JP2015524281A5 - - Google Patents
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- JP2015524281A5 JP2015524281A5 JP2015526598A JP2015526598A JP2015524281A5 JP 2015524281 A5 JP2015524281 A5 JP 2015524281A5 JP 2015526598 A JP2015526598 A JP 2015526598A JP 2015526598 A JP2015526598 A JP 2015526598A JP 2015524281 A5 JP2015524281 A5 JP 2015524281A5
- Authority
- JP
- Japan
- Prior art keywords
- host cell
- gene
- inducible promoter
- gene product
- cell according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108090000623 proteins and genes Proteins 0.000 claims description 55
- 230000001939 inductive effect Effects 0.000 claims description 29
- 108091033319 polynucleotide Proteins 0.000 claims description 17
- 239000002157 polynucleotide Substances 0.000 claims description 17
- 102000040430 polynucleotide Human genes 0.000 claims description 17
- 229920001184 polypeptide Polymers 0.000 claims description 15
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 15
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 15
- 239000000411 inducer Substances 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims 1
- 239000008101 lactose Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 66
- 238000000034 method Methods 0.000 description 6
- 239000013612 plasmid Substances 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 description 4
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 description 4
- 102000013463 Immunoglobulin Light Chains Human genes 0.000 description 4
- 108010065825 Immunoglobulin Light Chains Proteins 0.000 description 4
- 108010076504 Protein Sorting Signals Proteins 0.000 description 4
- 230000002103 transcriptional effect Effects 0.000 description 4
- 239000012634 fragment Substances 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 101100345719 Bacillus subtilis (strain 168) mmgE gene Proteins 0.000 description 2
- 101100345721 Bacillus subtilis (strain 168) mmgF gene Proteins 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 2
- 108010078791 Carrier Proteins Proteins 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 101100532764 Escherichia coli (strain K12) scpC gene Proteins 0.000 description 2
- 102000004195 Isomerases Human genes 0.000 description 2
- 108090000769 Isomerases Proteins 0.000 description 2
- 108010059896 Manganese peroxidase Proteins 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- 102000006010 Protein Disulfide-Isomerase Human genes 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 101150077538 gor gene Proteins 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 101150079081 pphB gene Proteins 0.000 description 2
- 108020003519 protein disulfide isomerase Proteins 0.000 description 2
- 101150085802 prpB gene Proteins 0.000 description 2
- 101150077061 prpD gene Proteins 0.000 description 2
- 101150057627 trxB gene Proteins 0.000 description 2
- NYHBQMYGNKIUIF-FJFJXFQQSA-N 9-beta-D-arabinofuranosylguanine Chemical compound C12=NC(N)=NC(O)=C2N=CN1[C@@H]1O[C@H](CO)[C@@H](O)[C@@H]1O NYHBQMYGNKIUIF-FJFJXFQQSA-N 0.000 description 1
- 101150019464 ARAF gene Proteins 0.000 description 1
- 101100429087 Bacillus subtilis (strain 168) xlyA gene Proteins 0.000 description 1
