JP2015037412A5 - - Google Patents
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- Publication number
- JP2015037412A5 JP2015037412A5 JP2014195515A JP2014195515A JP2015037412A5 JP 2015037412 A5 JP2015037412 A5 JP 2015037412A5 JP 2014195515 A JP2014195515 A JP 2014195515A JP 2014195515 A JP2014195515 A JP 2014195515A JP 2015037412 A5 JP2015037412 A5 JP 2015037412A5
- Authority
- JP
- Japan
- Prior art keywords
- expression vector
- gene
- foreign gene
- transformant
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108090000623 proteins and genes Proteins 0.000 claims 52
- 239000013604 expression vector Substances 0.000 claims 27
- 238000004519 manufacturing process Methods 0.000 claims 13
- 235000018102 proteins Nutrition 0.000 claims 13
- 102000004169 proteins and genes Human genes 0.000 claims 13
- 210000004027 cell Anatomy 0.000 claims 10
- 206010059866 Drug resistance Diseases 0.000 claims 9
- 238000012258 culturing Methods 0.000 claims 9
- 230000001131 transforming effect Effects 0.000 claims 9
- 238000000034 method Methods 0.000 claims 8
- 229930189065 blasticidin Natural products 0.000 claims 5
- 108020004705 Codon Proteins 0.000 claims 4
- 108010084455 Zeocin Proteins 0.000 claims 4
- 230000001771 impaired effect Effects 0.000 claims 4
- CWCMIVBLVUHDHK-ZSNHEYEWSA-N phleomycin D1 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC[C@@H](N=1)C=1SC=C(N=1)C(=O)NCCCCNC(N)=N)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C CWCMIVBLVUHDHK-ZSNHEYEWSA-N 0.000 claims 4
- 241001465754 Metazoa Species 0.000 claims 3
- 229930193140 Neomycin Natural products 0.000 claims 3
- 239000000654 additive Substances 0.000 claims 3
- 230000000996 additive effect Effects 0.000 claims 3
- 229960004927 neomycin Drugs 0.000 claims 3
- 238000012216 screening Methods 0.000 claims 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 2
- 239000004475 Arginine Substances 0.000 claims 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims 1
- 239000004471 Glycine Substances 0.000 claims 1
- 108010025815 Kanamycin Kinase Proteins 0.000 claims 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims 1
- 239000004472 Lysine Substances 0.000 claims 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 1
- 241000124008 Mammalia Species 0.000 claims 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims 1
- 239000004473 Threonine Substances 0.000 claims 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims 1
- 235000004279 alanine Nutrition 0.000 claims 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 1
- 235000009582 asparagine Nutrition 0.000 claims 1
- 229960001230 asparagine Drugs 0.000 claims 1
- 235000003704 aspartic acid Nutrition 0.000 claims 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims 1
- 238000010367 cloning Methods 0.000 claims 1
- 235000018417 cysteine Nutrition 0.000 claims 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 1
- 208000035475 disorder Diseases 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 239000003623 enhancer Substances 0.000 claims 1
- 235000013922 glutamic acid Nutrition 0.000 claims 1
- 239000004220 glutamic acid Substances 0.000 claims 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims 1
- 230000010354 integration Effects 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims 1
- 230000008488 polyadenylation Effects 0.000 claims 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims 1
- 239000004474 valine Substances 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014195515A JP6022518B2 (ja) | 2008-02-27 | 2014-09-25 | 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008046782 | 2008-02-27 | ||
| JP2008046782 | 2008-02-27 | ||
| JP2014195515A JP6022518B2 (ja) | 2008-02-27 | 2014-09-25 | 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010500766A Division JP5648912B2 (ja) | 2008-02-27 | 2009-02-27 | 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015037412A JP2015037412A (ja) | 2015-02-26 |
