JP2016525366A5 - - Google Patents
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- Publication number
- JP2016525366A5 JP2016525366A5 JP2016530647A JP2016530647A JP2016525366A5 JP 2016525366 A5 JP2016525366 A5 JP 2016525366A5 JP 2016530647 A JP2016530647 A JP 2016530647A JP 2016530647 A JP2016530647 A JP 2016530647A JP 2016525366 A5 JP2016525366 A5 JP 2016525366A5
- Authority
- JP
- Japan
- Prior art keywords
- folate receptor
- amino acid
- sequence
- mutant
- host cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000006815 folate receptor Human genes 0.000 claims description 91
- 108020005243 folate receptor Proteins 0.000 claims description 91
- 239000013604 expression vector Substances 0.000 claims description 59
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 59
- 210000004027 cell Anatomy 0.000 claims description 51
- 150000001413 amino acids Chemical group 0.000 claims description 46
- 235000019152 folic acid Nutrition 0.000 claims description 44
- 239000011724 folic acid Substances 0.000 claims description 44
- 102000040430 polynucleotide Human genes 0.000 claims description 31
- 108091033319 polynucleotide Proteins 0.000 claims description 31
- 239000002157 polynucleotide Substances 0.000 claims description 31
- 229940014144 folate Drugs 0.000 claims description 29
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 24
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims description 24
- 235000001014 amino acid Nutrition 0.000 claims description 24
- 229920001184 polypeptide Polymers 0.000 claims description 24
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 24
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 24
- 235000004279 alanine Nutrition 0.000 claims description 22
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 21
- 239000003550 marker Substances 0.000 claims description 21
- 101001023230 Homo sapiens Folate receptor alpha Proteins 0.000 claims description 17
- 102000053180 human FOLR1 Human genes 0.000 claims description 17
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 16
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 15
- 229960000304 folic acid Drugs 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 14
- 229940024606 amino acid Drugs 0.000 claims description 13
- 230000014509 gene expression Effects 0.000 claims description 12
- 238000006467 substitution reaction Methods 0.000 claims description 9
- 239000004471 Glycine Substances 0.000 claims description 8
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 8
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 8
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims description 8
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 8
- 108010022394 Threonine synthase Proteins 0.000 claims description 8
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims description 8
- 235000003704 aspartic acid Nutrition 0.000 claims description 8
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 8
- 102000004419 dihydrofolate reductase Human genes 0.000 claims description 8
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 8
- 229960000310 isoleucine Drugs 0.000 claims description 8
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims description 8
- 239000004474 valine Substances 0.000 claims description 8
- 230000035899 viability Effects 0.000 claims description 8
- 230000035772 mutation Effects 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 6
- 230000004060 metabolic process Effects 0.000 claims description 4
- ZNOVTXRBGFNYRX-ABLWVSNPSA-N levomefolic acid Chemical compound C1NC=2NC(N)=NC(=O)C=2N(C)C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 ZNOVTXRBGFNYRX-ABLWVSNPSA-N 0.000 claims description 3
- 235000007635 levomefolic acid Nutrition 0.000 claims description 3
- 239000011578 levomefolic acid Substances 0.000 claims description 3
- 102000010451 Folate receptor alpha Human genes 0.000 claims description 2
- 108050001931 Folate receptor alpha Proteins 0.000 claims description 2
- 108060003951 Immunoglobulin Proteins 0.000 claims description 2
- 241000283984 Rodentia Species 0.000 claims description 2
- 239000006143 cell culture medium Substances 0.000 claims description 2
