JP2015514404A5 - - Google Patents
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- Publication number
- JP2015514404A5 JP2015514404A5 JP2015506048A JP2015506048A JP2015514404A5 JP 2015514404 A5 JP2015514404 A5 JP 2015514404A5 JP 2015506048 A JP2015506048 A JP 2015506048A JP 2015506048 A JP2015506048 A JP 2015506048A JP 2015514404 A5 JP2015514404 A5 JP 2015514404A5
- Authority
- JP
- Japan
- Prior art keywords
- polynucleotide
- birds
- embryo
- germ cells
- transfection reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 21
- 241000271566 Aves Species 0.000 claims 15
- 108091033319 polynucleotide Proteins 0.000 claims 12
- 239000002157 polynucleotide Substances 0.000 claims 12
- 102000040430 polynucleotide Human genes 0.000 claims 12
- 210000001161 mammalian embryo Anatomy 0.000 claims 10
- 210000004602 germ cell Anatomy 0.000 claims 9
- 239000012096 transfection reagent Substances 0.000 claims 7
- 239000000203 mixture Substances 0.000 claims 5
- 238000001890 transfection Methods 0.000 claims 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 3
- PFNFFQXMRSDOHW-UHFFFAOYSA-N Spermine Natural products NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 claims 3
- 210000004204 blood vessel Anatomy 0.000 claims 3
- 230000013011 mating Effects 0.000 claims 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims 3
- KSXTUUUQYQYKCR-LQDDAWAPSA-M 2,3-bis[[(z)-octadec-9-enoyl]oxy]propyl-trimethylazanium;chloride Chemical compound [Cl-].CCCCCCCC\C=C/CCCCCCCC(=O)OCC(C[N+](C)(C)C)OC(=O)CCCCCCC\C=C/CCCCCCCC KSXTUUUQYQYKCR-LQDDAWAPSA-M 0.000 claims 2
- QPYUGOUSOOCGGQ-UHFFFAOYSA-N CN(CCCN(CCCCN(CCC(N(CCCCCCCCCCCC)CCCCCCCCCCCC)(CCCCCCCCCCCC)CCCCCCCCCCCC)C)C)C Chemical compound CN(CCCN(CCCCN(CCC(N(CCCCCCCCCCCC)CCCCCCCCCCCC)(CCCCCCCCCCCC)CCCCCCCCCCCC)C)C)C QPYUGOUSOOCGGQ-UHFFFAOYSA-N 0.000 claims 2
- 125000002091 cationic group Chemical group 0.000 claims 2
- -1 cationic lipid Chemical class 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- 229940063675 spermine Drugs 0.000 claims 2
- 230000029812 viral genome replication Effects 0.000 claims 2
- LDGWQMRUWMSZIU-LQDDAWAPSA-M 2,3-bis[(z)-octadec-9-enoxy]propyl-trimethylazanium;chloride Chemical group [Cl-].CCCCCCCC\C=C/CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC\C=C/CCCCCCCC LDGWQMRUWMSZIU-LQDDAWAPSA-M 0.000 claims 1
- WALUVDCNGPQPOD-UHFFFAOYSA-M 2,3-di(tetradecoxy)propyl-(2-hydroxyethyl)-dimethylazanium;bromide Chemical compound [Br-].CCCCCCCCCCCCCCOCC(C[N+](C)(C)CCO)OCCCCCCCCCCCCCC WALUVDCNGPQPOD-UHFFFAOYSA-M 0.000 claims 1
- 241000272525 Anas platyrhynchos Species 0.000 claims 1
- 241000272814 Anser sp. Species 0.000 claims 1
- RBGCWNFXPQALRC-UHFFFAOYSA-N CN(CCCN(CCCCN(CCC(N(C(CCCCCCCCCCCCCCC)=O)C(CCCCCCCCCCCCCCC)=O)(C(CCCCCCCCCCCCCCC)=O)C(CCCCCCCCCCCCCCC)=O)C)C)C Chemical compound CN(CCCN(CCCCN(CCC(N(C(CCCCCCCCCCCCCCC)=O)C(CCCCCCCCCCCCCCC)=O)(C(CCCCCCCCCCCCCCC)=O)C(CCCCCCCCCCCCCCC)=O)C)C)C RBGCWNFXPQALRC-UHFFFAOYSA-N 0.000 claims 1
- NFIDGYIHSCPMMK-SDLHUVDUSA-N CN(CCCN(CCCCN(CCC(N(CCCCCCCC\C=C/CCCCCCCC)CCCCCCCC\C=C/CCCCCCCC)(CCCCCCCC\C=C/CCCCCCCC)CCCCCCCC\C=C/CCCCCCCC)C)C)C Chemical compound CN(CCCN(CCCCN(CCC(N(CCCCCCCC\C=C/CCCCCCCC)CCCCCCCC\C=C/CCCCCCCC)(CCCCCCCC\C=C/CCCCCCCC)CCCCCCCC\C=C/CCCCCCCC)C)C)C NFIDGYIHSCPMMK-SDLHUVDUSA-N 0.000 claims 1
- 108091033409 CRISPR Proteins 0.000 claims 1
- 238000010354 CRISPR gene editing Methods 0.000 claims 1
- XULFJDKZVHTRLG-JDVCJPALSA-N DOSPA trifluoroacetate Chemical group [O-]C(=O)C(F)(F)F.CCCCCCCC\C=C/CCCCCCCCOCC(C[N+](C)(C)CCNC(=O)C(CCCNCCCN)NCCCN)OCCCCCCCC\C=C/CCCCCCCC XULFJDKZVHTRLG-JDVCJPALSA-N 0.000 claims 1
