JP2015516161A5 - - Google Patents

Info

Publication number

JP2015516161A5

JP2015516161A5 JP2015511622A JP2015511622A JP2015516161A5 JP 2015516161 A5 JP2015516161 A5 JP 2015516161A5 JP 2015511622 A JP2015511622 A JP 2015511622A JP 2015511622 A JP2015511622 A JP 2015511622A JP 2015516161 A5 JP2015516161 A5 JP 2015516161A5

Authority

JP

Japan

Prior art keywords

cells

marker

indicator

expressing

cell

Prior art date

2013-05-07

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 210000001900 endoderm Anatomy 0.000 claims description 34
• 238000000034 method Methods 0.000 claims description 29
• 238000010899 nucleation Methods 0.000 claims description 21
• 210000001778 pluripotent stem cell Anatomy 0.000 claims description 21
• 210000001671 embryonic stem cell Anatomy 0.000 claims description 7
• 108010082117 matrigel Proteins 0.000 claims description 4
• 210000000130 stem cell Anatomy 0.000 claims description 3
• 239000003550 marker Substances 0.000 claims 37
• 230000004069 differentiation Effects 0.000 claims 2
• 210000003372 endocrine gland Anatomy 0.000 claims 2
• 210000004039 endoderm cell Anatomy 0.000 claims 2
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 2
• 230000009996 pancreatic endocrine effect Effects 0.000 claims 2
• 108010083123 CDX2 Transcription Factor Proteins 0.000 claims 1
• 101100518002 Danio rerio nkx2.2a gene Proteins 0.000 claims 1
• 108700014808 Homeobox Protein Nkx-2.2 Proteins 0.000 claims 1
• 102100031671 Homeobox protein CDX-2 Human genes 0.000 claims 1
• 102100027886 Homeobox protein Nkx-2.2 Human genes 0.000 claims 1
• 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims 1
• 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims 1
• 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims 1
• 101100460496 Homo sapiens NKX2-2 gene Proteins 0.000 claims 1
• 101000603702 Homo sapiens Neurogenin-3 Proteins 0.000 claims 1
• 101000976653 Homo sapiens Zinc finger protein ZIC 1 Proteins 0.000 claims 1
• 102000004877 Insulin Human genes 0.000 claims 1
• 108090001061 Insulin Proteins 0.000 claims 1
• 102100032063 Neurogenic differentiation factor 1 Human genes 0.000 claims 1
• 108050000588 Neurogenic differentiation factor 1 Proteins 0.000 claims 1
• 102100038553 Neurogenin-3 Human genes 0.000 claims 1
• 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 claims 1
• 101710144033 Pancreas/duodenum homeobox protein 1 Proteins 0.000 claims 1
• 102100023497 Zinc finger protein ZIC 1 Human genes 0.000 claims 1
• 238000012258 culturing Methods 0.000 claims 1
• 210000001654 germ layer Anatomy 0.000 claims 1
• 238000000338 in vitro Methods 0.000 claims 1
• 229940125396 insulin Drugs 0.000 claims 1
• 230000003827 upregulation Effects 0.000 claims 1
• 239000002609 medium Substances 0.000 description 6
• HJCMDXDYPOUFDY-WHFBIAKZSA-N Ala-Gln Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)CCC(N)=O HJCMDXDYPOUFDY-WHFBIAKZSA-N 0.000 description 2
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
• 108060003393 Granulin Proteins 0.000 description 2
• 102100039939 Growth/differentiation factor 8 Human genes 0.000 description 2
• 101000886562 Homo sapiens Growth/differentiation factor 8 Proteins 0.000 description 2
• UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
• 229910052791 calcium Inorganic materials 0.000 description 2
• 239000011575 calcium Substances 0.000 description 2
• 235000014113 dietary fatty acids Nutrition 0.000 description 2
• 238000005516 engineering process Methods 0.000 description 2
• 229930195729 fatty acid Natural products 0.000 description 2
• 239000000194 fatty acid Substances 0.000 description 2
• 150000004665 fatty acids Chemical class 0.000 description 2
• 239000003112 inhibitor Substances 0.000 description 2
• 239000000203 mixture Substances 0.000 description 2
• 239000002953 phosphate buffered saline Substances 0.000 description 2
• OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
• 102000004190 Enzymes Human genes 0.000 description 1
• 108090000790 Enzymes Proteins 0.000 description 1
• 102000019058 Glycogen Synthase Kinase 3 beta Human genes 0.000 description 1
• 108010051975 Glycogen Synthase Kinase 3 beta Proteins 0.000 description 1
• 241000288906 Primates Species 0.000 description 1
• UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
• 230000010261 cell growth Effects 0.000 description 1
• 239000002738 chelating agent Substances 0.000 description 1
• 150000001875 compounds Chemical class 0.000 description 1
• 239000003636 conditioned culture medium Substances 0.000 description 1
• LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
• 210000003917 human chromosome Anatomy 0.000 description 1
• 238000002454 metastable transfer emission spectrometry Methods 0.000 description 1
• 239000011435 rock Substances 0.000 description 1
• 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
• 235000017557 sodium bicarbonate Nutrition 0.000 description 1
• 239000013589 supplement Substances 0.000 description 1
• 239000000725 suspension Substances 0.000 description 1
• CPUDPFPXCZDNGI-UHFFFAOYSA-N triethoxy(methyl)silane Chemical compound CCO[Si](C)(OCC)OCC CPUDPFPXCZDNGI-UHFFFAOYSA-N 0.000 description 1