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 2
- 238000000684 flow cytometry Methods 0.000 claims description 2
- 102000018358 immunoglobulin Human genes 0.000 claims description 2
- 210000004962 mammalian cell Anatomy 0.000 claims description 2
- 230000028327 secretion Effects 0.000 claims description 2
- 230000001419 dependent effect Effects 0.000 claims 2
- 239000013598 vector Substances 0.000 claims 2
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 claims 1
- 230000003247 decreasing effect Effects 0.000 claims 1
- 229960002449 glycine Drugs 0.000 claims 1
- 229960003136 leucine Drugs 0.000 claims 1
- 102000005962 receptors Human genes 0.000 claims 1
- 108020003175 receptors Proteins 0.000 claims 1
- 230000003248 secreting effect Effects 0.000 claims 1
- 229960004295 valine Drugs 0.000 claims 1
- 230000000340 anti-metabolite Effects 0.000 description 1
- 229940100197 antimetabolite Drugs 0.000 description 1
- 239000002256 antimetabolite Substances 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361860439P | 2013-07-31 | 2013-07-31 | |
| US61/860,439 | 2013-07-31 | ||
| PCT/IB2014/063517 WO2015015419A1 (en) | 2013-07-31 | 2014-07-29 | Novel selection vectors and methods of selecting eukaryotic host cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016525366A JP2016525366A (ja) | 2016-08-25 |
| JP2016525366A5 true JP2016525366A5 (enExample) | 2017-09-07 |
| JP6576924B2 JP6576924B2 (ja) | 2019-09-18 |
Family
ID=51383909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016530647A Active JP6576924B2 (ja) | 2013-07-31 | 2014-07-29 | 新規の選択ベクターおよび真核宿主細胞を選択する方法 |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US11807855B2 (enExample) |
| EP (3) | EP4095153A1 (enExample) |
| JP (1) | JP6576924B2 (enExample) |
| KR (1) | KR102288233B1 (enExample) |
| CN (2) | CN105593239B (enExample) |
| AU (1) | AU2014297966B2 (enExample) |
| BR (1) | BR112016001182A2 (enExample) |
| CA (1) | CA2918117C (enExample) |
| DK (2) | DK3412684T3 (enExample) |
| ES (2) | ES2689136T3 (enExample) |
| HU (1) | HUE058885T2 (enExample) |
| IL (1) | IL243352B (enExample) |
| LT (2) | LT3027646T (enExample) |
| MX (1) | MX369109B (enExample) |
| PL (2) | PL3412684T3 (enExample) |
| PT (2) | PT3027646T (enExample) |
| RU (1) | RU2716977C2 (enExample) |
| SG (1) | SG11201510625TA (enExample) |
| WO (1) | WO2015015419A1 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2401377T3 (en) | 2009-02-27 | 2016-08-29 | Novartis Ag | Expression vector system comprising two selection markers |
| PT3027646T (pt) | 2013-07-31 | 2018-10-16 | Novartis Ag | Novos vetores e métodos de seleção de células hospedeiras eucarióticas |
| IL258538B2 (en) * | 2015-10-09 | 2023-04-01 | Genzyme Corp | Early isolation after the infection of cells (epic) for the production of biological material |
| EP3521435A1 (en) | 2018-02-06 | 2019-08-07 | Bayer AG | A high stringency selection system for protein expression in proline-auxotrophic cells |
| US20210356377A1 (en) * | 2018-09-07 | 2021-11-18 | Medimmune, Llc | Methods Of Cell Selection |
| MX2022003192A (es) | 2019-09-18 | 2022-04-11 | Novartis Ag | Proteinas de fusion nkg2d y sus usos. |
| JP2023550459A (ja) | 2020-11-23 | 2023-12-01 | ノバルティス アーゲー | 抗体構築物の発現技術 |
| JP2024517711A (ja) | 2021-04-29 | 2024-04-23 | ノバルティス アーゲー | 高シアル酸付加細胞 |
| CN118510902A (zh) * | 2021-12-21 | 2024-08-16 | Absci公司 | 用于鉴定具有增加的可溶性靶蛋白表达的菌株的FolA选择测定 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4771036A (en) | 1986-02-10 | 1988-09-13 | Trustees Of Columbia University In The City Of New York | Method and ophthalmic composition for the prevention and reversal of cataracts |
| JP2585532B2 (ja) | 1986-05-10 | 1997-02-26 | 三井東圧化学株式会社 | 動物細胞の形質転換体及びそれを得る方法並びにその形質転換体を用いてｔ−ＰＡを生産する方法 |
| EP0557459B1 (en) | 1990-11-13 | 1997-10-22 | Immunex Corporation | Bifunctional selectable fusion genes |
| JP3438889B2 (ja) | 1991-03-29 | 2003-08-18 | ジェネンテク，インコーポレイテッド | 所望のタンパク質を高レベルに発現させる組換え宿主細胞の選別法 |
| WO1994028143A1 (en) | 1993-05-21 | 1994-12-08 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
| US5439808A (en) | 1993-07-23 | 1995-08-08 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis |
| US5830698A (en) | 1997-03-14 | 1998-11-03 | Idec Pharmaceuticals Corporation | Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same |