- HMFHBZSHGGEWLO-IOVATXLUSA-N D-xylofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@H]1O HMFHBZSHGGEWLO-IOVATXLUSA-N 0.000 description 1
- 101100002294 Escherichia coli (strain K12) argK gene Proteins 0.000 description 1
- 101100095178 Escherichia coli (strain K12) scpB gene Proteins 0.000 description 1
- 125000003599 L-arabinosyl group Chemical group C1([C@H](O)[C@@H](O)[C@@H](O)CO1)* 0.000 description 1
- 108010006519 Molecular Chaperones Proteins 0.000 description 1
- 108020005091 Replication Origin Proteins 0.000 description 1
- 101150088541 SCPA gene Proteins 0.000 description 1
- 101100157012 Thermoanaerobacterium saccharolyticum (strain DSM 8691 / JW/SL-YS485) xynB gene Proteins 0.000 description 1
- 108030002454 Versatile peroxidases Proteins 0.000 description 1
- OIRDTQYFTABQOQ-UHTZMRCNSA-N Vidarabine Chemical group C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@@H]1O OIRDTQYFTABQOQ-UHTZMRCNSA-N 0.000 description 1
- 108700040099 Xylose isomerases Proteins 0.000 description 1
- OIRDTQYFTABQOQ-UHFFFAOYSA-N ara-adenosine Natural products Nc1ncnc2n(cnc12)C1OC(CO)C(O)C1O OIRDTQYFTABQOQ-UHFFFAOYSA-N 0.000 description 1
- 101150035354 araA gene Proteins 0.000 description 1
- 101150097746 araB gene Proteins 0.000 description 1
- 101150017736 araD gene Proteins 0.000 description 1
- 101150076178 araE gene Proteins 0.000 description 1
- 101150084021 araG gene Proteins 0.000 description 1
- 101150023525 araH gene Proteins 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 101150092394 argK gene Proteins 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 150000002019 disulfides Chemical class 0.000 description 1
- 101150096947 gshB gene Proteins 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 101150091078 rhaA gene Proteins 0.000 description 1
- 101150032455 rhaB gene Proteins 0.000 description 1
- 101150052254 rhaD gene Proteins 0.000 description 1
- 101150097837 rhaT gene Proteins 0.000 description 1
- 101150024466 scpB gene Proteins 0.000 description 1
- 101150033155 sspP gene Proteins 0.000 description 1
- 101150011516 xlnD gene Proteins 0.000 description 1
- 101150052264 xylA gene Proteins 0.000 description 1
- 101150110790 xylB gene Proteins 0.000 description 1
- 101150010108 xylF gene Proteins 0.000 description 1
- 101150068654 xylG gene Proteins 0.000 description 1
- 101150004248 xylH gene Proteins 0.000 description 1
- 101150068563 ygfI gene Proteins 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261679751P | 2012-08-05 | 2012-08-05 | |
| US61/679,751 | 2012-08-05 | ||
| US201261747246P | 2012-12-29 | 2012-12-29 | |
| US61/747,246 | 2012-12-29 | ||
| PCT/US2013/053562 WO2014025663A1 (en) | 2012-08-05 | 2013-08-05 | Inducible coexpression system |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018237995A Division JP7032292B2 (ja) | 2012-08-05 | 2018-12-20 | 誘導性共発現系 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015524281A JP2015524281A (ja) | 2015-08-24 |
| JP2015524281A5 true JP2015524281A5 (https=) | 2016-09-29 |
| JP6457387B2 JP6457387B2 (ja) | 2019-01-23 |
Family
ID=49111529
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015526598A Active JP6457387B2 (ja) | 2012-08-05 | 2013-08-05 | 誘導性共発現系 |
| JP2018237995A Active JP7032292B2 (ja) | 2012-08-05 | 2018-12-20 | 誘導性共発現系 |
