JP2015514404A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2015514404A5 JP2015514404A5 JP2015506048A JP2015506048A JP2015514404A5 JP 2015514404 A5 JP2015514404 A5 JP 2015514404A5 JP 2015506048 A JP2015506048 A JP 2015506048A JP 2015506048 A JP2015506048 A JP 2015506048A JP 2015514404 A5 JP2015514404 A5 JP 2015514404A5
- Authority
- JP
- Japan
- Prior art keywords
- polynucleotide
- birds
- embryo
- germ cells
- transfection reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000271566 Aves Species 0.000 claims 15
- 229920000023 polynucleotide Polymers 0.000 claims 12
- 239000002157 polynucleotide Substances 0.000 claims 12
- 210000001161 Embryo, Mammalian Anatomy 0.000 claims 10
- 210000004602 germ cell Anatomy 0.000 claims 9
- 239000012096 transfection reagent Substances 0.000 claims 7
- 239000000203 mixture Substances 0.000 claims 5
- 238000001890 transfection Methods 0.000 claims 4
- 229920000160 (ribonucleotides)n+m Polymers 0.000 claims 3
- 210000004204 Blood Vessels Anatomy 0.000 claims 3
- PFNFFQXMRSDOHW-UHFFFAOYSA-N Spermine Natural products NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 claims 3
- 230000023298 conjugation with cellular fusion Effects 0.000 claims 3
- 230000013011 mating Effects 0.000 claims 3
- 230000000051 modifying Effects 0.000 claims 3
- 230000021037 unidirectional conjugation Effects 0.000 claims 3
- 125000002091 cationic group Chemical group 0.000 claims 2
- -1 cationic lipid Chemical class 0.000 claims 2
- 210000004027 cells Anatomy 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 229940063675 spermine Drugs 0.000 claims 2
- 230000029812 viral genome replication Effects 0.000 claims 2
- LDGWQMRUWMSZIU-UHFFFAOYSA-M 2,3-bis(octadec-9-enoxy)propyl-trimethylazanium;chloride Chemical compound [Cl-].CCCCCCCCC=CCCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCCC=CCCCCCCCC LDGWQMRUWMSZIU-UHFFFAOYSA-M 0.000 claims 1
- LDGWQMRUWMSZIU-LQDDAWAPSA-M 2,3-bis[(Z)-octadec-9-enoxy]propyl-trimethylazanium;chloride Chemical group [Cl-].CCCCCCCC\C=C/CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC\C=C/CCCCCCCC LDGWQMRUWMSZIU-LQDDAWAPSA-M 0.000 claims 1
- KWVJHCQQUFDPLU-YEUCEMRASA-N 2,3-bis[[(Z)-octadec-9-enoyl]oxy]propyl-trimethylazanium Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(C[N+](C)(C)C)OC(=O)CCCCCCC\C=C/CCCCCCCC KWVJHCQQUFDPLU-YEUCEMRASA-N 0.000 claims 1
- WALUVDCNGPQPOD-UHFFFAOYSA-M 2,3-di(tetradecoxy)propyl-(2-hydroxyethyl)-dimethylazanium;bromide Chemical compound [Br-].CCCCCCCCCCCCCCOCC(C[N+](C)(C)CCO)OCCCCCCCCCCCCCC WALUVDCNGPQPOD-UHFFFAOYSA-M 0.000 claims 1
- 241000272525 Anas platyrhynchos Species 0.000 claims 1
- 241000272814 Anser sp. Species 0.000 claims 1
- RBGCWNFXPQALRC-UHFFFAOYSA-N CN(CCCN(CCCCN(CCC(N(C(CCCCCCCCCCCCCCC)=O)C(CCCCCCCCCCCCCCC)=O)(C(CCCCCCCCCCCCCCC)=O)C(CCCCCCCCCCCCCCC)=O)C)C)C Chemical compound CN(CCCN(CCCCN(CCC(N(C(CCCCCCCCCCCCCCC)=O)C(CCCCCCCCCCCCCCC)=O)(C(CCCCCCCCCCCCCCC)=O)C(CCCCCCCCCCCCCCC)=O)C)C)C RBGCWNFXPQALRC-UHFFFAOYSA-N 0.000 claims 1
