JP2015533079A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2015533079A5 JP2015533079A5 JP2015527512A JP2015527512A JP2015533079A5 JP 2015533079 A5 JP2015533079 A5 JP 2015533079A5 JP 2015527512 A JP2015527512 A JP 2015527512A JP 2015527512 A JP2015527512 A JP 2015527512A JP 2015533079 A5 JP2015533079 A5 JP 2015533079A5
- Authority
- JP
- Japan
- Prior art keywords
- lysate
- droplet
- reagent
- nucleic acid
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 34
- 239000006166 lysate Substances 0.000 claims 26
- 239000003153 chemical reaction reagent Substances 0.000 claims 20
- 108091033319 polynucleotide Proteins 0.000 claims 20
- 102000040430 polynucleotide Human genes 0.000 claims 20
- 239000002157 polynucleotide Substances 0.000 claims 20
- 230000003321 amplification Effects 0.000 claims 18
- 238000003199 nucleic acid amplification method Methods 0.000 claims 18
- 102000004190 Enzymes Human genes 0.000 claims 15
- 108090000790 Enzymes Proteins 0.000 claims 15
- 238000006243 chemical reaction Methods 0.000 claims 14
- 238000001668 nucleic acid synthesis Methods 0.000 claims 14
- 108091005804 Peptidases Proteins 0.000 claims 12
- 239000004365 Protease Substances 0.000 claims 12
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 12
- 235000019419 proteases Nutrition 0.000 claims 12
- 230000000694 effects Effects 0.000 claims 11
- 239000000047 product Substances 0.000 claims 8
- 230000002194 synthesizing effect Effects 0.000 claims 5
- 239000007795 chemical reaction product Substances 0.000 claims 4
- 239000002612 dispersion medium Substances 0.000 claims 4
- 239000012530 fluid Substances 0.000 claims 4
- 230000000415 inactivating effect Effects 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 239000000758 substrate Substances 0.000 claims 4
- 108010067770 Endopeptidase K Proteins 0.000 claims 3
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 239000013592 cell lysate Substances 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 230000002934 lysing effect Effects 0.000 claims 2
- 230000009089 cytolysis Effects 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 238000010839 reverse transcription Methods 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261682707P | 2012-08-13 | 2012-08-13 | |
| US61/682,707 | 2012-08-13 | ||
| US201361784754P | 2013-03-14 | 2013-03-14 | |
| US61/784,754 | 2013-03-14 | ||
| PCT/US2013/054517 WO2014028378A2 (en) | 2012-08-13 | 2013-08-12 | Methods and systems for detecting biological components |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015533079A JP2015533079A (ja) | 2015-11-19 |
| JP2015533079A5 true JP2015533079A5 (enExample) | 2016-10-27 |
| JP6514105B2 JP6514105B2 (ja) | 2019-05-15 |
Family
ID=50101583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015527512A Active JP6514105B2 (ja) | 2012-08-13 | 2013-08-12 | 生物学的成分を検出するための方法およびシステム |
Country Status (8)
| Country | Link |
|---|---|
| US (7) | US10161007B2 (enExample) |
| EP (2) | EP4001426A1 (enExample) |
| JP (1) | JP6514105B2 (enExample) |
| CN (2) | CN110643681A (enExample) |
| AU (3) | AU2013302867A1 (enExample) |
| CA (1) | CA2881783A1 (enExample) |
| GB (2) | GB2539836B (enExample) |
| WO (1) | WO2014028378A2 (enExample) |
Families Citing this family (177)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2539836B (en) | 2012-08-13 | 2017-03-29 | Univ California | Methods for detecting target nucleic acids in sample lysate droplets |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9731293B2 (en) * | 2012-10-03 | 2017-08-15 | The United States Of America, As Represented By The Secretary Of The Navy | Paired laser and electrokinetic separation, manipulation, and analysis device |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| BR112015019159A2 (pt) | 2013-02-08 | 2017-07-18 | 10X Genomics Inc | geração de código de barras de polinucleotídeos |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
| NL2012483B1 (en) * | 2014-03-20 | 2016-01-18 | Stichting Incas3 | Method and system for mapping a three-dimensional structure using motes. |
| DE102014205531B4 (de) * | 2014-03-25 | 2025-01-09 | Robert Bosch Gmbh | Mikrofluidische Vorrichtung und Verfahren zum Analysieren einer Probe biologischen Materials |
| WO2015189336A1 (en) * | 2014-06-11 | 2015-12-17 | Samplix S.A.R.L. | Nucleotide sequence exclusion enrichment by droplet sorting (needls) |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| CN113249435B (zh) | 2014-06-26 | 2024-09-03 | 10X基因组学有限公司 | 分析来自单个细胞或细胞群体的核酸的方法 |
| JP2017522866A (ja) | 2014-06-26 | 2017-08-17 | １０エックス ジェノミクス， インコーポレイテッド | 核酸配列の分析 |
| EP3160654A4 (en) * | 2014-06-27 | 2017-11-15 | The Regents of The University of California | Pcr-activated sorting (pas) |
| DK3208335T3 (da) * | 2014-10-14 | 2020-08-31 | Mgi Tech Co Ltd | Fremgangsmåde til brydning af nukleinsyre og tilføjelse af adapter ved hjælp af transposase og reagens |
| ES2856733T3 (es) * | 2014-10-17 | 2021-09-28 | Ecole Polytech | Procedimiento de manipulación de microgotas que incluye muestras |
