CN103649313B - 工程化破囊壶菌属微生物 - Google Patents

工程化破囊壶菌属微生物 Download PDF

Info

Publication number
CN103649313B
CN103649313B CN201280018055.4A CN201280018055A CN103649313B CN 103649313 B CN103649313 B CN 103649313B CN 201280018055 A CN201280018055 A CN 201280018055A CN 103649313 B CN103649313 B CN 103649313B
Authority
CN
China
Prior art keywords
thraustochytrium
seq
cell
cells
onc
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201280018055.4A
Other languages
English (en)
Chinese (zh)
Other versions
CN103649313A (zh
Inventor
S.张
R.阿门塔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM Nutritional Products AG
Original Assignee
DSM Nutritional Products AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM Nutritional Products AG filed Critical DSM Nutritional Products AG
Priority to CN201710887601.2A priority Critical patent/CN107630017A/zh
Publication of CN103649313A publication Critical patent/CN103649313A/zh
Application granted granted Critical
Publication of CN103649313B publication Critical patent/CN103649313B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201280018055.4A 2011-03-07 2012-03-07 工程化破囊壶菌属微生物 Expired - Fee Related CN103649313B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710887601.2A CN107630017A (zh) 2011-03-07 2012-03-07 工程化破囊壶菌属微生物

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161449848P 2011-03-07 2011-03-07
US61/449848 2011-03-07
US61/449,848 2011-03-07
PCT/IB2012/000528 WO2012120375A1 (en) 2011-03-07 2012-03-07 Engineering thraustochytrid microorganisms

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201710887601.2A Division CN107630017A (zh) 2011-03-07 2012-03-07 工程化破囊壶菌属微生物

Publications (2)

Publication Number Publication Date
CN103649313A CN103649313A (zh) 2014-03-19
CN103649313B true CN103649313B (zh) 2017-10-24

Family

ID=46797543

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201280018055.4A Expired - Fee Related CN103649313B (zh) 2011-03-07 2012-03-07 工程化破囊壶菌属微生物
CN201710887601.2A Pending CN107630017A (zh) 2011-03-07 2012-03-07 工程化破囊壶菌属微生物

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201710887601.2A Pending CN107630017A (zh) 2011-03-07 2012-03-07 工程化破囊壶菌属微生物

Country Status (12)