- QPYUGOUSOOCGGQ-UHFFFAOYSA-N CN(CCCN(CCCCN(CCC(N(CCCCCCCCCCCC)CCCCCCCCCCCC)(CCCCCCCCCCCC)CCCCCCCCCCCC)C)C)C Chemical compound CN(CCCN(CCCCN(CCC(N(CCCCCCCCCCCC)CCCCCCCCCCCC)(CCCCCCCCCCCC)CCCCCCCCCCCC)C)C)C QPYUGOUSOOCGGQ-UHFFFAOYSA-N 0.000 claims 1
- NFIDGYIHSCPMMK-SDLHUVDUSA-N CN(CCCN(CCCCN(CCC(N(CCCCCCCC\C=C/CCCCCCCC)CCCCCCCC\C=C/CCCCCCCC)(CCCCCCCC\C=C/CCCCCCCC)CCCCCCCC\C=C/CCCCCCCC)C)C)C Chemical compound CN(CCCN(CCCCN(CCC(N(CCCCCCCC\C=C/CCCCCCCC)CCCCCCCC\C=C/CCCCCCCC)(CCCCCCCC\C=C/CCCCCCCC)CCCCCCCC\C=C/CCCCCCCC)C)C)C NFIDGYIHSCPMMK-SDLHUVDUSA-N 0.000 claims 1
- 229920000033 CRISPR Polymers 0.000 claims 1
- 238000010354 CRISPR gene editing Methods 0.000 claims 1
- XULFJDKZVHTRLG-JDVCJPALSA-N DOSPA trifluoroacetate Chemical group [O-]C(=O)C(F)(F)F.CCCCCCCC\C=C/CCCCCCCCOCC(C[N+](C)(C)CCNC(=O)C(CCCNCCCN)NCCCN)OCCCCCCCC\C=C/CCCCCCCC XULFJDKZVHTRLG-JDVCJPALSA-N 0.000 claims 1
- 210000003278 Egg Shell Anatomy 0.000 claims 1
- 241000287828 Gallus gallus Species 0.000 claims 1
- 101700080605 NUC1 Proteins 0.000 claims 1
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 1
- 241000286209 Phasianidae Species 0.000 claims 1
- 108020004459 Small Interfering RNA Proteins 0.000 claims 1
- 229920001891 Small hairpin RNA Polymers 0.000 claims 1
- 238000010459 TALEN Methods 0.000 claims 1
- DTQVDTLACAAQTR-UHFFFAOYSA-M Trifluoroacetate Chemical compound [O-]C(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-M 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 230000000840 anti-viral Effects 0.000 claims 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 claims 1
- UMGXUWVIJIQANV-UHFFFAOYSA-M didecyl(dimethyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCC[N+](C)(C)CCCCCCCCCC UMGXUWVIJIQANV-UHFFFAOYSA-M 0.000 claims 1
- PSLWZOIUBRXAQW-UHFFFAOYSA-M dimethyl(dioctadecyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCCCCCCCCCCCCCCCC PSLWZOIUBRXAQW-UHFFFAOYSA-M 0.000 claims 1
- 235000013601 eggs Nutrition 0.000 claims 1
- 210000002257 embryonic structures Anatomy 0.000 claims 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 230000001264 neutralization Effects 0.000 claims 1
- 101700006494 nucA Proteins 0.000 claims 1
- 125000002811 oleoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])/C([H])=C([H])\C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 1
- 125000001820 oxy group Chemical group [*:1]O[*:2] 0.000 claims 1
- 239000002924 silencing RNA Substances 0.000 claims 1
- 239000004055 small Interfering RNA Substances 0.000 claims 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 1
- 239000011701 zinc Substances 0.000 claims 1
- 229910052725 zinc Inorganic materials 0.000 claims 1
Claims (16)
(i)トランスフェクション試薬と混合したポリヌクレオチドを含むトランスフェクション混合物を鳥類胚の血管に注入する工程
を含み、それによってポリヌクレオチドが鳥類における1個または複数の生殖細胞のゲノムに挿入される方法。 A method for producing a bird containing genetically modified germ cells, comprising:
(i) A method comprising injecting a transfection mixture comprising a polynucleotide mixed with a transfection reagent into a blood vessel of an avian embryo, whereby the polynucleotide is inserted into the genome of one or more germ cells in the bird.