- 102000002322 Egg Proteins Human genes 0.000 claims 1
- 108010000912 Egg Proteins Proteins 0.000 claims 1
- 241000287828 Gallus gallus Species 0.000 claims 1
- 241000286209 Phasianidae Species 0.000 claims 1
- 108091027967 Small hairpin RNA Proteins 0.000 claims 1
- 108020004459 Small interfering RNA Proteins 0.000 claims 1
- 238000010459 TALEN Methods 0.000 claims 1
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 claims 1
- DTQVDTLACAAQTR-UHFFFAOYSA-M Trifluoroacetate Chemical compound [O-]C(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-M 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims 1
- 230000000840 anti-viral effect Effects 0.000 claims 1
- UMGXUWVIJIQANV-UHFFFAOYSA-M didecyl(dimethyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCC[N+](C)(C)CCCCCCCCCC UMGXUWVIJIQANV-UHFFFAOYSA-M 0.000 claims 1
- PSLWZOIUBRXAQW-UHFFFAOYSA-M dimethyl(dioctadecyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCCCCCCCCCCCCCCCC PSLWZOIUBRXAQW-UHFFFAOYSA-M 0.000 claims 1
- 210000003278 egg shell Anatomy 0.000 claims 1
- 210000002257 embryonic structure Anatomy 0.000 claims 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 230000007935 neutral effect Effects 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 125000002811 oleoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])/C([H])=C([H])\C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 1
- 239000004055 small Interfering RNA Substances 0.000 claims 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261636331P | 2012-04-20 | 2012-04-20 | |
| US61/636,331 | 2012-04-20 | ||
| US201361783823P | 2013-03-14 | 2013-03-14 | |
| US61/783,823 | 2013-03-14 | ||
| AU2013204327 | 2013-04-12 | ||
| AU2013204327A AU2013204327B2 (en) | 2012-04-20 | 2013-04-12 | Cell transfection method |
| PCT/AU2013/000414 WO2013155572A1 (en) | 2012-04-20 | 2013-04-19 | Cell transfection method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015514404A JP2015514404A (ja) | 2015-05-21 |
| JP2015514404A5 true JP2015514404A5 (enExample) | 2016-06-16 |
| JP6531239B2 JP6531239B2 (ja) | 2019-06-19 |
Family
ID=49515073
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015506048A Active JP6531239B2 (ja) | 2012-04-20 | 2013-04-19 | 細胞トランスフェクション法 |
Country Status (9)
| Country | Link |
|---|---|
| US (5) | US10897881B2 (enExample) |
| EP (2) | EP3460065B1 (enExample) |
| JP (1) | JP6531239B2 (enExample) |
| KR (1) | KR102076784B1 (enExample) |
| CN (2) | CN104619848B (enExample) |
| AU (2) | AU2013204327B2 (enExample) |
| BR (1) | BR112014026186B8 (enExample) |
| ES (2) | ES2704155T3 (enExample) |
| WO (1) | WO2013155572A1 (enExample) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| AU2013204327B2 (en) * | 2012-04-20 | 2016-09-01 | Aviagen | Cell transfection method |
| MY189533A (en) | 2012-05-25 | 2022-02-16 | Univ California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| JP6342910B2 (ja) | 2012-10-29 | 2018-06-13 | エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ | 遺伝子薬物療法のための新規試薬 |
| AU2013355214B2 (en) | 2012-12-06 | 2017-06-15 | Sigma-Aldrich Co. Llc | Crispr-based genome modification and regulation |
| EP4299741A3 (en) | 2012-12-12 | 2024-02-28 | The Broad Institute, Inc. | Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
| WO2014093701A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| WO2014093709A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
| WO2014150624A1 (en) | 2013-03-14 | 2014-09-25 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
