JP2014507952A5 - - Google Patents
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- JP2014507952A5 JP2014507952A5 JP2013557186A JP2013557186A JP2014507952A5 JP 2014507952 A5 JP2014507952 A5 JP 2014507952A5 JP 2013557186 A JP2013557186 A JP 2013557186A JP 2013557186 A JP2013557186 A JP 2013557186A JP 2014507952 A5 JP2014507952 A5 JP 2014507952A5
- Authority
- JP
- Japan
- Prior art keywords
- selectable marker
- nucleic acid
- recombinant nucleic
- seq
- promoter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 150000007523 nucleic acids Chemical class 0.000 claims description 14
- 108020004707 nucleic acids Proteins 0.000 claims description 10
- 102000039446 nucleic acids Human genes 0.000 claims description 10
- 241000233675 Thraustochytrium Species 0.000 claims description 7
- 108090000704 Tubulin Proteins 0.000 claims description 6
- 102000004243 Tubulin Human genes 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 101000912235 Rebecca salina Acyl-lipid (7-3)-desaturase Proteins 0.000 claims description 3
- 101000877236 Siganus canaliculatus Acyl-CoA Delta-4 desaturase Proteins 0.000 claims description 3
- 238000000034 method Methods 0.000 claims 27
- 239000003550 marker Substances 0.000 claims 8
- 108010084455 Zeocin Proteins 0.000 claims 5
- 101150038738 ble gene Proteins 0.000 claims 5
- 239000002773 nucleotide Substances 0.000 claims 5
- 125000003729 nucleotide group Chemical group 0.000 claims 5
- CWCMIVBLVUHDHK-ZSNHEYEWSA-N phleomycin D1 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC[C@@H](N=1)C=1SC=C(N=1)C(=O)NCCCCNC(N)=N)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C CWCMIVBLVUHDHK-ZSNHEYEWSA-N 0.000 claims 5
- 230000003115 biocidal effect Effects 0.000 claims 4
- 239000001963 growth medium Substances 0.000 claims 4
- 239000002245 particle Substances 0.000 claims 3
- 150000003839 salts Chemical class 0.000 claims 3
- 239000003242 anti bacterial agent Substances 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 1
- 230000014509 gene expression Effects 0.000 claims 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 1
- 239000010931 gold Substances 0.000 claims 1
- 229910052737 gold Inorganic materials 0.000 claims 1
- 230000035755 proliferation Effects 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 241001467333 Thraustochytriaceae Species 0.000 description 2
- 239000003744 tubulin modulator Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161449848P | 2011-03-07 | 2011-03-07 | |
| US61/449,848 | 2011-03-07 | ||
| PCT/IB2012/000528 WO2012120375A1 (en) | 2011-03-07 | 2012-03-07 | Engineering thraustochytrid microorganisms |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014507952A JP2014507952A (ja) | 2014-04-03 |
| JP2014507952A5 true JP2014507952A5 (https=) | 2015-08-06 |
| JP6168700B2 JP6168700B2 (ja) | 2017-07-26 |
Family
ID=46797543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013557186A Active JP6168700B2 (ja) | 2011-03-07 | 2012-03-07 | スラウストキトリド微生物の操作 |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US9133463B2 (https=) |