をさらに含む、請求項1に記載の方法。 2. The method of claim 1, further comprising (ii) incubating the embryo at a temperature sufficient for the embryo to develop in the chick.
(ii) トランスフェクション試薬がDOSPA(2,3-ジオレイルオキシ-N-[2(スペルミンカルボキサミド)エチル]-N,N-ジメチル-1-プロパンアミニウムトリフルオロアセテート)、 DOSPER(1,3-ジオレオイルオキシ-2-(6カルボキシスペルミル)-プロピルアミド)、TMTPS(テトラメチルテトラパルミトイルスペルミン)、TMTOS(テトラメチルテトラオレイルスペルミン)、TMTLS(テトラメチルテトララウリルスペルミン)、TMTMS(テトラメチルテトラミリスチルスペルミン)およびTMDOS(テトラメチルジオレイルスペルミン)から選択される1つまたは複数の多価のカチオン性脂質を含む、請求項4に記載の方法。 (i) The transfection reagent is DOTMA (N- [1- (2,3-dioleoyloxy) -propyl] -N, N, N-trimethylammonium chloride), DOTAP (1,2-bis (oleoyl One selected from (oxy) -3-3- (trimethylammonium) propane), DMRIE (1,2-dimyristyloxypropyl-3-dimethyl-hydroxyethylammonium bromide) and DDAB (dimethyldioctadecylammonium bromide) or including the cationic lipids of the plurality of monovalent and / or
(ii) The transfection reagent is DOSPA (2,3-dioleyloxy-N- [2 (sperminecarboxamido) ethyl] -N, N-dimethyl-1-propaneaminium trifluoroacetate), DOSPER (1,3- (Dioleoyloxy-2- (6carboxyspermyl) -propylamide), TMPTS (tetramethyltetrapalmitoylspermine), TMTOS (tetramethyltetraoleylspermine), TMTLS (tetramethyltetralaurylspermine), TTMMS (tetramethyltetramine) 5. The method of claim 4, comprising one or more polyvalent cationic lipids selected from myristyl spermine) and TMDOS (tetramethyldiolyl spermine) .
(i)トランスフェクション試薬と混合したポリヌクレオチドを含むトランスフェクション混合物を卵内に含有される鳥類胚の血管に注入する工程、および
(ii)胚の雛への発生を可能にするために十分な温度で胚をインキュベートする工程
を含み、
ポリヌクレオチドが鳥類における1個または複数の生殖細胞のゲノムに挿入される方法。 A method for genetically modifying germ cells in birds,
(i) injecting a transfection mixture comprising a polynucleotide mixed with a transfection reagent into a blood vessel of an avian embryo contained in an egg; and
(ii) incubating the embryo at a temperature sufficient to allow development of the embryo into chicks,
A method wherein a polynucleotide is inserted into the genome of one or more germ cells in birds.
(i)請求項1から10のいずれか一項に記載の方法を用いて作製された、遺伝子改変生殖細胞を含む鳥類を得る工程、
(ii)遺伝子改変生殖細胞を含む鳥類から交配により子孫を作製する工程、および
(iii)ゲノムに挿入されたポリヌクレオチドを含む子孫を選択する工程
を含む方法。 A method for producing genetically modified birds,
(i) obtaining a bird comprising a genetically modified germ cell, produced using the method according to any one of claims 1 to 10 ,
(ii) step of producing progeny by mating from birds comprising genetically modified germ cells, and
(iii) A method comprising the step of selecting progeny containing a polynucleotide inserted into the genome.
(i)請求項1から12のいずれか一項に記載の方法を実施して、雛または子孫を作製する工程
(ii)雛または子孫を性的に成熟した鳥類へと育てる工程、および
(iii)性的に成熟した鳥類から交配により遺伝子改変鳥類を作製する工程
を含む方法。 A method of mating genetically modified birds,
(i) carrying out the method according to any one of claims 1 to 12 to produce chicks or offspring
(ii) raising chicks or offspring into sexually mature birds; and
which comprises the step of producing a genetically modified birds by mating from (iii) sexually mature birds.