Country Link
US (2) US9133463B2 (https=)
EP (1) EP2683824B1 (https=)
JP (1) JP6168700B2 (https=)
CN (2) CN103649313B (https=)
AU (1) AU2012226528B2 (https=)
CA (1) CA2829296C (https=)
DK (1) DK2683824T3 (https=)
ES (1) ES2678997T3 (https=)
MX (1) MX338479B (https=)
NO (1) NO2773848T3 (https=)
PT (1) PT2683824T (https=)
WO (1) WO2012120375A1 (https=)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2336307C2 (ru) 2000-01-19 2008-10-20 Мартек Биосайнсис Корпорейшн Способ получения липидов (варианты) и липиды, полученные этим способом
CN103124791B (zh) 2010-06-01 2016-08-24 帝斯曼知识产权资产管理有限公司 从细胞中提取脂质以及由此获得的产品
WO2012120375A1 (en) * 2011-03-07 2012-09-13 Ocean Nutrition Canada Limited Engineering thraustochytrid microorganisms
DK2970926T3 (en) * 2013-03-13 2018-04-16 Dsm Nutritional Products Ag GENMANIPULATION OF MICROorganisms
NZ721409A (en) 2013-12-20 2022-10-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
KR102416853B1 (ko) 2013-12-20 2022-07-05 마라 리뉴어블즈 코퍼레이션 미생물로부터 오일을 회수하는 방법
WO2015095693A2 (en) 2013-12-20 2015-06-25 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
JP6919827B2 (ja) 2013-12-20 2021-08-18 ディーエスエム ニュートリショナル プロダクツ アーゲーＤＳＭ Ｎｕｔｒｉｔｉｏｎａｌ Ｐｒｏｄｕｃｔｓ ＡＧ 微生物から油を回収する方法
PL3082793T3 (pl) 2013-12-20 2020-10-05 Dsm Ip Assets B.V. Sposoby otrzymywania oleju drobnoustrojowego z komórek drobnoustrojowych
SG11201605043TA (en) 2013-12-20 2016-07-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
AU2014369042B2 (en) 2013-12-20 2020-04-30 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
KR102236782B1 (ko) * 2014-05-22 2021-04-05 마라 리뉴어블즈 코퍼레이션 미생물에서 오일 생산 방법
WO2016059540A1 (en) 2014-10-16 2016-04-21 MARA Renewables Corporation Semi-continuous culture methods
EP3207165B1 (en) 2014-10-16 2020-02-05 Mara Renewables Corporation Repeated fed-batch culture methods
EP3098318A1 (en) * 2015-01-24 2016-11-30 Indian Oil Corporation Limited Thraustochytrid based process for treating waste effluents
EP3265567B1 (en) 2015-03-02 2020-07-29 Conagen Inc. Regulatory elements from labyrinthulomycetes microorganisms
AU2016228545A1 (en) * 2015-03-12 2017-09-21 Synthetic Genomics. Inc. Microorganisms for fatty acid production using elongase and desaturase enzymes
AR104042A1 (es) 2015-03-26 2017-06-21 Mara Renewables Corp Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto
WO2017006918A1 (ja) * 2015-07-03 2017-01-12 国立大学法人九州大学 微生物油産生ラビリンチュラ類、微生物油、ならびにそれらの作成方法およびそれらの使用
CA2991707C (en) * 2015-07-13 2023-08-01 MARA Renewables Corporation Enhancing microbial metabolism of c5 organic carbon
FR3038914B1 (fr) * 2015-07-17 2020-03-13 Fermentalg Biomasse de thraustochytrides, procede de culture et utilisations
CN106916796B (zh) * 2015-12-28 2021-06-29 丰益(上海)生物技术研发中心有限公司 裂殖壶菌delta-12脂肪酸脱饱和酶相关序列及其应用
AU2017235543B2 (en) * 2016-03-16 2020-05-14 Conagen, Inc. Production of proteins in labyrinthulomycetes
US10851395B2 (en) 2016-06-10 2020-12-01 MARA Renewables Corporation Method of making lipids with improved cold flow properties
ES2982227T3 (es) 2016-07-20 2024-10-15 Mara Renewables Corp Método de fraccionamiento en dos pasos para la winterización de aceite
BR112018076943B1 (pt) 2016-07-20 2023-02-07 MARA Renewables Corporation Método para obter óleo escoável
US10633454B2 (en) 2016-11-01 2020-04-28 Conagen Inc. Expression of modified glycoproteins and glycopeptides
EP3559206B1 (en) 2016-12-22 2025-12-03 Mara Renewables Corporation Methods for producing biomass rich in dha, palmitic acid and protein using a eukaryotic microorganism
JP7495066B2 (ja) 2018-11-02 2024-06-04 マラ リニューアブルズ コーポレーション 栄養価が改善した藻類オイル
CN109517834B (zh) * 2018-11-27 2022-06-07 昆明藻能生物科技有限公司 一种通过遗传改造提高裂殖壶藻atcc20888油脂和dha含量的方法
WO2023144707A1 (en) 2022-01-25 2023-08-03 Dsm Ip Assets B.V. Media refinement and nutrient feeding approaches to increase polyunsaturated fatty acid production
US20260002223A1 (en) * 2022-06-27 2026-01-01 Wisconsin Alumni Research Foundation Universal method for parasite and eukaryotic endosymbiont identification
CN117721111B (zh) * 2023-12-19 2024-06-07 海南省海洋与渔业科学院 红树植物白骨壤内源启动子amgt1p5及其应用
CN119307499B (zh) * 2024-12-18 2025-02-28 中国科学院天津工业生物技术研究所 内源tRNA启动子及破囊壶菌科微生物基因编辑方法
CN120555215A (zh) * 2025-07-29 2025-08-29 杭州合碳创物科技有限公司 一种高含量dha、低含量dpa的裂殖壶菌及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1556850A (zh) * 2001-04-16 2004-12-22 ��̩�������ѧ��˾ 转化破囊壶菌目微生物的产物和方法
WO2010108114A2 (en) * 2009-03-19 2010-09-23 Martek Biosciences Corporation Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US6451567B1 (en) 1988-09-07 2002-09-17 Omegatech, Inc. Fermentation process for producing long chain omega-3 fatty acids with euryhaline microorganisms
WO1997039106A1 (en) * 1996-04-12 1997-10-23 Martek Biosciences Corporation Methods and tools for transformation of eukaryotic algae
US5968809A (en) 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US8003772B2 (en) * 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7211418B2 (en) * 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
EP2960325B1 (en) 2000-01-28 2017-09-27 DSM IP Assets B.V. Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors
US7211656B2 (en) 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
JP4280158B2 (ja) 2002-12-27 2009-06-17 富士フイルム株式会社 ドコサヘキサエン酸生産能を有する微生物及びその利用
US7214491B2 (en) 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
DE10352837A1 (de) * 2003-11-10 2005-07-07 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Prozess zur Kultivierung von Mikroorganismen der Gattung Thraustochytriales
DE102004063326A1 (de) 2004-12-23 2006-07-06 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Organismen
JP4796786B2 (ja) 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類を形質転換可能なベクター
JP4796787B2 (ja) * 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類への遺伝子導入法
ES2626018T3 (es) * 2005-06-07 2017-07-21 Dsm Nutritional Products Ag Microorganismos eucariotas para la producción de lípidos y antioxidantes
CN101473038B (zh) * 2006-03-15 2014-05-28 Dsmip资产公司 含有多不饱和脂肪酸的植物种子油
CN101981201A (zh) 2006-08-01 2011-02-23 加拿大海洋营养食品有限公司 产油微生物及改良这些微生物的方法
US8765422B2 (en) * 2007-07-13 2014-07-01 Dsm Nutritional Products Ag D4 desaturases and D5 elongases
CA3121944A1 (en) * 2009-09-24 2011-03-31 Kyushu University, National University Corporation Method for transforming stramenopiles
KR101068520B1 (ko) * 2009-10-07 2011-09-28 대상 주식회사 고함량의 도코사헥사엔산을 함유하는 오메가-3 불포화 지방산을 생산하는 균주 및 이를 이용한 오메가-3 불포화 지방산의 제조방법
CA3028175C (en) 2009-12-28 2022-05-24 Sanofi Vaccine Technologies, S.A.S. Methods of producing heterologous viral neuraminidase proteins in microalgae
US8765404B2 (en) 2010-03-02 2014-07-01 Massachusetts Institute Of Technology Microbial engineering for the production of fatty acids and fatty acid derivatives
WO2012120375A1 (en) * 2011-03-07 2012-09-13 Ocean Nutrition Canada Limited Engineering thraustochytrid microorganisms
DK2970926T3 (en) * 2013-03-13 2018-04-16 Dsm Nutritional Products Ag GENMANIPULATION OF MICROorganisms

