JP2018518957A5 - - Google Patents
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- JP2018518957A5 JP2018518957A5 JP2017565149A JP2017565149A JP2018518957A5 JP 2018518957 A5 JP2018518957 A5 JP 2018518957A5 JP 2017565149 A JP2017565149 A JP 2017565149A JP 2017565149 A JP2017565149 A JP 2017565149A JP 2018518957 A5 JP2018518957 A5 JP 2018518957A5
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- 239000002773 nucleotide Substances 0.000 claims description 101
- 125000003729 nucleotide group Chemical group 0.000 claims description 101
- 108090000623 proteins and genes Proteins 0.000 claims description 95
- 210000004027 cell Anatomy 0.000 claims description 69
- 230000001939 inductive effect Effects 0.000 claims description 64
- 239000000411 inducer Substances 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 18
- 108091033319 polynucleotide Proteins 0.000 claims description 16
- 239000002157 polynucleotide Substances 0.000 claims description 16
- 102000040430 polynucleotide Human genes 0.000 claims description 16
- 102000004169 proteins and genes Human genes 0.000 claims description 16
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 11
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical group CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 claims description 9
- 101150076178 araE gene Proteins 0.000 claims description 8
- 101150085802 prpB gene Proteins 0.000 claims description 7
- 102000014914 Carrier Proteins Human genes 0.000 claims description 6
- 108010078791 Carrier Proteins Proteins 0.000 claims description 6
- 101100532764 Escherichia coli (strain K12) scpC gene Proteins 0.000 claims description 6
- 108010079911 Thioredoxin-disulfide reductase Proteins 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 6
- 230000035772 mutation Effects 0.000 claims description 6
- 101100345719 Bacillus subtilis (strain 168) mmgE gene Proteins 0.000 claims description 5
- 101100345721 Bacillus subtilis (strain 168) mmgF gene Proteins 0.000 claims description 5
- 125000003599 L-arabinosyl group Chemical group C1([C@H](O)[C@@H](O)[C@@H](O)CO1)* 0.000 claims description 5
- OIRDTQYFTABQOQ-UHTZMRCNSA-N Vidarabine Chemical group C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@@H]1O OIRDTQYFTABQOQ-UHTZMRCNSA-N 0.000 claims description 5
- OIRDTQYFTABQOQ-UHFFFAOYSA-N ara-adenosine Natural products Nc1ncnc2n(cnc12)C1OC(CO)C(O)C1O OIRDTQYFTABQOQ-UHFFFAOYSA-N 0.000 claims description 5
- 101150035354 araA gene Proteins 0.000 claims description 5
- 101150079081 pphB gene Proteins 0.000 claims description 5
- 101150077061 prpD gene Proteins 0.000 claims description 5
- 101150057627 trxB gene Proteins 0.000 claims description 5
- 230000002103 transcriptional effect Effects 0.000 claims description 4
- NYHBQMYGNKIUIF-FJFJXFQQSA-N 9-beta-D-arabinofuranosylguanine Chemical compound C12=NC(N)=NC(O)=C2N=CN1[C@@H]1O[C@H](CO)[C@@H](O)[C@@H]1O NYHBQMYGNKIUIF-FJFJXFQQSA-N 0.000 claims description 3
- 101150019464 ARAF gene Proteins 0.000 claims description 3
- HMFHBZSHGGEWLO-IOVATXLUSA-N D-xylofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@H]1O HMFHBZSHGGEWLO-IOVATXLUSA-N 0.000 claims description 3
- 241000588724 Escherichia coli Species 0.000 claims description 3
- 101100002294 Escherichia coli (strain K12) argK gene Proteins 0.000 claims description 3
- 101100416564 Escherichia coli (strain K12) rfbA gene Proteins 0.000 claims description 3
- 101100095178 Escherichia coli (strain K12) scpB gene Proteins 0.000 claims description 3
- 101150088541 SCPA gene Proteins 0.000 claims description 3
- 101100157012 Thermoanaerobacterium saccharolyticum (strain DSM 8691 / JW/SL-YS485) xynB gene Proteins 0.000 claims description 3
- 101150097746 araB gene Proteins 0.000 claims description 3
- 101150084021 araG gene Proteins 0.000 claims description 3
- 101150023525 araH gene Proteins 0.000 claims description 3
