JP2014526253A - ポリ（α１，３グルカン）の高力価産生 - Google Patents
ポリ（α１，３グルカン）の高力価産生 Download PDFInfo
- Publication number
- JP2014526253A JP2014526253A JP2014529959A JP2014529959A JP2014526253A JP 2014526253 A JP2014526253 A JP 2014526253A JP 2014529959 A JP2014529959 A JP 2014529959A JP 2014529959 A JP2014529959 A JP 2014529959A JP 2014526253 A JP2014526253 A JP 2014526253A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- glucan
- poly
- primer
- glucosyltransferase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 229920005640 poly alpha-1,3-glucan Polymers 0.000 title claims abstract description 52
- 238000004519 manufacturing process Methods 0.000 title claims description 16
- 102000004190 Enzymes Human genes 0.000 claims abstract description 51
- 108090000790 Enzymes Proteins 0.000 claims abstract description 51
- 238000000034 method Methods 0.000 claims abstract description 28
- 108010055629 Glucosyltransferases Proteins 0.000 claims abstract description 27
- 102000000340 Glucosyltransferases Human genes 0.000 claims abstract description 27
- 229930006000 Sucrose Natural products 0.000 claims abstract description 25
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 25
- 239000005720 sucrose Substances 0.000 claims abstract description 25
- 229930091371 Fructose Natural products 0.000 claims abstract description 22
- 239000005715 Fructose Substances 0.000 claims abstract description 22
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims abstract description 22
- 239000012528 membrane Substances 0.000 claims abstract description 19
- 241000194024 Streptococcus salivarius Species 0.000 claims abstract description 14
- 238000006911 enzymatic reaction Methods 0.000 claims description 39
- 238000000502 dialysis Methods 0.000 claims description 15
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 230000001419 dependent effect Effects 0.000 claims description 8
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- 241000194017 Streptococcus Species 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 3
- 101150035967 gtf3 gene Proteins 0.000 claims description 2
- 101150116229 gtfB gene Proteins 0.000 claims description 2
- 101150055890 gtfC gene Proteins 0.000 claims description 2
- 101100176836 Streptococcus downei gtfI gene Proteins 0.000 claims 1
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- 239000000243 solution Substances 0.000 description 37
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 14
- 239000000835 fiber Substances 0.000 description 13
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 4
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 239000004698 Polyethylene Substances 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000013019 agitation Methods 0.000 description 3
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- 229920002678 cellulose Polymers 0.000 description 3
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- 150000002016 disaccharides Chemical class 0.000 description 3
- 150000002402 hexoses Chemical group 0.000 description 3
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 3
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- YTPMCWYIRHLEGM-BQYQJAHWSA-N 1-[(e)-2-propylsulfonylethenyl]sulfonylpropane Chemical compound CCCS(=O)(=O)\C=C\S(=O)(=O)CCC YTPMCWYIRHLEGM-BQYQJAHWSA-N 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- AFYNADDZULBEJA-UHFFFAOYSA-N bicinchoninic acid Chemical compound C1=CC=CC2=NC(C=3C=C(C4=CC=CC=C4N=3)C(=O)O)=CC(C(O)=O)=C21 AFYNADDZULBEJA-UHFFFAOYSA-N 0.000 description 2
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- 239000011780 sodium chloride Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 229960000999 sodium citrate dihydrate Drugs 0.000 description 2
