MX338479B - Microorganismos thraustochytrid modificados. - Google Patents
Microorganismos thraustochytrid modificados.Info
- Publication number
- MX338479B MX338479B MX2013010167A MX2013010167A MX338479B MX 338479 B MX338479 B MX 338479B MX 2013010167 A MX2013010167 A MX 2013010167A MX 2013010167 A MX2013010167 A MX 2013010167A MX 338479 B MX338479 B MX 338479B
- Authority
- MX
- Mexico
- Prior art keywords
- microorganisms
- engineering
- thraustochytrium
- thraustochytrid
- methods
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6432—Eicosapentaenoic acids [EPA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6434—Docosahexenoic acids [DHA]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161449848P | 2011-03-07 | 2011-03-07 | |
| PCT/IB2012/000528 WO2012120375A1 (en) | 2011-03-07 | 2012-03-07 | Engineering thraustochytrid microorganisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2013010167A MX2013010167A (es) | 2014-04-14 |
| MX338479B true MX338479B (es) | 2016-04-19 |
Family
ID=46797543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013010167A MX338479B (es) | 2011-03-07 | 2012-03-07 | Microorganismos thraustochytrid modificados. |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US9133463B2 (https=) |
| EP (1) | EP2683824B1 (https=) |
| JP (1) | JP6168700B2 (https=) |
| CN (2) | CN103649313B (https=) |
| AU (1) | AU2012226528B2 (https=) |
| CA (1) | CA2829296C (https=) |
| DK (1) | DK2683824T3 (https=) |
| ES (1) | ES2678997T3 (https=) |
| MX (1) | MX338479B (https=) |
| NO (1) | NO2773848T3 (https=) |
| PT (1) | PT2683824T (https=) |
| WO (1) | WO2012120375A1 (https=) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2336307C2 (ru) | 2000-01-19 | 2008-10-20 | Мартек Биосайнсис Корпорейшн | Способ получения липидов (варианты) и липиды, полученные этим способом |
| CN103124791B (zh) | 2010-06-01 | 2016-08-24 | 帝斯曼知识产权资产管理有限公司 | 从细胞中提取脂质以及由此获得的产品 |
| WO2012120375A1 (en) * | 2011-03-07 | 2012-09-13 | Ocean Nutrition Canada Limited | Engineering thraustochytrid microorganisms |
| DK2970926T3 (en) * | 2013-03-13 | 2018-04-16 | Dsm Nutritional Products Ag | GENMANIPULATION OF MICROorganisms |
| NZ721409A (en) | 2013-12-20 | 2022-10-28 | Dsm Ip Assets Bv | Processes for obtaining microbial oil from microbial cells |
| KR102416853B1 (ko) | 2013-12-20 | 2022-07-05 | 마라 리뉴어블즈 코퍼레이션 | 미생물로부터 오일을 회수하는 방법 |
| WO2015095693A2 (en) | 2013-12-20 | 2015-06-25 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| JP6919827B2 (ja) | 2013-12-20 | 2021-08-18 | ディーエスエム ニュートリショナル プロダクツ アーゲーＤＳＭ Ｎｕｔｒｉｔｉｏｎａｌ Ｐｒｏｄｕｃｔｓ ＡＧ | 微生物から油を回収する方法 |
| PL3082793T3 (pl) | 2013-12-20 | 2020-10-05 | Dsm Ip Assets B.V. | Sposoby otrzymywania oleju drobnoustrojowego z komórek drobnoustrojowych |
| SG11201605043TA (en) | 2013-12-20 | 2016-07-28 | Dsm Ip Assets Bv | Processes for obtaining microbial oil from microbial cells |
| AU2014369042B2 (en) | 2013-12-20 | 2020-04-30 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| KR102236782B1 (ko) * | 2014-05-22 | 2021-04-05 | 마라 리뉴어블즈 코퍼레이션 | 미생물에서 오일 생산 방법 |
| WO2016059540A1 (en) | 2014-10-16 | 2016-04-21 | MARA Renewables Corporation | Semi-continuous culture methods |
| EP3207165B1 (en) | 2014-10-16 | 2020-02-05 | Mara Renewables Corporation | Repeated fed-batch culture methods |
| EP3098318A1 (en) * | 2015-01-24 | 2016-11-30 | Indian Oil Corporation Limited | Thraustochytrid based process for treating waste effluents |
| EP3265567B1 (en) | 2015-03-02 | 2020-07-29 | Conagen Inc. | Regulatory elements from labyrinthulomycetes microorganisms |
| AU2016228545A1 (en) * | 2015-03-12 | 2017-09-21 | Synthetic Genomics. Inc. | Microorganisms for fatty acid production using elongase and desaturase enzymes |