- 101150092394 argK gene Proteins 0.000 claims description 3
- 108010066381 preproinsulin Proteins 0.000 claims description 3
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 101150055510 rfbB gene Proteins 0.000 claims description 3
- 101150117748 rfbC gene Proteins 0.000 claims description 3
- 101150061409 rfbD gene Proteins 0.000 claims description 3
- 101150091078 rhaA gene Proteins 0.000 claims description 3
- 101150032455 rhaB gene Proteins 0.000 claims description 3
- 101150052254 rhaD gene Proteins 0.000 claims description 3
- 101150097837 rhaT gene Proteins 0.000 claims description 3
- 101150099889 rmlA gene Proteins 0.000 claims description 3
- 101150043440 rmlB gene Proteins 0.000 claims description 3
- 101150016581 rmlC gene Proteins 0.000 claims description 3
- 101150078780 rmlD gene Proteins 0.000 claims description 3
- 101150024466 scpB gene Proteins 0.000 claims description 3
- 101150033155 sspP gene Proteins 0.000 claims description 3
- 101150011516 xlnD gene Proteins 0.000 claims description 3
- 101150052264 xylA gene Proteins 0.000 claims description 3
- 101150110790 xylB gene Proteins 0.000 claims description 3
- 101150010108 xylF gene Proteins 0.000 claims description 3
- 101150068654 xylG gene Proteins 0.000 claims description 3
- 101150004248 xylH gene Proteins 0.000 claims description 3
- 101100429087 Bacillus subtilis (strain 168) xlyA gene Proteins 0.000 claims description 2
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 claims description 2
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 claims description 2
- 102000013463 Immunoglobulin Light Chains Human genes 0.000 claims description 2
- 108010065825 Immunoglobulin Light Chains Proteins 0.000 claims description 2
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- 101100084022 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) lapA gene Proteins 0.000 claims description 2
- 239000012634 fragment Substances 0.000 claims description 2
- 101150009573 phoA gene Proteins 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims 2
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 claims 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 claims 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 claims 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims 1
- 230000007812 deficiency Effects 0.000 claims 1
- 239000008101 lactose Substances 0.000 claims 1
- SRBFZHDQGSBBOR-HWQSCIPKSA-N L-arabinopyranose Chemical compound O[C@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-HWQSCIPKSA-N 0.000 description 5
- 108010036164 Glutathione synthase Proteins 0.000 description 4
- 101100015982 Dictyostelium discoideum gcsA gene Proteins 0.000 description 2
- 101100172084 Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) egtA gene Proteins 0.000 description 2
- 101100369961 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) erv-1 gene Proteins 0.000 description 2
- 102000006010 Protein Disulfide-Isomerase Human genes 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 101150019860 gshA gene Proteins 0.000 description 2
- 101150096947 gshB gene Proteins 0.000 description 2
- 108020003519 protein disulfide isomerase Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021106797A JP2021153600A (ja) | 2015-06-16 | 2021-06-28 | 誘導性共発現系における使用のためのベクター |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14/740,475 US20150353940A1 (en) | 2013-08-05 | 2015-06-16 | Vectors for use in an inducible coexpression system |
| US14/740,475 | 2015-06-16 | ||
| PCT/US2016/037942 WO2016205570A1 (en) | 2015-06-16 | 2016-06-16 | Vectors for use in an inducible coexpression system |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021106797A Division JP2021153600A (ja) | 2015-06-16 | 2021-06-28 | 誘導性共発現系における使用のためのベクター |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018518957A JP2018518957A (ja) | 2018-07-19 |