| EP (1) | EP2683824B1 (https=) |
| JP (1) | JP6168700B2 (https=) |
| CN (2) | CN107630017A (https=) |
| AU (1) | AU2012226528B2 (https=) |
| CA (1) | CA2829296C (https=) |
| DK (1) | DK2683824T3 (https=) |
| ES (1) | ES2678997T3 (https=) |
| MX (1) | MX338479B (https=) |
| NO (1) | NO2773848T3 (https=) |
| PT (1) | PT2683824T (https=) |
| WO (1) | WO2012120375A1 (https=) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2397655C (en) | 2000-01-19 | 2012-06-05 | Craig M. Ruecker | Solventless extraction process |
| EP2576801B1 (en) | 2010-06-01 | 2019-10-02 | DSM IP Assets B.V. | Extraction of lipid from cells and products therefrom |
| PT2683824T (pt) * | 2011-03-07 | 2018-06-07 | Dsm Nutritional Products Ag | Modificação de microorganismos thraustochytrid |
| EP2970926B1 (en) | 2013-03-13 | 2018-01-31 | DSM Nutritional Products AG | Engineering microorganisms |
| CN105939710B (zh) | 2013-12-20 | 2020-05-08 | 帝斯曼知识产权资产管理有限公司 | 用于从微生物细胞获得微生物油的方法 |
| CN105829539B (zh) | 2013-12-20 | 2023-11-10 | 帝斯曼营养品股份公司 | 从微生物中回收油的方法 |
| PL3083546T3 (pl) | 2013-12-20 | 2022-05-16 | Dsm Ip Assets B.V. | Sposoby wytwarzania oleju mikrobiologicznego z komórek mikrobiologicznych |
| FI3626806T3 (fi) | 2013-12-20 | 2024-08-22 | Mara Renewables Corp | Menetelmiä öljyn talteenottamiseksi mikro-organismeista |
| AU2014369040B2 (en) | 2013-12-20 | 2019-12-05 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| AR098895A1 (es) | 2013-12-20 | 2016-06-22 | Dsm Ip Assets Bv | Proceso para obtener aceite microbiano a partir de células microbianas |
| NZ721413A (en) | 2013-12-20 | 2022-05-27 | Dsm Ip Assets Bv | Processes for obtaining microbial oil from microbial cells |
| KR102236782B1 (ko) | 2014-05-22 | 2021-04-05 | 마라 리뉴어블즈 코퍼레이션 | 미생물에서 오일 생산 방법 |
| WO2016059541A1 (en) * | 2014-10-16 | 2016-04-21 | MARA Renewables Corporation | Repeated fed-batch culture methods |
| JP6806297B2 (ja) | 2014-10-16 | 2021-01-06 | マラ リニューアブルズ コーポレーション | 半連続培養法 |
| US9890402B2 (en) * | 2015-01-24 | 2018-02-13 | Indian Oil Corporation Limited | Thraustochytrid based process for treating waste effluents |
| CA2977271A1 (en) | 2015-03-02 | 2016-09-09 | Synthetic Genomics, Inc. | Regulatory elements from labyrinthulomycetes microorganisms |
| AU2016228545A1 (en) * | 2015-03-12 | 2017-09-21 | Synthetic Genomics. Inc. | Microorganisms for fatty acid production using elongase and desaturase enzymes |
| AR104042A1 (es) | 2015-03-26 | 2017-06-21 | Mara Renewables Corp | Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto |
| EP3318640A4 (en) * | 2015-07-03 | 2019-03-06 | Kuysyu University, National University Corporation | LABYRINTHULIDE MICROORGANISM FOR THE MANUFACTURE OF MICROBIAL OIL, MICROBIAL OIL, PROCESS FOR PREPARING THE SAID MICROORGANISM AND FOR PRODUCING THE SAID MICROBIAL OIL, AND USE OF THE SAID MICROORGANISM AND THE SAID MICROBIAL OIL |
| EP3322796A4 (en) | 2015-07-13 | 2019-03-27 | Mara Renewables Corporation | IMPROVEMENT OF MICROGENONAL FLUID CHANGES OF XYLOSE |
| FR3038914B1 (fr) * | 2015-07-17 | 2020-03-13 | Fermentalg | Biomasse de thraustochytrides, procede de culture et utilisations |