| US6106825A (en) | 1997-05-07 | 2000-08-22 | University Of Florida | Entomopoxvirus-vertebrate gene delivery vector and method |
| FR2784030B1 (fr) | 1998-10-02 | 2002-12-20 | Inst Nat Sante Rech Med | Utilisation de bloqueurs des canaux calciques et/ou cgmp-dependants pour le traitement de pathologies de la retine |
| FR2823221B1 (fr) | 2001-04-06 | 2004-04-02 | Univ Pasteur | Sequences associees a la degenerescence retinienne et applications |
| DE10256083A1 (de) | 2002-11-29 | 2004-08-12 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen |
| US7384744B2 (en) | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
| BRPI0408246A (pt) | 2003-03-11 | 2006-03-01 | Applied Research Systems | vetores de expressão que compreendem o promotor ie2 do mcmv |
| US20070224635A1 (en) | 2004-01-30 | 2007-09-27 | Maxygen Holdings, Ltd. | Regulated Stop Codon Readthrough |
| US20060275794A1 (en) | 2005-03-07 | 2006-12-07 | Invitrogen Corporation | Collections of matched biological reagents and methods for identifying matched reagents |
| CN101389763B (zh) | 2006-02-21 | 2013-01-23 | 科罗迈吉尼科斯公司 | 高水平表达蛋白质的宿主细胞的选择 |
| BRPI0712073B8 (pt) | 2006-05-17 | 2021-05-25 | Hoffmann La Roche | ácido nucléico para produção de um polipeptídio ou proteína heteróloga |
| PL2227549T3 (pl) * | 2007-12-21 | 2015-12-31 | Novartis Ag | System selekcji do hodowli komórek eukariotycznych oparty na genie związanego z błoną receptora folianowego |
| ES2784926T3 (es) | 2007-12-21 | 2020-10-02 | Novartis Ag | Vector de expresión de mamífero |
| RU2528858C2 (ru) | 2008-08-28 | 2014-09-20 | Новартис Аг | Дисплей на поверхности клеток полипептидных изоформ на основе прочитывания терминирующего кодона |
| WO2010029130A1 (en) | 2008-09-10 | 2010-03-18 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Neuronal viability factor and use thereof |
| SG173457A1 (en) | 2009-02-27 | 2011-09-29 | Novartis Ag | Methods for selecting eukaryotic cells expressing a heterologous protein |
| DK2401377T3 (en) | 2009-02-27 | 2016-08-29 | Novartis Ag | Expression vector system comprising two selection markers |
| CN102030754B (zh) * | 2009-09-28 | 2013-07-10 | 中国科学院微生物研究所 | 非病毒载体及其制备方法与应用 |
| PT3027646T (pt) | 2013-07-31 | 2018-10-16 | Novartis Ag | Novos vetores e métodos de seleção de células hospedeiras eucarióticas |
-
2014
- 2014-07-29 PT PT14753158T patent/PT3027646T/pt unknown
- 2014-07-29 EP EP22166577.1A patent/EP4095153A1/en not_active Withdrawn
- 2014-07-29 ES ES14753158.6T patent/ES2689136T3/es active Active
- 2014-07-29 SG SG11201510625TA patent/SG11201510625TA/en unknown
- 2014-07-29 EP EP18179678.0A patent/EP3412684B1/en active Active
- 2014-07-29 PL PL18179678.0T patent/PL3412684T3/pl unknown
- 2014-07-29 KR KR1020167002307A patent/KR102288233B1/ko active Active
- 2014-07-29 US US14/907,866 patent/US11807855B2/en active Active
- 2014-07-29 AU AU2014297966A patent/AU2014297966B2/en active Active
- 2014-07-29 CA CA2918117A patent/CA2918117C/en active Active
- 2014-07-29 PL PL14753158T patent/PL3027646T3/pl unknown
- 2014-07-29 RU RU2016106270A patent/RU2716977C2/ru active
- 2014-07-29 EP EP14753158.6A patent/EP3027646B1/en active Active
- 2014-07-29 MX MX2016001396A patent/MX369109B/es active IP Right Grant
- 2014-07-29 PT PT181796780T patent/PT3412684T/pt unknown
- 2014-07-29 DK DK18179678.0T patent/DK3412684T3/da active
- 2014-07-29 CN CN201480054263.9A patent/CN105593239B/zh active Active
- 2014-07-29 LT LTEP14753158.6T patent/LT3027646T/lt unknown
- 2014-07-29 WO PCT/IB2014/063517 patent/WO2015015419A1/en not_active Ceased
- 2014-07-29 JP JP2016530647A patent/JP6576924B2/ja active Active
- 2014-07-29 DK DK14753158.6T patent/DK3027646T3/en active
- 2014-07-29 LT LTEP18179678.0T patent/LT3412684T/lt unknown
- 2014-07-29 BR BR112016001182A patent/BR112016001182A2/pt not_active Application Discontinuation
- 2014-07-29 ES ES18179678T patent/ES2920510T3/es active Active
- 2014-07-29 HU HUE18179678A patent/HUE058885T2/hu unknown
- 2014-07-29 CN CN202010947233.8A patent/CN112029791A/zh active Pending
-
2015
- 2015-12-24 IL IL243352A patent/IL243352B/en active IP Right Grant
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