| JP2022026494A Pending JP2022068332A (ja) | 2012-08-05 | 2022-02-24 | 誘導性共発現系 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018237995A Active JP7032292B2 (ja) | 2012-08-05 | 2018-12-20 | 誘導性共発現系 |
| JP2022026494A Pending JP2022068332A (ja) | 2012-08-05 | 2022-02-24 | 誘導性共発現系 |
Country Status (11)
| Country | Link |
|---|---|
| US (8) | US9617335B2 (https=) |
| EP (4) | EP2880168B1 (https=) |
| JP (3) | JP6457387B2 (https=) |
| CN (2) | CN111748510B (https=) |
| AU (3) | AU2013299910B2 (https=) |
| CA (1) | CA2880285C (https=) |
| DK (2) | DK3187586T3 (https=) |
| ES (3) | ES2954516T3 (https=) |
| IL (3) | IL236975B (https=) |
| PL (1) | PL3591057T3 (https=) |
| WO (1) | WO2014025663A1 (https=) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112014029417B1 (pt) * | 2012-05-25 | 2023-03-07 | Cellectis | Método ex vivo para a preparação de células t para imunoterapia |
| US20150353940A1 (en) * | 2013-08-05 | 2015-12-10 | Absci, Llc | Vectors for use in an inducible coexpression system |
| US12157766B2 (en) * | 2012-08-05 | 2024-12-03 | Absci Corporation | Cytoplasmic expression system |
| US11077144B2 (en) | 2013-05-13 | 2021-08-03 | Cellectis | CD19 specific chimeric antigen receptor and uses thereof |
| JP6788255B2 (ja) * | 2015-11-19 | 2020-11-25 | 国立大学法人信州大学 | 新規ベクター及びこれを用いた可溶化タンパク質の製造方法 |
| SG11201811478TA (en) * | 2016-01-12 | 2019-01-30 | Intron Biotechnology Inc | Freeze-dried formulations of antibacterial protein |
| CN106190940B (zh) * | 2016-07-19 | 2019-06-14 | 北京三元基因药业股份有限公司 | 表达抗TNF抗体Fab片段的重组大肠杆菌工程菌 |
| KR102480965B1 (ko) | 2016-09-13 | 2022-12-26 | 알레간 인코포레이티드 | 안정화된 비단백질 클로스트리듐 독소 조성물 |
| WO2019175881A1 (en) * | 2018-03-14 | 2019-09-19 | Technion Research & Development Foundation Limited | A prognostic biomarker in cancer |
| JP7174990B2 (ja) * | 2018-07-23 | 2022-11-18 | 国立大学法人山口大学 | 原核生物用発現ベクター |
| WO2020069011A1 (en) | 2018-09-25 | 2020-04-02 | Absci, Llc | Protein purification methods |
| ES2944837T3 (es) | 2018-11-08 | 2023-06-26 | Sutro Biopharma Inc | Cepas de E. coli con citoplasma oxidativo |
| CN109371049A (zh) * | 2018-11-14 | 2019-02-22 | 天津大学 | 分子伴侣在促进单克隆抗体的形成和/或提高单克隆抗体的表达量中的应用 |
| CN111269294B (zh) * | 2018-12-05 | 2023-03-28 | 广州中国科学院先进技术研究所 | 纤维寡糖转运蛋白LacY的突变位点、突变转运蛋白LacY及其制备方法和应用 |
| EP3935154A1 (en) * | 2019-04-04 | 2022-01-12 | Braskem S.A. | Metabolic engineering for simultaneous consumption of xylose and glucose for production of chemicals from second generation sugars |
| CN110616181A (zh) * | 2019-09-27 | 2019-12-27 | 北京理工大学 | 一株基于大肠杆菌改造的工程菌株 |
| CN112980891B (zh) * | 2019-12-16 | 2023-12-12 | 中国科学院分子植物科学卓越创新中心 | 一种基于CRISPR-Cas的大肠杆菌基因组编辑工具 |
| US12351850B2 (en) | 2020-04-30 | 2025-07-08 | Sutro Biopharma, Inc. | Methods of producing full-length antibodies using E. coli |
| BE1029145B1 (fr) * | 2021-02-26 | 2022-09-27 | Curavac Europe | Methode de production d'une forme periplasmique de la proteine crm197 |
| CN113122552B (zh) * | 2021-04-21 | 2022-07-26 | 西北农林科技大学 | 黄瓜谷氧还蛋白基因CsGRX4及其灰霉菌感病性的应用 |
| JP2024533596A (ja) | 2021-09-17 | 2024-09-12 | アブサイ コーポレイション | トランスファーｒｎａの組成、及び非標準アミノ酸を含むタンパク質の生産における使用 |
| US20250383346A1 (en) * | 2022-06-29 | 2025-12-18 | Absci Corporation | Affinity screening method |
| WO2024040020A1 (en) | 2022-08-15 | 2024-02-22 | Absci Corporation | Quantitative affinity activity specific cell enrichment |
