JP2015053881A - 熱対流生成用チップ、熱対流生成装置、及び熱対流生成方法 - Google Patents
熱対流生成用チップ、熱対流生成装置、及び熱対流生成方法 Download PDFInfo
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Abstract
【解決手段】液体を加熱又は冷却すると同時に液体に遠心力を付与することにより液体の熱対流を生じさせる熱対流生成装置1であって、ディスク状基板11と、ディスク状基板における軸線AXと直交する面内に形成された熱対流用流路14とを含み、ディスク状基板の軸線周りに回転させることにより熱対流用流路内の液体に遠心力を付与するようにした熱対流生成用チップ10を装脱可能なステージ20と、熱対流用流路の一部を加熱又は冷却する熱源30と、熱対流生成用チップを軸線周りに回転駆動するモータ40と、熱源及びモータを制御する制御手段50とを含む。
【選択図】 図2
Description
10 熱対流生成用チップ
14 熱対流用流路
15 液体供給路
16 ガス排出路
20 ステージ(チップ装着部)
30 熱源
31 第1ヒータ(低温熱源)
31b 加熱部
32 第2ヒータ(高温熱源)
32b 加熱部
40 モータ(駆動手段)
50 制御手段
60 ヒートシンク(放熱手段)
91 励起光光源
92 蛍光検出器
93 検知光光源(位置検知手段)
94 検知光検出器(位置検知手段)
95 被検知部(位置検知手段)
96 演算制御部(位置検知手段、光源制御手段)
100 制御手段
S 空隙
E1 低温エリア
E2 空隙エリア
E3 高温エリア
Claims (21)
- 少なくとも一つの環状の熱対流用流路を含み、回転させることにより前記熱対流用流路内の液体に遠心力を付与する、熱対流生成用チップ。
- 前記熱対流用流路内に液体を供給するための液体供給路を少なくとも1つ設けた、請求項1に記載の熱対流生成用チップ。
- 回転させることにより前記液体供給路内の液体が遠心力によって前記熱対流用流路内に進入する、請求項2に記載の熱対流生成用チップ。
- 前記熱対流用流路内の液体中に含まれるガスを受け入れるガス排出路を少なくとも1つ設けた、請求項1から請求項3のうちの1項に記載の熱対流生成用チップ。
- 前記熱対流用流路が真円状である、請求項1から請求項4のうちの1項に記載の熱対流生成用チップ。
- 軸線周りに回転するとともに、前記軸線に対して対称的に配置された複数の前記熱対流用流路を備える、請求項1から請求項5のうちの1項に記載の熱対流生成用チップ。
- 前記熱対流用流路の壁面の表面粗さRaが100nm以下である、請求項1から請求項6のうちの1項に記載の熱対流生成用チップ。
- 前記熱対流用流路の壁面の材質が、環状オレフィン、ポリプロピレン、及びポリカーボネートのうちのいずれかである、請求項1から請求項7のうちの1項に記載の熱対流生成用チップ。
- 前記熱対流用流路内で熱対流PCRを行う、請求項1から請求項8のうちの1項に記載の熱対流生成用チップ。
- 少なくとも一つの熱対流用流路を含み、回転させることにより前記熱対流用流路内の液体に遠心力を付与する熱対流生成用チップを装脱可能なチップ装着部と、
前記熱対流用流路の一部を加熱又は冷却する熱源と、
前記熱対流生成用チップを回転駆動する駆動手段と
を含む、熱対流生成装置。
- 前記熱源は、低温熱源と高温熱源とを含んでおり、
前記低温熱源の加熱部と前記高温熱源の加熱部との温度差が10℃以上であり、
前記低温熱源の前記加熱部と前記高温熱源の前記加熱部との間の空隙と前記低温熱源の前記加熱部との温度差が10℃以上であるとともに、前記空隙と前記高温熱源の前記加熱部との温度差が10℃以上である、請求項10に記載の熱対流生成装置。
- 前記熱対流用流路のうち、前記熱源に対向するエリアの温度が、前記熱対流用流路のうち、前記エリア以外のエリアの温度よりも大きいか又は小さい、請求項10又は請求項11に記載の熱対流生成装置。
- 前記熱源による前記熱対流用流路の加熱温度又は冷却温度、前記駆動手段による前記熱対流生成用チップの回転駆動速度及び回転駆動時間を制御する制御手段を備える、請求項10から請求項12のうちの1項に記載の熱対流生成装置。
- 前記熱対流用流路内で熱対流PCRを行う、請求項10から請求項13のうちの1項に記載の熱対流生成装置。
- 前記熱対流用流路内の前記液体に含まれる蛍光色素を励起する励起光を前記熱対流用流路内の前記液体に照射する励起光光源と、
前記蛍光色素に前記励起光を照射することにより前記蛍光色素によって放出される蛍光を検出する蛍光検出器と、
前記蛍光検出器によって検出された蛍光に基づいてDNAの増幅量を算出する演算制御部と
を含む、請求項14に記載の熱対流生成装置。
- 前記熱対流生成用チップの回転駆動中における前記熱対流用流路の位置を検知する位置検知手段と、
前記位置検知手段の検知結果に基づいて前記励起光光源を駆動する光源制御手段と
をさらに含む、請求項15に記載の熱対流生成装置。
- 前記励起光光源及び前記蛍光検出器が前記熱対流生成用チップと一体的に回転する、請求項15に記載の熱対流生成装置。
- 少なくとも一つの環状の熱対流用流路を含み、回転させることにより前記熱対流用流路内の液体に遠心力を付与する熱対流生成用チップを準備するチップ準備工程と、
前記熱対流用流路内に前記液体を供給する液体供給工程と、
前記熱対流生成用チップを回転駆動して前記液体に遠心力を付与しつつ前記熱対流用流路の一部を加熱又は冷却する熱対流促進工程と
を含む、熱対流生成方法。
- 前記液体は、前記液体を加熱する熱源の最高温度よりも沸点が高い及び/又は前記液体よりも比重が小さい蒸発抑制用液体を含む、請求項18に記載の熱対流生成方法。
- 前記熱対流用流路内で熱対流PCRを行う、請求項18又は請求項19に記載の熱対流生成方法。
- 前記液体が蛍光色素を含み、
前記熱対流促進工程において、前記蛍光色素を励起する励起光を前記熱対流用流路内の前記液体に照射する励起光照射工程と、
前記励起光が照射された前記蛍光色素によって放出される蛍光を検出する蛍光検出工程と、
前記検出された蛍光に基づいてDNAの増幅量を算出するDNA増幅量算出工程とを実行する、請求項20に記載の熱対流生成方法。
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