JP2014525247A5 - - Google Patents
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- JP2014525247A5 JP2014525247A5 JP2014527227A JP2014527227A JP2014525247A5 JP 2014525247 A5 JP2014525247 A5 JP 2014525247A5 JP 2014527227 A JP2014527227 A JP 2014527227A JP 2014527227 A JP2014527227 A JP 2014527227A JP 2014525247 A5 JP2014525247 A5 JP 2014525247A5
- Authority
- JP
- Japan
- Prior art keywords
- subunit
- subunit complex
- cell
- antibody
- host cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 210000004027 cell Anatomy 0.000 claims 123
- 108090000623 proteins and genes Proteins 0.000 claims 87
- 238000000034 method Methods 0.000 claims 32
- 241000235648 Pichia Species 0.000 claims 26
- 108010076504 Protein Sorting Signals Proteins 0.000 claims 23
- 210000005253 yeast cell Anatomy 0.000 claims 18
- 210000003783 haploid cell Anatomy 0.000 claims 12
- 239000002609 medium Substances 0.000 claims 12
- 210000001840 diploid cell Anatomy 0.000 claims 11
- 241000235058 Komagataella pastoris Species 0.000 claims 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 10
- 208000035199 Tetraploidy Diseases 0.000 claims 9
- 102000008100 Human Serum Albumin Human genes 0.000 claims 8
- 108091006905 Human Serum Albumin Proteins 0.000 claims 8
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 claims 7
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 claims 7
- 230000001939 inductive effect Effects 0.000 claims 7
- 102100036826 Aldehyde oxidase Human genes 0.000 claims 6
- 101000928314 Homo sapiens Aldehyde oxidase Proteins 0.000 claims 6
- 208000020584 Polyploidy Diseases 0.000 claims 6
- 238000012258 culturing Methods 0.000 claims 6
- 108010014251 Muramidase Proteins 0.000 claims 5
- 102000016943 Muramidase Human genes 0.000 claims 5
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims 5
- LXJXRIRHZLFYRP-UHFFFAOYSA-N glyceraldehyde 3-phosphate Chemical compound O=CC(O)COP(O)(O)=O LXJXRIRHZLFYRP-UHFFFAOYSA-N 0.000 claims 5
- 239000004325 lysozyme Substances 0.000 claims 5
- 229960000274 lysozyme Drugs 0.000 claims 5
- 235000010335 lysozyme Nutrition 0.000 claims 5
- 230000013011 mating Effects 0.000 claims 5
- 102000005606 Activins Human genes 0.000 claims 4
- 108010059616 Activins Proteins 0.000 claims 4
- 101100502336 Komagataella pastoris FLD1 gene Proteins 0.000 claims 4
- 108010047620 Phytohemagglutinins Proteins 0.000 claims 4
- 101100421128 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SEI1 gene Proteins 0.000 claims 4
- 239000000488 activin Substances 0.000 claims 4
- 230000001885 phytohemagglutinin Effects 0.000 claims 4
- 108020003175 receptors Proteins 0.000 claims 4
- 230000028327 secretion Effects 0.000 claims 4
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 claims 3
- 108090000932 Calcitonin Gene-Related Peptide Proteins 0.000 claims 3
- 102100025588 Calcitonin gene-related peptide 1 Human genes 0.000 claims 3
- 101150069554 HIS4 gene Proteins 0.000 claims 3
- 101001098868 Homo sapiens Proprotein convertase subtilisin/kexin type 9 Proteins 0.000 claims 3
- 108090001005 Interleukin-6 Proteins 0.000 claims 3
- 102000004889 Interleukin-6 Human genes 0.000 claims 3
- 102000015336 Nerve Growth Factor Human genes 0.000 claims 3
- 108010025020 Nerve Growth Factor Proteins 0.000 claims 3
- 241001452677 Ogataea methanolica Species 0.000 claims 3
- 241000283973 Oryctolagus cuniculus Species 0.000 claims 3
- 102100038955 Proprotein convertase subtilisin/kexin type 9 Human genes 0.000 claims 3
- 108060008682 Tumor Necrosis Factor Proteins 0.000 claims 3
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 claims 3
- 210000003527 eukaryotic cell Anatomy 0.000 claims 3
- 239000012634 fragment Substances 0.000 claims 3
