27 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 定量的なｄｎａベースのイメージング及び超解像イメージング |
| US9193359B2 (en) | 2013-08-12 | 2015-11-24 | GM Global Technology Operations LLC | Vehicle systems and methods for identifying a driver |
| US20160231324A1 (en) * | 2013-09-24 | 2016-08-11 | The Regents Of The University Of California | Encapsulated sensors and sensing systems for bioassays and diagnostics and methods for making and using them |
| CA2942081C (en) | 2013-10-11 | 2021-09-07 | Ventana Medical Systems, Inc. | Multiplex her2 and estrogen receptor co-staining assays for detecting tumor heterogeneity |
| US9834814B2 (en) | 2013-11-22 | 2017-12-05 | Agilent Technologies, Inc. | Spatial molecular barcoding of in situ nucleic acids |
| GB201401885D0 (en) | 2014-02-04 | 2014-03-19 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (HCR) |
| KR102313982B1 (ko) | 2014-03-11 | 2021-10-18 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 프로그램가능한 핵산 프로브를 이용한 고처리량 고도 다중 영상화 |
| US9753036B2 (en) | 2014-04-29 | 2017-09-05 | Edp Biotech Corporation | Methods and compositions for screening and detecting biomarkers |
| EP3155131B1 (en) | 2014-06-10 | 2020-02-12 | Blueprint Medicines Corporation | Raf1 fusions |
| EP3155426B1 (en) | 2014-06-13 | 2023-07-19 | Immudex ApS | General detection and isolation of specific cells by binding of labeled molecules |
| US9909167B2 (en) | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| RU2715038C2 (ru) | 2014-07-11 | 2020-02-21 | Дженентек, Инк. | Антитела анти-pd-l1 и способы их диагностического применения |
| US9625387B2 (en) | 2014-09-16 | 2017-04-18 | Lawrence Livermore National Security, Llc | System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents |
| US10071121B2 (en) | 2014-11-14 | 2018-09-11 | San Diego State University (Sdsu) Foundation | Cardiac, mesenchymal and endothelial progenitor cell (CPC) chimeras and methods for making and using them |
| US10731202B2 (en) | 2014-11-24 | 2020-08-04 | Battelle Memorial Institute | Chromatin activity precipitation method and system |
| US10006917B2 (en) | 2014-12-15 | 2018-06-26 | President And Fellows Of Harvard College | Methods and compositions relating to super-resolution imaging and modification |
| US20180095067A1 (en) | 2015-04-03 | 2018-04-05 | Abbott Laboratories | Devices and methods for sample analysis |
| CN107636169A (zh) | 2015-04-17 | 2018-01-26 | 生捷科技控股公司 | 对生物分子进行空间概况分析的方法 |
| JP6971218B2 (ja) | 2015-07-17 | 2021-11-24 | ナノストリング テクノロジーズ，インコーポレイティド | 横断組織切片のユーザー定義領域における遺伝子発現の同時定量 |
| KR102557419B1 (ko) | 2015-08-07 | 2023-07-19 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 단백질-단백질 상호작용의 초해상도 영상화 |
| EP4101932A1 (en) | 2016-05-15 | 2022-12-14 | Ultivue, Inc. | Multiplexed imaging using strand displacement |
| US20170349949A1 (en) | 2016-06-03 | 2017-12-07 | Partikula Llc | Methods of selecting subjects for treatment with metabolic modulators |
| EP3490616B1 (en) | 2016-07-27 | 2022-09-28 | The Board of Trustees of the Leland Stanford Junior University | Highly-multiplexed fluorescent imaging |
| US11099170B2 (en) | 2018-06-13 | 2021-08-24 | The Board Of Trustees Of The University Of Illinois | Parallel DNA detection by solid-state multi-pore systems and associated methods |
-
2014
- 2014-12-04 US US14/560,921 patent/US9909167B2/en active Active
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- 2015-06-19 AU AU2015280326A patent/AU2015280326B2/en active Active
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