(i)トランスフェクション試薬と混合したポリヌクレオチドを含むトランスフェクション混合物を鳥類胚の血管に注入し、それによってポリヌクレオチドが鳥類における1個または複数の生殖細胞のゲノムに挿入される工程、および
(ii)胚の雛への発生を可能にするために十分な温度で胚をインキュベートする工程
を含み、ポリヌクレオチドが鳥類における形質をモジュレートするポリペプチドまたは二本鎖領域を含むRNA分子をコードする方法。 A method for modulating traits in birds, comprising:
(i) injecting a transfection mixture comprising a polynucleotide mixed with a transfection reagent into a blood vessel of an avian embryo, whereby the polynucleotide is inserted into the genome of one or more germ cells in the bird; and
(ii) incubating the embryo at a temperature sufficient to allow development into embryonic chicks, wherein the polynucleotide encodes an RNA molecule comprising a polypeptide or double-stranded region that modulates a trait in birds how to.
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261636331P | 2012-04-20 | 2012-04-20 | |
| US61/636,331 | 2012-04-20 | ||
| US201361783823P | 2013-03-14 | 2013-03-14 | |
| US61/783,823 | 2013-03-14 | ||
| AU2013204327A AU2013204327B2 (en) | 2012-04-20 | 2013-04-12 | Cell transfection method |
| AU2013204327 | 2013-04-12 | ||
| PCT/AU2013/000414 WO2013155572A1 (en) | 2012-04-20 | 2013-04-19 | Cell transfection method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015514404A JP2015514404A (en) | 2015-05-21 |
| JP2015514404A5 true JP2015514404A5 (en) | 2016-06-16 |
| JP6531239B2 JP6531239B2 (en) | 2019-06-19 |
Family
ID=49515073
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015506048A Active JP6531239B2 (en) | 2012-04-20 | 2013-04-19 | Cell transfection method |
Country Status (9)
| Country | Link |
|---|---|
| US (4) | US10897881B2 (en) |
| EP (2) | EP3460065B1 (en) |
| JP (1) | JP6531239B2 (en) |
| KR (1) | KR102076784B1 (en) |
| CN (2) | CN112626128A (en) |
| AU (2) | AU2013204327B2 (en) |
| BR (1) | BR112014026186B8 (en) |
| ES (2) | ES2704155T3 (en) |
| WO (1) | WO2013155572A1 (en) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| AU2013204327B2 (en) * | 2012-04-20 | 2016-09-01 | Aviagen | Cell transfection method |
| DK2800811T3 (en) | 2012-05-25 | 2017-07-17 | Univ Vienna | METHODS AND COMPOSITIONS FOR RNA DIRECTIVE TARGET DNA MODIFICATION AND FOR RNA DIRECTIVE MODULATION OF TRANSCRIPTION |
| US10100331B2 (en) | 2012-10-29 | 2018-10-16 | Agency For Science, Technology And Research | Reagent for gene-drug therapeutics |
| KR102243092B1 (en) | 2012-12-06 | 2021-04-22 | 시그마-알드리치 컴퍼니., 엘엘씨 | Crispr-based genome modification and regulation |
| SG11201504523UA (en) | 2012-12-12 | 2015-07-30 | Broad Inst Inc | Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
| WO2014093709A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
| EP2971167B1 (en) | 2013-03-14 | 2019-07-31 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
| KR20160056869A (en) | 2013-06-17 | 2016-05-20 | 더 브로드 인스티튜트, 인코퍼레이티드 | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using viral components |
| WO2014204727A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof |
| EP4245853A3 (en) | 2013-06-17 | 2023-10-18 | The Broad Institute, Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
| CA2915842C (en) | 2013-06-17 | 2022-11-29 | The Broad Institute, Inc. | Delivery and use of the crispr-cas systems, vectors and compositions for hepatic targeting and therapy |
| CN106062197A (en) | 2013-06-17 | 2016-10-26 | 布罗德研究所有限公司 | Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation |
| CN106536729A (en) * | 2013-12-12 | 2017-03-22 | 布罗德研究所有限公司 | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components |
| WO2015089462A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for genome editing |
| CA2932472A1 (en) | 2013-12-12 | 2015-06-18 | Massachusetts Institute Of Technology | Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders |
| JP6793547B2 (en) | 2013-12-12 | 2020-12-02 | ザ・ブロード・インスティテュート・インコーポレイテッド | Optimization Function Systems, methods and compositions for sequence manipulation with the CRISPR-Cas system |
| WO2015089364A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Crystal structure of a crispr-cas system, and uses thereof |
| CN103782953A (en) * | 2014-01-23 | 2014-05-14 | 固镇县玉鹏蔬菜开发有限责任公司 | Gosling plague prevention method |
| CN105265383A (en) * | 2014-10-18 | 2016-01-27 | 颍上县永祥旱粮研究所 | Under forest chicken cultivation method |
| WO2016094867A1 (en) | 2014-12-12 | 2016-06-16 | The Broad Institute Inc. | Protected guide rnas (pgrnas) |
| CN104789599A (en) * | 2015-04-13 | 2015-07-22 | 浙江省农业科学院 | Method for improving transgenic poultry preparation efficiency through lentivirus subgerminal cavity injection |
| CN109536474A (en) | 2015-06-18 | 2019-03-29 | 布罗德研究所有限公司 | Reduce the CRISPR enzyme mutant of undershooting-effect |
| WO2016205759A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| CN108474007A (en) * | 2015-08-07 | 2018-08-31 | 联邦科学技术研究组织 | The method for generating the animal comprising germline modification |
| BR112018010499A8 (en) | 2015-11-24 | 2019-02-26 | Commw Scient Ind Res Org | method for replicating a virus, virus, method for producing a vaccine composition, vaccine composition, in vitro cell population, method for producing a cell population, and cell population |
| EP3380616A4 (en) | 2015-11-24 | 2019-11-06 | Commonwealth Scientific and Industrial Research Organisation | Production of viruses in avian eggs |
| US10858669B2 (en) | 2015-12-25 | 2020-12-08 | National Institute Of Advanced Industrial Science And Technology | Genetically modified chicken egg with an exogenous sequence knocked into the ovalbumin gene |
| MA48783A (en) * | 2017-05-25 | 2020-04-08 | Eggxyt Ltd | SEX DETERMINATION PROCESSES OF AVIAN EMBRYOS IN UNHATCHED UFS AND CORRESPONDING MEANS |
| CN109275622A (en) * | 2017-07-20 | 2019-01-29 | 龚海华 | A kind of cyclic culture method of laying hen |
| CN107723313A (en) * | 2017-10-30 | 2018-02-23 | 扬州大学 | A kind of method that allogenic material imports chicken embryo |
| JP7568507B2 (en) * | 2017-11-15 | 2024-10-16 | ウィヤード・サイエンス・エルエルシー | Methods and compositions for non-myeloablative bone marrow reconstitution - Patents.com |
| KR102103103B1 (en) * | 2018-08-16 | 2020-04-21 | (주)라트바이오 | Transgenic animals and transgenic embryos producing an engineered nuclease |
| JP7418470B2 (en) * | 2019-04-08 | 2024-01-19 | ディーエヌエー ツーポイントオー インク． | Integration of nucleic acid constructs into eukaryotic cells using transposase derived from Orydias |
Family Cites Families (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5162215A (en) * | 1988-09-22 | 1992-11-10 | Amgen Inc. | Method of gene transfer into chickens and other avian species |
| GB8906214D0 (en) | 1989-03-17 | 1989-05-04 | Nat Res Dev | Introducing an exogenous gene into birds |
| US5631329A (en) | 1990-08-27 | 1997-05-20 | Dendritech, Inc. | Process for producing hyper-comb-branched polymers |
| US5453357A (en) | 1992-10-08 | 1995-09-26 | Vanderbilt University | Pluripotential embryonic stem cells and methods of making same |
| US5266106A (en) | 1992-10-22 | 1993-11-30 | Xerox Corporation | Ink compositions with dendrimer grafts |
| WO1996031549A1 (en) | 1995-04-07 | 1996-10-10 | Mueller Egbert | Dendrimeric graft polymers |
| US20030069173A1 (en) | 1998-03-16 | 2003-04-10 | Life Technologies, Inc. | Peptide-enhanced transfections |
| JP4320054B2 (en) * | 1996-08-13 | 2009-08-26 | ノバルティス バクシンズ アンド ダイアグノスティックス，インコーポレーテッド | Compositions and methods for polynucleotide delivery |
| GB9710809D0 (en) | 1997-05-23 | 1997-07-23 | Medical Res Council | Nucleic acid binding proteins |
