JP2015519892A5 - - Google Patents
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- Publication number
- JP2015519892A5 JP2015519892A5 JP2015509459A JP2015509459A JP2015519892A5 JP 2015519892 A5 JP2015519892 A5 JP 2015519892A5 JP 2015509459 A JP2015509459 A JP 2015509459A JP 2015509459 A JP2015509459 A JP 2015509459A JP 2015519892 A5 JP2015519892 A5 JP 2015519892A5
- Authority
- JP
- Japan
- Prior art keywords
- adenine dinucleotide
- flavin adenine
- glucose dehydrogenase
- dependent glucose
- modified flavin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000001419 dependent effect Effects 0.000 claims 29
- VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical class C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 claims 29
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 claims 26
- 239000012634 fragment Substances 0.000 claims 20
- 230000013595 glycosylation Effects 0.000 claims 10
- 238000006206 glycosylation reaction Methods 0.000 claims 10
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical class C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 8
- 240000006439 Aspergillus oryzae Species 0.000 claims 7
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 5
- 239000008103 glucose Substances 0.000 claims 5
- 239000002157 polynucleotide Substances 0.000 claims 5
- 102000040430 polynucleotide Human genes 0.000 claims 5
- 108091033319 polynucleotide Proteins 0.000 claims 5
- 238000006467 substitution reaction Methods 0.000 claims 5
- 108090000790 Enzymes Proteins 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 4
- 229940024606 amino acid Drugs 0.000 claims 3
- 235000001014 amino acid Nutrition 0.000 claims 3
- 150000001413 amino acids Chemical class 0.000 claims 3
- 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 claims 3
- 238000001514 detection method Methods 0.000 claims 3
- 235000019162 flavin adenine dinucleotide Nutrition 0.000 claims 3
- 239000011714 flavin adenine dinucleotide Substances 0.000 claims 3
- 229940093632 flavin-adenine dinucleotide Drugs 0.000 claims 3
- 125000003147 glycosyl group Chemical group 0.000 claims 3
- 238000005259 measurement Methods 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 101710088194 Dehydrogenase Proteins 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 230000004988 N-glycosylation Effects 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000018044 dehydration Effects 0.000 claims 1
- 238000006297 dehydration reaction Methods 0.000 claims 1
- 238000006356 dehydrogenation reaction Methods 0.000 claims 1
- 230000008030 elimination Effects 0.000 claims 1
- 238000003379 elimination reaction Methods 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 230000001747 exhibiting effect Effects 0.000 claims 1
- 238000004108 freeze drying Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000002808 molecular sieve Substances 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 claims 1
- 125000003944 tolyl group Chemical group 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12166703.4 | 2012-05-03 | ||
| EP12166703 | 2012-05-03 | ||
| PCT/EP2013/059313 WO2013164477A1 (en) | 2012-05-03 | 2013-05-03 | A glycosylated modified flavin adenine dinucleotide dependent glucose dehydrogenase |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015519892A JP2015519892A (ja) | 2015-07-16 |
| JP2015519892A5 true JP2015519892A5 (https=) | 2016-06-30 |
| JP6258298B2 JP6258298B2 (ja) | 2018-01-10 |