| US10434507B2 (en) | 2014-10-22 | 2019-10-08 | The Regents Of The University Of California | High definition microdroplet printer |
| WO2016090148A1 (en) | 2014-12-03 | 2016-06-09 | IsoPlexis Corporation | Analysis and screening of cell secretion profiles |
| WO2016109939A1 (zh) * | 2015-01-06 | 2016-07-14 | 深圳市海普洛斯生物科技有限公司 | 一种富集循环肿瘤dna的方法和试剂 |
| SG11201705615UA (en) | 2015-01-12 | 2017-08-30 | 10X Genomics Inc | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
| EP3253888B1 (en) * | 2015-01-21 | 2021-03-03 | Agency For Science, Technology And Research | Single cell rna and mutational analysis pcr (scrm-pcr): a method for simultaneous analysis of dna and rna at the single-cell level |
| EP3253910B1 (en) * | 2015-02-04 | 2021-07-14 | The Regents of The University of California | Multiple-emulsion nucleic acid amplification |
| US11111519B2 (en) | 2015-02-04 | 2021-09-07 | The Regents Of The University Of California | Sequencing of nucleic acids via barcoding in discrete entities |
| US11634707B2 (en) | 2015-02-10 | 2023-04-25 | Illumina, Inc. | Methods and compositions for analyzing cellular components |
| CN105936930A (zh) * | 2015-03-04 | 2016-09-14 | 松下知识产权经营株式会社 | Dna检测方法和dna检测装置 |
| CN106032551B (zh) * | 2015-03-17 | 2020-07-21 | 东源生物医药科技（上海）有限公司 | 检测病毒样品中回复突变的方法和用于该方法的试剂盒 |
| EP3271713B1 (en) * | 2015-03-18 | 2021-05-05 | The Broad Institute, Inc. | Massively parallel on-chip coalescence of microemulsions |
| JP7144934B2 (ja) * | 2015-03-25 | 2022-09-30 | ザ ジェネラル ホスピタル コーポレイション | 血液試料中の循環腫瘍細胞のデジタル分析 |
| CN108026494A (zh) | 2015-06-05 | 2018-05-11 | 米罗库鲁斯公司 | 限制蒸发和表面结垢的空气基质数字微流控装置和方法 |
| CN208562324U (zh) | 2015-06-05 | 2019-03-01 | 米罗库鲁斯公司 | 空气基质数字微流控(dmf)装置 |
| WO2017004250A1 (en) | 2015-06-29 | 2017-01-05 | Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University | Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis |
| CN107615041B (zh) * | 2015-10-07 | 2020-12-11 | Afi技术公司 | 检查装置、检查系统以及检查方法 |
| AU2016335375A1 (en) * | 2015-10-09 | 2018-04-26 | Sysmex Corporation | Analyte treatment chip, analyte treatment device, and analyte treatment method |
| JP6704920B2 (ja) * | 2015-10-14 | 2020-06-03 | アルプスアルパイン株式会社 | 流路構造体および測定対象液体の測定装置 |
| US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
| JP2017118859A (ja) * | 2015-12-31 | 2017-07-06 | ローラス株式会社 | 滅菌型液体成分検査装置及び滅菌型液体成分検査装置の使用方法 |
| CN105665049B (zh) * | 2016-01-28 | 2017-07-04 | 清华大学深圳研究生院 | 一种疏液微阀式微量液体提取装置和提取方法 |
| US11081208B2 (en) | 2016-02-11 | 2021-08-03 | 10X Genomics, Inc. | Systems, methods, and media for de novo assembly of whole genome sequence data |
| JP6765826B2 (ja) * | 2016-03-10 | 2020-10-07 | シスメックス株式会社 | 検体処理方法、検体処理チップおよび検体処理装置 |
| JP6654951B2 (ja) | 2016-03-31 | 2020-02-26 | 株式会社エンプラス | 流体取扱装置 |
| AU2017297416A1 (en) | 2016-07-12 | 2019-01-31 | Northeastern University | Single cell fluorescence in situ hybridization in microfluidic droplets |
| WO2018017794A1 (en) * | 2016-07-21 | 2018-01-25 | President And Fellows Of Harvard College | Microfluidic sequencing techniques |
| US11142791B2 (en) | 2016-08-10 | 2021-10-12 | The Regents Of The University Of California | Combined multiple-displacement amplification and PCR in an emulsion microdroplet |
| CN106442443B (zh) * | 2016-09-12 | 2018-12-07 | 北京天健惠康生物科技有限公司 | 一种微液滴荧光检测系统 |
| WO2018049418A1 (en) | 2016-09-12 | 2018-03-15 | IsoPlexis Corporation | System and methods for multiplexed analysis of cellular and other immunotherapeutics |
| EP3985127A1 (en) | 2016-10-27 | 2022-04-20 | The General Hospital Corporation | Digital analysis of blood samples to determine efficacy of cancer therapies for specific cancers |
| EP3321352A1 (en) * | 2016-11-09 | 2018-05-16 | Biomillenia SAS | Auxotrophic selection system |
| EP4036578A1 (en) * | 2016-11-11 | 2022-08-03 | Isoplexis Corporation | Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells |
| CN110226084A (zh) | 2016-11-22 | 2019-09-10 | 伊索普莱克西斯公司 | 用于细胞捕获的系统、装置和方法，以及其制造方法 |
| JP2020500517A (ja) | 2016-11-28 | 2020-01-16 | アリゾナ ボード オブ リージェンツ オン ビハーフ オブ アリゾナ ステート ユニバーシティ | 連続流小滴反応に関連するシステムおよび方法 |
| CN108148744A (zh) * | 2016-12-06 | 2018-06-12 | 中山百慧生物科技有限公司 | 一种液滴数字pcr芯片及相应检测方法和检测系统 |
| WO2018119301A1 (en) | 2016-12-21 | 2018-06-28 | The Regents Of The University Of California | Single cell genomic sequencing using hydrogel based droplets |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP3563151A4 (en) | 2016-12-28 | 2020-08-19 | Miroculus Inc. | DIGITAL MICROFLUIDIC DEVICES AND METHODS |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| CN106644900B (zh) * | 2017-02-27 | 2023-10-03 | 大连海事大学 | 一种基于非均匀电场的阻抗脉冲颗粒计数装置及其计数方法 |
| JP2018162990A (ja) * | 2017-03-24 | 2018-10-18 | 株式会社エンプラス | 液体取扱装置、液体取扱方法および液体取扱システム |
| US11213824B2 (en) | 2017-03-29 | 2022-01-04 | The Research Foundation For The State University Of New York | Microfluidic device and methods |
