JP2015533079A5 - - Google Patents
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- JP2015533079A5 JP2015533079A5 JP2015527512A JP2015527512A JP2015533079A5 JP 2015533079 A5 JP2015533079 A5 JP 2015533079A5 JP 2015527512 A JP2015527512 A JP 2015527512A JP 2015527512 A JP2015527512 A JP 2015527512A JP 2015533079 A5 JP2015533079 A5 JP 2015533079A5
- Authority
- JP
- Japan
- Prior art keywords
- lysate
- droplet
- reagent
- nucleic acid
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims 34
- 239000006166 lysate Substances 0.000 claims 26
- 239000003153 chemical reaction reagent Substances 0.000 claims 20
- 108091033319 polynucleotide Proteins 0.000 claims 20
- 102000040430 polynucleotide Human genes 0.000 claims 20
- 239000002157 polynucleotide Substances 0.000 claims 20
- 230000003321 amplification Effects 0.000 claims 18
- 238000003199 nucleic acid amplification method Methods 0.000 claims 18
- 102000004190 Enzymes Human genes 0.000 claims 15
- 108090000790 Enzymes Proteins 0.000 claims 15
- 238000006243 chemical reaction Methods 0.000 claims 14
- 238000001668 nucleic acid synthesis Methods 0.000 claims 14
- 108091005804 Peptidases Proteins 0.000 claims 12
- 239000004365 Protease Substances 0.000 claims 12
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 12
- 235000019419 proteases Nutrition 0.000 claims 12
- 230000000694 effects Effects 0.000 claims 11
- 239000000047 product Substances 0.000 claims 8
- 230000002194 synthesizing effect Effects 0.000 claims 5
- 239000007795 chemical reaction product Substances 0.000 claims 4
- 239000002612 dispersion medium Substances 0.000 claims 4
- 239000012530 fluid Substances 0.000 claims 4
- 230000000415 inactivating effect Effects 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 239000000758 substrate Substances 0.000 claims 4
- 108010067770 Endopeptidase K Proteins 0.000 claims 3
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 239000013592 cell lysate Substances 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 230000002934 lysing effect Effects 0.000 claims 2
- 230000009089 cytolysis Effects 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 238000010839 reverse transcription Methods 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261682707P | 2012-08-13 | 2012-08-13 | |
| US61/682,707 | 2012-08-13 | ||
| US201361784754P | 2013-03-14 | 2013-03-14 | |
| US61/784,754 | 2013-03-14 | ||
| PCT/US2013/054517 WO2014028378A2 (en) | 2012-08-13 | 2013-08-12 | Methods and systems for detecting biological components |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015533079A JP2015533079A (ja) | 2015-11-19 |
| JP2015533079A5 true JP2015533079A5 (enExample) | 2016-10-27 |
| JP6514105B2 JP6514105B2 (ja) | 2019-05-15 |
Family
ID=50101583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015527512A Active JP6514105B2 (ja) | 2012-08-13 | 2013-08-12 | 生物学的成分を検出するための方法およびシステム |
Country Status (8)
| Country | Link |
|---|---|
| US (7) | US10161007B2 (enExample) |
| EP (2) | EP4001426A1 (enExample) |
| JP (1) | JP6514105B2 (enExample) |
| CN (2) | CN104736725A (enExample) |
| AU (3) | AU2013302867A1 (enExample) |
| CA (1) | CA2881783A1 (enExample) |
| GB (2) | GB2519906B (enExample) |
| WO (1) | WO2014028378A2 (enExample) |
Families Citing this family (178)
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| HK1259411A1 (zh) | 2015-08-17 | 2019-11-29 | 加利福尼亚大学董事会 | 基於微滴的多重置换扩增(mda)方法及相关组合物 |
| US20190127789A1 (en) | 2016-04-19 | 2019-05-02 | President And Fellows Of Harvard College | Immobilization-based systems and methods for genetic analysis and other applications |
| EP3497228A4 (en) | 2016-08-10 | 2020-05-27 | The Regents of The University of California | MULTI-DISPLACEMENT AMPLIFICATION AND PCR COMBINED IN AN EMULSION MICRO DROPLET |
| AU2017382905A1 (en) | 2016-12-21 | 2019-07-04 | The Regents Of The University Of California | Single cell genomic sequencing using hydrogel based droplets |
| EP3559272B1 (en) | 2017-11-17 | 2022-08-31 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
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2013
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