CA2845579C - Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris - Google Patents
Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris Download PDFInfo
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- CA2845579C CA2845579C CA2845579A CA2845579A CA2845579C CA 2845579 C CA2845579 C CA 2845579C CA 2845579 A CA2845579 A CA 2845579A CA 2845579 A CA2845579 A CA 2845579A CA 2845579 C CA2845579 C CA 2845579C
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
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Applications Claiming Priority (5)
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| US201161525307P | 2011-08-19 | 2011-08-19 | |
| US61/525,307 | 2011-08-19 | ||
| US13/466,795 US11214610B2 (en) | 2010-12-01 | 2012-05-08 | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| US13/466,795 | 2012-05-08 | ||
| PCT/US2012/051619 WO2013028635A1 (en) | 2011-08-19 | 2012-08-20 | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris |
Publications (2)
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| CA2845579A1 CA2845579A1 (en) | 2013-02-28 |
| CA2845579C true CA2845579C (en) | 2020-03-24 |
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| CA2845579A Active CA2845579C (en) | 2011-08-19 | 2012-08-20 | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris |
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| JP (1) | JP6138788B2 (OSRAM) |
| KR (1) | KR101978534B1 (OSRAM) |
| CN (1) | CN103890178A (OSRAM) |
| AR (1) | AR087612A1 (OSRAM) |
| AU (1) | AU2012299035B2 (OSRAM) |
| BR (1) | BR112014003841B1 (OSRAM) |
| CA (1) | CA2845579C (OSRAM) |
| DK (1) | DK2744903T3 (OSRAM) |
| IL (1) | IL230978B (OSRAM) |
| MX (2) | MX355890B (OSRAM) |
| SG (2) | SG2014011878A (OSRAM) |
| TW (1) | TWI666318B (OSRAM) |
| WO (1) | WO2013028635A1 (OSRAM) |
Families Citing this family (9)
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| US10202630B2 (en) | 2013-03-15 | 2019-02-12 | Alderbio Holdings Llc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| EP3754012A1 (en) | 2013-03-15 | 2020-12-23 | Alder Biopharmaceuticals, Inc. | Antibody purification and purity monitoring |
| JP6412552B2 (ja) | 2013-03-15 | 2018-10-24 | アルダー・バイオファーマシューティカルズ・インコーポレーテッド | 酵母及び他の形質転換細胞中のポリペプチドの高収率発現のための温度シフト |
| JP6206873B2 (ja) * | 2013-09-30 | 2017-10-04 | 国立研究開発法人農業・食品産業技術総合研究機構 | ニワトリリゾチーム由来の分泌シグナルペプチドを用いたブタリゾチームの製造方法 |
| US10556945B2 (en) | 2014-03-21 | 2020-02-11 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| HRP20240159T1 (hr) | 2014-03-21 | 2024-04-12 | Teva Pharmaceuticals International Gmbh | Antagonistička protutijela usmjerena protiv peptida povezanog s genom kalcitonina i postupci koji ih koriste |
| WO2018055574A1 (en) | 2016-09-23 | 2018-03-29 | Teva Pharmaceuticals International Gmbh | Treating refractory migraine |
| EP4202035A1 (en) | 2021-12-22 | 2023-06-28 | Gelita AG | Improved expression of peptides |
| WO2025141786A1 (ja) * | 2023-12-27 | 2025-07-03 | 中外製薬株式会社 | 抗体の製造方法 |
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| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| WO1994021293A1 (en) | 1993-03-19 | 1994-09-29 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| WO1996004925A1 (en) | 1994-08-12 | 1996-02-22 | Immunomedics, Inc. | Immunoconjugates and humanized antibodies specific for b-cell lymphoma and leukemia cells |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| AU1219000A (en) | 1998-10-22 | 2000-05-08 | Regents Of The University Of California, The | Functionally assembled antigen-specific intact recombinant antibody and a methodfor production thereof |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| US6406863B1 (en) * | 2000-06-23 | 2002-06-18 | Genetastix Corporation | High throughput generation and screening of fully human antibody repertoire in yeast |
| ES2630224T3 (es) | 2003-10-22 | 2017-08-18 | Keck Graduate Institute | Métodos para sintetizar polipéptidos heteromultiméricos en levadura con el uso de una estrategia de apareamiento haploide |
| WO2008063302A2 (en) | 2006-10-10 | 2008-05-29 | Keck Graduate Institute | Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy |
| KR101829776B1 (ko) | 2007-05-21 | 2018-02-19 | 앨더바이오 홀딩스 엘엘씨 | Ｉｌ-６에 대한 항체 및 이의 용도 |
| TWI501976B (zh) | 2007-05-21 | 2015-10-01 | Alder Biopharmaceuticals Inc | 抗ＴＮＦ－α之抗體及其用途 |
| US11214610B2 (en) | 2010-12-01 | 2022-01-04 | H. Lundbeck A/S | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
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2012
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- 2012-08-20 JP JP2014527227A patent/JP6138788B2/ja active Active
- 2012-08-20 WO PCT/US2012/051619 patent/WO2013028635A1/en not_active Ceased
- 2012-08-20 CA CA2845579A patent/CA2845579C/en active Active
- 2012-08-20 BR BR112014003841-4A patent/BR112014003841B1/pt active IP Right Grant
- 2012-08-20 SG SG2014011878A patent/SG2014011878A/en unknown
- 2012-08-20 KR KR1020147007256A patent/KR101978534B1/ko active Active
- 2012-08-20 SG SG10201609752YA patent/SG10201609752YA/en unknown
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- 2012-08-20 EP EP12825119.6A patent/EP2744903B1/en active Active
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- 2014-02-13 IL IL230978A patent/IL230978B/en active IP Right Grant
Also Published As
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| TWI666318B (zh) | 2019-07-21 |
| KR20140057613A (ko) | 2014-05-13 |
| IL230978B (en) | 2018-08-30 |
| TW201313899A (zh) | 2013-04-01 |
| KR101978534B1 (ko) | 2019-08-28 |
| BR112014003841B1 (pt) | 2022-02-15 |
| SG2014011878A (en) | 2014-04-28 |
| EP2744903B1 (en) | 2018-10-10 |
| AU2012299035B2 (en) | 2017-11-02 |
| IL230978A0 (en) | 2014-03-31 |
| WO2013028635A9 (en) | 2013-09-26 |
| NZ721102A (en) | 2020-01-31 |
| SG10201609752YA (en) | 2017-01-27 |
| AU2012299035A1 (en) | 2014-03-13 |
| JP2014525247A (ja) | 2014-09-29 |
| EP2744903A1 (en) | 2014-06-25 |
| AR087612A1 (es) | 2014-04-03 |
| MX383145B (es) | 2025-03-13 |
| EP2744903A4 (en) | 2014-12-31 |
| DK2744903T3 (en) | 2019-01-28 |
| CA2845579A1 (en) | 2013-02-28 |
| MX355890B (es) | 2018-05-03 |
| JP6138788B2 (ja) | 2017-05-31 |
| MX2014001951A (es) | 2016-02-05 |
| CN103890178A (zh) | 2014-06-25 |
| NZ621199A (en) | 2016-07-29 |
| WO2013028635A1 (en) | 2013-02-28 |
| BR112014003841A2 (pt) | 2017-03-14 |
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