| WO2018187476A1 (en) | 2017-04-04 | 2018-10-11 | Miroculus Inc. | Digital microfluidic apparatuses and methods for manipulating and processing encapsulated droplets |
| US11131673B2 (en) | 2017-04-27 | 2021-09-28 | Northeastern University | Live single-cell bioassay in microdroplets |
| CN107219194B (zh) * | 2017-05-03 | 2019-07-02 | 哈尔滨工业大学 | 具备应激响应的图案化微纳米结构的聚电解质复合物的制备方法及应用 |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| EP4435113B1 (en) | 2017-05-18 | 2025-12-10 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| US20210208045A9 (en) * | 2017-06-12 | 2021-07-08 | Biomillenia Sas | Method Of Measuring Viscosity In A Microfluidic System |
| EP3421131A1 (en) * | 2017-06-30 | 2019-01-02 | Blink AG | A sample cartridge for incubating and/or analyzing a dispersion of particles, cells or droplets |
| CN109201128A (zh) * | 2017-07-05 | 2019-01-15 | 京东方科技集团股份有限公司 | 微流控芯片、化学发光免疫分析系统和分析方法 |
| KR101897250B1 (ko) * | 2017-07-11 | 2018-09-10 | 광주과학기술원 | 항암제 조합에 대한 단일 세포 분석 칩 |
| WO2019023133A1 (en) | 2017-07-24 | 2019-01-31 | Miroculus Inc. | DIGITAL MICROFLUIDIC SYSTEMS AND METHODS WITH INTEGRATED PLASMA COLLECTION DEVICE |
| US11590495B2 (en) | 2017-07-28 | 2023-02-28 | Hewlett-Packard Development Company, L.P. | Ionic species interrogation and sensing |
| US20210246488A1 (en) | 2017-08-14 | 2021-08-12 | Mission Bio, Inc. | Method and compositions for evaluating emulsion uniformity |
| US10821442B2 (en) | 2017-08-22 | 2020-11-03 | 10X Genomics, Inc. | Devices, systems, and kits for forming droplets |
| EP4494757A3 (en) | 2017-09-29 | 2025-03-19 | The Regents of The University of California | Method of generating monodisperse emulsions |
| US10837047B2 (en) | 2017-10-04 | 2020-11-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| US20190112655A1 (en) * | 2017-10-18 | 2019-04-18 | Mission Bio, Inc. | Method, Systems and Apparatus for High-Throughput Single-Cell DNA Sequencing With Droplet Microfluidics |
| US10501739B2 (en) | 2017-10-18 | 2019-12-10 | Mission Bio, Inc. | Method, systems and apparatus for single cell analysis |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS |
| CN111479631B (zh) | 2017-10-27 | 2022-02-22 | 10X基因组学有限公司 | 用于样品制备和分析的方法和系统 |
| CN109746062B (zh) * | 2017-11-06 | 2024-04-12 | 北京新羿生物科技有限公司 | 微液滴生成装置 |
| CN109746058B (zh) * | 2017-11-06 | 2024-05-24 | 北京新羿生物科技有限公司 | 微液滴检测芯片 |
| EP3954782A1 (en) | 2017-11-15 | 2022-02-16 | 10X Genomics, Inc. | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| WO2019108851A1 (en) | 2017-11-30 | 2019-06-06 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation and analysis |
| US11707736B2 (en) * | 2017-12-08 | 2023-07-25 | Enuvio Inc. | Microfluidic chip and method for making the same |
| CN111699388B (zh) | 2017-12-12 | 2024-08-02 | 10X基因组学有限公司 | 用于单细胞处理的系统和方法 |
| CN107868752B (zh) * | 2017-12-19 | 2024-01-05 | 广东顺德墨赛生物科技有限公司 | 夹具 |
| WO2019126789A1 (en) | 2017-12-22 | 2019-06-27 | 10X Genomics, Inc. | Systems and methods for processing nucleic acid molecules from one or more cells |
| CN110064451B (zh) * | 2018-01-24 | 2023-08-15 | 思纳福(苏州)生命科技有限公司 | 流体驱动机构及流体驱动方法 |
| WO2019148013A2 (en) * | 2018-01-29 | 2019-08-01 | The Texas A&M University System | Integrated modular on-chip droplet microfluidic screening platform |
| US11052407B1 (en) * | 2018-01-29 | 2021-07-06 | The Texas A&M University System | Dielectrophoretic in-droplet material concentrator |
| SG11202007686VA (en) | 2018-02-12 | 2020-09-29 | 10X Genomics Inc | Methods characterizing multiple analytes from individual cells or cell populations |
| US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
| WO2019169028A1 (en) | 2018-02-28 | 2019-09-06 | 10X Genomics, Inc. | Transcriptome sequencing through random ligation |
| JP2019170363A (ja) * | 2018-03-29 | 2019-10-10 | 東ソー株式会社 | 標的分子の反応装置及び反応方法 |
| WO2019195197A1 (en) | 2018-04-02 | 2019-10-10 | Dropworks, Inc. | Systems and methods for serial flow emulsion processes |
| EP3775271B1 (en) | 2018-04-06 | 2025-03-12 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| CN112469504B (zh) | 2018-05-23 | 2024-08-16 | 米罗库鲁斯公司 | 对数字微流控中的蒸发的控制 |
| AU2019277193A1 (en) * | 2018-05-28 | 2020-10-29 | AI Fluidics Pty Ltd | Method and apparatus for controlling and manipulation of multi-phase flow in microfluidics using artificial intelligence |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| CN210427605U (zh) * | 2018-06-07 | 2020-04-28 | 洛阳华清天木生物科技有限公司 | 一种微液滴处理装置 |
| US11703427B2 (en) | 2018-06-25 | 2023-07-18 | 10X Genomics, Inc. | Methods and systems for cell and bead processing |
| CN109145713B (zh) * | 2018-07-02 | 2021-09-28 | 南京师范大学 | 一种结合目标检测的小目标语义分割方法 |
| US12188014B1 (en) | 2018-07-25 | 2025-01-07 | 10X Genomics, Inc. | Compositions and methods for nucleic acid processing using blocking agents |
| US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| CN112703252B (zh) | 2018-08-03 | 2024-09-10 | 10X基因组学有限公司 | 用于最小化条形码交换的方法和系统 |
| EP3837377A4 (en) | 2018-08-17 | 2022-05-18 | The Regents of University of California | DROPLET FORMATION TRIGGERED BY MONODISPERSE PARTICLES FROM STEADY JETS |
