JP2016533739A - 変異酵素 - Google Patents

変異酵素 Download PDF

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Publication number
JP2016533739A
JP2016533739A JP2016531773A JP2016531773A JP2016533739A JP 2016533739 A JP2016533739 A JP 2016533739A JP 2016531773 A JP2016531773 A JP 2016531773A JP 2016531773 A JP2016531773 A JP 2016531773A JP 2016533739 A JP2016533739 A JP 2016533739A
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Japan
Prior art keywords
variant
substitution
gh61a
enzyme
amino acid
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JP2016531773A
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Japanese (ja)
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JP2016533739A5 (cg-RX-API-DMAC7.html
Inventor
ダグラス、ニコライ
フォーカラキ、マリア
ホメス、ロナルダス・ウィルヘルムス
ケイパー、タイス
ケレメン、ブラッドリー・アール
クラリュ、スラブコ
ランツ、スザンヌ・イー
ラッシラ、ジョナサン・ケー
ニコラエフ、イゴール
トーレスパズミーノ、ダニエル・エスティバン
デア クレイ、ウィルヘルムス ファン
デア クレイ、ウィルヘルムス ファン
リースハウト、ヨハンネス・フランシスカス・トーマス ファン
リースハウト、ヨハンネス・フランシスカス・トーマス ファン
スタイクト サンズ、サンダー ファン
スタイクト サンズ、サンダー ファン
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ダニスコ・ユーエス・インク
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Publication of JP2016533739A publication Critical patent/JP2016533739A/ja
Publication of JP2016533739A5 publication Critical patent/JP2016533739A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Fodder In General (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2016531773A 2013-07-29 2014-07-24 変異酵素 Pending JP2016533739A (ja)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US201361859630P 2013-07-29 2013-07-29
US201361859735P 2013-07-29 2013-07-29
US201361859666P 2013-07-29 2013-07-29
US201361859712P 2013-07-29 2013-07-29
US201361859704P 2013-07-29 2013-07-29
US201361859721P 2013-07-29 2013-07-29
US201361859680P 2013-07-29 2013-07-29
US61/859,666 2013-07-29
US61/859,735 2013-07-29
US61/859,704 2013-07-29
US61/859,630 2013-07-29
US61/859,712 2013-07-29
US61/859,680 2013-07-29
US61/859,721 2013-07-29
PCT/US2014/048067 WO2015017254A1 (en) 2013-07-29 2014-07-24 Variant enzymes

Publications (2)

Publication Number Publication Date
JP2016533739A true JP2016533739A (ja) 2016-11-04
JP2016533739A5 JP2016533739A5 (cg-RX-API-DMAC7.html) 2017-08-31

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JP2016531773A Pending JP2016533739A (ja) 2013-07-29 2014-07-24 変異酵素
JP2016531774A Pending JP2016533740A (ja) 2013-07-29 2014-07-24 酵素変異体
JP2016531775A Pending JP2016533741A (ja) 2013-07-29 2014-07-24 酵素の変異体

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JP2016531774A Pending JP2016533740A (ja) 2013-07-29 2014-07-24 酵素変異体
JP2016531775A Pending JP2016533741A (ja) 2013-07-29 2014-07-24 酵素の変異体

Country Status (10)

Country Link
US (4) US10081802B2 (cg-RX-API-DMAC7.html)
EP (3) EP3027743A1 (cg-RX-API-DMAC7.html)
JP (3) JP2016533739A (cg-RX-API-DMAC7.html)
CN (3) CN105793417A (cg-RX-API-DMAC7.html)
AU (3) AU2014296574A1 (cg-RX-API-DMAC7.html)
BR (3) BR112016002067A2 (cg-RX-API-DMAC7.html)
DK (2) DK3027742T3 (cg-RX-API-DMAC7.html)
MX (3) MX2016001315A (cg-RX-API-DMAC7.html)
WO (3) WO2015017254A1 (cg-RX-API-DMAC7.html)
ZA (3) ZA201600632B (cg-RX-API-DMAC7.html)

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CN110846332B (zh) * 2019-11-29 2022-05-17 怀化学院 一种果胶酶人工序列及其表达方法和应用
CN110747212B (zh) * 2019-11-29 2022-05-17 怀化学院 一种新果胶酶的基因及其蛋白表达、载体和应用
CN111154743B (zh) * 2020-04-01 2021-01-05 黑龙江省农业科学院畜牧兽医分院 突变型纤维素酶及其在玉米青贮中的应用
EP4355872A2 (en) 2021-06-18 2024-04-24 International N&H Denmark ApS Proteases for beer haze reduction
CN113913412B (zh) * 2021-10-13 2023-10-03 湖北大学 蛋白酶k突变体及其制备方法
CN114480146B (zh) * 2022-04-15 2022-06-24 中国科学院天津工业生物技术研究所 一种米曲霉菌株在富集铜铁锰锌中的应用
JP2025523004A (ja) 2022-07-15 2025-07-17 インターナショナル エヌ アンド エイチ デンマーク エーピーエス 食品中のリン脂質の改善された酵素的改質
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CN117925577B (zh) * 2024-03-19 2024-06-14 金堂佰信维科技有限公司 提高木聚糖酶活性的方法和木聚糖酶及其应用
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