JP2016521250A5 - - Google Patents
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- Publication number
- JP2016521250A5 JP2016521250A5 JP2016501834A JP2016501834A JP2016521250A5 JP 2016521250 A5 JP2016521250 A5 JP 2016521250A5 JP 2016501834 A JP2016501834 A JP 2016501834A JP 2016501834 A JP2016501834 A JP 2016501834A JP 2016521250 A5 JP2016521250 A5 JP 2016521250A5
- Authority
- JP
- Japan
- Prior art keywords
- compound
- substrate
- dehalogenase
- mutant
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000001875 compounds Chemical class 0.000 claims description 61
- 239000000758 substrate Substances 0.000 claims description 39
- 238000000034 method Methods 0.000 claims description 23
- 108090000623 proteins and genes Proteins 0.000 claims description 17
- 102000004169 proteins and genes Human genes 0.000 claims description 17
- 125000000524 functional group Chemical group 0.000 claims description 16
- 238000006467 substitution reaction Methods 0.000 claims description 15
- 125000004429 atom Chemical group 0.000 claims description 14
- 239000003814 drug Substances 0.000 claims description 14
- 229940079593 drug Drugs 0.000 claims description 14
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 12
- 239000007787 solid Substances 0.000 claims description 11
- 125000005647 linker group Chemical group 0.000 claims description 10
- 108020001507 fusion proteins Proteins 0.000 claims description 8
- 102000037865 fusion proteins Human genes 0.000 claims description 8
- KXDHJXZQYSOELW-UHFFFAOYSA-N Carbamic acid Chemical group NC(O)=O KXDHJXZQYSOELW-UHFFFAOYSA-N 0.000 claims description 7
- 150000003384 small molecules Chemical class 0.000 claims description 7
- 150000002148 esters Chemical class 0.000 claims description 6
- 125000000217 alkyl group Chemical group 0.000 claims description 4
- 125000000539 amino acid group Chemical group 0.000 claims description 4
- 230000003100 immobilizing effect Effects 0.000 claims description 4
- 238000002372 labelling Methods 0.000 claims description 4
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 claims description 3
- 230000003993 interaction Effects 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 2
- 150000001412 amines Chemical class 0.000 claims description 2
- 239000013592 cell lysate Substances 0.000 claims description 2
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 230000004927 fusion Effects 0.000 claims description 2
- 229910052736 halogen Inorganic materials 0.000 claims description 2
- 125000005843 halogen group Chemical group 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- -1 biomolecule Chemical class 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361788257P | 2013-03-15 | 2013-03-15 | |
| US61/788,257 | 2013-03-15 | ||
| PCT/US2014/025359 WO2014151282A1 (en) | 2013-03-15 | 2014-03-13 | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016521250A JP2016521250A (ja) | 2016-07-21 |
| JP2016521250A5 true JP2016521250A5 (https=) | 2017-04-20 |
| JP6604938B2 JP6604938B2 (ja) | 2019-11-13 |
Family
ID=51580924
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016501834A Active JP6604938B2 (ja) | 2013-03-15 | 2014-03-13 | タンパク質を官能基または固体表面に共有結合的に係留するための基質 |
Country Status (4)
| Country | Link |
|---|---|
| US (3) | US11072812B2 (https=) |
| EP (1) | EP2969435B1 (https=) |
| JP (1) | JP6604938B2 (https=) |
| WO (1) | WO2014151282A1 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2013359160B2 (en) | 2012-12-12 | 2018-11-15 | Promega Corporation | Compositions and methods for capture of cellular targets of bioactive agents |
| US10168323B2 (en) * | 2013-03-15 | 2019-01-01 | Promega Corporation | Compositions and methods for capture of cellular targets of bioactive agents |
| EP2969435B1 (en) | 2013-03-15 | 2021-11-03 | Promega Corporation | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
| JP6876002B2 (ja) | 2015-06-05 | 2021-05-26 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 機能的要素を共有結合により係留させるための細胞透過性、細胞適合性、かつ開裂可能であるリンカー |
| WO2017158136A1 (en) | 2016-03-16 | 2017-09-21 | Oryzon Genomics, S.A. | Methods to determine kdm1a target engagement and chemoprobes useful therefor |
| CN114258321B (zh) * | 2019-03-12 | 2023-09-15 | 诺维卢克斯有限责任公司 | 即时浓度分析仪 |
| EP3941195A4 (en) | 2019-03-20 | 2023-03-22 | Promega Corporation | PHOTOAFFINITY PROBES |
| EP3956352A1 (en) | 2019-04-16 | 2022-02-23 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften E. V. | Circularly permutated haloalkane transferase fusion molecules |
| EP3816180A1 (en) | 2019-10-31 | 2021-05-05 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Circularly permutated haloalkane transferase fusion molecules |