Family
ID=48468244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015509459A Active JP6258298B2 (ja) | 2012-05-03 | 2013-05-03 | グリコシル化されている修飾フラビンアデニンジヌクレオチド依存性グルコース脱水素酵素 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9637774B2 (https=) |
| EP (1) | EP2844747B1 (https=) |
| JP (1) | JP6258298B2 (https=) |
| KR (1) | KR101850178B1 (https=) |
| CN (1) | CN104271736B (https=) |
| CA (1) | CA2869527C (https=) |
| WO (1) | WO2013164477A1 (https=) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5969392B2 (ja) | 2010-12-02 | 2016-08-17 | キッコーマン株式会社 | フラビン結合型グルコースデヒドロゲナーゼ、フラビン結合型グルコースデヒドロゲナーゼの製造方法およびそれに用いる酵母形質転換体 |
| JP6526572B2 (ja) * | 2013-12-27 | 2019-06-05 | キッコーマン株式会社 | 熱安定性が向上したフラビン結合型グルコースデヒドロゲナーゼ |
| CA2993600A1 (en) * | 2015-07-23 | 2017-01-26 | Smartzyme Biopharma Ltd. | Compositions and methods for measuring blood glucose levels |
| JP6901717B2 (ja) | 2016-08-29 | 2021-07-14 | 国立研究開発法人産業技術総合研究所 | グルコースセンサ用試薬、グルコースセンサ、グルコースセンサの製造方法、および、グルコース測定装置 |
| CN110494568B (zh) * | 2017-03-31 | 2024-03-19 | 龟甲万株式会社 | 使用fad依赖性葡糖脱氢酶的连续葡萄糖监测 |
| JP2021078354A (ja) | 2018-03-08 | 2021-05-27 | 有限会社アルティザイム・インターナショナル | フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質 |
| CN108486027A (zh) * | 2018-04-04 | 2018-09-04 | 江南大学 | 一种fad为辅基的葡萄糖脱氢酶的生产、纯化方法 |
| CN112851821B (zh) * | 2021-01-12 | 2022-07-05 | 山东省科学院生物研究所 | 一种融合酶及所述融合酶在纸基生物传感器中的应用 |
| CN113552198B (zh) * | 2021-05-31 | 2024-03-26 | 武汉维尔博生物科技有限公司 | 一种基于高响应葡萄糖适配体的电化学传感器及其制备方法 |
| EP4512395A1 (en) | 2023-08-21 | 2025-02-26 | Bio Even | Composition comprising flavin adenine dinucleotide (fad), l-gsh, atp and myristic acid, alone or with a drug |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2083074B1 (en) * | 2006-11-14 | 2011-01-19 | Toyo Boseki Kabushiki Kaisha | Modified flavine-adenine-dinucleotide-dependent glucose dehydrogenase |
| DE602007012130D1 (de) * | 2006-11-14 | 2011-03-03 | Toyo Boseki | Modifizierte flavin-adenin-dinucleotid-abhängige glucose-dehydrogenase |
| JPWO2008102639A1 (ja) * | 2007-02-20 | 2010-05-27 | 東洋紡績株式会社 | グルコースの電気化学的定量方法、グルコース脱水素酵素組成物、及び、グルコース測定用電気化学センサー |
| EP2241621B1 (en) * | 2007-12-28 | 2012-08-15 | Ikeda Food Research Co. Ltd. | Modified glucose dehydrogenase gene |
| JP5408125B2 (ja) * | 2008-03-27 | 2014-02-05 | 東洋紡株式会社 | 糸状菌由来フラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼ（ｆａｄｇｄｈ） |
| JP2010051312A (ja) * | 2008-08-01 | 2010-03-11 | Toyobo Co Ltd | グルコース脱水素酵素を用いたグルコースの電気化学測定法 |
| JP2010057427A (ja) * | 2008-09-04 | 2010-03-18 | Toyobo Co Ltd | グルコース脱水素酵素およびグルコースの電気化学測定法 |
| EP2426200A4 (en) * | 2009-04-30 | 2012-11-14 | Ikeda Food Res Co Ltd | PROTEIN ELECTRON MEDIATOR |
| JP2012029677A (ja) | 2010-07-08 | 2012-02-16 | Toyobo Co Ltd | フラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼの基質特異性を改善するための方法 |
| JP5811521B2 (ja) | 2010-09-09 | 2015-11-11 | 東洋紡株式会社 | フラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼの比活性を向上するための方法 |
-
2013
- 2013-05-03 CA CA2869527A patent/CA2869527C/en active Active
- 2013-05-03 EP EP13723713.7A patent/EP2844747B1/en active Active
- 2013-05-03 KR KR1020147030170A patent/KR101850178B1/ko active Active
- 2013-05-03 CN CN201380023216.3A patent/CN104271736B/zh active Active
- 2013-05-03 JP JP2015509459A patent/JP6258298B2/ja active Active
- 2013-05-03 WO PCT/EP2013/059313 patent/WO2013164477A1/en not_active Ceased
-
2014
- 2014-10-30 US US14/527,887 patent/US9637774B2/en active Active
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