- 238000004519 manufacturing process Methods 0.000 claims 3
- 229920001184 polypeptide Polymers 0.000 claims 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims 3
- 239000013587 production medium Substances 0.000 claims 3
- FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 claims 2
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 claims 2
- 102000013563 Acid Phosphatase Human genes 0.000 claims 2
- 108010051457 Acid Phosphatase Proteins 0.000 claims 2
- 102000007698 Alcohol dehydrogenase Human genes 0.000 claims 2
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims 2
- 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 claims 2
- 241000271566 Aves Species 0.000 claims 2
- 241000255789 Bombyx mori Species 0.000 claims 2
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 claims 2
- 102100039556 Galectin-4 Human genes 0.000 claims 2
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 claims 2
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims 2
- 241000238631 Hexapoda Species 0.000 claims 2
- 101000780443 Homo sapiens Alcohol dehydrogenase 1A Proteins 0.000 claims 2
- 101000608765 Homo sapiens Galectin-4 Proteins 0.000 claims 2
- 101001018100 Homo sapiens Lysozyme C Proteins 0.000 claims 2
- 101710122479 Isocitrate lyase 1 Proteins 0.000 claims 2
- 241000270322 Lepidosauria Species 0.000 claims 2
- 101150012394 PHO5 gene Proteins 0.000 claims 2
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims 2
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims 2
- 101150014136 SUC2 gene Proteins 0.000 claims 2
- 101100191147 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PHO3 gene Proteins 0.000 claims 2
- 108010051210 beta-Fructofuranosidase Proteins 0.000 claims 2
- 210000000349 chromosome Anatomy 0.000 claims 2
- 102000028557 immunoglobulin binding proteins Human genes 0.000 claims 2
- 108091009323 immunoglobulin binding proteins Proteins 0.000 claims 2
- 230000002401 inhibitory effect Effects 0.000 claims 2
- 239000001573 invertase Substances 0.000 claims 2
- 235000011073 invertase Nutrition 0.000 claims 2
- 230000003612 virological effect Effects 0.000 claims 2
- 241000283690 Bos taurus Species 0.000 claims 1
- 241000283707 Capra Species 0.000 claims 1
- 241000699666 Mus <mouse, genus> Species 0.000 claims 1
- 241001494479 Pecora Species 0.000 claims 1
- 241000700159 Rattus Species 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 210000004507 artificial chromosome Anatomy 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 210000000805 cytoplasm Anatomy 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 101150061302 och1 gene Proteins 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 101150015622 pyk gene Proteins 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161525307P | 2011-08-19 | 2011-08-19 | |
| US61/525,307 | 2011-08-19 | ||
| US13/466,795 | 2012-05-08 | ||
| US13/466,795 US11214610B2 (en) | 2010-12-01 | 2012-05-08 | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| PCT/US2012/051619 WO2013028635A1 (en) | 2011-08-19 | 2012-08-20 | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014525247A JP2014525247A (ja) | 2014-09-29 |
| JP2014525247A5 true JP2014525247A5 (OSRAM) | 2015-10-08 |
| JP6138788B2 JP6138788B2 (ja) | 2017-05-31 |
Family
ID=48802355
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014527227A Active JP6138788B2 (ja) | 2011-08-19 | 2012-08-20 | 形質転換した微生物、例えばピキア・パストリス中で抗体などのマルチサブユニットタンパク質を高濃度かつ高純度で生産するための多コピー法 |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP2744903B1 (OSRAM) |
| JP (1) | JP6138788B2 (OSRAM) |
| KR (1) | KR101978534B1 (OSRAM) |
| CN (1) | CN103890178A (OSRAM) |
| AR (1) | AR087612A1 (OSRAM) |
| AU (1) | AU2012299035B2 (OSRAM) |
| BR (1) | BR112014003841B1 (OSRAM) |
