JP7493940B2 - 鳥類における形質選択 - Google Patents
鳥類における形質選択 Download PDFInfo
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- JP7493940B2 JP7493940B2 JP2019566285A JP2019566285A JP7493940B2 JP 7493940 B2 JP7493940 B2 JP 7493940B2 JP 2019566285 A JP2019566285 A JP 2019566285A JP 2019566285 A JP2019566285 A JP 2019566285A JP 7493940 B2 JP7493940 B2 JP 7493940B2
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Description
i)Z染色体上にあってその卵の中で検出可能なマーカーをコードしている第1の遺伝子改変と;
ii)同じZ染色体上にあって、その卵の中、および/またはその卵から生まれた鳥類の中で産生形質を変化させる第2の遺伝子改変を、その第2の遺伝子改変が欠けた同質遺伝子型の卵または鳥類と比べたときに含んでいる、トランスジェニック鳥類の卵が提供される。
i)Z染色体上にあってその鳥類が産んだ卵の中で検出可能なマーカーをコードしている第1の遺伝子改変と;
ii)同じZ染色体上にあってその卵の中、および/またはその卵から生まれた鳥類の中で産生形質を変化させる第2の遺伝子改変を、その第2の遺伝子改変が欠けた同質遺伝子型の卵または鳥類と比べて含む鳥類が提供される。
i)本発明の雌のトランスジェニック鳥類を、第1の遺伝子改変が欠けた雄の鳥類と交配させることによって産まれた鳥類の卵を取得し、
ii)その卵をマーカーに関してスクリーニングすることを含んでいて、
その卵がマーカーを有する場合に雄である方法が提供される。
i)本明細書に記載されている雌の鳥類を雄の鳥類と交配させて鳥類の卵を取得し、
ii)その卵をマーカーに関してスクリーニングし、
iii)雄性卵を雌性卵から分離することを含み、
卵がマーカーを有する場合に雄であり、卵がマーカーを欠いている場合に雌である方法が提供される。
(i)雌の鳥類を雄の鳥類と交配させることにより、少なくとも1羽が本発明の鳥類である鳥類を取得し、
(ii)その鳥類から食肉および/または卵を回収する方法が提供される。
1)本明細書に記載されている鳥類の卵を取得し、
2)その卵にウイルスを接種し、
3)その卵を所定の期間にわたって孵卵してそのウイルスを複製することを含む方法が提供される。
1)本明細書に記載されている方法を利用してウイルスを複製し、
2)複製されたそのウイルスまたはその粒子を卵から回収し、
3)回収されたそのウイルスからワクチン組成物を調製することを含む方法が提供される。
i)Z染色体上にあって鳥類の卵の中で検出可能なマーカーをコードしている第1の遺伝子改変と;
ii)同じZ染色体上にあってその卵の中、および/またはその卵から生まれた鳥類の中で産生形質を変化させる第2の遺伝子改変を、その第2の遺伝子改変が欠けた同質遺伝子型の卵または鳥類と比べて含んでいて、
この方法が、遺伝子改変に関してヘテロ接合型である雄の鳥類を、Z染色体上に前記遺伝子改変を含む雌の鳥類と交配させて、遺伝子改変に関してホモ接合型である雄性卵を産ませること、またはその卵から鳥類を生まれさせることを含む方法が提供される。
i)Z染色体上にあって鳥類の卵の中で検出可能なマーカーをコードしている第1の遺伝子改変と;
ii)同じZ染色体上にあってその卵の中、および/またはその卵から生まれた鳥類の中で産生形質を変化させる第2の遺伝子改変を、その第2の遺伝子改変が欠けた同質遺伝子型の卵または鳥類と比べて含んでいて、