| US12065688B2 (en) | 2018-08-20 | 2024-08-20 | 10X Genomics, Inc. | Compositions and methods for cellular processing |
| WO2020041148A1 (en) | 2018-08-20 | 2020-02-27 | 10X Genomics, Inc. | Methods and systems for detection of protein-dna interactions using proximity ligation |
| CA3113526A1 (en) | 2018-09-20 | 2020-03-26 | Cepheid | System, device and methods of sample processing using semiconductor detection chips |
| DK3856404T3 (da) | 2018-09-28 | 2024-10-21 | Fluent Biosciences Inc | Indfangning af mål i monodisperse dråber |
| CN109107624A (zh) * | 2018-10-16 | 2019-01-01 | 长春技特生物技术有限公司 | 一种全封闭微流控芯片和乳液微滴制备系统 |
| US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
| CA3123229A1 (en) * | 2018-12-14 | 2020-06-18 | Cepheid | Diagnostic detection chip devices and methods of manufacture and assembly |
| CN109632437B (zh) * | 2018-12-14 | 2020-07-24 | 北京化工大学 | 一种基于离子迁移谱的现场检测的样品前处理方法 |
| US12169198B2 (en) | 2019-01-08 | 2024-12-17 | 10X Genomics, Inc. | Systems and methods for sample analysis |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| WO2020150746A1 (en) * | 2019-01-18 | 2020-07-23 | Mission Bio | Method, apparatus and system to detect sub-particle flowrate in a closed systems |
| CN113543883A (zh) * | 2019-01-31 | 2021-10-22 | 米罗库鲁斯公司 | 非结垢组合物以及用于操控和处理包封的微滴的方法 |
| US12305239B2 (en) | 2019-02-12 | 2025-05-20 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| WO2020167866A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transposon loading |
| EP3924505B1 (en) | 2019-02-12 | 2025-12-17 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| WO2020167862A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transfer of reagents between droplets |
| US12275993B2 (en) | 2019-02-12 | 2025-04-15 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
| US11655499B1 (en) | 2019-02-25 | 2023-05-23 | 10X Genomics, Inc. | Detection of sequence elements in nucleic acid molecules |
| US11920183B2 (en) | 2019-03-11 | 2024-03-05 | 10X Genomics, Inc. | Systems and methods for processing optically tagged beads |
| EP3953041A4 (en) | 2019-04-08 | 2023-01-25 | Miroculus Inc. | MULTI-CARTRIDGE DIGITAL MICROFLUID DEVICES AND METHODS OF USE |
| US12465911B2 (en) | 2019-05-02 | 2025-11-11 | The Regents Of The University Of California | Laser-induced confocal microscope and microfluidic device for dielectrophoretic fluorescence-activated droplet sorting |
| WO2020232072A1 (en) | 2019-05-14 | 2020-11-19 | The Regents Of The University Of California | Platform for the deterministic assembly of microfluidic droplets |
| CA3138806A1 (en) | 2019-05-22 | 2020-11-26 | Dalia Dhingra | Method and apparatus for simultaneous targeted sequencing of dna, rna and protein |
| WO2021003255A1 (en) | 2019-07-01 | 2021-01-07 | Mission Bio | Method and apparatus to normalize quantitative readouts in single-cell experiments |
| AU2020325026A1 (en) | 2019-08-05 | 2022-03-17 | Mission Bio, Inc. | Method and apparatus for single-cell analysis for determining a cell trajectory |
| AU2020327987A1 (en) * | 2019-08-12 | 2022-03-10 | Mission Bio, Inc. | Method, system and apparatus for multi-omic simultaneous detection of protein expression, single nucleotide variations, and copy number variations in the same single cells |
| CN112439467B (zh) * | 2019-08-29 | 2025-05-30 | 上海欣戈赛生物科技有限公司 | 用于制备乳化液滴的芯片及装置 |
| US12235262B1 (en) | 2019-09-09 | 2025-02-25 | 10X Genomics, Inc. | Methods and systems for single cell protein analysis |
| GB201914266D0 (en) * | 2019-10-03 | 2019-11-20 | Univ Oxford Innovation Ltd | Method of amplifying nucleic acids |
| CN114829626A (zh) | 2019-10-10 | 2022-07-29 | 1859公司 | 用于微流体筛选的方法和系统 |
| WO2021112841A1 (en) | 2019-12-04 | 2021-06-10 | Hewlett-Packard Development Company, L.P. | Sorting a droplet including a biologic sample |
| CN115516367B (zh) | 2019-12-10 | 2025-11-11 | 康泰博实验室公司 | 用于三维光片成像的方法和系统 |
| WO2021126969A1 (en) | 2019-12-17 | 2021-06-24 | The Regents Of The University Of California | Selective and high-resolution printing of single cells |
| WO2021146171A1 (en) * | 2020-01-13 | 2021-07-22 | Fluent Biosciences Inc. | Devices for generating monodisperse droplets from a bulk liquid |
| LT6852B (lt) * | 2020-01-27 | 2021-09-27 | Innovation Fort Ltd | Didelio lygiagretumo spartusis ląstelių skaitytuvas ir rūšiuoklis |
| US12449419B1 (en) | 2020-02-12 | 2025-10-21 | 10X Genomics, Inc. | Methods for detecting binding of peptide-MHC monomers to T cells |
| WO2021168768A1 (zh) * | 2020-02-28 | 2021-09-02 | 京东方科技集团股份有限公司 | 一种微流控芯片和微流控系统 |
| WO2021188889A1 (en) | 2020-03-20 | 2021-09-23 | Mission Bio, Inc. | Single cell workflow for whole genome amplification |
| CN111378562A (zh) * | 2020-03-20 | 2020-07-07 | 中国科学院长春光学精密机械与物理研究所 | 数字pcr检测定量系统 |
| US11851700B1 (en) | 2020-05-13 | 2023-12-26 | 10X Genomics, Inc. | Methods, kits, and compositions for processing extracellular molecules |