| CN106916796B (zh) * | 2015-12-28 | 2021-06-29 | 丰益(上海)生物技术研发中心有限公司 | 裂殖壶菌delta-12脂肪酸脱饱和酶相关序列及其应用 |
| EP3795586A3 (en) * | 2016-03-16 | 2021-06-23 | Conagen Inc. | Production of proteins in labyrinthulomycetes |
| US10851395B2 (en) | 2016-06-10 | 2020-12-01 | MARA Renewables Corporation | Method of making lipids with improved cold flow properties |
| US10676709B2 (en) | 2016-07-20 | 2020-06-09 | MARA Renewables Corporation | Flowable microbial oil |
| US10059906B2 (en) | 2016-07-20 | 2018-08-28 | MARA Renewables Corporation | Solventless winterization of microbial oil |
| US10633454B2 (en) | 2016-11-01 | 2020-04-28 | Conagen Inc. | Expression of modified glycoproteins and glycopeptides |
| EP3559206B1 (en) | 2016-12-22 | 2025-12-03 | Mara Renewables Corporation | Methods for producing biomass rich in dha, palmitic acid and protein using a eukaryotic microorganism |
| AU2019371162B2 (en) | 2018-11-02 | 2025-08-28 | MARA Renewables Corporation | Algal oil with improved nutritional value |
| CN109517834B (zh) * | 2018-11-27 | 2022-06-07 | 昆明藻能生物科技有限公司 | 一种通过遗传改造提高裂殖壶藻atcc20888油脂和dha含量的方法 |
| US20250129395A1 (en) | 2022-01-25 | 2025-04-24 | Dsm Ip Assets B.V. | Media refinement and nutrient feeding approaches to increase polyunsaturated fatty acid production |
| US20260002223A1 (en) * | 2022-06-27 | 2026-01-01 | Wisconsin Alumni Research Foundation | Universal method for parasite and eukaryotic endosymbiont identification |
| CN117721111B (zh) * | 2023-12-19 | 2024-06-07 | 海南省海洋与渔业科学院 | 红树植物白骨壤内源启动子amgt1p5及其应用 |
| CN119307499B (zh) * | 2024-12-18 | 2025-02-28 | 中国科学院天津工业生物技术研究所 | 内源tRNA启动子及破囊壶菌科微生物基因编辑方法 |
| CN120555215A (zh) * | 2025-07-29 | 2025-08-29 | 杭州合碳创物科技有限公司 | 一种高含量dha、低含量dpa的裂殖壶菌及其应用 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6451567B1 (en) | 1988-09-07 | 2002-09-17 | Omegatech, Inc. | Fermentation process for producing long chain omega-3 fatty acids with euryhaline microorganisms |
| US5340742A (en) | 1988-09-07 | 1994-08-23 | Omegatech Inc. | Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids |
| WO1997039106A1 (en) * | 1996-04-12 | 1997-10-23 | Martek Biosciences Corporation | Methods and tools for transformation of eukaryotic algae |
| US5968809A (en) | 1997-04-11 | 1999-10-19 | Abbot Laboratories | Methods and compositions for synthesis of long chain poly-unsaturated fatty acids |
| US8003772B2 (en) * | 1999-01-14 | 2011-08-23 | Martek Biosciences Corporation | Chimeric PUFA polyketide synthase systems and uses thereof |
| US7211418B2 (en) | 1999-01-14 | 2007-05-01 | Martek Biosciences Corporation | PUFA polyketide synthase systems and uses thereof |
| KR20070073986A (ko) | 2000-01-28 | 2007-07-10 | 마텍 바이오싸이언스스 코포레이션 | 발효기 내에서 진핵 미생물의 고밀도 배양에 의한고도불포화 지방산을 함유하는 지질의 증진된 생산 방법 |
| TWI377253B (en) | 2001-04-16 | 2012-11-21 | Martek Biosciences Corp | Product and process for transformation of thraustochytriales microorganisms |
| US7211656B2 (en) | 2002-01-30 | 2007-05-01 | Abbott Laboratories | Desaturase genes, enzymes encoded thereby, and uses thereof |