この方法が、遺伝子改変に関してホモ接合型である雄の鳥類を、遺伝子改変が欠けた雌の鳥類と交配させて、Z染色体上に遺伝子改変を含む雌性卵を産ませること、またはその卵から鳥類を生まれさせることを含む方法が提供される。
i)Z染色体上にあって鳥類の卵の中で検出可能なマーカーをコードしている第1の遺伝子改変と;
ii)同じZ染色体上にあってその卵の中、および/またはその卵から生まれた鳥類の中で産生形質を変化させる第2の遺伝子改変を、その第2の遺伝子改変が欠けた同質遺伝子型の卵または鳥類と比べて含んでいて、
この方法が、Z染色体上に遺伝子改変を含む雌の鳥類を、遺伝子改変が欠けた雄の鳥類と交配させて、遺伝子改変に関してヘテロ接合型である雄性卵を産ませること、またはその卵から鳥類を生まれさせることを含み、この交配から産まれた雌性卵、またはこの卵から生まれた鳥類は、遺伝子改変を欠いている方法が提供される。
i)Z染色体上にあって鳥類の卵の中で検出可能なマーカーをコードしている第1の遺伝子改変と;
ii)同じZ染色体上にあってその卵の中、および/またはその卵から生まれた鳥類の中で産生形質を変化させる第2の遺伝子改変を、その第2の遺伝子改変が欠けた同質遺伝子型の卵または鳥類と比べて含み、
1)遺伝子改変に関してヘテロである雄の鳥類を、Z染色体上に遺伝子改変を含む雌の鳥類と交配させて、遺伝子改変に関してヘテロ接合型である雄の鳥類を生まれさせ、
2)工程1)で生まれた雄の鳥類を、遺伝子改変が欠けた雌の鳥類と交配させて、Z染色体上に遺伝子改変を含む雌の鳥類を生まれさせ、
3)工程2)で生まれた雌の鳥類を、遺伝子改変が欠けた雄の鳥類と交配させて、遺伝子改変に関してヘテロ接合型である雄性卵を産ませ、またはその卵から鳥類を生まれさせ、この交配から産まれた雌性卵、またはこの卵から生まれた鳥類は、遺伝子改変を欠いている方法が提供される。
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配列ID番号:53:si-DDI2-3センスヌクレオチド配列
配列ID番号:54:si-DDI2-3アンチセンスヌクレオチド配列
配列ID番号:55:si-DDI2-4センスヌクレオチド配列
配列ID番号:56:si-DDI2-4アンチセンスヌクレオチド配列
配列ID番号:57:si-LOC100859339-1センスヌクレオチド配列
配列ID番号:58:si-LOC100859339-1アンチセンスヌクレオチド配列
配列ID番号:59:si-LOC100859339-2センスヌクレオチド配列
配列ID番号:60:si-LOC100859339-2アンチセンスヌクレオチド配列
配列ID番号:61:si-LOC100859339-3センスヌクレオチド配列
配列ID番号:62:si-LOC100859339-3アンチセンスヌクレオチド配列
配列ID番号:63:si-LOC100859339-4センスヌクレオチド配列
配列ID番号:64:si-LOC100859339-4アンチセンスヌクレオチド配列
配列ID番号:65:si-CNOT4-1センスヌクレオチド配列
配列ID番号:66:si-CNOT4-1アンチセンスヌクレオチド配列
配列ID番号:67:si-CNOT4-2センスヌクレオチド配列
配列ID番号:68:si-CNOT4-2アンチセンスヌクレオチド配列
配列ID番号:69:si-CNOT4-3センスヌクレオチド配列
配列ID番号:70:si-CNOT4-3アンチセンスヌクレオチド配列
配列ID番号:71:si-CNOT4-4センスヌクレオチド配列
配列ID番号:72:si-CNOT4-4アンチセンスヌクレオチド配列
配列ID番号:73:si-CAPN13-1センスヌクレオチド配列