| EP3925702A1 (en) * | 2020-06-15 | 2021-12-22 | Biomillenia SAS | Method and system of producing a library of microorganisms |
| WO2021263008A1 (en) | 2020-06-26 | 2021-12-30 | The Regents Of The University Of California | Selective addition of reagents to droplets |
| CN116171379A (zh) * | 2020-08-21 | 2023-05-26 | 流体分析有限公司 | 用于分析组分的h-型过滤器装置 |
| WO2022072363A1 (en) * | 2020-09-29 | 2022-04-07 | The Regents Of The University Of California | Method for rapid and large-scale generation of droplets and droplet libraries |
| US12084715B1 (en) | 2020-11-05 | 2024-09-10 | 10X Genomics, Inc. | Methods and systems for reducing artifactual antisense products |
| US12480158B1 (en) | 2020-11-05 | 2025-11-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN116802314A (zh) * | 2020-12-25 | 2023-09-22 | Tl 基因体科技株式会社 | 每个单细胞的特定核酸的拷贝数的检测方法 |
| US12398262B1 (en) | 2021-01-22 | 2025-08-26 | 10X Genomics, Inc. | Triblock copolymer-based cell stabilization and fixation system and methods of use thereof |
| WO2022182682A1 (en) | 2021-02-23 | 2022-09-01 | 10X Genomics, Inc. | Probe-based analysis of nucleic acids and proteins |
| CN117241878A (zh) | 2021-03-05 | 2023-12-15 | 伊努梅里斯公司 | 用于生成微滴和执行数字分析的系统和方法 |
| US20220371018A1 (en) * | 2021-05-18 | 2022-11-24 | Canon Medical Systems Corporation | Droplet collection unit, and droplet collection apparatus and method |
| US12252745B2 (en) | 2021-09-02 | 2025-03-18 | Enumerix, Inc. | Detection and digital quantitation of multiple targets |
| CN114113314B (zh) * | 2021-11-25 | 2024-03-22 | 厦门大学 | 一种用于滨海核电站的海洋致灾生物水声监测系统及方法 |
| EP4453246A1 (en) | 2021-12-20 | 2024-10-30 | Enumerix, Inc. | Detection and digital quantitation of multiple targets |
| US11772093B2 (en) | 2022-01-12 | 2023-10-03 | Miroculus Inc. | Methods of mechanical microfluidic manipulation |
| KR102682799B1 (ko) * | 2022-03-30 | 2024-07-09 | 광운대학교 산학협력단 | 3차원 전극 기반 미세유체 측정장치 및 그 제조방법 |
| CN114921532A (zh) * | 2022-04-29 | 2022-08-19 | 深圳大学 | 一种以非疾病诊断为目的的细胞定量及基因分型方法 |
| EP4331724A1 (en) * | 2022-08-29 | 2024-03-06 | Institut Curie | Method of extracting a bead from a droplet |
| EP4494762A1 (en) * | 2023-07-21 | 2025-01-22 | Blink AG | Methods and devices for processing microparticles |
| CN117925391A (zh) * | 2024-03-06 | 2024-04-26 | 东北林业大学 | 一种基于三内核双乳液滴可控融合的单细胞pcr装置及运行方法 |
Family Cites Families (121)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5733994A (en) | 1992-11-30 | 1994-06-22 | Wellcome Foundation Limited, The | Sequential targeting of tumor sites with oligonucleotide conjugates of antibody and complementary oligonucleotide conjugates of chelated radionuclide |
| US20020068357A1 (en) | 1995-09-28 | 2002-06-06 | Mathies Richard A. | Miniaturized integrated nucleic acid processing and analysis device and method |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| US6319507B1 (en) | 1997-05-02 | 2001-11-20 | Kobo Products, Inc. | Agar gel bead composition and method |
| DK1801214T3 (da) | 1997-07-07 | 2011-01-24 | Medical Res Council | In vitro sorteringsfremgangsmåde |
| US6436677B1 (en) | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
| WO2003054524A1 (en) | 2001-12-11 | 2003-07-03 | Sau Lan Tang Staats | Microfluidic devices and methods for two-dimensional separations |
| JP2006507921A (ja) | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 流体分散のための方法および装置 |
| CA2513535C (en) | 2003-01-29 | 2012-06-12 | 454 Corporation | Bead emulsion nucleic acid amplification |
| US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
| US10533998B2 (en) | 2008-07-18 | 2020-01-14 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| DE10339452A1 (de) | 2003-08-22 | 2005-03-17 | Institut für Physikalische Hochtechnologie e.V. | Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten |
| AU2003903295A0 (en) | 2003-06-30 | 2003-07-10 | Raustech Pty Ltd | Substrate for combinatorial chemistry |
| EP2662136A3 (en) | 2003-08-27 | 2013-12-25 | President and Fellows of Harvard College | Method for handling and mixing droplets |
| US20050069938A1 (en) | 2003-09-26 | 2005-03-31 | Youxiang Wang | Amplification of polynucleotides by rolling circle amplification |
| EP1691792A4 (en) | 2003-11-24 | 2008-05-28 | Yeda Res & Dev | COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES |
| KR100647282B1 (ko) | 2003-12-24 | 2006-11-17 | 삼성전자주식회사 | ＤＮＡ 마이크로어레이 스폿팅(Ｓｐｏｔｉｎｇ) 장치 및이를 이용한 스폿팅 방법 |
| US20060201868A1 (en) | 2005-03-11 | 2006-09-14 | Simmons Blake A | Methods and devices for high-throughput dielectrophoretic concentration |
| WO2007024914A2 (en) | 2005-08-22 | 2007-03-01 | Applera Corporation | Device and method for microfluidic control of a first fluid in contact with a second fluid, wherein the first and second fluids are immiscible |
| US8383059B2 (en) | 2005-09-30 | 2013-02-26 | University Of Utah Research Foundation | Microfluidic interface for highly parallel addressing of sensing arrays |
| US9183349B2 (en) * | 2005-12-16 | 2015-11-10 | Nextbio | Sequence-centric scientific information management |