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- DXALOGXSFLZLLN-WTZPKTTFSA-N (3s,4s,5r)-1,3,4,6-tetrahydroxy-5-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyhexan-2-one Chemical compound OCC(=O)[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O DXALOGXSFLZLLN-WTZPKTTFSA-N 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- YASYEJJMZJALEJ-UHFFFAOYSA-N Citric acid monohydrate Chemical compound O.OC(=O)CC(O)(C(O)=O)CC(O)=O YASYEJJMZJALEJ-UHFFFAOYSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- JPFGFRMPGVDDGE-UHFFFAOYSA-N Leucrose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)(CO)OC1 JPFGFRMPGVDDGE-UHFFFAOYSA-N 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- 241000194019 Streptococcus mutans Species 0.000 description 1
- 108700005078 Synthetic Genes Proteins 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- PLKYGPRDCKGEJH-UHFFFAOYSA-N azane;2-hydroxypropane-1,2,3-tricarboxylic acid;iron Chemical compound N.[Fe].OC(=O)CC(O)(C(O)=O)CC(O)=O PLKYGPRDCKGEJH-UHFFFAOYSA-N 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229960002303 citric acid monohydrate Drugs 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
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- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
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- 229910052751 metal Inorganic materials 0.000 description 1
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- 150000002772 monosaccharides Chemical class 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01005—Dextransucrase (2.4.1.5)
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Peptides Or Proteins (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- External Artificial Organs (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161532714P | 2011-09-09 | 2011-09-09 | |
| US61/532,714 | 2011-09-09 | ||
| PCT/US2012/054521 WO2013036968A1 (en) | 2011-09-09 | 2012-09-10 | HIGH TITER PRODUCTION OF POLY (α 1, 3 GLUCAN) |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2014526253A true JP2014526253A (ja) | 2014-10-06 |
| JP2014526253A5 JP2014526253A5 (cg-RX-API-DMAC7.html) | 2015-10-15 |
Family
ID=46832639
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014529959A Abandoned JP2014526253A (ja) | 2011-09-09 | 2012-09-10 | ポリ（α１，３グルカン）の高力価産生 |
Country Status (15)
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016514757A (ja) * | 2013-04-10 | 2016-05-23 | レンツィング アクチェンゲゼルシャフト | 多糖類フィルムおよび当該多糖類フィルムの製造のための方法 |
| JP2018507293A (ja) * | 2015-02-06 | 2018-03-15 | レンツィング アクチェンゲゼルシャフト | 多糖類懸濁液、その調製方法、及びその使用 |
| JP2018507929A (ja) * | 2015-02-06 | 2018-03-22 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | ポリα−１，３−グルカン系ポリマーのコロイド分散液 |
| JP2019500480A (ja) * | 2015-10-26 | 2019-01-10 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | 紙用多糖コーティング |
| WO2024261860A1 (ja) * | 2023-06-20 | 2024-12-26 | 日本電信電話株式会社 | 土中培養用装置および微生物の土壌適合性を評価する方法 |
Families Citing this family (124)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9365955B2 (en) * | 2011-12-30 | 2016-06-14 | Ei Du Pont De Nemours And Company | Fiber composition comprising 1,3-glucan and a method of preparing same |
| AU2013323686B2 (en) | 2012-09-25 | 2016-09-29 | Nutrition & Biosciences USA 4, Inc. | Glucosyltransferase enzymes for production of glucan polymers |
| US9139718B2 (en) | 2012-12-20 | 2015-09-22 | E I Du Pont De Nemours And Company | Preparation of poly alpha-1,3-glucan ethers |
| SG11201504218XA (en) | 2012-12-27 | 2015-07-30 | Du Pont | Preparation of poly alpha-1,3-glucan esters and films therefrom |
| AT514136A1 (de) | 2013-04-05 | 2014-10-15 | Lenzing Akiengesellschaft | Polysaccharidfaser mit erhöhtem Fibrillationsvermögen und Verfahren zu ihrer Herstellung |
| AT514137A1 (de) * | 2013-04-05 | 2014-10-15 | Lenzing Akiengesellschaft | Polysaccharidfaser und Verfahren zu ihrer Herstellung |
| AT514476A1 (de) * | 2013-06-17 | 2015-01-15 | Lenzing Akiengesellschaft | Polysaccharidfaser und Verfahren zu ihrer Herstellung |
| AT514475B1 (de) * | 2013-06-17 | 2016-11-15 | Chemiefaser Lenzing Ag | Polysaccharidfaser und Verfahren zu ihrer Herstellung |