配列ID番号:74:si-CAPN13-1アンチセンスヌクレオチド配列
配列ID番号:75:si-CAPN13-2センスヌクレオチド配列
配列ID番号:76:si-CAPN13-2アンチセンスヌクレオチド配列
配列ID番号:77:si-CAPN13-3センスヌクレオチド配列
配列ID番号:78:si-CAPN13-3アンチセンスヌクレオチド配列
配列ID番号:79:si-CAPN13-4センスヌクレオチド配列
配列ID番号:80:si-CAPN13-4アンチセンスヌクレオチド配列
配列ID番号:81:si-DNASE1L2-1センスヌクレオチド配列
配列ID番号:82:si-DNASE1L2-1アンチセンスヌクレオチド配列
配列ID番号:83:si-DNASE1L2-2センスヌクレオチド配列
配列ID番号:84:si-DNASE1L2-2アンチセンスヌクレオチド配列
配列ID番号:85:si-DNASE1L2-3センスヌクレオチド配列
配列ID番号:86:si-DNASE1L2-3アンチセンスヌクレオチド配列
配列ID番号:87:si-DNASE1L2-4センスヌクレオチド配列
配列ID番号:88:si-DNASE1L2-4アンチセンスヌクレオチド配列
配列ID番号:89:si-PHF21A-1センスヌクレオチド配列
配列ID番号:90:si-PHF21A-1アンチセンスヌクレオチド配列
配列ID番号:91:si-PHF21A-2センスヌクレオチド配列
配列ID番号:92:si-PHF21A-2アンチセンスヌクレオチド配列
配列ID番号:93:si-PHF21A-3センスヌクレオチド配列
配列ID番号:94:si-PHF21A-3アンチセンスヌクレオチド配列
配列ID番号:95:si-PHF21A-4センスヌクレオチド配列
配列ID番号:96:si-PHF21A-4アンチセンスヌクレオチド配列
配列ID番号:97:si-PCGF5-1センスヌクレオチド配列
配列ID番号:98:si-PCGF5-1アンチセンスヌクレオチド配列
配列ID番号:99:si-PCGF5-2センスヌクレオチド配列
配列ID番号:100:si-PCGF5-2アンチセンスヌクレオチド配列
配列ID番号:101:si-PCGF5-3センスヌクレオチド配列
配列ID番号:102:si-PCGF5-3アンチセンスヌクレオチド配列
配列ID番号:103:si-PCGF5-4センスヌクレオチド配列
配列ID番号:104:si-PCGF5-4アンチセンスヌクレオチド配列
配列ID番号:105:si-HSBP1-1センスヌクレオチド配列
配列ID番号:106:si-HSBP1-1アンチセンスヌクレオチド配列
配列ID番号:107:si-HSBP1-2センスヌクレオチド配列
配列ID番号:108:si-HSBP1-2アンチセンスヌクレオチド配列
配列ID番号:109:si-HSBP1-3センスヌクレオチド配列
配列ID番号:110:si-HSBP1-3アンチセンスヌクレオチド配列
配列ID番号:111:si-HSBP1-4センスヌクレオチド配列
配列ID番号:112:si-HSBP1-4アンチセンスヌクレオチド配列
配列ID番号:113:si-GAPDH-1センスヌクレオチド配列
配列ID番号:114:si-GAPDH-1アンチセンスヌクレオチド配列
配列ID番号:115:si-GAPDH-2センスヌクレオチド配列
配列ID番号:116:si-GAPDH-2アンチセンスヌクレオチド配列
配列ID番号:117:si-GAPDH-3センスヌクレオチド配列
配列ID番号:118:si-GAPDH-3アンチセンスヌクレオチド配列
配列ID番号:119:si-GAPDH-4センスヌクレオチド配列
配列ID番号:120:si-GAPDH-4アンチセンスヌクレオチド配列
配列ID番号:121:si-IFNAR1-1センスヌクレオチド配列
