JP2014534812A5 - - Google Patents
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- JP2014534812A5 JP2014534812A5 JP2014536328A JP2014536328A JP2014534812A5 JP 2014534812 A5 JP2014534812 A5 JP 2014534812A5 JP 2014536328 A JP2014536328 A JP 2014536328A JP 2014536328 A JP2014536328 A JP 2014536328A JP 2014534812 A5 JP2014534812 A5 JP 2014534812A5
- Authority
- JP
- Japan
- Prior art keywords
- target polynucleotide
- pore
- helicase
- characterizing
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002157 polynucleotide Substances 0.000 claims 26
- 102000040430 polynucleotide Human genes 0.000 claims 26
- 108091033319 polynucleotide Proteins 0.000 claims 26
- 239000011148 porous material Substances 0.000 claims 23
- 108060004795 Methyltransferase Proteins 0.000 claims 18
- 238000000034 method Methods 0.000 claims 13
- 102100022536 Helicase POLQ-like Human genes 0.000 claims 10
- 101000899334 Homo sapiens Helicase POLQ-like Proteins 0.000 claims 10
- 238000005259 measurement Methods 0.000 claims 5
- 108010013381 Porins Proteins 0.000 claims 4
- 150000001413 amino acids Chemical class 0.000 claims 4
- 102000007739 porin activity proteins Human genes 0.000 claims 4
- 230000000694 effects Effects 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 108091005703 transmembrane proteins Proteins 0.000 claims 3
- 102000035160 transmembrane proteins Human genes 0.000 claims 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- 108010006464 Hemolysin Proteins Proteins 0.000 claims 2
- 108010014603 Leukocidins Proteins 0.000 claims 2
- 241000187480 Mycobacterium smegmatis Species 0.000 claims 2
- 101710203388 Outer membrane porin G Proteins 0.000 claims 2
- 238000012512 characterization method Methods 0.000 claims 2
- 239000012530 fluid Substances 0.000 claims 2
- 239000003228 hemolysin Substances 0.000 claims 2
- 230000003993 interaction Effects 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- 102000004895 Lipoproteins Human genes 0.000 claims 1
- 108090001030 Lipoproteins Proteins 0.000 claims 1
- 241000588653 Neisseria Species 0.000 claims 1
- 108700006385 OmpF Proteins 0.000 claims 1
- 108010073429 Type V Secretion Systems Proteins 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 125000000539 amino acid group Chemical group 0.000 claims 1
- 230000005669 field effect Effects 0.000 claims 1
- 238000002847 impedance measurement Methods 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 230000011987 methylation Effects 0.000 claims 1
- 238000007069 methylation reaction Methods 0.000 claims 1
- 230000003287 optical effect Effects 0.000 claims 1
- 108010014203 outer membrane phospholipase A Proteins 0.000 claims 1
- 230000003647 oxidation Effects 0.000 claims 1
- 238000007254 oxidation reaction Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 125000006850 spacer group Chemical group 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161549998P | 2011-10-21 | 2011-10-21 | |
| US61/549,998 | 2011-10-21 | ||
| US201261599244P | 2012-02-15 | 2012-02-15 | |
| US61/599,244 | 2012-02-15 | ||
| PCT/GB2012/052579 WO2013057495A2 (en) | 2011-10-21 | 2012-10-18 | Enzyme method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014534812A JP2014534812A (ja) | 2014-12-25 |
| JP2014534812A5 true JP2014534812A5 (OSRAM) | 2015-12-10 |
| JP6226869B2 JP6226869B2 (ja) | 2017-11-08 |
Family
ID=47073477
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014536328A Active JP6226869B2 (ja) | 2011-10-21 | 2012-10-18 | 酵素法 |
Country Status (9)
| Country | Link |
|---|---|
| US (4) | US9758823B2 (OSRAM) |
| EP (2) | EP2987870B1 (OSRAM) |
| JP (1) | JP6226869B2 (OSRAM) |
| KR (1) | KR20140090633A (OSRAM) |
| CN (1) | CN104039979B (OSRAM) |
| AU (1) | AU2012324639B2 (OSRAM) |