| CN120731217A (zh) | 2022-12-21 | 2025-09-30 | 唐纳德丹福斯植物科学中心 | 抗微生物肽及其修饰 |
| CN116875524B (zh) * | 2023-09-08 | 2024-03-26 | 清华大学 | 一种在微生物中调控多基因表达的方法及其应用 |
| WO2025058962A1 (en) | 2023-09-11 | 2025-03-20 | Absci Corporation | High-throughput methods for kinetic characterization, quantifying and optimizing antibodies and antibody fragments expression in bacteria |
| US20250109209A1 (en) | 2023-10-03 | 2025-04-03 | Absci Corporation | Tl1a associated antibody compositions and methods of use |
| WO2025122885A1 (en) | 2023-12-08 | 2025-06-12 | Absci Corporation | Anti-her2 associated antibody compositions designed by artificial intelligence and methods of use |
| WO2025144700A1 (en) | 2023-12-27 | 2025-07-03 | Absci Corporation | Nanobody library screening using bacterial surface display |
| CN118064437B (zh) * | 2024-04-24 | 2024-09-10 | 山东福洋生物科技股份有限公司 | 一种多启动子结构元件及其构建方法和应用 |
| WO2025259864A1 (en) | 2024-06-12 | 2025-12-18 | Absci Corporation | Expression of proteins containing non-standard amino acids |
| CN119410683B (zh) * | 2025-01-08 | 2025-05-23 | 北京化工大学 | 一种生物发酵-全细胞催化级联生产己二酸的方法 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2660397A (en) * | 1996-04-05 | 1997-10-29 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells |
| US6083715A (en) | 1997-06-09 | 2000-07-04 | Board Of Regents, The University Of Texas System | Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells |
| CN1064052C (zh) | 1997-09-29 | 2001-04-04 | 中国科学院微生物研究所 | 甲状旁腺素相关蛋白人源化抗体及其制备方法 |
| US6872563B1 (en) * | 1999-10-05 | 2005-03-29 | President And Fellows Of Harvard College | Compositions and methods for production of disulfide bond containing proteins in host cells |
| AU2001249265B2 (en) | 2000-03-24 | 2006-10-26 | Xoma Technology Ltd. | Methods and cells for expression of recombinant protein products |
| US6979556B2 (en) * | 2000-12-14 | 2005-12-27 | Genentech, Inc. | Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes |
| CN100513414C (zh) * | 2001-08-27 | 2009-07-15 | 杰南技术公司 | 进行抗体表达和装配的系统 |
| ATE534669T1 (de) * | 2002-05-28 | 2011-12-15 | Omrix Biopharmaceuticals Inc | Verfahren zum erhalten von anti-idiotyp antikörpern |
| EP1539794B1 (en) * | 2002-07-03 | 2008-12-31 | Dow Global Technologies Inc. | Benzoate- and anthranilate-inducible promoters |
| PT1570060E (pt) * | 2002-12-02 | 2007-09-13 | Basf Ag | ''sistema de expressão indutível de l-ramnose'' |
| JP5176114B2 (ja) * | 2005-06-06 | 2013-04-03 | フェネックス インコーポレイテッド | 細菌宿主細胞におけるマンニトール誘導のプロモーター系 |
| US7351563B2 (en) | 2005-06-10 | 2008-04-01 | The Board Of Trustees Of The Leland Stanford Junior University | Cell-free extracts and synthesis of active hydrogenase |
| US8178338B2 (en) | 2005-07-01 | 2012-05-15 | The Regents Of The University Of California | Inducible expression vectors and methods of use thereof |
| JP4270466B2 (ja) * | 2005-09-02 | 2009-06-03 | 国立大学法人福井大学 | ＮＡＤａｓｅ、ＳＮＩおよびＳＬＯ遺伝子を含むオペロンから発現するタンパク質の製造方法、それにより得られるタンパク質およびその使用 |
| US7816117B2 (en) | 2006-04-26 | 2010-10-19 | President And Fellows Of Harvard College | Prokaryotic host cells for expressing proteins rich in disulfide bonds |
| EP2155868A2 (en) * | 2007-04-19 | 2010-02-24 | Codon Devices, Inc | Engineered nucleases and their uses for nucleic acid assembly |
