CN104109194A - Sfrp1蛋白的适配子及其筛选方法和应用 - Google Patents
Sfrp1蛋白的适配子及其筛选方法和应用 Download PDFInfo
- Publication number
- CN104109194A CN104109194A CN201410310259.6A CN201410310259A CN104109194A CN 104109194 A CN104109194 A CN 104109194A CN 201410310259 A CN201410310259 A CN 201410310259A CN 104109194 A CN104109194 A CN 104109194A
- Authority
- CN
- China
- Prior art keywords
- albumen
- aptamer
- sfrp1
- ssdna
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Plant Pathology (AREA)
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201410310259.6A CN104109194B (zh) | 2014-06-30 | 2014-06-30 | Sfrp1蛋白的适配子及其筛选方法和应用 |
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CN201410310259.6A CN104109194B (zh) | 2014-06-30 | 2014-06-30 | Sfrp1蛋白的适配子及其筛选方法和应用 |
Publications (2)
Publication Number | Publication Date |
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CN104109194A true CN104109194A (zh) | 2014-10-22 |
CN104109194B CN104109194B (zh) | 2017-03-15 |
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Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105693847A (zh) * | 2014-07-13 | 2016-06-22 | 马海龙 | 一种特异性针对Neutrokine-α蛋白的核酸适配体NKXA9及其应用 |
CN105693849A (zh) * | 2014-07-13 | 2016-06-22 | 马海龙 | 一种特异性针对Neutrokine-α蛋白的核酸适配体NKXA5及其应用 |
CN105693848A (zh) * | 2014-07-13 | 2016-06-22 | 马海龙 | 一种特异性针对Neutrokine-α蛋白的核酸适配体NKXA7及其应用 |
CN106336454A (zh) * | 2016-11-18 | 2017-01-18 | 李露青 | 人sfrp1变体及其应用 |
CN106349375A (zh) * | 2016-11-17 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349373A (zh) * | 2016-11-17 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349382A (zh) * | 2016-11-21 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349377A (zh) * | 2016-11-18 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349379A (zh) * | 2016-11-22 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349369A (zh) * | 2016-11-15 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349376A (zh) * | 2016-11-18 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349368A (zh) * | 2016-11-15 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349372A (zh) * | 2016-11-16 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349371A (zh) * | 2016-11-16 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349370A (zh) * | 2016-11-16 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349380A (zh) * | 2016-11-22 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349383A (zh) * | 2016-11-21 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349367A (zh) * | 2016-11-15 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349374A (zh) * | 2016-11-17 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349381A (zh) * | 2016-11-21 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
-
2014
- 2014-06-30 CN CN201410310259.6A patent/CN104109194B/zh active Active
Non-Patent Citations (3)
Title |
---|
KALNINE,N. ET AL.: ""Accession NO:AAV38483,secreted frizzled-related protein 1, partial [synthetic construct]]"", 《GENBANK DATABASE》 * |
刘伟伟,万福生: ""核酸适配子在肿瘤诊治中的新进展"", 《肿瘤防治研究》 * |
张孝艳等: ""宫颈癌组织中sFRP1和β-catenin的表达及其意义"", 《中国癌症杂志》 * |
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105693849A (zh) * | 2014-07-13 | 2016-06-22 | 马海龙 | 一种特异性针对Neutrokine-α蛋白的核酸适配体NKXA5及其应用 |
CN105693848A (zh) * | 2014-07-13 | 2016-06-22 | 马海龙 | 一种特异性针对Neutrokine-α蛋白的核酸适配体NKXA7及其应用 |
CN105693847A (zh) * | 2014-07-13 | 2016-06-22 | 马海龙 | 一种特异性针对Neutrokine-α蛋白的核酸适配体NKXA9及其应用 |
CN106349367A (zh) * | 2016-11-15 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349369A (zh) * | 2016-11-15 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349368A (zh) * | 2016-11-15 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349372A (zh) * | 2016-11-16 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349370A (zh) * | 2016-11-16 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349371A (zh) * | 2016-11-16 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349374A (zh) * | 2016-11-17 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349375A (zh) * | 2016-11-17 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349373A (zh) * | 2016-11-17 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349376A (zh) * | 2016-11-18 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349377A (zh) * | 2016-11-18 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106336454A (zh) * | 2016-11-18 | 2017-01-18 | 李露青 | 人sfrp1变体及其应用 |
CN106349383A (zh) * | 2016-11-21 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349382A (zh) * | 2016-11-21 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349381A (zh) * | 2016-11-21 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349379A (zh) * | 2016-11-22 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
CN106349380A (zh) * | 2016-11-22 | 2017-01-25 | 李露青 | 人sfrp1变体及其应用 |
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