| JP2018518957A5 true JP2018518957A5 (https=) | 2019-07-25 |
| JP6976860B2 JP6976860B2 (ja) | 2021-12-08 |
Family
ID=54769087
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017565149A Active JP6976860B2 (ja) | 2015-06-16 | 2016-06-16 | 誘導性共発現系における使用のためのベクター |
| JP2021106797A Pending JP2021153600A (ja) | 2015-06-16 | 2021-06-28 | 誘導性共発現系における使用のためのベクター |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021106797A Pending JP2021153600A (ja) | 2015-06-16 | 2021-06-28 | 誘導性共発現系における使用のためのベクター |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US20150353940A1 (https=) |
| EP (2) | EP3310919B1 (https=) |
| JP (2) | JP6976860B2 (https=) |
| KR (1) | KR102716963B1 (https=) |
| CN (1) | CN107849572A (https=) |
| AU (2) | AU2016278242B2 (https=) |
| CA (1) | CA2986443A1 (https=) |
| DK (1) | DK3310919T3 (https=) |
| ES (1) | ES2906725T3 (https=) |
| IL (2) | IL256094B2 (https=) |
| MX (1) | MX390195B (https=) |
| WO (1) | WO2016205570A1 (https=) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017106583A1 (en) * | 2015-12-15 | 2017-06-22 | Absci, Llc | Cytoplasmic expression system |
| US12157766B2 (en) | 2012-08-05 | 2024-12-03 | Absci Corporation | Cytoplasmic expression system |
| WO2020069011A1 (en) | 2018-09-25 | 2020-04-02 | Absci, Llc | Protein purification methods |
| EP3877407B1 (en) * | 2018-11-05 | 2026-03-11 | F. Hoffmann-La Roche AG | Methods of producing two chain proteins in prokaryotic host cells |
| CN111269294B (zh) * | 2018-12-05 | 2023-03-28 | 广州中国科学院先进技术研究所 | 纤维寡糖转运蛋白LacY的突变位点、突变转运蛋白LacY及其制备方法和应用 |
| CN111979206B (zh) * | 2019-05-24 | 2021-08-17 | 深圳瑞德林生物技术有限公司 | 固定化融合酶及用其制备谷胱甘肽的方法 |
| WO2022020748A1 (en) * | 2020-07-24 | 2022-01-27 | Duke University | Methods and compositions for the production of acetyl-coa derived products |
| US20220380783A1 (en) * | 2021-05-06 | 2022-12-01 | Arizona Board Of Regents On Behalf Of Arizona State University | Methods and system of managing resource competition in synthetic gene circuits |
| CN113528410A (zh) * | 2021-06-23 | 2021-10-22 | 云舟生物科技（广州）有限公司 | 一种用于快速高效诱导表达外源蛋白的大肠杆菌工程菌及其制备方法与应用 |
| WO2023114905A1 (en) | 2021-12-16 | 2023-06-22 | Absci Corporation | Membrane-associated fusion proteins to increase the competency of cells |
| US20250051760A1 (en) | 2021-12-17 | 2025-02-13 | Absci Corporation | Solid-phase screening for high-performing bacterial strains |
| AU2022420494A1 (en) | 2021-12-21 | 2024-07-18 | Absci Corporation | Fola selection assay to identify strains with increased soluble target protein expression |
| EP4453198A1 (en) | 2021-12-23 | 2024-10-30 | ABSCI Corporation | Products and methods for heterologous expression of proteins in a host cell |
| WO2023129881A1 (en) * | 2021-12-30 | 2023-07-06 | Absci Corporation | Knockout of ptsp gene elevates active gene expression |
| US20230268026A1 (en) | 2022-01-07 | 2023-08-24 | Absci Corporation | Designing biomolecule sequence variants with pre-specified attributes |
| US20250383346A1 (en) | 2022-06-29 | 2025-12-18 | Absci Corporation | Affinity screening method |
| WO2024040020A1 (en) | 2022-08-15 | 2024-02-22 | Absci Corporation | Quantitative affinity activity specific cell enrichment |
| WO2024254237A1 (en) | 2023-06-08 | 2024-12-12 | Absci Corporation | Solid-phase screening for high-performing bacterial strains |
| WO2025058962A1 (en) | 2023-09-11 | 2025-03-20 | Absci Corporation | High-throughput methods for kinetic characterization, quantifying and optimizing antibodies and antibody fragments expression in bacteria |
| US20250109209A1 (en) | 2023-10-03 | 2025-04-03 | Absci Corporation | Tl1a associated antibody compositions and methods of use |
| WO2025122885A1 (en) | 2023-12-08 | 2025-06-12 | Absci Corporation | Anti-her2 associated antibody compositions designed by artificial intelligence and methods of use |