| CA (1) | CA2845579C (OSRAM) |
| DK (1) | DK2744903T3 (OSRAM) |
| IL (1) | IL230978B (OSRAM) |
| MX (2) | MX383145B (OSRAM) |
| SG (2) | SG10201609752YA (OSRAM) |
| TW (1) | TWI666318B (OSRAM) |
| WO (1) | WO2013028635A1 (OSRAM) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI670279B (zh) * | 2013-03-15 | 2019-09-01 | 美商艾爾德生物製藥股份有限公司 | 抗體純化及純度監測 |
| US10202630B2 (en) | 2013-03-15 | 2019-02-12 | Alderbio Holdings Llc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| JP6412552B2 (ja) * | 2013-03-15 | 2018-10-24 | アルダー・バイオファーマシューティカルズ・インコーポレーテッド | 酵母及び他の形質転換細胞中のポリペプチドの高収率発現のための温度シフト |
| JP6206873B2 (ja) * | 2013-09-30 | 2017-10-04 | 国立研究開発法人農業・食品産業技術総合研究機構 | ニワトリリゾチーム由来の分泌シグナルペプチドを用いたブタリゾチームの製造方法 |
| US10556945B2 (en) | 2014-03-21 | 2020-02-11 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| CN111973740A (zh) | 2014-03-21 | 2020-11-24 | 泰华制药国际有限公司 | 针对降钙素基因相关肽的拮抗剂抗体及其使用方法 |
| CA3036632A1 (en) | 2016-09-23 | 2018-03-29 | Teva Pharmaceuticals International Gmbh | Treating refractory migraine |
| EP4202035A1 (en) | 2021-12-22 | 2023-06-28 | Gelita AG | Improved expression of peptides |
| WO2025141786A1 (ja) * | 2023-12-27 | 2025-07-03 | 中外製薬株式会社 | 抗体の製造方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| WO1994021293A1 (en) | 1993-03-19 | 1994-09-29 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| DE69534530T2 (de) | 1994-08-12 | 2006-07-06 | Immunomedics, Inc. | Für b-zell-lymphom und leukämiezellen spezifische immunkonjugate und humane antikörper |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| AU1219000A (en) | 1998-10-22 | 2000-05-08 | Regents Of The University Of California, The | Functionally assembled antigen-specific intact recombinant antibody and a methodfor production thereof |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| US6406863B1 (en) * | 2000-06-23 | 2002-06-18 | Genetastix Corporation | High throughput generation and screening of fully human antibody repertoire in yeast |
| DK1678314T3 (da) * | 2003-10-22 | 2012-12-03 | Keck Graduate Inst | Fremgangsmåde til syntetisering af heteromultimere polypeptider i gær ved anvendelse af en haploid parringsstrategi |
| WO2008063302A2 (en) * | 2006-10-10 | 2008-05-29 | Keck Graduate Institute | Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy |
| CN101868477A (zh) | 2007-05-21 | 2010-10-20 | 奥尔德生物制药公司 | 新型兔抗体人源化方法和人源化的兔抗体 |
| TWI617576B (zh) * | 2007-05-21 | 2018-03-11 | 艾爾德生物控股有限責任公司 | 介白素-６（ｉｌ-６）的抗體及其等之用途 |
| US11214610B2 (en) | 2010-12-01 | 2022-01-04 | H. Lundbeck A/S | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
-
2012
- 2012-08-20 KR KR1020147007256A patent/KR101978534B1/ko active Active
- 2012-08-20 AU AU2012299035A patent/AU2012299035B2/en active Active
- 2012-08-20 BR BR112014003841-4A patent/BR112014003841B1/pt active IP Right Grant
- 2012-08-20 MX MX2018005554A patent/MX383145B/es unknown
- 2012-08-20 CA CA2845579A patent/CA2845579C/en active Active
- 2012-08-20 MX MX2014001951A patent/MX355890B/es active IP Right Grant
- 2012-08-20 WO PCT/US2012/051619 patent/WO2013028635A1/en not_active Ceased
- 2012-08-20 JP JP2014527227A patent/JP6138788B2/ja active Active
- 2012-08-20 EP EP12825119.6A patent/EP2744903B1/en active Active
- 2012-08-20 SG SG10201609752YA patent/SG10201609752YA/en unknown
- 2012-08-20 CN CN201280051222.5A patent/CN103890178A/zh active Pending
- 2012-08-20 DK DK12825119.6T patent/DK2744903T3/en active
- 2012-08-20 SG SG2014011878A patent/SG2014011878A/en unknown
- 2012-08-20 TW TW101130127A patent/TWI666318B/zh not_active IP Right Cessation
- 2012-08-21 AR ARP120103067A patent/AR087612A1/es unknown
-
2014
- 2014-02-13 IL IL230978A patent/IL230978B/en active IP Right Grant
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