| BR (1) | BR112014009579B1 (OSRAM) |
| CA (1) | CA2852812A1 (OSRAM) |
| WO (1) | WO2013057495A2 (OSRAM) |
Families Citing this family (143)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2008217579A1 (en) | 2007-02-20 | 2008-08-28 | Oxford Nanopore Technologies Limited | Formation of lipid bilayers |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| WO2010034018A2 (en) | 2008-09-22 | 2010-03-25 | University Of Washington | Msp nanopores and related methods |
| WO2010086622A1 (en) | 2009-01-30 | 2010-08-05 | Oxford Nanopore Technologies Limited | Adaptors for nucleic acid constructs in transmembrane sequencing |
| AU2012264497B2 (en) | 2011-05-27 | 2017-06-15 | Oxford Nanopore Technologies Limited | Coupling method |
| WO2013014451A1 (en) | 2011-07-25 | 2013-01-31 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
| CA3113287C (en) | 2011-09-23 | 2022-12-20 | Oxford Nanopore Technologies Limited | Analysis of a polymer comprising polymer units |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| WO2013098562A2 (en) | 2011-12-29 | 2013-07-04 | Oxford Nanopore Technologies Limited | Enzyme method |
| US9617591B2 (en) | 2011-12-29 | 2017-04-11 | Oxford Nanopore Technologies Ltd. | Method for characterising a polynucleotide by using a XPD helicase |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| US10739341B2 (en) | 2012-02-15 | 2020-08-11 | Oxford Nanopore Technologies Limited | Aptamer method |
| US20150057948A1 (en) | 2012-02-16 | 2015-02-26 | Oxford Nanopore Technologies Limited | Analysis of measurements of a polymer |
| KR102083695B1 (ko) | 2012-04-10 | 2020-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 리세닌 기공 |
| EP2875152B1 (en) | 2012-07-19 | 2019-10-09 | Oxford Nanopore Technologies Limited | Enzyme construct |
| JP6614972B2 (ja) | 2012-07-19 | 2019-12-04 | オックスフォード ナノポール テクノロジーズ リミテッド | 修飾ヘリカーゼ |
| WO2014013259A1 (en) | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Ssb method |
| EP2895618B1 (en) | 2012-09-14 | 2017-07-26 | Oxford Nanopore Technologies Limited | Sample preparation method |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| WO2014072703A1 (en) | 2012-11-06 | 2014-05-15 | Oxford Nanopore Technologies Limited | Quadruplex method |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| SG11201507138RA (en) | 2013-03-08 | 2015-10-29 | Oxford Nanopore Tech Ltd | Enzyme stalling method |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| WO2014190299A2 (en) * | 2013-05-23 | 2014-11-27 | Arizona Board Of Regents Acting For And On Behalf Of Arizona State University | Chemistry, systems and methods of translocation of a polymer through a nanopore |
| GB201406151D0 (en) * | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| US10724018B2 (en) | 2013-10-18 | 2020-07-28 | Oxford Nanopore Technologies Ltd. | Modified helicases |
| CN105934522B (zh) | 2013-11-26 | 2021-07-20 | 伊鲁米那股份有限公司 | 用于多核苷酸测序的组合物和方法 |
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| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| US10359395B2 (en) | 2014-02-19 | 2019-07-23 | University Of Washington | Nanopore-based analysis of protein characteristics |
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| CN106460061B (zh) | 2014-04-04 | 2020-03-06 | 牛津纳米孔技术公司 | 在双链核酸分子的两端使用纳米孔和锚分子来表征所述双链核酸的方法 |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| WO2015166275A1 (en) | 2014-05-02 | 2015-11-05 | Oxford Nanopore Technologies Limited | Mutant pores |
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| GB201411285D0 (en) * | 2014-06-25 | 2014-08-06 | Prosser Joseph | Sequencer |
| CN110954686B (zh) * | 2014-07-31 | 2021-09-10 | 伊鲁米那股份有限公司 | 混合纳米孔传感器 |
| KR20170042794A (ko) | 2014-09-01 | 2017-04-19 | 브이아이비 브이지더블유 | 돌연변이체 csgg 포어 |