| EP3913375A1 (en) | 2006-01-11 | 2021-11-24 | Bio-Rad Laboratories, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
| US7998708B2 (en) * | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| EP2021113A2 (en) | 2006-05-11 | 2009-02-11 | Raindance Technologies, Inc. | Microfluidic devices |
| CA2653321A1 (en) | 2006-05-26 | 2007-12-06 | Althea Technologies, Inc. | Biochemical analysis of partitioned cells |
| EP2027470B1 (en) | 2006-06-02 | 2012-11-21 | Pfizer Products Inc. | Circulating tumor cell assay |
| EP2092068B1 (en) * | 2006-12-14 | 2014-10-08 | Dow AgroSciences LLC | Optimized non-canonical zinc finger proteins |
| US8338166B2 (en) | 2007-01-04 | 2012-12-25 | Lawrence Livermore National Security, Llc | Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture |
| WO2008109176A2 (en) | 2007-03-07 | 2008-09-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| WO2009015296A1 (en) | 2007-07-24 | 2009-01-29 | The Regents Of The University Of California | Microfabricated dropley generator |
| US8268564B2 (en) | 2007-09-26 | 2012-09-18 | President And Fellows Of Harvard College | Methods and applications for stitched DNA barcodes |
| GB0720202D0 (en) | 2007-10-16 | 2007-11-28 | Cambridge Entpr Ltd | Microfluidic systems |
| WO2009092106A1 (en) | 2008-01-18 | 2009-07-23 | The Regents Of The University Of California | Accurate and rapid micromixer for integrated microfluidic devices |
| DK2271657T3 (en) | 2008-03-04 | 2017-05-15 | Crystal Bioscience Inc | GEL-MICRO-DROP COMPOSITION AND METHOD OF USING IT |
| US9409177B2 (en) | 2008-03-21 | 2016-08-09 | Lawrence Livermore National Security, Llc | Chip-based device for parallel sorting, amplification, detection, and identification of nucleic acid subsequences |
| US8852952B2 (en) * | 2008-05-03 | 2014-10-07 | Advanced Liquid Logic, Inc. | Method of loading a droplet actuator |
| WO2011120024A1 (en) | 2010-03-25 | 2011-09-29 | Quantalife, Inc. | Droplet generation for droplet-based assays |
| US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
| US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| US20130084572A1 (en) | 2011-09-30 | 2013-04-04 | Quantalife, Inc. | Calibrations and controls for droplet-based assays |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ．ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| CN107267596A (zh) | 2009-06-15 | 2017-10-20 | 考利达基因组股份有限公司 | 用于长片段阅读测序的方法和组合物 |
| JP5841937B2 (ja) | 2009-06-26 | 2016-01-13 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 流体注入 |
| CA3021714C (en) | 2009-09-02 | 2021-03-09 | Bio-Rad Laboratories, Inc. | System for mixing fluids by coalescence of multiple emulsions |
| US8746285B2 (en) | 2009-09-04 | 2014-06-10 | Auburn University | Programmable fluidic droplet generation |
| US9625454B2 (en) * | 2009-09-04 | 2017-04-18 | The Research Foundation For The State University Of New York | Rapid and continuous analyte processing in droplet microfluidic devices |
| US9376713B2 (en) | 2009-09-23 | 2016-06-28 | The Board Of Trustees Of The University Of Illinois | Label free detection of nucleic acid amplification |
| US9890408B2 (en) | 2009-10-15 | 2018-02-13 | Ibis Biosciences, Inc. | Multiple displacement amplification |
| WO2011066476A1 (en) * | 2009-11-25 | 2011-06-03 | Quantalife, Inc. | Methods and compositions for detecting genetic material |
| US9315857B2 (en) | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US20120270739A1 (en) | 2010-01-19 | 2012-10-25 | Verinata Health, Inc. | Method for sample analysis of aneuploidies in maternal samples |
| AU2013203624B2 (en) | 2010-02-09 | 2015-06-25 | General Electric Company | Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers |
| US9366632B2 (en) | 2010-02-12 | 2016-06-14 | Raindance Technologies, Inc. | Digital analyte analysis |
| EP2556170A4 (en) | 2010-03-25 | 2014-01-01 | Quantalife Inc | TRAPPING TRANSPORT AND DETECTION SYSTEM |
| JP5573335B2 (ja) | 2010-04-28 | 2014-08-20 | 株式会社島津製作所 | 磁性体粒子操作デバイス及び磁性体粒子操作方法 |
| WO2011154042A1 (en) | 2010-06-10 | 2011-12-15 | Albert-Ludwigs-Universität Freiburg | Apparatus and method for dispensing cells or particles confined in a free flying droplet |
| US9650629B2 (en) | 2010-07-07 | 2017-05-16 | Roche Molecular Systems, Inc. | Clonal pre-amplification in emulsion |
| CN103153466B (zh) | 2010-07-22 | 2016-04-13 | 基因细胞生物系统有限公司 | 复合液体池 |
| US9695390B2 (en) | 2010-08-23 | 2017-07-04 | President And Fellows Of Harvard College | Acoustic waves in microfluidics |
| EP2622103B2 (en) | 2010-09-30 | 2022-11-16 | Bio-Rad Laboratories, Inc. | Sandwich assays in droplets |
| EP2436444A1 (en) | 2010-10-01 | 2012-04-04 | Centre National de la Recherche Scientifique (C.N.R.S.) | Microfluidic device for production and collection of droplets of a fluid |
| DK2625320T3 (da) | 2010-10-08 | 2019-07-01 | Harvard College | High-throughput enkeltcellestregkodning |
| US20120258881A1 (en) | 2010-11-22 | 2012-10-11 | The University Of Chicago | Methods and/or Use of Oligonucleotide Conjugates for Assays and Microscopy/Imaging Detections |
