CN115777019A - 经修饰的Prp43解旋酶及其用途 - Google Patents
经修饰的Prp43解旋酶及其用途 Download PDFInfo
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- CN115777019A CN115777019A CN202180006254.2A CN202180006254A CN115777019A CN 115777019 A CN115777019 A CN 115777019A CN 202180006254 A CN202180006254 A CN 202180006254A CN 115777019 A CN115777019 A CN 115777019A
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- prp43
- helicase
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- modified
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Abstract
一种经修饰的Prp43解旋酶及其用途。由于突变的引入和/或辅助蛋白的引入而增强了Prp43解旋酶的ATP水解活性或解旋活性，能长时间保持与目标多核苷酸的结合，从而允许酶以测序要求的合适速率、持续稳定地控制多核苷酸的移动速度，可以用于纳米孔测序。
Description
PCT国内申请，说明书已公开。
Claims (46)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2021/085609 WO2022213253A1 (zh) | 2021-04-06 | 2021-04-06 | 经修饰的Prp43解旋酶及其用途 |
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| Publication Number | Publication Date |
|---|---|
| CN115777019A true CN115777019A (zh) | 2023-03-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202180006254.2A Pending CN115777019A (zh) | 2021-04-06 | 2021-04-06 | 经修饰的Prp43解旋酶及其用途 |
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| Country | Link |
|---|---|
| EP (1) | EP4299746A4 (zh) |
| CN (1) | CN115777019A (zh) |
| WO (1) | WO2022213253A1 (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116334030A (zh) * | 2023-05-06 | 2023-06-27 | 深圳市梅丽纳米孔科技有限公司 | 一种经修饰的CfM HL4解旋酶及其应用 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2024089270A2 (en) * | 2022-10-28 | 2024-05-02 | Oxford Nanopore Technologies Plc | Pore monomers and pores |
| WO2024138701A1 (zh) * | 2022-12-30 | 2024-07-04 | 深圳华大生命科学研究院 | 一种解旋酶突变体及其制备方法和在高通量测序中的应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1390468A4 (en) * | 2001-04-23 | 2004-09-22 | Elitra Pharmaceuticals Inc | IDENTIFICATION OF ESSENTIAL GENES OF ASPERGILLUS FUMIGATUS AND METHOD OF USE |
| KR20140090633A (ko) | 2011-10-21 | 2014-07-17 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법 |
| US9617591B2 (en) | 2011-12-29 | 2017-04-11 | Oxford Nanopore Technologies Ltd. | Method for characterising a polynucleotide by using a XPD helicase |
| WO2014013260A1 (en) * | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Modified helicases |
| JP6677640B2 (ja) | 2013-10-18 | 2020-04-08 | オックスフォード ナノポール テクノロジーズ リミテッド | 修飾酵素 |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| GB201609220D0 (en) * | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| CN108676785A (zh) * | 2018-03-07 | 2018-10-19 | 天津市湖滨盘古基因科学发展有限公司 | 一种atp依赖型rna解旋酶dhx3突变蛋白及应用 |
| CN109266537B (zh) * | 2018-09-14 | 2019-10-29 | 首度生物科技（苏州）有限公司 | 使用单分子多次通过纳米孔达到精密测序的基因测序仪 |
| CN112147185B (zh) * | 2019-06-29 | 2022-07-01 | 清华大学 | 一种控制多肽穿过纳米孔速度的方法及其应用 |
-
2021
- 2021-04-06 EP EP21935490.9A patent/EP4299746A4/en active Pending
- 2021-04-06 CN CN202180006254.2A patent/CN115777019A/zh active Pending
- 2021-04-06 WO PCT/CN2021/085609 patent/WO2022213253A1/zh active Application Filing
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116334030A (zh) * | 2023-05-06 | 2023-06-27 | 深圳市梅丽纳米孔科技有限公司 | 一种经修饰的CfM HL4解旋酶及其应用 |
| CN116334030B (zh) * | 2023-05-06 | 2024-01-16 | 深圳市梅丽纳米孔科技有限公司 | 一种经修饰的CfM HL4解旋酶及其应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2022213253A1 (zh) | 2022-10-13 |
| EP4299746A1 (en) | 2024-01-03 |
| EP4299746A4 (en) | 2024-06-19 |
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