AU2013259706A1 - Differentiation of human embryonic stem cells into pancreatic endoderm - Google Patents

Differentiation of human embryonic stem cells into pancreatic endoderm Download PDF

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Publication number
AU2013259706A1
AU2013259706A1 AU2013259706A AU2013259706A AU2013259706A1 AU 2013259706 A1 AU2013259706 A1 AU 2013259706A1 AU 2013259706 A AU2013259706 A AU 2013259706A AU 2013259706 A AU2013259706 A AU 2013259706A AU 2013259706 A1 AU2013259706 A1 AU 2013259706A1
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Prior art keywords
cells
expressing markers
stem cells
markers indicative
definitive endoderm
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Abandoned
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AU2013259706A
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English (en)
Inventor
Alireza Rezania
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Janssen Biotech Inc
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Janssen Biotech Inc
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Publication date
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of AU2013259706A1 publication Critical patent/AU2013259706A1/en
Abandoned legal-status Critical Current

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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0676Pancreatic cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0676Pancreatic cells
    • C12N5/0678Stem cells; Progenitor cells; Precursor cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/005Protein-free medium
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/067Hepatocytes
    • C12N5/0672Stem cells; Progenitor cells; Precursor cells; Oval cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475Growth factors; Growth regulators
    • C07K14/505Erythropoietin [EPO]
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/065Modulators of histone acetylation
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/19Growth and differentiation factors [GDF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999Small molecules not provided for elsewhere
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production
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    • C12N2511/00Cells for large scale production
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2013259706A 2012-05-07 2013-05-07 Differentiation of human embryonic stem cells into pancreatic endoderm Abandoned AU2013259706A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261643684P 2012-05-07 2012-05-07
US61/643,684 2012-05-07
PCT/US2013/039940 WO2013169769A1 (en) 2012-05-07 2013-05-07 Differentiation of human embryonic stem cells into pancreatic endoderm

Publications (1)

Publication Number Publication Date
AU2013259706A1 true AU2013259706A1 (en) 2014-10-30

Family

ID=49551213

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2013259706A Abandoned AU2013259706A1 (en) 2012-05-07 2013-05-07 Differentiation of human embryonic stem cells into pancreatic endoderm

Country Status (17)

Country Link
US (1) US20140162359A1 (enExample)
EP (1) EP2847319A4 (enExample)
JP (1) JP6450674B2 (enExample)
KR (1) KR20150014478A (enExample)
CN (1) CN104284977A (enExample)
AR (1) AR090970A1 (enExample)
AU (1) AU2013259706A1 (enExample)
BR (1) BR112014027783A2 (enExample)
CA (1) CA2872770A1 (enExample)
HK (1) HK1207885A1 (enExample)
IL (1) IL235132A0 (enExample)
IN (1) IN2014DN08561A (enExample)
MX (1) MX2014013524A (enExample)
PH (1) PH12014502686A1 (enExample)
RU (1) RU2668814C2 (enExample)
SG (1) SG11201406884SA (enExample)
WO (1) WO2013169769A1 (enExample)

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KR102341674B1 (ko) 2013-06-11 2021-12-22 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 SC-β 세포 및 조성물 그리고 그 생성 방법
CN113234661A (zh) 2014-12-18 2021-08-10 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
US10190096B2 (en) 2014-12-18 2019-01-29 President And Fellows Of Harvard College Methods for generating stem cell-derived β cells and uses thereof
WO2016100898A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived b cells and uses thereof
CN106467918B (zh) * 2015-08-18 2020-07-31 中国科学技术大学先进技术研究院 一种基于人皮肤细胞的胰岛素分泌细胞的诱导方法及应用
US20210147807A1 (en) * 2017-06-14 2021-05-20 Helmholtz Zentrum Munchen - Deutsches Forschungszentrum Fur Gesundheit Und Umwelt (Gmbh) Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
KR102789149B1 (ko) 2017-11-15 2025-03-31 셈마 테라퓨틱스, 인크. 섬세포 제조 조성물 및 사용 방법
AU2019320072A1 (en) 2018-08-10 2021-02-25 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
US12378572B2 (en) 2018-09-07 2025-08-05 Crispr Therapeutics Ag Universal donor cells
AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
WO2020243666A1 (en) 2019-05-31 2020-12-03 W. L. Gore & Associates, Inc. A biocompatible membrane composite
EP3976237A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates Inc. Cell encapsulation devices with controlled oxygen diffusion distances
EP3976236A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates Inc. A biocompatible membrane composite
WO2021044377A1 (en) 2019-09-05 2021-03-11 Crispr Therapeutics Ag Universal donor cells
US11118196B2 (en) 2019-09-05 2021-09-14 Crispr Therapeutics Ag Universal donor cells
WO2022144856A1 (en) 2020-12-31 2022-07-07 Crispr Therapeutics Ag Universal donor cells
CN113046306B (zh) * 2021-03-12 2022-10-18 广东东阳光药业有限公司 一种多能干细胞的培养方法

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CA2695225C (en) * 2007-07-31 2021-06-01 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic endocrine
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Also Published As

Publication number Publication date
WO2013169769A1 (en) 2013-11-14
AR090970A1 (es) 2014-12-17
CA2872770A1 (en) 2013-11-14
PH12014502686A1 (en) 2015-01-26
RU2668814C2 (ru) 2018-10-02
EP2847319A1 (en) 2015-03-18
JP6450674B2 (ja) 2019-01-09
CN104284977A (zh) 2015-01-14
RU2014149185A (ru) 2016-06-27
IN2014DN08561A (enExample) 2015-05-22
JP2015516161A (ja) 2015-06-11
SG11201406884SA (en) 2014-11-27
IL235132A0 (en) 2014-12-31
BR112014027783A2 (pt) 2017-06-27
MX2014013524A (es) 2015-02-10
KR20150014478A (ko) 2015-02-06
HK1207885A1 (en) 2016-02-12
EP2847319A4 (en) 2015-12-16
US20140162359A1 (en) 2014-06-12

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