| DK2652155T3 (en) | 2010-12-16 | 2017-02-13 | Gigagen Inc | Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells |
| US20130295587A1 (en) | 2011-01-24 | 2013-11-07 | Ge Healthcare Bio-Sciences Ab | Method of coupling binding agents to a substrate surface |
| US8765455B2 (en) | 2011-01-27 | 2014-07-01 | Lawrence Livermore National Security, Llc | Chip-based droplet sorting |
| EP2670863B1 (en) | 2011-01-31 | 2018-06-27 | H. Hoffnabb-La Roche Ag | Methods of identifying multiple epitopes in cells |
| WO2012106546A2 (en) | 2011-02-02 | 2012-08-09 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
| WO2012109600A2 (en) | 2011-02-11 | 2012-08-16 | Raindance Technologies, Inc. | Methods for forming mixed droplets |
| US9150852B2 (en) | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
| DK3246416T3 (da) | 2011-04-15 | 2024-09-02 | Univ Johns Hopkins | Sikkert sekventeringssystem |
| AU2012249759A1 (en) | 2011-04-25 | 2013-11-07 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| SG194745A1 (en) | 2011-05-20 | 2013-12-30 | Fluidigm Corp | Nucleic acid encoding reactions |
| WO2012162296A2 (en) | 2011-05-23 | 2012-11-29 | President And Fellows Of Harvard College | Control of emulsions, including multiple emulsions |
| US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
| US20130189700A1 (en) | 2011-07-25 | 2013-07-25 | Bio-Rad Laboratories, Inc. | Breakage of an emulsion containing nucleic acid |
| GB2485635B (en) | 2011-07-26 | 2012-11-28 | Verinata Health Inc | Method for determining the presence or absence of different aneuploidies in a sample |
| US20130032235A1 (en) | 2011-08-02 | 2013-02-07 | Teledyne Dalsa Semiconductor, Inc. | Integrated microfluidic check valve and device including such a check valve |
| US9469874B2 (en) | 2011-10-18 | 2016-10-18 | The Regents Of The University Of California | Long-range barcode labeling-sequencing |
| US9483374B2 (en) | 2011-12-21 | 2016-11-01 | Intel Corporation | PSMI using at-speed scan capture |
| US9475013B2 (en) | 2012-02-08 | 2016-10-25 | President And Fellows Of Harvard College | Droplet formation using fluid breakup |
| EP3309262B1 (en) | 2012-02-24 | 2019-09-25 | Bio-Rad Laboratories, Inc. | Labeling and sample preparation for sequencing |
| US11177020B2 (en) | 2012-02-27 | 2021-11-16 | The University Of North Carolina At Chapel Hill | Methods and uses for molecular tags |
| EP3305918B1 (en) | 2012-03-05 | 2020-06-03 | President and Fellows of Harvard College | Methods for epigenetic sequencing |
| PT2850211T (pt) | 2012-05-14 | 2021-11-29 | Irepertoire Inc | Método para aumentar a precisão na deteção quantitativa de polinucleótidos |
| US9223317B2 (en) | 2012-06-14 | 2015-12-29 | Advanced Liquid Logic, Inc. | Droplet actuators that include molecular barrier coatings |
| GB2539836B (en) | 2012-08-13 | 2017-03-29 | Univ California | Methods for detecting target nucleic acids in sample lysate droplets |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US20140378345A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US20140378349A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| WO2014039912A1 (en) | 2012-09-07 | 2014-03-13 | Bio-Rad Laboratories, Inc. | Compositions, systems and methods for droplet formation, spacing and detection |
| EP2898071A4 (en) | 2012-09-21 | 2016-07-20 | Broad Inst Inc | COMPOSITIONS AND METHODS FOR LONG-TERM LABORATORIES AND PREPARED END OF NUCLEIC ACIDS IN EMULSION DROPS |
| AU2013350823B2 (en) | 2012-11-27 | 2017-11-16 | Gencell Biosystems Ltd. | Handling liquid samples |
| EP3567116A1 (en) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| WO2014108323A1 (en) | 2013-01-10 | 2014-07-17 | Eth Zurich | Method and apparatus for depositing droplets onto a substrate |
| CN105324489A (zh) | 2013-03-04 | 2016-02-10 | 韦拉克斯生物医学股份有限公司 | 多分析物试验 |
| US9752179B2 (en) | 2013-03-14 | 2017-09-05 | Cold Spring Harbor Laboratory | Trans-splicing transcriptome profiling |
| WO2014153071A1 (en) | 2013-03-14 | 2014-09-25 | The Broad Institute, Inc. | Methods for quantitating dna using digital multiple displacement amplification |
| US9328382B2 (en) | 2013-03-15 | 2016-05-03 | Complete Genomics, Inc. | Multiple tagging of individual long DNA fragments |
| US10071359B2 (en) | 2013-03-15 | 2018-09-11 | The Regents Of The University Of California | High-speed on demand microfluidic droplet generation and manipulation |
| CN110964796B (zh) | 2013-08-28 | 2024-04-05 | 贝克顿迪金森公司 | 大规模平行单细胞分析 |
| US10947587B2 (en) | 2013-11-05 | 2021-03-16 | The Regents Of The University Of California | Single-cell forensic short tandem repeat typing within microfluidic droplets |
| EP3105326A4 (en) | 2014-02-10 | 2018-01-10 | Gencell Biosystems Limited | Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same |
| CN113215221A (zh) | 2014-04-08 | 2021-08-06 | 华盛顿大学商业中心 | 用于使用多分散小滴执行数字检定的方法和设备 |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| CN106574925B (zh) | 2014-05-23 | 2019-07-26 | 萤火虫生物股份有限公司 | 用于生物测定的底物介导的反应器 |