配列ID番号:122:si-IFNAR1-1アンチセンスヌクレオチド配列
配列ID番号:123:si-IFNAR1-2センスヌクレオチド配列
配列ID番号:124:si-IFNAR1-2アンチセンスヌクレオチド配列
配列ID番号:125:si-IFNAR1-3センスヌクレオチド配列
配列ID番号:126:si-IFNAR1-3アンチセンスヌクレオチド配列
配列ID番号:127:si-IFNAR1-4センスヌクレオチド配列
配列ID番号:128:si-IFNAR1-4アンチセンスヌクレオチド配列
配列ID番号:129:si-IL28RA-1センスヌクレオチド配列
配列ID番号:130:si-IL28RA-1アンチセンスヌクレオチド配列
配列ID番号:131:si-IL28RA-2センスヌクレオチド配列
配列ID番号:132:si-IL28RA-2アンチセンスヌクレオチド配列
配列ID番号:133:si-IL28RA-3センスヌクレオチド配列
配列ID番号:134:si-IL28RA-3アンチセンスヌクレオチド配列
配列ID番号:135:si-IL28RA-4センスヌクレオチド配列
配列ID番号:136:si-IL28RA-4アンチセンスヌクレオチド配列
配列ID番号:137:si-MDA5-1センスヌクレオチド配列
配列ID番号:138:si-MDA5-1アンチセンスヌクレオチド配列
配列ID番号:139:si-MDA5-2センスヌクレオチド配列
配列ID番号:140:si-MDA5-2アンチセンスヌクレオチド配列
配列ID番号:141:si-MDA5-3センスヌクレオチド配列
配列ID番号:142:si-MDA5-3アンチセンスヌクレオチド配列
配列ID番号:143:si-MDA5-4センスヌクレオチド配列
配列ID番号:144:si-MDA5-4アンチセンスヌクレオチド配列
配列ID番号:145:si-IL-6-1センスヌクレオチド配列
配列ID番号:146:si-IL-6-1アンチセンスヌクレオチド配列
配列ID番号:147:si-IL-6-2センスヌクレオチド配列
配列ID番号:148:si-IL-6-2アンチセンスヌクレオチド配列
配列ID番号:149:si-IL-6-3センスヌクレオチド配列
配列ID番号:150:si-IL-6-3アンチセンスヌクレオチド配列
配列ID番号:151:si-IL-6-4センスヌクレオチド配列
配列ID番号:152:si-IL-6-4アンチセンスヌクレオチド配列
配列ID番号:153:si-IL1R1-1センスヌクレオチド配列
配列ID番号:154:si-IL1R1-1アンチセンスヌクレオチド配列
配列ID番号:155:si-IL1R1-2センスヌクレオチド配列
配列ID番号:156:si-IL1R1-2アンチセンスヌクレオチド配列
配列ID番号:157:si-IL1R1-3センスヌクレオチド配列
配列ID番号:158:si-IL1R1-3アンチセンスヌクレオチド配列
配列ID番号:159:si-IL1R1-4センスヌクレオチド配列
配列ID番号:160:si-IL1R1-4アンチセンスヌクレオチド配列
配列ID番号:161:ヌクレオチド配列Tol2染色体統合部位
配列ID番号:162:ヌクレオチド配列Tol2染色体統合部位
配列ID番号:163:ヌクレオチド配列Tol2染色体統合部位
配列ID番号:164:ヌクレオチド配列Tol2染色体統合部位
配列ID番号:165:ヌクレオチド配列Tol2染色体統合部位
配列ID番号:166:ヌクレオチド配列Tol2染色体統合部位
配列ID番号:167:ヌクレオチド配列Tol2染色体統合部位
配列ID番号:168:ヌクレオチド配列Tol2染色体統合部位
配列ID番号:169:ヌクレオチド配列Tol2染色体統合部位
Claims (23)