| AR104042A1 (es) | 2015-03-26 | 2017-06-21 | Mara Renewables Corp | Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto |
| WO2017006918A1 (ja) * | 2015-07-03 | 2017-01-12 | 国立大学法人九州大学 | 微生物油産生ラビリンチュラ類、微生物油、ならびにそれらの作成方法およびそれらの使用 |
| CA2991707C (en) * | 2015-07-13 | 2023-08-01 | MARA Renewables Corporation | Enhancing microbial metabolism of c5 organic carbon |
| FR3038914B1 (fr) * | 2015-07-17 | 2020-03-13 | Fermentalg | Biomasse de thraustochytrides, procede de culture et utilisations |
| CN106916796B (zh) * | 2015-12-28 | 2021-06-29 | 丰益(上海)生物技术研发中心有限公司 | 裂殖壶菌delta-12脂肪酸脱饱和酶相关序列及其应用 |
| AU2017235543B2 (en) * | 2016-03-16 | 2020-05-14 | Conagen, Inc. | Production of proteins in labyrinthulomycetes |
| US10851395B2 (en) | 2016-06-10 | 2020-12-01 | MARA Renewables Corporation | Method of making lipids with improved cold flow properties |
| ES2982227T3 (es) | 2016-07-20 | 2024-10-15 | Mara Renewables Corp | Método de fraccionamiento en dos pasos para la winterización de aceite |
| BR112018076943B1 (pt) | 2016-07-20 | 2023-02-07 | MARA Renewables Corporation | Método para obter óleo escoável |
| US10633454B2 (en) | 2016-11-01 | 2020-04-28 | Conagen Inc. | Expression of modified glycoproteins and glycopeptides |
| EP3559206B1 (en) | 2016-12-22 | 2025-12-03 | Mara Renewables Corporation | Methods for producing biomass rich in dha, palmitic acid and protein using a eukaryotic microorganism |
| JP7495066B2 (ja) | 2018-11-02 | 2024-06-04 | マラ リニューアブルズ コーポレーション | 栄養価が改善した藻類オイル |
| CN109517834B (zh) * | 2018-11-27 | 2022-06-07 | 昆明藻能生物科技有限公司 | 一种通过遗传改造提高裂殖壶藻atcc20888油脂和dha含量的方法 |
| WO2023144707A1 (en) | 2022-01-25 | 2023-08-03 | Dsm Ip Assets B.V. | Media refinement and nutrient feeding approaches to increase polyunsaturated fatty acid production |
| US20260002223A1 (en) * | 2022-06-27 | 2026-01-01 | Wisconsin Alumni Research Foundation | Universal method for parasite and eukaryotic endosymbiont identification |
| CN117721111B (zh) * | 2023-12-19 | 2024-06-07 | 海南省海洋与渔业科学院 | 红树植物白骨壤内源启动子amgt1p5及其应用 |
| CN119307499B (zh) * | 2024-12-18 | 2025-02-28 | 中国科学院天津工业生物技术研究所 | 内源tRNA启动子及破囊壶菌科微生物基因编辑方法 |
| CN120555215A (zh) * | 2025-07-29 | 2025-08-29 | 杭州合碳创物科技有限公司 | 一种高含量dha、低含量dpa的裂殖壶菌及其应用 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5340742A (en) | 1988-09-07 | 1994-08-23 | Omegatech Inc. | Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids |
| US6451567B1 (en) | 1988-09-07 | 2002-09-17 | Omegatech, Inc. | Fermentation process for producing long chain omega-3 fatty acids with euryhaline microorganisms |
| WO1997039106A1 (en) * | 1996-04-12 | 1997-10-23 | Martek Biosciences Corporation | Methods and tools for transformation of eukaryotic algae |
| US5968809A (en) | 1997-04-11 | 1999-10-19 | Abbot Laboratories | Methods and compositions for synthesis of long chain poly-unsaturated fatty acids |
| US8003772B2 (en) * | 1999-01-14 | 2011-08-23 | Martek Biosciences Corporation | Chimeric PUFA polyketide synthase systems and uses thereof |
| US7211418B2 (en) * | 1999-01-14 | 2007-05-01 | Martek Biosciences Corporation | PUFA polyketide synthase systems and uses thereof |
| EP2960325B1 (en) | 2000-01-28 | 2017-09-27 | DSM IP Assets B.V. | Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors |
| TWI324181B (en) * | 2001-04-16 | 2010-05-01 | Martek Biosciences Corp | Product and process for transformation of thraustochytriales microorganisms |
| US7211656B2 (en) | 2002-01-30 | 2007-05-01 | Abbott Laboratories | Desaturase genes, enzymes encoded thereby, and uses thereof |
| JP4280158B2 (ja) | 2002-12-27 | 2009-06-17 | 富士フイルム株式会社 | ドコサヘキサエン酸生産能を有する微生物及びその利用 |
| US7214491B2 (en) | 2003-05-07 | 2007-05-08 | E. I. Du Pont De Nemours And Company | Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts |
| DE10352837A1 (de) * | 2003-11-10 | 2005-07-07 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Prozess zur Kultivierung von Mikroorganismen der Gattung Thraustochytriales |
| DE102004063326A1 (de) | 2004-12-23 | 2006-07-06 | Basf Plant Science Gmbh | Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Organismen |
| JP4796786B2 (ja) | 2005-04-28 | 2011-10-19 | 富士フイルム株式会社 | ラビリンチュラ類を形質転換可能なベクター |
| JP4796787B2 (ja) * | 2005-04-28 | 2011-10-19 | 富士フイルム株式会社 | ラビリンチュラ類への遺伝子導入法 |
| ES2626018T3 (es) * | 2005-06-07 | 2017-07-21 | Dsm Nutritional Products Ag | Microorganismos eucariotas para la producción de lípidos y antioxidantes |
| CN101473038B (zh) * | 2006-03-15 | 2014-05-28 | Dsmip资产公司 | 含有多不饱和脂肪酸的植物种子油 |
| CN101981201A (zh) | 2006-08-01 | 2011-02-23 | 加拿大海洋营养食品有限公司 | 产油微生物及改良这些微生物的方法 |
| US8765422B2 (en) * | 2007-07-13 | 2014-07-01 | Dsm Nutritional Products Ag | D4 desaturases and D5 elongases |
| ES2717102T3 (es) * | 2009-03-19 | 2019-06-19 | Dsm Ip Assets Bv | Moléculas de ácido nucleico de ácido graso poliinsaturado sintasas, y polipéptidos, composiciones y métodos de preparación y usos de los mismos |
| CA3121944A1 (en) * | 2009-09-24 | 2011-03-31 | Kyushu University, National University Corporation | Method for transforming stramenopiles |
| KR101068520B1 (ko) * | 2009-10-07 | 2011-09-28 | 대상 주식회사 | 고함량의 도코사헥사엔산을 함유하는 오메가-3 불포화 지방산을 생산하는 균주 및 이를 이용한 오메가-3 불포화 지방산의 제조방법 |
| CA3028175C (en) | 2009-12-28 | 2022-05-24 | Sanofi Vaccine Technologies, S.A.S. | Methods of producing heterologous viral neuraminidase proteins in microalgae |
| US8765404B2 (en) | 2010-03-02 | 2014-07-01 | Massachusetts Institute Of Technology | Microbial engineering for the production of fatty acids and fatty acid derivatives |
| WO2012120375A1 (en) * | 2011-03-07 | 2012-09-13 | Ocean Nutrition Canada Limited | Engineering thraustochytrid microorganisms |
| DK2970926T3 (en) * | 2013-03-13 | 2018-04-16 | Dsm Nutritional Products Ag | GENMANIPULATION OF MICROorganisms |
-
2012
- 2012-03-07 WO PCT/IB2012/000528 patent/WO2012120375A1/en not_active Ceased
- 2012-03-07 ES ES12755051.5T patent/ES2678997T3/es active Active
- 2012-03-07 CN CN201280018055.4A patent/CN103649313B/zh not_active Expired - Fee Related
- 2012-03-07 JP JP2013557186A patent/JP6168700B2/ja active Active
- 2012-03-07 MX MX2013010167A patent/MX338479B/es active IP Right Grant
- 2012-03-07 CA CA2829296A patent/CA2829296C/en not_active Expired - Fee Related
- 2012-03-07 CN CN201710887601.2A patent/CN107630017A/zh active Pending
- 2012-03-07 EP EP12755051.5A patent/EP2683824B1/en not_active Not-in-force
- 2012-03-07 PT PT127550515T patent/PT2683824T/pt unknown
- 2012-03-07 AU AU2012226528A patent/AU2012226528B2/en not_active Ceased
- 2012-03-07 DK DK12755051.5T patent/DK2683824T3/en active
- 2012-03-07 US US13/414,353 patent/US9133463B2/en active Active
- 2012-10-26 NO NO12845260A patent/NO2773848T3/no unknown
-
2015
- 2015-08-04 US US14/817,705 patent/US9758787B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20120244584A1 (en) | 2012-09-27 |
| US9758787B2 (en) | 2017-09-12 |
| AU2012226528B2 (en) | 2015-08-20 |
| AU2012226528A1 (en) | 2013-05-02 |
| CA2829296C (en) | 2019-10-29 |
| US9133463B2 (en) | 2015-09-15 |
| CN103649313A (zh) | 2014-03-19 |
| CA2829296A1 (en) | 2012-09-13 |
| CN107630017A (zh) | 2018-01-26 |
| WO2012120375A1 (en) | 2012-09-13 |
| EP2683824A4 (en) | 2015-05-06 |
| NO2773848T3 (https=) | 2018-03-31 |
| JP6168700B2 (ja) | 2017-07-26 |
| DK2683824T3 (en) | 2018-06-18 |
| CN103649313B (zh) | 2017-10-24 |
| JP2014507952A (ja) | 2014-04-03 |
| ES2678997T3 (es) | 2018-08-21 |
| EP2683824B1 (en) | 2018-04-25 |
| MX2013010167A (es) | 2014-04-14 |
| EP2683824A1 (en) | 2014-01-15 |
| PT2683824T (pt) | 2018-06-07 |
| US20160010095A1 (en) | 2016-01-14 |
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