| WO2015189336A1 (en) | 2014-06-11 | 2015-12-17 | Samplix S.A.R.L. | Nucleotide sequence exclusion enrichment by droplet sorting (needls) |
| CN113249435B (zh) | 2014-06-26 | 2024-09-03 | 10X基因组学有限公司 | 分析来自单个细胞或细胞群体的核酸的方法 |
| EP3160654A4 (en) | 2014-06-27 | 2017-11-15 | The Regents of The University of California | Pcr-activated sorting (pas) |
| WO2016064755A2 (en) | 2014-10-20 | 2016-04-28 | The Regents Of The University Of Califronia | Rapid modulation of droplet composition with membrane microvalves |
| US10434507B2 (en) | 2014-10-22 | 2019-10-08 | The Regents Of The University Of California | High definition microdroplet printer |
| EP3253910B1 (en) | 2015-02-04 | 2021-07-14 | The Regents of The University of California | Multiple-emulsion nucleic acid amplification |
| US11111519B2 (en) | 2015-02-04 | 2021-09-07 | The Regents Of The University Of California | Sequencing of nucleic acids via barcoding in discrete entities |
| EP3337907A4 (en) | 2015-08-17 | 2019-01-02 | The Regents of The University of California | Microdroplet-based multiple displacement amplification (mda) methods and related compositions |
| JP2019515674A (ja) | 2016-04-19 | 2019-06-13 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 遺伝的解析および他の適用のための固定化ベースのシステムおよび方法 |
| US11142791B2 (en) | 2016-08-10 | 2021-10-12 | The Regents Of The University Of California | Combined multiple-displacement amplification and PCR in an emulsion microdroplet |
| WO2018119301A1 (en) | 2016-12-21 | 2018-06-28 | The Regents Of The University Of California | Single cell genomic sequencing using hydrogel based droplets |
| WO2019099908A1 (en) | 2017-11-17 | 2019-05-23 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
-
2013
- 2013-08-12 GB GB1616219.0A patent/GB2539836B/en active Active
- 2013-08-12 EP EP21189969.5A patent/EP4001426A1/en active Pending
- 2013-08-12 GB GB1503594.2A patent/GB2519906B/en active Active
- 2013-08-12 JP JP2015527512A patent/JP6514105B2/ja active Active
- 2013-08-12 US US14/420,646 patent/US10161007B2/en active Active
- 2013-08-12 CN CN201911041486.2A patent/CN110643681A/zh active Pending
- 2013-08-12 AU AU2013302867A patent/AU2013302867A1/en not_active Abandoned
- 2013-08-12 CN CN201380053258.1A patent/CN104736725A/zh active Pending
- 2013-08-12 CA CA2881783A patent/CA2881783A1/en active Pending
- 2013-08-12 EP EP13829925.0A patent/EP2882872B1/en active Active
- 2013-08-12 WO PCT/US2013/054517 patent/WO2014028378A2/en not_active Ceased
-
2016
- 2016-02-18 US US15/047,555 patent/US20160177375A1/en not_active Abandoned
-
2018
- 2018-10-18 US US16/164,707 patent/US11203787B2/en active Active
-
2019
- 2019-02-19 US US16/279,905 patent/US10745762B2/en active Active
- 2019-04-11 US US16/382,080 patent/US11001896B2/en active Active
- 2019-09-06 AU AU2019226236A patent/AU2019226236B2/en active Active
-
2021
- 2021-04-06 US US17/223,565 patent/US11891666B2/en active Active
-
2022
- 2022-06-02 AU AU2022203791A patent/AU2022203791B2/en active Active
-
2023
- 2023-12-18 US US18/544,232 patent/US20240175091A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2015533079A5 (enExample) | ||
| Özay et al. | A review of reaction enhancement strategies for isothermal nucleic acid amplification reactions | |
| US11898142B2 (en) | Multi-effector CRISPR based diagnostic systems | |
| Lobato et al. | Recombinase polymerase amplification: Basics, applications and recent advances | |
| Petralia et al. | In-Check system: A highly integrated silicon Lab-on-Chip for sample preparation, PCR amplification and microarray detection of nucleic acids directly from biological samples | |
| CA3056411A1 (en) | Crispr effector system based diagnostics for virus detection | |
| Santiago-Felipe et al. | Isothermal DNA amplification strategies for duplex microorganism detection | |
| Kim et al. | ChIP–quantitative polymerase chain reaction (ChIP-qPCR) | |
| WO2018172726A1 (en) | Single cell dna sequencing | |
| CN111372574A (zh) | 单分散乳液的制备方法 | |
| JP2018046848A5 (enExample) | ||
| WO2011140489A3 (en) | Integrated sample preparation systems and stabilized enzyme mixtures | |
| JP2015156872A5 (enExample) | ||
| Okochi et al. | Droplet-based gene expression analysis using a device with magnetic force-based-droplet-handling system | |
| US10876106B2 (en) | Gene analysis system | |
| CA3049961A1 (en) | Crispr effector system based diagnostics | |
| Ma et al. | Microfluidics for genome-wide studies involving next generation sequencing | |
| US11118206B2 (en) | Multiple stage isothermal enzymatic amplification | |
| WO2017152122A3 (en) | Automated nested recombinase polymerase amplification | |
| JP2015533507A5 (enExample) | ||
| DK201000356A (en) | New nucleotide sequence amplification Method | |
| EP4296374A3 (en) | Detection of infectious agents from environmental air dust | |
| JP2013543370A5 (enExample) | ||
| CN114829627A (zh) | 基于数字微流控平台富集核酸及构建测序文库的方法 | |
| JP2018520706A5 (enExample) |