- トランスジェニック鳥類の卵であって、
i)Z染色体上にあってその卵において検出可能なマーカーをコードしている第1の遺伝子改変と;
ii)同じZ染色体上にあって、第2の遺伝子改変が欠けた同質遺伝子型の卵または鳥類と比べたときに、その卵において、および/またはその卵から生まれた鳥類において産生形質を変化させる第2の遺伝子改変を含んでいる、トランスジェニック鳥類の卵。 - 以下の:
i) 前記遺伝子改変が母系遺伝し、
ii) 前記マーカーを、前記卵の殻の完全性を損なうことなく検出することができ、
iii) 前記マーカーが、産卵時点から2日以内に前記卵の殻を破損することなく検出可能であり、
iv) 前記マーカーが、蛍光タンパク質、発光タンパク質、可聴(振動)タンパク質、音響タンパク質、代謝マーカー、又は選択的キレート化タンパク質であり;
v) 前記第1及び/又は第2の遺伝的改変がトランス遺伝子であり;そして
vi) 前記遺伝子改変が、単一の外来遺伝子コンストラクト中に存在する
のうちの1又は複数を含む、請求項1に記載のトランスジェニック鳥類の卵。 - 前記蛍光タンパク質の選択が、緑色蛍光タンパク質(GFP)、増強型緑色蛍光タンパク質(EGFP)、Emerald、Superfolder GFP、Azami Green、mWasabi、TagGFP、TurboGFP、mNeonGreen、mUKG、AcGFP、ZsGreen、Cloverm Sapphire、T-Sapphire、増強型青色蛍光タンパク質(EBFP)、EBFP2、Azurite、TagBFP、mTagBFP、mKalama1、シアン色蛍光タンパク質(CFP)、mCFP、増強型シアン色蛍光タンパク質(ECFP)、mECFP、Cerulean、SCFP3A、mTurquoise、mTurquoise2、CyPet、AmCyan1、Midori-Ishi Cyan、TagCFP、mTFP1(Teal)、黄色蛍光タンパク質(YFP)、増強型黄色蛍光タンパク質(EYFP)、スーパー黄色蛍光タンパク質(SYFP)、Topaz、Venus、Citrine、mCitrine、YPet、TagYFP、TurboYFP、PhiYFP、ZsYellow1、mBanana、Kusabira Orange、Kusabira Orange2、mOrange、mOrange2、dTomato、dTomato-Tandem、赤色蛍光タンパク質(RFP)、TurboRFP、TurboFP602、TurboFP635、Tag ref蛍光タンパク質(RFP)、TagRFP-T、DsRed、DsRed2、DsRed-Express(T1)、DsRed-Monomer、mTangerine、mKeima-Red、mRuby、mRuby2、mApple、mStrawberry、AsRed2、mRFP1、JRed、mCherry、mKate2、mKate(TagFP635)、HcRed1、mRaspberry、dKeima-Tandem、HcRed-Tandem、mPlum、mNeptune、NirFP、Sirius、TagRFP657、AQ143、Kaede、KikGR1、PX-CFP2、mEos2、IrisFP、mEOS3.2、PSmOrange、PAGFP、Dronpa、アロフィコシアニン、GFPuv、R-フィコエリトリン(RPE)、ペリジニンクロロフィル(PerCP)、P3、Katusha、B-フィコエリトリン(BPE)、mKO、J-Redからなされる、請求項2に記載のトランスジェニック鳥類の卵。
- 前記産生形質の選択が、ウイルス産生、組み換えタンパク質産生、筋肉質量、栄養含量、繁殖力からなされる、請求項1~3のいずれか1項に記載のトランスジェニック鳥類の卵。