| WO2025144700A1 (en) | 2023-12-27 | 2025-07-03 | Absci Corporation | Nanobody library screening using bacterial surface display |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3344618B2 (ja) | 1997-06-20 | 2002-11-11 | 株式会社エイチ・エス・ピー研究所 | シャペロン発現プラスミド |
| US6509156B1 (en) * | 1997-12-05 | 2003-01-21 | Europaisches Laboratorium Fur Molekularoiologie (Embl) | DNA cloning method relying on the E. coli recE/recT recombination system |
| US6780405B1 (en) | 2000-04-28 | 2004-08-24 | Avant Immunotherapeutics, Inc. | Regulated antigen delivery system (RADS) |
| WO2002061090A2 (en) * | 2000-12-14 | 2002-08-08 | Genentech, Inc. | Prokaryotically produced antibodies and uses thereof |
| US6979556B2 (en) * | 2000-12-14 | 2005-12-27 | Genentech, Inc. | Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes |
| CN100513414C (zh) | 2001-08-27 | 2009-07-15 | 杰南技术公司 | 进行抗体表达和装配的系统 |
| WO2005040388A2 (en) * | 2003-08-22 | 2005-05-06 | Nucleonics Inc. | Eukariotic expression systems for expression of inhibitory rna in multiple intracellular compartments |
| US8227241B2 (en) * | 2004-03-12 | 2012-07-24 | Unigene Laboratories, Inc. | Bacterial host cell for the direct expression of peptides |
| DE102004061846A1 (de) * | 2004-12-22 | 2006-07-13 | Basf Ag | Mehrfachpromotoren |
| JP5176114B2 (ja) | 2005-06-06 | 2013-04-03 | フェネックス インコーポレイテッド | 細菌宿主細胞におけるマンニトール誘導のプロモーター系 |
| US8178338B2 (en) * | 2005-07-01 | 2012-05-15 | The Regents Of The University Of California | Inducible expression vectors and methods of use thereof |
| EP2155789B1 (en) | 2007-05-01 | 2013-07-24 | Research Development Foundation | Immunoglobulin fc libraries |
| CN107119095B (zh) | 2008-01-03 | 2022-07-05 | 康乃尔研究基金会有限公司 | 原核生物中的糖基化蛋白表达 |
| KR100958096B1 (ko) * | 2008-02-11 | 2010-05-14 | 한국과학기술원 | 염색체 특정 부위 제거용 재조합 벡터 및 이를 이용한미생물 내 염색체 특정 부위의 제거방법 |
| WO2010075956A1 (de) * | 2008-12-16 | 2010-07-08 | C-Lecta Gmbh | Expressionsvektor |
| AU2010224238A1 (en) * | 2009-03-10 | 2011-09-22 | Athena Biotechnologies, Inc. | Reducing carbon dioxide production and increasing ethanol yield during microbial ethanol fermentation |
| US8623652B2 (en) * | 2009-04-06 | 2014-01-07 | Lucigen Corporation | Host-vector system for cloning and expressing genes |
| NO2501406T3 (https=) * | 2009-11-19 | 2018-05-19 | ||
| WO2011116184A2 (en) * | 2010-03-17 | 2011-09-22 | Cornell University | Methods and compositions for targeted mutagenesis in bacteria |
| EP2386641A1 (en) | 2010-05-11 | 2011-11-16 | Université Catholique De Louvain | Method for extracellular production of recombinant biomolecules in a microorganism |
| DK3187586T3 (da) * | 2012-08-05 | 2019-10-14 | Absci Llc | Inducerbar co-ekspressionssystem |
| EP3030651A4 (en) * | 2013-08-05 | 2017-02-22 | Aronora, Inc. | Coordinated coexpression of thrombin |
-
2015
- 2015-06-16 US US14/740,475 patent/US20150353940A1/en not_active Abandoned
-
2016
- 2016-06-16 EP EP16812476.6A patent/EP3310919B1/en active Active
- 2016-06-16 CN CN201680035423.4A patent/CN107849572A/zh active Pending
- 2016-06-16 DK DK16812476.6T patent/DK3310919T3/da active
- 2016-06-16 WO PCT/US2016/037942 patent/WO2016205570A1/en not_active Ceased
- 2016-06-16 JP JP2017565149A patent/JP6976860B2/ja active Active
- 2016-06-16 AU AU2016278242A patent/AU2016278242B2/en active Active
- 2016-06-16 CA CA2986443A patent/CA2986443A1/en active Pending
- 2016-06-16 KR KR1020187000942A patent/KR102716963B1/ko active Active
- 2016-06-16 MX MX2017016400A patent/MX390195B/es unknown
- 2016-06-16 EP EP21214179.0A patent/EP4039810A1/en active Pending
- 2016-06-16 ES ES16812476T patent/ES2906725T3/es active Active
- 2016-06-16 IL IL256094A patent/IL256094B2/en unknown
- 2016-06-16 IL IL307555A patent/IL307555A/en unknown
- 2016-09-11 US US15/261,984 patent/US11371048B2/en active Active
-
2021
- 2021-06-28 JP JP2021106797A patent/JP2021153600A/ja active Pending
-
2022
- 2022-09-08 AU AU2022228166A patent/AU2022228166A1/en not_active Abandoned
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