| CN1195844C (en) | 1997-08-04 | 2005-04-06 | 马萨诸塞州大学 | Avian primordial germ cell (PGC) cell line and method for long term culturing thereof |
| JP2003517261A (en) | 1997-08-04 | 2003-05-27 | ユニバシティ オブ マサチューセッツ、ア パブリック インスチチユーション オブ ハイアー エデュケイション オブ ザ コモンウエルス オブ マサチューセッツ、アズ リプリゼンテッド バイ イッツ アマースト キャンパス | Production of avian embryonic germ (EG) cell lines by long-term culture of PGCs, their use for cloning and chimera formation |
| ES2357495T3 (en) * | 1997-10-16 | 2011-04-27 | University Of Georgia Research Foundation, Inc. | TRANSGENIC BIRDS AND PROTEIN PRODUCTION. |
| DE60037107T2 (en) | 1999-02-11 | 2008-09-25 | Hanmi Pharm. Co., Ltd. | PLURIPOTENTE EMBRYONIC GERMANY FROM BIRDS |
| US6140118A (en) | 1999-08-11 | 2000-10-31 | Wisconsin Alumni Research Foundation | Avian blastodermal cell lines |
| JP4609869B2 (en) | 1999-12-03 | 2011-01-12 | 独立行政法人科学技術振興機構 | Transposon transferase and gene modification method |
| US8202979B2 (en) | 2002-02-20 | 2012-06-19 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid |
| JP4300287B2 (en) | 2002-02-14 | 2009-07-22 | 国立大学法人東京海洋大学 | Induction of germ line differentiation by transplantation of isolated primordial germ cells |
| JP4364474B2 (en) | 2002-02-15 | 2009-11-18 | 大学共同利用機関法人情報・システム研究機構 | Functional transposons in mammals |
| US20040172667A1 (en) * | 2002-06-26 | 2004-09-02 | Cooper Richard K. | Administration of transposon-based vectors to reproductive organs |
| US7527966B2 (en) * | 2002-06-26 | 2009-05-05 | Transgenrx, Inc. | Gene regulation in transgenic animals using a transposon-based vector |
| KR100502889B1 (en) | 2003-04-21 | 2005-07-20 | 한미약품(주) | Method for Enhancing Germline Transmission Efficiency of Avian Primordial Germ Cells |
| EP1850659B1 (en) | 2005-02-01 | 2016-11-23 | Synageva BioPharma Corp. | Long term culture of chicken primordial germ cells |
| RU2533804C2 (en) | 2005-03-31 | 2014-11-20 | Ронен КАХАНА | Producing poultry and other animals resistant to viral diseases |
| US20070293449A1 (en) * | 2006-06-20 | 2007-12-20 | Nastech Pharmaceutical Company Inc. | Compositions and methods for delivery of double-stranded rna |
| AU2007319881B2 (en) * | 2006-11-13 | 2012-05-17 | Sangamo Therapeutics, Inc. | Zinc finger nuclease for targeting the human glucocorticoid receptor locus |
| WO2008138072A1 (en) | 2007-05-16 | 2008-11-20 | Mat Malta Advanced Technologies Limited | Treatment and prevention of influenza |
| WO2009023800A1 (en) * | 2007-08-14 | 2009-02-19 | Origen Therapeutics | Transgenic chickens with an inactivated endogenous gene locus |
| NZ583783A (en) * | 2007-09-20 | 2012-10-26 | Commw Scient Ind Res Org | Avian interferon-lambda polypeptide and genetic sequences encoding the same |
| US20110041195A1 (en) * | 2009-08-11 | 2011-02-17 | Sangamo Biosciences, Inc. | Organisms homozygous for targeted modification |
| KR20140046408A (en) * | 2011-02-25 | 2014-04-18 | 리컴비네틱스 인코포레이티드 | Genetically modified animals and methods for making the same |
| AU2013204327B2 (en) * | 2012-04-20 | 2016-09-01 | Aviagen | Cell transfection method |
| WO2014138792A1 (en) * | 2013-03-14 | 2014-09-18 | Commonwealth Scientific And Industrial Research Organisation | Double-stranded rna |
| WO2014165825A2 (en) * | 2013-04-04 | 2014-10-09 | President And Fellows Of Harvard College | Therapeutic uses of genome editing with crispr/cas systems |
| ES2965286T3 (en) * | 2014-07-14 | 2024-04-12 | Univ Washington State | NANOS silencing that kills germ cells |
| CN108474007A (en) * | 2015-08-07 | 2018-08-31 | 联邦科学技术研究组织 | The method for generating the animal comprising germline modification |
| CN105524941A (en) * | 2016-01-19 | 2016-04-27 | 扬州大学 | Gene transfer system and method for specific poultry primordial germ cell |