- 前記産生形質がウイルス産生であり、前記第2の遺伝子改変により、その第2の遺伝子改変が欠けた同質遺伝子型の卵と比べて前記卵において抗ウイルス遺伝子および/または抗ウイルスタンパク質の発現が低下し、その卵が、同質遺伝子型の卵よりも多くのウイルスを産生することができる、請求項4に記載のトランスジェニック鳥類の卵。
- 前記抗ウイルス遺伝子および/または抗ウイルスタンパク質の選択が、IFNAR1、IL-6、CNOT4、MDA5、IFNα、IFNβ、IFNγ、IFNλ、IFNAR2、UBE1DC1、GNAZ、CDX2、LOC100859339、IL28RA、ZFPM2、TRIM50、DNASEIL2、PHF21A、GAPDH、BACE2、HSBP1、PCGF5、IL-1RA、DDI2、CAPN13、UBA5、NPR2、IFIH1、LAMP1、EFR3A、ARRDC3、ABI1、SCAF4、GADL1、ZKSCAN7、PLVAP、RPUSD1、CYYR1、UPF3A、ASAP1、NXF1、TOP1MT、RALGAPB、SUCLA2、GORASP2、NSUN6、CELF1、ANGPTL7、SLC26A6、WBSCR27:siL1、HTT、MYOC、TM9SF2,CEP250、FAM188A、BCAR3、GOLPH3L、HN1、ADCY7、AKAP10、ALX1、CBLN4、CRK、CXORF56、DDX10、EIF2S3、ESF1、GBF1、GCOM1、GTPBP4、HOXB9、IFT43、IMP4、ISY1、KIAA0586、KPNA3、LRRIQ1、LUC7L、MECR、MRPL12、POLR3E、PWP2、RPL7A、SERPINH1、SLC47A2、SMYD2、STAB1、TTK、WNT3、IFNGR1、IFNGR2、IL-10R2、IFNκ、IFNΩ、IL-1RB、XPO1からなされる、請求項5に記載のトランスジェニック鳥類の卵。
- 前記産生形質がウイルス産生であり、前記第2の遺伝子改変により、以下の:
i) その第2の遺伝子改変が欠けた同質遺伝子型の卵と比べて前記卵においてSIAT1遺伝子および/またはSIAT1タンパク質の発現が増加し、その卵が産生するウイルスでは、同質遺伝子型の卵が産生するウイルスと比べて免疫原性が増大すること,
ii)その第2の遺伝子改変が欠けた同質遺伝子型の卵と比べて卵においてα-2,6-結合シアル酸の量が増加するとともにα-2,3-結合シアル酸の量が減少し、その卵が産生するウイルスでは、前記同質遺伝子型の卵が産生するウイルスと比べて免疫原性が増大すること、
iii)その第2の遺伝子改変が欠けた同質遺伝子型の卵と比べて卵において抗微生物タンパク質の発現が増加し、その卵が、前記同質遺伝子型の卵よりも多くのウイルスを産生すること、
のうちの1又は複数を生じる、請求項4に記載のトランスジェニック鳥類の卵。 - 前記鳥類がニワトリである、請求項1~7のいずれか1項に記載のトランスジェニック鳥類の卵。
- 雄である、請求項1~8のいずれか1項に記載のトランスジェニック鳥類の卵。
- トランスジェニック鳥類であって、
i)Z染色体上にあってその鳥類が産んだ卵において検出可能なマーカーをコードしている第1の遺伝子改変と;
ii)同じZ染色体上にあって、第2の遺伝子改変が欠けた同質遺伝子型の卵または鳥類と比べて、その卵において、および/またはその卵から生まれた鳥類において産生形質を変化させる第2の遺伝子改変を、含む鳥類。 - 雌である、請求項10に記載のトランスジェニック鳥類。
- 雄である、請求項10に記載のトランスジェニック鳥類。
- 請求項11に記載のトランスジェニック鳥類を交配することにより産まれ、前記第1の遺伝子改変と、前記第2の遺伝子改変とを有する、鳥類の卵又は子孫。
- 第2の遺伝子改変が欠けた同質遺伝子型の卵と比べてウイルスの産生が増加した雄性卵である、請求項13に記載の鳥類の卵。