| DK3011029T3 (da) | 2013-06-17 | 2020-03-16 | Broad Inst Inc | Administration, modificering og optimering af tandem-guidesystemer, fremgangsmåder og sammensætninger til sekvensmanipulering |
| EP3011031B1 (en) | 2013-06-17 | 2020-09-30 | The Broad Institute Inc. | Delivery and use of the crispr-cas systems, vectors and compositions for hepatic targeting and therapy |
| CN105492611A (zh) | 2013-06-17 | 2016-04-13 | 布罗德研究所有限公司 | 用于序列操纵的优化的crispr-cas双切口酶系统、方法以及组合物 |
| KR20160056869A (ko) | 2013-06-17 | 2016-05-20 | 더 브로드 인스티튜트, 인코퍼레이티드 | 바이러스 구성성분을 사용하여 장애 및 질환을 표적화하기 위한 crispr-cas 시스템 및 조성물의 전달, 용도 및 치료 적용 |
| WO2014204727A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof |
| CN106103705A (zh) | 2013-12-12 | 2016-11-09 | 布罗德研究所有限公司 | 核苷酸重复障碍中crispr‑cas系统的组合物和使用方法 |
| WO2015089419A2 (en) * | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components |
| WO2015089473A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation |
| WO2015089364A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Crystal structure of a crispr-cas system, and uses thereof |
| KR20250068794A (ko) | 2013-12-12 | 2025-05-16 | 더 브로드 인스티튜트, 인코퍼레이티드 | 게놈 편집을 위한 ｃｒｉｓｐｒ-ｃａｓ 시스템 및 조성물의 전달, 용도 및 치료적 응용 |
| WO2015089486A2 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems |
| CA2932479A1 (en) | 2013-12-12 | 2015-06-18 | The Rockefeller University | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for hbv and viral diseases and disorders |
| CN103782953A (zh) * | 2014-01-23 | 2014-05-14 | 固镇县玉鹏蔬菜开发有限责任公司 | 一种小鹅瘟的预防方法 |
| CN105265383A (zh) * | 2014-10-18 | 2016-01-27 | 颍上县永祥旱粮研究所 | 一种林下鸡养殖方法 |
| WO2016094867A1 (en) | 2014-12-12 | 2016-06-16 | The Broad Institute Inc. | Protected guide rnas (pgrnas) |
| WO2016106244A1 (en) | 2014-12-24 | 2016-06-30 | The Broad Institute Inc. | Crispr having or associated with destabilization domains |
| CN104789599A (zh) * | 2015-04-13 | 2015-07-22 | 浙江省农业科学院 | 一种提高慢病毒胚盘下腔注射制备转基因家禽效率的方法 |
| KR102840885B1 (ko) | 2015-06-18 | 2025-07-30 | 더 브로드 인스티튜트, 인코퍼레이티드 | 표적외 효과를 감소시키는 crispr 효소 돌연변이 |
| WO2016205759A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
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| JP6710825B2 (ja) | 2015-12-25 | 2020-06-17 | 国立研究開発法人産業技術総合研究所 | 遺伝子改変家禽卵 |
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| JP7418470B2 (ja) * | 2019-04-08 | 2024-01-19 | ディーエヌエー ツーポイントオー インク． | オリジアス由来のトランスポザーゼを用いた核酸コンストラクトの真核細胞への組み込み |
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| TW202241259A (zh) | 2020-12-31 | 2022-11-01 | 以色列國家農業部、農村發展農業研究組織（沃爾卡尼研究所） | 不育禽類胚胎、其產生及用途 |
-
2013
- 2013-04-12 AU AU2013204327A patent/AU2013204327B2/en active Active
- 2013-04-19 BR BR112014026186A patent/BR112014026186B8/pt active IP Right Grant
- 2013-04-19 ES ES13777737T patent/ES2704155T3/es active Active
- 2013-04-19 KR KR1020147031173A patent/KR102076784B1/ko active Active
- 2013-04-19 AU AU2013248945A patent/AU2013248945A1/en not_active Abandoned
- 2013-04-19 CN CN201380031734.XA patent/CN104619848B/zh active Active
- 2013-04-19 CN CN202011320159.3A patent/CN112626128B/zh active Active
- 2013-04-19 US US14/394,712 patent/US10897881B2/en active Active
- 2013-04-19 EP EP18187574.1A patent/EP3460065B1/en active Active
- 2013-04-19 WO PCT/AU2013/000414 patent/WO2013155572A1/en not_active Ceased
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- 2013-04-19 ES ES18187574T patent/ES2963732T3/es active Active
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2019
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2025
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