| US12061202B2 (en) | 2019-12-10 | 2024-08-13 | Promega Corporation | Compositions and methods for bioluminescent detection using multifunctional probes |
| JP2025515181A (ja) | 2022-05-04 | 2025-05-13 | プロメガ コーポレイション | 伸長表面ループ領域を有する修飾デハロゲナーゼ |
| WO2023215497A1 (en) | 2022-05-04 | 2023-11-09 | Promega Corporation | Photoactivatable compounds and uses thereof |
| EP4519426A2 (en) | 2022-05-04 | 2025-03-12 | Promega Corporation | Split modified dehalogenase variants |
| US20240060059A1 (en) | 2022-05-04 | 2024-02-22 | Promega Corporation | Circularly permuted dehalogenase variants |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4812409A (en) | 1986-01-31 | 1989-03-14 | Eastman Kodak Company | Hydrolyzable fluorescent substrates and analytical determinations using same |
| US4810636A (en) | 1986-12-09 | 1989-03-07 | Miles Inc. | Chromogenic acridinone enzyme substrates |
| US6162931A (en) | 1996-04-12 | 2000-12-19 | Molecular Probes, Inc. | Fluorinated xanthene derivatives |
| US7879540B1 (en) | 2000-08-24 | 2011-02-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| DK1545613T3 (da) * | 2002-07-31 | 2011-11-14 | Seattle Genetics Inc | Auristatinkonjugater og deres anvendelse til behandling af cancer, en autoimmun sygdom eller en infektiøs sygdom |
| JP4528623B2 (ja) | 2002-09-16 | 2010-08-18 | プロメガ コーポレイション | 迅速分解性レポーター融合タンパク質 |
| EP2341134B1 (en) | 2003-01-31 | 2014-08-27 | Promega Corporation | Covalent tethering of functional groups to proteins |
| US7429472B2 (en) | 2003-01-31 | 2008-09-30 | Promega Corporation | Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule |
| US20050187147A1 (en) | 2003-09-22 | 2005-08-25 | Newman Michael J. | Compositions and methods for increasing drug efficiency |
| CA2541177A1 (en) | 2003-10-03 | 2005-09-22 | Promega Corporation | Vectors for directional cloning |
| US8293503B2 (en) | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
| CA2541765A1 (en) | 2003-10-10 | 2004-10-01 | Promega Corporation | Luciferase biosensor |
| US7553632B2 (en) | 2004-01-22 | 2009-06-30 | Promega Corporation | Luminogenic and nonluminogenic multiplex assay |
| US7416854B2 (en) | 2004-01-22 | 2008-08-26 | Promega Corporation | Luminogenic and nonluminogenic multiplex assay |
| US7425436B2 (en) | 2004-07-30 | 2008-09-16 | Promega Corporation | Covalent tethering of functional groups to proteins and substrates therefor |
| US7728118B2 (en) | 2004-09-17 | 2010-06-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| EP1989548A2 (en) | 2006-02-08 | 2008-11-12 | Promega Corporation | Compositions and methods for capturing and analyzing cross-linked biomolecules |
| EP2327768B1 (en) | 2006-04-03 | 2015-09-09 | Promega Corporation | Permuted and nonpermuted luciferase biosensors |
| EP2087107A2 (en) | 2006-10-30 | 2009-08-12 | Promega Corporation | Mutant hydrolase proteins with enhanced kinetics and functional expression |
| WO2008086035A2 (en) | 2007-01-10 | 2008-07-17 | Promega Corporation | Split mutant hydrolase fusion reporter and uses thereof |
| JPWO2008146685A1 (ja) * | 2007-05-23 | 2010-08-19 | 昭和電工株式会社 | エーテル結合を有する反応性ウレタン化合物、硬化性組成物および硬化物 |
| WO2009142678A1 (en) | 2008-03-27 | 2009-11-26 | Promega Corporatin | Protein labeling with cyanobenzothiazole conjugates |
| JP5597200B2 (ja) * | 2008-08-18 | 2014-10-01 | プロメガ コーポレイション | ルミネッセンスを生じる化合物およびチトクロムｐ４５０３ａ酵素を検出するための方法 |
| LT3409764T (lt) | 2009-05-01 | 2020-06-10 | Promega Corporation | Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga |
| US8309059B2 (en) | 2009-08-31 | 2012-11-13 | Promega Corporation | Reactive cyanine compounds |
| BR112013010487B1 (pt) | 2010-11-02 | 2021-02-02 | Promega Corporation | compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro |
| CA2823837A1 (en) | 2010-12-07 | 2012-06-14 | Yale University | Small-molecule hydrophobic tagging of fusion proteins and induced degradation of same |
| EP2782916B1 (en) | 2011-11-21 | 2018-02-28 | Promega Corporation | Carboxy x rhodamine analogs |
| AU2013359160B2 (en) | 2012-12-12 | 2018-11-15 | Promega Corporation | Compositions and methods for capture of cellular targets of bioactive agents |
| EP2969435B1 (en) | 2013-03-15 | 2021-11-03 | Promega Corporation | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
-
2014
- 2014-03-13 EP EP14769196.8A patent/EP2969435B1/en active Active
- 2014-03-13 US US14/207,993 patent/US11072812B2/en active Active
- 2014-03-13 US US14/207,959 patent/US11072811B2/en active Active
- 2014-03-13 JP JP2016501834A patent/JP6604938B2/ja active Active
- 2014-03-13 WO PCT/US2014/025359 patent/WO2014151282A1/en not_active Ceased
-
2021
- 2021-07-13 US US17/374,460 patent/US12221648B2/en active Active
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