CA2869527C - A glycosylated modified flavin adenine dinucleotide dependent glucose dehydrogenase - Google Patents

A glycosylated modified flavin adenine dinucleotide dependent glucose dehydrogenase Download PDF

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Publication number
CA2869527C
CA2869527C CA2869527A CA2869527A CA2869527C CA 2869527 C CA2869527 C CA 2869527C CA 2869527 A CA2869527 A CA 2869527A CA 2869527 A CA2869527 A CA 2869527A CA 2869527 C CA2869527 C CA 2869527C
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Prior art keywords
adenine dinucleotide
flavin adenine
glucose dehydrogenase
fad
dependent glucose
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CA2869527A
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English (en)
French (fr)
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CA2869527A1 (en
Inventor
Hartmut Dufel
Thomas Meier
Michael Tacke
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/99—Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
    • C12Y101/9901—Glucose dehydrogenase (acceptor) (1.1.99.10)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/05—Oxidoreductases acting on the CH-OH group of donors (1.1) with a quinone or similar compound as acceptor (1.1.5)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Emergency Medicine (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Diabetes (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2869527A 2012-05-03 2013-05-03 A glycosylated modified flavin adenine dinucleotide dependent glucose dehydrogenase Active CA2869527C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12166703.4 2012-05-03
EP12166703 2012-05-03
PCT/EP2013/059313 WO2013164477A1 (en) 2012-05-03 2013-05-03 A glycosylated modified flavin adenine dinucleotide dependent glucose dehydrogenase

Publications (2)

Publication Number Publication Date
CA2869527A1 CA2869527A1 (en) 2013-11-07
CA2869527C true CA2869527C (en) 2021-05-04

Family

ID=48468244

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2869527A Active CA2869527C (en) 2012-05-03 2013-05-03 A glycosylated modified flavin adenine dinucleotide dependent glucose dehydrogenase

Country Status (7)

Country Link
US (1) US9637774B2 (https=)
EP (1) EP2844747B1 (https=)
JP (1) JP6258298B2 (https=)
KR (1) KR101850178B1 (https=)
CN (1) CN104271736B (https=)
CA (1) CA2869527C (https=)
WO (1) WO2013164477A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5969392B2 (ja) 2010-12-02 2016-08-17 キッコーマン株式会社 フラビン結合型グルコースデヒドロゲナーゼ、フラビン結合型グルコースデヒドロゲナーゼの製造方法およびそれに用いる酵母形質転換体
JP6526572B2 (ja) * 2013-12-27 2019-06-05 キッコーマン株式会社 熱安定性が向上したフラビン結合型グルコースデヒドロゲナーゼ
CA2993600A1 (en) * 2015-07-23 2017-01-26 Smartzyme Biopharma Ltd. Compositions and methods for measuring blood glucose levels
JP6901717B2 (ja) 2016-08-29 2021-07-14 国立研究開発法人産業技術総合研究所 グルコースセンサ用試薬、グルコースセンサ、グルコースセンサの製造方法、および、グルコース測定装置
CN110494568B (zh) * 2017-03-31 2024-03-19 龟甲万株式会社 使用fad依赖性葡糖脱氢酶的连续葡萄糖监测
JP2021078354A (ja) 2018-03-08 2021-05-27 有限会社アルティザイム・インターナショナル フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質
CN108486027A (zh) * 2018-04-04 2018-09-04 江南大学 一种fad为辅基的葡萄糖脱氢酶的生产、纯化方法
CN112851821B (zh) * 2021-01-12 2022-07-05 山东省科学院生物研究所 一种融合酶及所述融合酶在纸基生物传感器中的应用
CN113552198B (zh) * 2021-05-31 2024-03-26 武汉维尔博生物科技有限公司 一种基于高响应葡萄糖适配体的电化学传感器及其制备方法
EP4512395A1 (en) 2023-08-21 2025-02-26 Bio Even Composition comprising flavin adenine dinucleotide (fad), l-gsh, atp and myristic acid, alone or with a drug

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2083074B1 (en) * 2006-11-14 2011-01-19 Toyo Boseki Kabushiki Kaisha Modified flavine-adenine-dinucleotide-dependent glucose dehydrogenase
DE602007012130D1 (de) * 2006-11-14 2011-03-03 Toyo Boseki Modifizierte flavin-adenin-dinucleotid-abhängige glucose-dehydrogenase
JPWO2008102639A1 (ja) * 2007-02-20 2010-05-27 東洋紡績株式会社 グルコースの電気化学的定量方法、グルコース脱水素酵素組成物、及び、グルコース測定用電気化学センサー
EP2241621B1 (en) * 2007-12-28 2012-08-15 Ikeda Food Research Co. Ltd. Modified glucose dehydrogenase gene
JP5408125B2 (ja) * 2008-03-27 2014-02-05 東洋紡株式会社 糸状菌由来フラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼ（ｆａｄｇｄｈ）
JP2010051312A (ja) * 2008-08-01 2010-03-11 Toyobo Co Ltd グルコース脱水素酵素を用いたグルコースの電気化学測定法
JP2010057427A (ja) * 2008-09-04 2010-03-18 Toyobo Co Ltd グルコース脱水素酵素およびグルコースの電気化学測定法
EP2426200A4 (en) * 2009-04-30 2012-11-14 Ikeda Food Res Co Ltd PROTEIN ELECTRON MEDIATOR
JP2012029677A (ja) 2010-07-08 2012-02-16 Toyobo Co Ltd フラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼの基質特異性を改善するための方法
JP5811521B2 (ja) 2010-09-09 2015-11-11 東洋紡株式会社 フラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼの比活性を向上するための方法

Also Published As

Publication number Publication date
CN104271736A (zh) 2015-01-07
CN104271736B (zh) 2020-06-05
JP6258298B2 (ja) 2018-01-10
KR20150001775A (ko) 2015-01-06
HK1205757A1 (en) 2015-12-24
KR101850178B1 (ko) 2018-05-31
US9637774B2 (en) 2017-05-02
CA2869527A1 (en) 2013-11-07
JP2015519892A (ja) 2015-07-16
EP2844747B1 (en) 2019-03-20
EP2844747A1 (en) 2015-03-11
US20150064733A1 (en) 2015-03-05
WO2013164477A1 (en) 2013-11-07

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