| EP2155789B1 (en) | 2007-05-01 | 2013-07-24 | Research Development Foundation | Immunoglobulin fc libraries |
| CN107119095B (zh) | 2008-01-03 | 2022-07-05 | 康乃尔研究基金会有限公司 | 原核生物中的糖基化蛋白表达 |
| FI20095615A0 (fi) * | 2009-06-02 | 2009-06-02 | Oulun Yliopisto | Menetelmä luonnollisesti laskostuneiden proteiinien tuottamiseksi prokaryootti-isännässä |
| WO2011116184A2 (en) * | 2010-03-17 | 2011-09-22 | Cornell University | Methods and compositions for targeted mutagenesis in bacteria |
| EP2386641A1 (en) * | 2010-05-11 | 2011-11-16 | Université Catholique De Louvain | Method for extracellular production of recombinant biomolecules in a microorganism |
| FI20115115A0 (fi) | 2011-02-04 | 2011-02-04 | Oulun Yliopisto | Menetelmä disulfidisidoksia sisältävien proteiinien tuottamiseksi prokaryoottisytoplasmassa |
-
2013
- 2013-08-05 DK DK16201998.8T patent/DK3187586T3/da active
- 2013-08-05 CN CN202010672668.6A patent/CN111748510B/zh active Active
- 2013-08-05 PL PL19187102.9T patent/PL3591057T3/pl unknown
- 2013-08-05 ES ES19187102T patent/ES2954516T3/es active Active
- 2013-08-05 EP EP13756743.4A patent/EP2880168B1/en active Active
- 2013-08-05 CA CA2880285A patent/CA2880285C/en active Active
- 2013-08-05 ES ES16201998T patent/ES2750005T3/es active Active
- 2013-08-05 EP EP23176713.8A patent/EP4273257A3/en active Pending
- 2013-08-05 US US14/419,653 patent/US9617335B2/en active Active
- 2013-08-05 EP EP19187102.9A patent/EP3591057B9/en active Active
- 2013-08-05 JP JP2015526598A patent/JP6457387B2/ja active Active
- 2013-08-05 ES ES13756743.4T patent/ES2621320T3/es active Active
- 2013-08-05 DK DK13756743.4T patent/DK2880168T3/en active
- 2013-08-05 EP EP16201998.8A patent/EP3187586B1/en active Active
- 2013-08-05 CN CN201380041522.XA patent/CN104603274B/zh active Active
- 2013-08-05 WO PCT/US2013/053562 patent/WO2014025663A1/en not_active Ceased
- 2013-08-05 AU AU2013299910A patent/AU2013299910B2/en active Active
-
2015
- 2015-01-29 IL IL236975A patent/IL236975B/en active IP Right Grant
- 2015-11-25 US US14/952,535 patent/US10465197B2/en active Active
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2017
- 2017-02-16 US US15/434,869 patent/US10604761B2/en active Active
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2018
- 2018-12-20 JP JP2018237995A patent/JP7032292B2/ja active Active
-
2019
- 2019-01-21 IL IL264384A patent/IL264384B/en active IP Right Grant
- 2019-04-23 AU AU2019202844A patent/AU2019202844B2/en active Active
- 2019-10-07 US US16/595,371 patent/US11214807B2/en active Active
- 2019-11-05 US US16/675,102 patent/US20200063116A1/en not_active Abandoned
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2020
- 2020-02-13 US US16/790,430 patent/US11447781B2/en active Active
- 2020-07-19 IL IL276141A patent/IL276141B/en unknown
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2021
- 2021-12-06 US US17/542,698 patent/US20220090093A1/en not_active Abandoned
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2022
- 2022-02-24 JP JP2022026494A patent/JP2022068332A/ja active Pending
- 2022-06-16 AU AU2022204192A patent/AU2022204192A1/en not_active Abandoned
-
2023
- 2023-05-01 US US18/310,317 patent/US20230340501A1/en not_active Abandoned
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