| TW202241259A (en) * | 2020-12-31 | 2022-11-01 | 以色列國家農業部、農村發展農業研究組織（沃爾卡尼研究所） | Sterile avian embryos, production and uses thereof |
-
2013
- 2013-04-12 AU AU2013204327A patent/AU2013204327B2/en active Active
- 2013-04-19 WO PCT/AU2013/000414 patent/WO2013155572A1/en active Application Filing
- 2013-04-19 EP EP18187574.1A patent/EP3460065B1/en active Active
- 2013-04-19 CN CN202011320159.3A patent/CN112626128A/en active Pending
- 2013-04-19 CN CN201380031734.XA patent/CN104619848B/en active Active
- 2013-04-19 US US14/394,712 patent/US10897881B2/en active Active
- 2013-04-19 KR KR1020147031173A patent/KR102076784B1/en active IP Right Grant
- 2013-04-19 JP JP2015506048A patent/JP6531239B2/en active Active
- 2013-04-19 EP EP13777737.1A patent/EP2839016B1/en active Active
- 2013-04-19 ES ES13777737T patent/ES2704155T3/en active Active
- 2013-04-19 AU AU2013248945A patent/AU2013248945A1/en not_active Abandoned
- 2013-04-19 BR BR112014026186A patent/BR112014026186B8/en active IP Right Grant
- 2013-04-19 ES ES18187574T patent/ES2963732T3/en active Active
-
2019
- 2019-04-15 US US16/384,782 patent/US11369096B2/en active Active
-
2020
- 2020-12-23 US US17/133,142 patent/US20210112789A1/en active Pending
-
2022
- 2022-06-27 US US17/809,219 patent/US20230189769A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2015514404A5 (en) | ||
| ES2704155T3 (en) | Cell transfection method | |
| RU2011129621A (en) | WAYS OF MODULATION OF BIRD FLOOD | |
| Sifuentes-Romero et al. | Post-transcriptional gene silencing by RNA interference in non-mammalian vertebrate systems: where do we stand? | |
| JP2016506734A (en) | Cell lines for virus production and methods of use thereof | |
| JP2012516689A5 (en) | ||
| JP2012125256A (en) | Creating poultry and other animals resistant to viral disease | |
| CN108884461A (en) | Virus is generated in birds, beasts and eggs | |
| EA017948B1 (en) | Treatment and prevention of influenza | |
| MX2016006315A (en) | Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos. | |
| Dai et al. | Infectivity and pathogenicity of Newcastle disease virus strains of different avian origin and different virulence for mallard ducklings | |
| Bradford et al. | RNA interference-based technology: what role in animal agriculture? | |
| US20140289881A1 (en) | Double-stranded rna | |
| KR102131883B1 (en) | Animal model of cre-dependent anti-depressant phenotype and manufacturing method thereof | |
| CN106337049A (en) | Breeding method of anti-influenza transgenic animals expressing double genes | |
| Matsubara et al. | Detection of the EGFP sequence in breast muscle of 3‐year‐old chicken after transfection using sonoporation | |
| Vasconi et al. | Effect of temperature on fatty acid composition of unfed Siberian sturgeon (A. baerii) larvae | |
| RU2021113247A (en) | GENETICALLY MODIFIED STERILE BIRDS AND METHOD OF THEIR REPRODUCTION | |
| Tizard et al. | Precision genome engineering in the chicken: The gap between science and market place | |
| LI | Establishment and identification of transgenic breast cancer mouse model with Her2 overexpression | |
| Vetokh et al. | The influence of lentiviral vectors with inserts of varying length on the embryonic development and hatching of transgenic poultry | |
| Stewart et al. | RNAi for Viral Disease Control | |
| Callaway | Male ruffs look and behave differently around females, depending on their version of a 125-gene stretch of DNA. | |
| Eswaran | Isolation, Identification and Molecular Characterization of Avian Metapneumovirus (aMPV) in Chicken | |
| Tiley et al. | GM and disease resistance-inhibition of influenza virus in transgenic chickens. |