| JP2015037412A5 true JP2015037412A5 (https=) | 2015-07-02 |
| JP6022518B2 JP6022518B2 (ja) | 2016-11-09 |
Family
ID=41016160
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010500766A Expired - Fee Related JP5648912B2 (ja) | 2008-02-27 | 2009-02-27 | 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 |
| JP2014195515A Expired - Fee Related JP6022518B2 (ja) | 2008-02-27 | 2014-09-25 | 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010500766A Expired - Fee Related JP5648912B2 (ja) | 2008-02-27 | 2009-02-27 | 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US8653249B2 (https=) |
| EP (1) | EP2258843B1 (https=) |
| JP (2) | JP5648912B2 (https=) |
| KR (1) | KR101604502B1 (https=) |
| CN (1) | CN102016025B (https=) |
| AU (1) | AU2009218069B2 (https=) |
| CA (1) | CA2716766A1 (https=) |
| DK (1) | DK2258843T3 (https=) |
| ES (1) | ES2616021T3 (https=) |
| PT (1) | PT2258843T (https=) |
| WO (1) | WO2009107775A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2380979T3 (da) | 2008-12-22 | 2015-06-22 | Univ Hokkaido Nat Univ Corp | Proteinstof med trippelhelixstruktur samt fremgangsmåde til dets fremstilling |
| CN102325881B (zh) * | 2008-12-22 | 2014-08-20 | 国立大学法人北海道大学 | 用于用动物细胞大量生产源自外源基因的蛋白质的表达载体及其应用 |
| WO2016130583A1 (en) * | 2015-02-13 | 2016-08-18 | The Board Of Trustees Of The Leland Stanford Junior University | Non-silencing selectable marker genes and methods of use |
| JP7416355B2 (ja) * | 2021-03-15 | 2024-01-17 | 株式会社ジー・キューブ | 選択マーカー発現カセット |
| CN113969284B (zh) * | 2021-11-23 | 2022-07-12 | 江南大学 | 一种cho细胞基因nw_003614889.1内稳定表达蛋白质的位点及其应用 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU572108B2 (en) | 1983-01-19 | 1988-05-05 | Genentech Inc. | Human tpa production using vectors coding for dhfr protein |
| NZ210501A (en) | 1983-12-13 | 1991-08-27 | Kirin Amgen Inc | Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence |
| JP2648860B2 (ja) | 1986-05-08 | 1997-09-03 | イエダ リサーチ アンド デベロップメント コンパニー リミテッド | ヒトインターフエロン−β２Ａ及びヒトインターフエロン−β２Ｂ、該インターフエロンをコードする遺伝子を含むベクター、該インターフエロンを産生するセルライン及び該インターフエロンの医薬品としての用途 |
| DE4142077A1 (de) | 1991-12-19 | 1993-06-24 | Boehringer Mannheim Gmbh | Verfahren zur expression rekombinanter antikoerper |
| JPH0630788A (ja) | 1992-07-16 | 1994-02-08 | Otsuka Pharmaceut Co Ltd | ヒトインターロイキン−１に対する組換え抗体 |
| JP3919830B2 (ja) | 1992-11-28 | 2007-05-30 | 財団法人化学及血清療法研究所 | 抗ネコヘルペスウイルス−１組換え抗体および該抗体をコードする遺伝子断片 |
| JP3582965B2 (ja) | 1996-08-23 | 2004-10-27 | 株式会社イムノ・ジャパン | 哺乳動物細胞用発現ベクター |
| WO2004070030A1 (ja) | 2003-02-03 | 2004-08-19 | Immuno Japan Inc. | 動物細胞用高発現ベクター |
| ES2384391T3 (es) * | 2004-11-08 | 2012-07-04 | Chromagenics B.V. | Selección de células hospedantes que expresan proteína a altos niveles |
| KR101271884B1 (ko) | 2004-11-08 | 2013-06-05 | 크로마제닉스 비.브이. | 고수준으로 단백질을 발현하는 숙주세포의 선택 |
| CN100381573C (zh) * | 2005-04-14 | 2008-04-16 | 北京天广实生物技术有限公司 | 快速构建目标基因高表达的哺乳动物细胞株的体系和方法 |
-
2009
- 2009-02-27 EP EP09715053.6A patent/EP2258843B1/en active Active
- 2009-02-27 US US12/918,776 patent/US8653249B2/en not_active Expired - Fee Related
- 2009-02-27 CN CN200980114565.XA patent/CN102016025B/zh not_active Expired - Fee Related
- 2009-02-27 ES ES09715053.6T patent/ES2616021T3/es active Active
- 2009-02-27 KR KR1020107021057A patent/KR101604502B1/ko not_active Expired - Fee Related
- 2009-02-27 JP JP2010500766A patent/JP5648912B2/ja not_active Expired - Fee Related
- 2009-02-27 WO PCT/JP2009/053682 patent/WO2009107775A1/ja not_active Ceased
- 2009-02-27 DK DK09715053.6T patent/DK2258843T3/en active
- 2009-02-27 AU AU2009218069A patent/AU2009218069B2/en not_active Ceased
- 2009-02-27 PT PT97150536T patent/PT2258843T/pt unknown
- 2009-02-27 CA CA2716766A patent/CA2716766A1/en not_active Abandoned
-
2014
- 2014-09-25 JP JP2014195515A patent/JP6022518B2/ja not_active Expired - Fee Related
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