EP3030651A4 - Coordinated coexpression of thrombin - Google Patents

Coordinated coexpression of thrombin Download PDF

Info

Publication number
EP3030651A4
EP3030651A4 EP14834543.2A EP14834543A EP3030651A4 EP 3030651 A4 EP3030651 A4 EP 3030651A4 EP 14834543 A EP14834543 A EP 14834543A EP 3030651 A4 EP3030651 A4 EP 3030651A4
Authority
EP
European Patent Office
Prior art keywords
coexpression
thrombin
coordinated
coordinated coexpression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP14834543.2A
Other languages
German (de)
French (fr)
Other versions
EP3030651A1 (en
Inventor
Andras Gruber
Sean Mcclain
Mark VALASEK
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ARONORA Inc
Absci LLC
Original Assignee
ARONORA Inc
Absci LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2013/053562 external-priority patent/WO2014025663A1/en
Application filed by ARONORA Inc, Absci LLC filed Critical ARONORA Inc
Publication of EP3030651A1 publication Critical patent/EP3030651A1/en
Publication of EP3030651A4 publication Critical patent/EP3030651A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6429—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)
EP14834543.2A 2013-08-05 2014-02-05 Coordinated coexpression of thrombin Withdrawn EP3030651A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US2013/053562 WO2014025663A1 (en) 2012-08-05 2013-08-05 Inducible coexpression system
PCT/US2014/014968 WO2015020690A1 (en) 2013-08-05 2014-02-05 Coordinated coexpression of thrombin

Publications (2)

Publication Number Publication Date
EP3030651A1 EP3030651A1 (en) 2016-06-15
EP3030651A4 true EP3030651A4 (en) 2017-02-22

Family

ID=52462067

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14834543.2A Withdrawn EP3030651A4 (en) 2013-08-05 2014-02-05 Coordinated coexpression of thrombin

Country Status (2)

Country Link
EP (1) EP3030651A4 (en)
WO (1) WO2015020690A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150353940A1 (en) * 2013-08-05 2015-12-10 Absci, Llc Vectors for use in an inducible coexpression system
CN115850517A (en) * 2022-11-14 2023-03-28 沈阳药科大学 Method for improving yield of GLP-1Fc fusion protein

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2386641A1 (en) * 2010-05-11 2011-11-16 Université Catholique De Louvain Method for extracellular production of recombinant biomolecules in a microorganism
WO2012088220A2 (en) * 2010-12-22 2012-06-28 Saint Louis University Expression of thrombin variants

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002100337A2 (en) * 2001-06-08 2002-12-19 Emory University Antithrombotic thrombin variants
US8178338B2 (en) * 2005-07-01 2012-05-15 The Regents Of The University Of California Inducible expression vectors and methods of use thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2386641A1 (en) * 2010-05-11 2011-11-16 Université Catholique De Louvain Method for extracellular production of recombinant biomolecules in a microorganism
WO2012088220A2 (en) * 2010-12-22 2012-06-28 Saint Louis University Expression of thrombin variants

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
JULIE LOBSTEIN ET AL: "SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm", MICROBIAL CELL FACTORIES, BIOMED CENTRAL, LONDON, NL, vol. 11, no. 1, 8 May 2012 (2012-05-08), pages 56, XP021129014, ISSN: 1475-2859, DOI: 10.1186/1475-2859-11-56 *
KHLEBNIKOV A ET AL: "Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, GB, vol. 147, no. Pt 12, 1 December 2001 (2001-12-01), pages 3241 - 3247, XP002364204, ISSN: 1350-0872 *
S. K. LEE ET AL: "A Propionate-Inducible Expression System for Enteric Bacteria", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 71, no. 11, 1 November 2005 (2005-11-01), pages 6856 - 6862, XP055089048, ISSN: 0099-2240, DOI: 10.1128/AEM.71.11.6856-6862.2005 *
See also references of WO2015020690A1 *
SKERRA ET AL: "Bacterial expression of immunoglobulin fragments", CURRENT OPINION IN IMMUNOLOGY, ELSEVIER, OXFORD, GB, vol. 5, no. 2, 1 January 1993 (1993-01-01), pages 256 - 262, XP023942011, ISSN: 0952-7915, [retrieved on 19930101], DOI: 10.1016/0952-7915(93)90014-J *
SORENSEN H P ET AL: "Advanced genetic strategies for recombinant protein expression in Escherichia coli", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 115, no. 2, 26 January 2005 (2005-01-26), pages 113 - 128, XP027663325, ISSN: 0168-1656, [retrieved on 20050126] *
TOMOHIRO MAKINO ET AL: "Strain engineering for improved expression of recombinant proteins in bacteria", MICROBIAL CELL FACTORIES, BIOMED CENTRAL, GB, vol. 10, no. 1, 14 May 2011 (2011-05-14), pages 32, XP021100452, ISSN: 1475-2859, DOI: 10.1186/1475-2859-10-32 *

Also Published As

Publication number Publication date
EP3030651A1 (en) 2016-06-15
WO2015020690A1 (en) 2015-02-12

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Ipc: C12N 9/74 20060101ALI20170119BHEP

Ipc: C12N 15/70 20060101AFI20170119BHEP

Ipc: C12P 21/02 20060101ALI20170119BHEP

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: ARONORA, INC.

Owner name: ABSCI, LLC

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