| US10266885B2 (en) | 2014-10-07 | 2019-04-23 | Oxford Nanopore Technologies Ltd. | Mutant pores |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| CN115851894B (zh) | 2014-10-16 | 2025-06-27 | 牛津楠路珀尔科技股份有限公司 | 聚合物的分析 |
| GB201418512D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Electrical device with detachable components |
| GB201418469D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
| JP6824881B2 (ja) | 2014-10-17 | 2021-02-03 | オックスフォード ナノポール テクノロジーズ リミテッド | ナノ細孔ｒｎａを特徴付けるための方法 |
| US11639522B2 (en) | 2015-01-30 | 2023-05-02 | President And Fellows Of Harvard College | Microscope-free imaging |
| CN112816679B (zh) | 2015-02-05 | 2024-05-28 | 哈佛大学校长及研究员协会 | 一种用于感测分子穿过纳米孔的移位的方法 |
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| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| US11169138B2 (en) | 2015-04-14 | 2021-11-09 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| GB201508003D0 (en) | 2015-05-11 | 2015-06-24 | Oxford Nanopore Tech Ltd | Apparatus and methods for measuring an electrical current |
| GB201508669D0 (en) | 2015-05-20 | 2015-07-01 | Oxford Nanopore Tech Ltd | Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown |
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| US10976300B2 (en) | 2015-12-08 | 2021-04-13 | Katholieke Universiteit Leuven | Modified nanopores, compositions comprising the same, and uses thereof |
| EP3916107B1 (en) * | 2016-02-17 | 2023-04-05 | President and Fellows of Harvard College | Molecular programming tools |
| CN116200476A (zh) | 2016-03-02 | 2023-06-02 | 牛津纳米孔科技公开有限公司 | 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔 |
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| CN109196116B (zh) | 2016-05-25 | 2022-09-27 | 牛津纳米孔科技公开有限公司 | 一种表征靶多核苷酸的方法 |
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| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| US11999948B2 (en) * | 2017-05-04 | 2024-06-04 | The Regents Of The University Of California | Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same |
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| CN110914290A (zh) | 2017-06-30 | 2020-03-24 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
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| GB2569977A (en) | 2018-01-05 | 2019-07-10 | Oxford Nanopore Tech Ltd | Method |
| WO2019147945A1 (en) | 2018-01-26 | 2019-08-01 | President And Fellows Of Harvard College | Proximity detection methods and compositions |
| GB201801768D0 (en) | 2018-02-02 | 2018-03-21 | Oxford Nanopore Tech Ltd | Synthesis method |
| EP3768852B1 (en) | 2018-03-22 | 2023-08-30 | President and Fellows of Harvard College | Methods and compositions for molecular authentication |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
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| GB201808556D0 (en) | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| US20220162692A1 (en) | 2018-07-30 | 2022-05-26 | Oxford University Innovation Limited | Assemblies |
| JP2020031557A (ja) * | 2018-08-28 | 2020-03-05 | 株式会社日立ハイテクノロジーズ | 生体分子分析装置 |
| CN112805393A (zh) * | 2018-09-28 | 2021-05-14 | 北京齐碳科技有限公司 | 一种解旋酶及其应用 |
| WO2020084705A1 (ja) * | 2018-10-24 | 2020-04-30 | 株式会社日立ハイテク | 生体ポリマ分析デバイス及びそれを用いた分析装置、並びに分析方法 |
| EP3650558A1 (en) * | 2018-11-07 | 2020-05-13 | Siemens Healthcare GmbH | Liquid sample workflow for nanopore sequencing |
| GB201821155D0 (en) | 2018-12-21 | 2019-02-06 | Oxford Nanopore Tech Ltd | Method |
| CN121090823A (zh) | 2019-03-12 | 2025-12-09 | 牛津纳米孔科技公开有限公司 | 纳米孔感测装置、组件和操作方法 |
| GB201907246D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| GB201907244D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| US11926819B2 (en) | 2019-05-28 | 2024-03-12 | The Regents Of The University Of California | Methods of adding polymers to ribonucleic acids |
