TWI666318B - 在經轉形的微生物如巴斯德畢赤氏酵母中的多－複製策略用於多－次級單元的蛋白質如抗體之高效價且高純度生產技術 - Google Patents
在經轉形的微生物如巴斯德畢赤氏酵母中的多－複製策略用於多－次級單元的蛋白質如抗體之高效價且高純度生產技術 Download PDFInfo
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- C12P21/005—Glycopeptides, glycoproteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
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Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161525307P | 2011-08-19 | 2011-08-19 | |
| US61/525,307 | 2011-08-19 | ||
| US13/466,795 US11214610B2 (en) | 2010-12-01 | 2012-05-08 | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| US13/466,795 | 2012-05-08 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW201313899A TW201313899A (zh) | 2013-04-01 |
| TWI666318B true TWI666318B (zh) | 2019-07-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW101130127A TWI666318B (zh) | 2011-08-19 | 2012-08-20 | 在經轉形的微生物如巴斯德畢赤氏酵母中的多－複製策略用於多－次級單元的蛋白質如抗體之高效價且高純度生產技術 |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP2744903B1 (OSRAM) |
| JP (1) | JP6138788B2 (OSRAM) |
| KR (1) | KR101978534B1 (OSRAM) |
| CN (1) | CN103890178A (OSRAM) |
| AR (1) | AR087612A1 (OSRAM) |
| AU (1) | AU2012299035B2 (OSRAM) |
| BR (1) | BR112014003841B1 (OSRAM) |
| CA (1) | CA2845579C (OSRAM) |
| DK (1) | DK2744903T3 (OSRAM) |
| IL (1) | IL230978B (OSRAM) |
| MX (2) | MX383145B (OSRAM) |
| SG (2) | SG10201609752YA (OSRAM) |
| TW (1) | TWI666318B (OSRAM) |
| WO (1) | WO2013028635A1 (OSRAM) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201940691A (zh) | 2013-03-15 | 2019-10-16 | 美商艾爾德生物製藥股份有限公司 | 抗體純化及純度監測 |
| US10202630B2 (en) | 2013-03-15 | 2019-02-12 | Alderbio Holdings Llc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| SG11201506034TA (en) | 2013-03-15 | 2015-09-29 | Alder Biopharmaceuticals Inc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| JP6206873B2 (ja) * | 2013-09-30 | 2017-10-04 | 国立研究開発法人農業・食品産業技術総合研究機構 | ニワトリリゾチーム由来の分泌シグナルペプチドを用いたブタリゾチームの製造方法 |
| US10556945B2 (en) | 2014-03-21 | 2020-02-11 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| US9896502B2 (en) | 2014-03-21 | 2018-02-20 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| WO2018055574A1 (en) | 2016-09-23 | 2018-03-29 | Teva Pharmaceuticals International Gmbh | Treating refractory migraine |
| EP4202035A1 (en) | 2021-12-22 | 2023-06-28 | Gelita AG | Improved expression of peptides |
| WO2025141786A1 (ja) * | 2023-12-27 | 2025-07-03 | 中外製薬株式会社 | 抗体の製造方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060270045A1 (en) * | 2003-10-22 | 2006-11-30 | Keck Graduate Institute | Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy |
Family Cites Families (16)
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| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| AU6445894A (en) | 1993-03-19 | 1994-10-11 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| WO1996004925A1 (en) | 1994-08-12 | 1996-02-22 | Immunomedics, Inc. | Immunoconjugates and humanized antibodies specific for b-cell lymphoma and leukemia cells |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| AU1219000A (en) | 1998-10-22 | 2000-05-08 | Regents Of The University Of California, The | Functionally assembled antigen-specific intact recombinant antibody and a methodfor production thereof |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| US6406863B1 (en) * | 2000-06-23 | 2002-06-18 | Genetastix Corporation | High throughput generation and screening of fully human antibody repertoire in yeast |
| WO2008063302A2 (en) * | 2006-10-10 | 2008-05-29 | Keck Graduate Institute | Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy |
| JP5859202B2 (ja) | 2007-05-21 | 2016-02-10 | アルダーバイオ・ホールディングズ・エルエルシー | 新規のウサギ抗体ヒト化方法及びヒト化ウサギ抗体 |
| SI3187506T1 (sl) * | 2007-05-21 | 2019-08-30 | Alderbio Holdings Llc | Protitelesa proti IL-6 in njihova uporaba |
| US11214610B2 (en) | 2010-12-01 | 2022-01-04 | H. Lundbeck A/S | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
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2012
- 2012-08-20 JP JP2014527227A patent/JP6138788B2/ja active Active
- 2012-08-20 WO PCT/US2012/051619 patent/WO2013028635A1/en not_active Ceased
- 2012-08-20 EP EP12825119.6A patent/EP2744903B1/en active Active
- 2012-08-20 KR KR1020147007256A patent/KR101978534B1/ko active Active
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- 2012-08-20 MX MX2018005554A patent/MX383145B/es unknown
- 2012-08-20 SG SG10201609752YA patent/SG10201609752YA/en unknown
- 2012-08-20 CN CN201280051222.5A patent/CN103890178A/zh active Pending
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060270045A1 (en) * | 2003-10-22 | 2006-11-30 | Keck Graduate Institute | Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy |
Non-Patent Citations (2)
| Title |
|---|
| 2005年02月，On the optimal ratio of heavy to light chain genes for efficient recombinant antibody production by CHO cells，Biotechnol Prog. 2005 Jan-Feb;21(1):122-33. |
| 年02月，On the optimal ratio of heavy to light chain genes for efficient recombinant antibody production by CHO cells，Biotechnol Prog. 2005 Jan-Feb;21(1):122-33. * |
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| AR087612A1 (es) | 2014-04-03 |
| AU2012299035B2 (en) | 2017-11-02 |
| BR112014003841B1 (pt) | 2022-02-15 |
| EP2744903A4 (en) | 2014-12-31 |
| CN103890178A (zh) | 2014-06-25 |
| CA2845579A1 (en) | 2013-02-28 |
| MX355890B (es) | 2018-05-03 |
| JP6138788B2 (ja) | 2017-05-31 |
| MX2014001951A (es) | 2016-02-05 |
| TW201313899A (zh) | 2013-04-01 |
| KR20140057613A (ko) | 2014-05-13 |
| BR112014003841A2 (pt) | 2017-03-14 |
| IL230978B (en) | 2018-08-30 |
| WO2013028635A9 (en) | 2013-09-26 |
| EP2744903A1 (en) | 2014-06-25 |
| NZ721102A (en) | 2020-01-31 |
| IL230978A0 (en) | 2014-03-31 |
| AU2012299035A1 (en) | 2014-03-13 |
| KR101978534B1 (ko) | 2019-08-28 |
| CA2845579C (en) | 2020-03-24 |
| MX383145B (es) | 2025-03-13 |
| JP2014525247A (ja) | 2014-09-29 |
| NZ621199A (en) | 2016-07-29 |
| WO2013028635A1 (en) | 2013-02-28 |
| SG10201609752YA (en) | 2017-01-27 |
| EP2744903B1 (en) | 2018-10-10 |
| SG2014011878A (en) | 2014-04-28 |
| DK2744903T3 (en) | 2019-01-28 |
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