| AT514468A1 (de) | 2013-06-17 | 2015-01-15 | Lenzing Akiengesellschaft | Hochsaugfähige Polysaccharidfaser und ihre Verwendung |
| AT514474B1 (de) | 2013-06-18 | 2016-02-15 | Chemiefaser Lenzing Ag | Polysaccharidfaser und Verfahren zu ihrer Herstellung |
| US9169506B2 (en) | 2013-09-05 | 2015-10-27 | E I Du Pont De Nemours And Company | Process for producing alpha-1,3-glucan polymer with reduced molecular weight |
| EP4163305B1 (en) | 2013-12-16 | 2024-07-10 | Nutrition & Biosciences USA 4, Inc. | Use of poly alpha-1,3-glucan ethers as viscosity modifiers |
| CN106029700B (zh) | 2013-12-18 | 2019-04-12 | 纳幕尔杜邦公司 | 阳离子聚α-1，3-葡聚糖醚 |
| EP3083776A1 (en) | 2013-12-20 | 2016-10-26 | E. I. du Pont de Nemours and Company | Films of poly alpha-1,3-glucan esters and method for their preparation |
| WO2015103531A1 (en) * | 2014-01-06 | 2015-07-09 | E. I. Du Pont De Nemours And Company | Production of poly alpha-1,3-glucan films |
| KR20160110383A (ko) * | 2014-01-17 | 2016-09-21 | 이 아이 듀폰 디 네모아 앤드 캄파니 | 가교-결합된 폴리 알파-1,3-글루칸의 용액의 제조 및 그로부터 제조된 폴리 알파-1,3-글루칸 필름 |
| KR20160107186A (ko) | 2014-01-17 | 2016-09-13 | 이 아이 듀폰 디 네모아 앤드 캄파니 | 폴리 알파-1,3-글루칸 포르메이트의 겔화된 네트워크의 제조 및 그로부터의 필름 |
| US10106626B2 (en) | 2014-01-17 | 2018-10-23 | Ei Du Pont De Nemours And Company | Production of poly alpha-1,3-glucan formate films |
| WO2015123323A1 (en) | 2014-02-14 | 2015-08-20 | E. I. Du Pont De Nemours And Company | Poly-alpha-1,3-1,6-glucans for viscosity modification |
| US9988657B2 (en) | 2014-02-27 | 2018-06-05 | E I Du Pont De Nemours And Company | Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes |
| WO2015130881A1 (en) * | 2014-02-27 | 2015-09-03 | E. I. Du Pont De Nemours And Company | Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes |
| ES2734726T3 (es) | 2014-03-11 | 2019-12-11 | Du Pont | Poli alfa-1,2-glucano oxidado como reforzante de detergente |
| JP6708554B2 (ja) | 2014-03-25 | 2020-06-10 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | 代替のスクロース原料からのグルカンポリマーの生成 |
| WO2015195777A1 (en) | 2014-06-19 | 2015-12-23 | E. I. Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| US9714403B2 (en) | 2014-06-19 | 2017-07-25 | E I Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| US20170198109A1 (en) | 2014-06-26 | 2017-07-13 | E I Du Pont De Nemours And Company | Production of poly alpha-1,3-glucan formate films |
| WO2015200590A1 (en) | 2014-06-26 | 2015-12-30 | E.I. Du Pont De Nemours And Company | Poly alpha-1,3-glucan solution compositions |
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| JP2016514757A (ja) * | 2013-04-10 | 2016-05-23 | レンツィング アクチェンゲゼルシャフト | 多糖類フィルムおよび当該多糖類フィルムの製造のための方法 |
| JP2018507293A (ja) * | 2015-02-06 | 2018-03-15 | レンツィング アクチェンゲゼルシャフト | 多糖類懸濁液、その調製方法、及びその使用 |
| JP2018507929A (ja) * | 2015-02-06 | 2018-03-22 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | ポリα−１，３−グルカン系ポリマーのコロイド分散液 |
| JP2019500480A (ja) * | 2015-10-26 | 2019-01-10 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | 紙用多糖コーティング |
| JP7365117B2 (ja) | 2015-10-26 | 2023-10-19 | ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド | 紙用多糖コーティング |
| WO2024261860A1 (ja) * | 2023-06-20 | 2024-12-26 | 日本電信電話株式会社 | 土中培養用装置および微生物の土壌適合性を評価する方法 |
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| AU2012305709B2 (en) | 2015-09-24 |
| PH12014500532A1 (en) | 2021-08-09 |
| RU2014113919A (ru) | 2015-10-20 |
| BR112014005322A2 (pt) | 2017-05-30 |
| SG11201400440YA (en) | 2014-04-28 |
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| AU2012305709A1 (en) | 2014-03-13 |
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| IL231088A0 (en) | 2014-03-31 |
| CN103764834A (zh) | 2014-04-30 |
| KR20140063764A (ko) | 2014-05-27 |
| WO2013036968A1 (en) | 2013-03-14 |
| CL2014000572A1 (es) | 2014-10-17 |
| EP2753703A1 (en) | 2014-07-16 |
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