| JP7612659B2 (ja) * | 2019-07-31 | 2025-01-14 | エーエックスバイオ インコーポレイテッド | 標的分子を評価するためのシステム及び方法 |
| WO2021056599A1 (zh) | 2019-09-29 | 2021-04-01 | 北京齐碳科技有限公司 | 一种Mnep单体变体及其应用 |
| GB201917060D0 (en) | 2019-11-22 | 2020-01-08 | Oxford Nanopore Tech Ltd | Method |
| CN114761799A (zh) | 2019-12-02 | 2022-07-15 | 牛津纳米孔科技公开有限公司 | 使用纳米孔表征靶多肽的方法 |
| GB201917742D0 (en) | 2019-12-04 | 2020-01-15 | Oxford Nanopore Tech Ltd | Method |
| WO2021124468A1 (ja) * | 2019-12-18 | 2021-06-24 | 株式会社日立ハイテク | 分子複合体、および生体ポリマ分析方法 |
| NL2024579B1 (en) * | 2019-12-24 | 2021-09-06 | Univ Delft Tech | Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes |
| GB202004944D0 (en) | 2020-04-03 | 2020-05-20 | King S College London | Method |
| EP4165210A1 (en) | 2020-06-10 | 2023-04-19 | F. Hoffmann-La Roche AG | Faradaic systems and methods for self-limiting protein pore insertion in a membrane |
| WO2021255475A1 (en) | 2020-06-18 | 2021-12-23 | Oxford Nanopore Technologies Limited | A method of selectively characterising a polynucleotide using a detector |
| US20240076729A9 (en) | 2020-06-18 | 2024-03-07 | Oxford Nanopore Technologies Plc | Method |
| GB202009349D0 (en) | 2020-06-18 | 2020-08-05 | Oxford Nanopore Tech Ltd | Method |
| CN113930406B (zh) | 2021-12-17 | 2022-04-08 | 北京齐碳科技有限公司 | 一种Pif1-like解旋酶及其应用 |
| KR20220124741A (ko) | 2020-06-19 | 2022-09-14 | 치탄 테크놀로지 리미티드 베이징 | Pif1 유사 헬리카제 및 이의 용도 |
| KR20230038566A (ko) | 2020-07-17 | 2023-03-20 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 나노포어 감지 디바이스 |
| CN116368095A (zh) | 2020-07-22 | 2023-06-30 | 牛津纳米孔科技公开有限公司 | 固态纳米孔形成 |
| GB202015993D0 (en) | 2020-10-08 | 2020-11-25 | Oxford Nanopore Tech Ltd | Method |
| WO2022126304A1 (zh) * | 2020-12-14 | 2022-06-23 | 北京齐碳科技有限公司 | 一种经修饰的解旋酶及其应用 |
| CN115777019A (zh) | 2021-04-06 | 2023-03-10 | 成都齐碳科技有限公司 | 经修饰的Prp43解旋酶及其用途 |
| GB202107192D0 (en) | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
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| GB202107354D0 (en) | 2021-05-24 | 2021-07-07 | Oxford Nanopore Tech Ltd | Method |
| GB202112235D0 (en) | 2021-08-26 | 2021-10-13 | Oxford Nanopore Tech Ltd | Nanopore |
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| GB202118906D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202205617D0 (en) | 2022-04-14 | 2022-06-01 | Oxford Nanopore Tech Plc | Novel modified protein pores and enzymes |
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| WO2023222657A1 (en) | 2022-05-17 | 2023-11-23 | Oxford Nanopore Technologies Plc | Method and adaptors |
| WO2023225988A1 (zh) * | 2022-05-27 | 2023-11-30 | 深圳华大生命科学研究院 | 一种维持纳米孔测序速度的方法 |
| GB202211607D0 (en) | 2022-08-09 | 2022-09-21 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| WO2024033443A1 (en) | 2022-08-09 | 2024-02-15 | Oxford Nanopore Technologies Plc | Novel pore monomers and pores |
| GB202211602D0 (en) | 2022-08-09 | 2022-09-21 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| KR20250096716A (ko) | 2022-10-28 | 2025-06-27 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 포어 단량체 및 포어 |
| GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| EP4636094A1 (en) * | 2022-12-16 | 2025-10-22 | BGI Shenzhen | Nanopore sequencing method and kit |
| GB202304324D0 (en) | 2023-03-24 | 2023-05-10 | Oxford Nanopore Tech Plc | Method and kits |
| GB202305524D0 (en) | 2023-04-14 | 2023-05-31 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202307141D0 (en) | 2023-05-12 | 2023-06-28 | Oxford Nanopore Tech Plc | Modified helicases |
| GB202307486D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Plc | Method |
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