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1556850A (zh) * 2001-04-16 2004-12-22 ��̩�������ѧ��˾ 转化破囊壶菌目微生物的产物和方法
WO2010108114A2 (en) * 2009-03-19 2010-09-23 Martek Biosciences Corporation Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof

Also Published As

Publication number Publication date
US20120244584A1 (en) 2012-09-27
US9758787B2 (en) 2017-09-12
AU2012226528B2 (en) 2015-08-20
AU2012226528A1 (en) 2013-05-02
CA2829296C (en) 2019-10-29
US9133463B2 (en) 2015-09-15
CN103649313A (zh) 2014-03-19
CA2829296A1 (en) 2012-09-13
CN107630017A (zh) 2018-01-26
WO2012120375A1 (en) 2012-09-13
EP2683824A4 (en) 2015-05-06
NO2773848T3 (https=) 2018-03-31
JP6168700B2 (ja) 2017-07-26
DK2683824T3 (en) 2018-06-18
JP2014507952A (ja) 2014-04-03
ES2678997T3 (es) 2018-08-21
EP2683824B1 (en) 2018-04-25
MX2013010167A (es) 2014-04-14
EP2683824A1 (en) 2014-01-15
MX338479B (es) 2016-04-19
PT2683824T (pt) 2018-06-07
US20160010095A1 (en) 2016-01-14

Similar Documents

Publication Publication Date Title
CN103649313B (zh) 工程化破囊壶菌属微生物
Zhang et al. Alleviation of reactive oxygen species enhances PUFA accumulation in Schizochytrium sp. through regulating genes involved in lipid metabolism
AU2014229307B2 (en) Engineering microorganisms
US20220042052A1 (en) Labyrinthulid microorganism capable of producing microbial oil, microbial oil, methods for producing said microorganism and for producing said microbial oil, and uses of said microorganism and said microbial oil
JP2018519837A (ja) Ｃ５有機炭素の微生物による代謝の増強
JP6200807B2 (ja) 粘液細菌からの遺伝子集団の異種発現による脂肪酸の生産
JP7066593B2 (ja) 藻類改変体を含む飲食品、飼料、又は餌料
AU2017279745A1 (en) Engineering thraustochytrid microorganisms
US20160264985A1 (en) Microorganisms for fatty acid production using elongase and desaturase enzymes
AU2015252107A1 (en) Engineering thraustochytrid microorganisms
WO2022189976A1 (en) Genetic alterations in microalgae organisms, and methods and compositions
HK1193435A (en) Engineering thraustochytrid microorganisms
HK1193435B (en) Engineering thraustochytrid microorganisms
US20240182934A1 (en) Oil compositions with engineered lipid profiles and methods of producing same
CN121182660A (zh) 一株高产二十碳五烯酸的重组解脂耶氏酵母菌株及其构建方法和应用
HK1220485B (en) Engineering microorganisms
Okuda Biochemical analysis and molecular breeding of oleaginous

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C53 Correction of patent of invention or patent application
CB02 Change of applicant information

Address after: Swiss Kaiser August

Applicant after: DSM NUTRITIONAL PRODUCTS AG

Address before: American Maryland

Applicant before: DSM NUTRITIONAL PRODUCTS AG

GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20171024

Termination date: 20200307