- 鳥類の雄性卵を検出する方法であって、
i)請求項11に記載の雌のトランスジェニック鳥類を、前記第1の遺伝子改変が欠けた雄の鳥類と交配させることによって産まれた鳥類の卵を取得し、
ii)その卵をマーカーに関してスクリーニングすることを含んでいて、
前記卵がマーカーを有する場合に雄である、前記方法。 - 工程i)の雄がトランスジェニックではなく、及び/又は前記マーカーが蛍光タンパク質であり、前記卵を第1の波長の光に曝露し、そして第2の波長の光で蛍光を評価することによって、そのマーカーをスクリーニングする、請求項15に記載の方法。
- 鳥類の卵を生産する方法であって、請求項11に記載の雌の鳥類を雄の鳥類と交配させることを含む方法。
- ウイルスを複製する方法であって、
1)請求項1~9のいずれか1項に記載の鳥類の卵を取得し、
2)その卵にウイルスを接種し、そして
3)その卵を所定の期間わたって孵卵してそのウイルスを複製する
ことを含む、前記方法。 - 前記第2の遺伝子改変により、その第2の遺伝子改変が欠けた同質遺伝子型の卵と比べて前記卵において抗ウイルス遺伝子の発現が低下する、請求項18に記載の方法。
- ワクチン組成物を製造する方法であって、
1)請求項18又は19に記載の方法を利用してウイルスを複製し、
2)複製されたそのウイルスまたはその粒子を前記卵から回収し、
3)回収されたそのウイルスからワクチン組成物を調製する
ことを含む方法。 - 工程2)または工程3)が前記ウイルスの不活化を含む、請求項20に記載の方法。
- トランスジェニック鳥類の卵、またはその卵から生まれる鳥類を作り出す方法であって、その卵または鳥類が、
i)Z染色体上にあって鳥類の卵において検出可能なマーカーをコードしている第1の遺伝子改変と;
ii)同じZ染色体上にあって、第2の遺伝子改変が欠けた同質遺伝子型の卵または鳥類と比べて、その卵において、および/またはその卵から生まれた鳥類において産生形質を変化させる第2の遺伝子改変と、を含んでいて、
この方法が、以下の:
1)前記遺伝子改変に関してヘテロ接合型である雄の鳥類を、Z染色体上に前記遺伝子改変を含む雌の鳥類と交配させて、前記遺伝子改変に関してホモ接合型である雄性卵を産ませること、またはその卵から鳥類を生まれさせることを含み、
2)前記遺伝子改変に関してホモ接合型である雄の鳥類を、前記遺伝子改変が欠けた雌の鳥類と交配させて、Z染色体上に前記遺伝子改変を含む雌性卵を産ませること、またはその卵から鳥類を生まれさせること
3)Z染色体上に前記遺伝子改変を含む雌の鳥類を、前記遺伝子改変が欠けた雄の鳥類と交配させて、前記遺伝子改変に関してヘテロ接合型である雄性卵を産ませること、またはその卵から鳥類を生まれさせることを含み、この交配から産まれた雌性卵、またはこの卵から生まれた鳥類は、前記遺伝子改変を欠いている、及び
4)a)前記遺伝子改変に関してヘテロ接合型である雄の鳥類を、Z染色体上に前記遺伝子改変を含む雌の鳥類と交配させて、前記遺伝子改変に関してヘテロ接合型である雄の鳥類を生まれさせ、
b)工程a)で生まれた雄の鳥類を、前記遺伝子改変が欠けた雌の鳥類と交配させて、Z染色体上に前記遺伝子改変を含む雌の鳥類を生まれさせ、
c)工程b)で生まれた雌の鳥類を、前記遺伝子改変が欠けた雄の鳥類と交配させて、前記遺伝子改変に関してヘテロ接合型である雄性卵を産ませ、またはその卵から鳥類を生まれさせ、この交配から産まれた雌性卵、またはこの卵から生まれた鳥類は、前記遺伝子改変を欠いている、
のうちの1を含む、前記方法。 - 請求項22に記載の方法であって、この方法で作り出した前記雌の鳥類を卵産業で利用し、この方法によって作り出した雄性卵をワクチン産業で利用する方法。
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