| JP4280158B2 (ja) | 2002-12-27 | 2009-06-17 | 富士フイルム株式会社 | ドコサヘキサエン酸生産能を有する微生物及びその利用 |
| US7214491B2 (en) | 2003-05-07 | 2007-05-08 | E. I. Du Pont De Nemours And Company | Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts |
| DE10352837A1 (de) * | 2003-11-10 | 2005-07-07 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Prozess zur Kultivierung von Mikroorganismen der Gattung Thraustochytriales |
| DE102004063326A1 (de) | 2004-12-23 | 2006-07-06 | Basf Plant Science Gmbh | Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Organismen |
| JP4796786B2 (ja) * | 2005-04-28 | 2011-10-19 | 富士フイルム株式会社 | ラビリンチュラ類を形質転換可能なベクター |
| JP4796787B2 (ja) | 2005-04-28 | 2011-10-19 | 富士フイルム株式会社 | ラビリンチュラ類への遺伝子導入法 |
| AU2006325040B2 (en) * | 2005-06-07 | 2012-04-19 | Dsm Nutritional Products Ag | Eukaryotic microorganisms for producing lipids and antioxidants |
| CN101473038B (zh) * | 2006-03-15 | 2014-05-28 | Dsmip资产公司 | 含有多不饱和脂肪酸的植物种子油 |
| CA2659603C (en) | 2006-08-01 | 2017-03-07 | Ocean Nutrition Canada Limited | Oil producing microbes and methods of modification thereof |
| WO2009010825A2 (en) * | 2007-07-13 | 2009-01-22 | Ocean Nutrition Canada Ltd. | D4 desaturases and d5 elongases |
| US8940884B2 (en) * | 2009-03-19 | 2015-01-27 | Dsm Ip Assets B.V. | Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof |
| WO2011037207A1 (ja) * | 2009-09-24 | 2011-03-31 | 国立大学法人九州大学 | ストラメノパイルの形質転換方法 |
| KR101068520B1 (ko) * | 2009-10-07 | 2011-09-28 | 대상 주식회사 | 고함량의 도코사헥사엔산을 함유하는 오메가-3 불포화 지방산을 생산하는 균주 및 이를 이용한 오메가-3 불포화 지방산의 제조방법 |
| IN2012DN06277A (https=) * | 2009-12-28 | 2015-09-25 | Dsm Ip Assets Bv | |
| US8765404B2 (en) | 2010-03-02 | 2014-07-01 | Massachusetts Institute Of Technology | Microbial engineering for the production of fatty acids and fatty acid derivatives |
| PT2683824T (pt) * | 2011-03-07 | 2018-06-07 | Dsm Nutritional Products Ag | Modificação de microorganismos thraustochytrid |
| EP2970926B1 (en) * | 2013-03-13 | 2018-01-31 | DSM Nutritional Products AG | Engineering microorganisms |
-
2012
- 2012-03-07 PT PT127550515T patent/PT2683824T/pt unknown
- 2012-03-07 CN CN201710887601.2A patent/CN107630017A/zh active Pending
- 2012-03-07 CA CA2829296A patent/CA2829296C/en not_active Expired - Fee Related
- 2012-03-07 EP EP12755051.5A patent/EP2683824B1/en not_active Not-in-force
- 2012-03-07 US US13/414,353 patent/US9133463B2/en active Active
- 2012-03-07 DK DK12755051.5T patent/DK2683824T3/en active
- 2012-03-07 CN CN201280018055.4A patent/CN103649313B/zh not_active Expired - Fee Related
- 2012-03-07 MX MX2013010167A patent/MX338479B/es active IP Right Grant
- 2012-03-07 AU AU2012226528A patent/AU2012226528B2/en not_active Ceased
- 2012-03-07 JP JP2013557186A patent/JP6168700B2/ja active Active
- 2012-03-07 ES ES12755051.5T patent/ES2678997T3/es active Active
- 2012-03-07 WO PCT/IB2012/000528 patent/WO2012120375A1/en not_active Ceased
- 2012-10-26 NO NO12845260A patent/NO2773848T3/no unknown
-
2015
- 2015-08-04 US US14/817,705 patent/US9758787B2/en active Active
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