JP2014507950A5 - - Google Patents

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Publication number
JP2014507950A5
JP2014507950A5 JP2013556801A JP2013556801A JP2014507950A5 JP 2014507950 A5 JP2014507950 A5 JP 2014507950A5 JP 2013556801 A JP2013556801 A JP 2013556801A JP 2013556801 A JP2013556801 A JP 2013556801A JP 2014507950 A5 JP2014507950 A5 JP 2014507950A5
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JP
Japan
Prior art keywords
nucleic acid
strand
sequence
target
complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2013556801A
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English (en)
Japanese (ja)
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JP2014507950A (ja
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Publication date
Application filed filed Critical
Priority claimed from PCT/US2012/026938 external-priority patent/WO2012118802A1/en
Publication of JP2014507950A publication Critical patent/JP2014507950A/ja
Publication of JP2014507950A5 publication Critical patent/JP2014507950A5/ja
Pending legal-status Critical Current

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JP2013556801A 2011-02-28 2012-02-28 混合個体群における標的ｄｎａを配列決定するためのキットおよび方法 Pending JP2014507950A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161447490P 2011-02-28 2011-02-28
US61/447,490 2011-02-28
US201161532887P 2011-09-09 2011-09-09
US61/532,887 2011-09-09
PCT/US2012/026938 WO2012118802A1 (en) 2011-02-28 2012-02-28 Kit and method for sequencing a target dna in a mixed population

Publications (2)

Publication Number Publication Date
JP2014507950A JP2014507950A (ja) 2014-04-03
JP2014507950A5 true JP2014507950A5 (enExample) 2015-04-16

Family

ID=45815993

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013556801A Pending JP2014507950A (ja) 2011-02-28 2012-02-28 混合個体群における標的ｄｎａを配列決定するためのキットおよび方法

Country Status (8)

Country Link
US (1) US20120225421A1 (enExample)
EP (1) EP2681332A1 (enExample)
JP (1) JP2014507950A (enExample)
KR (1) KR20140010093A (enExample)
CN (1) CN103517993A (enExample)
AU (1) AU2012223438A1 (enExample)
CA (1) CA2828535A1 (enExample)
WO (1) WO2012118802A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2665500T3 (es) 2010-03-08 2018-04-26 Dana-Farber Cancer Institute, Inc. Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
WO2014089797A1 (zh) * 2012-12-13 2014-06-19 深圳华大基因科技服务有限公司 用于高通量测序的锁核酸修饰的dna片段
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
KR20160003444A (ko) * 2014-07-01 2016-01-11 주식회사 유진셀 핵산말단분해효소를 이용한 서열-특이적 핵산 검출 방법 및 장치와 이에 사용되는 키트
SG11201707154SA (en) * 2015-03-06 2017-09-28 Pillar Biosciences Inc Selective amplification of overlapping amplicons
JP2018518155A (ja) * 2015-04-15 2018-07-12 ザ ジェネラル ホスピタル コーポレイション Ｌｎａベース突然変異体エンリッチメント次世代シーケンシングアッセイ
CN107849606A (zh) * 2015-04-20 2018-03-27 尼欧基因组学实验室股份有限公司 提高下一代测序灵敏度的方法
WO2017070339A1 (en) * 2015-10-20 2017-04-27 Richardson Katherine Microfluidic device for enrichment of nucleic acid sequence alterations
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection
US11174511B2 (en) 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
CN109536594B (zh) * 2017-09-20 2022-11-01 深圳华大智造科技股份有限公司 一种用于Small RNA的测序方法、测序试剂和应用
EP3998338B1 (en) * 2019-07-11 2024-04-24 Tokyo University of Science Foundation Method for amplifying nucleic acid using solid-phase carrier
CN113567404A (zh) * 2021-06-11 2021-10-29 上海交通大学 一种分析肿瘤细胞耐药性的方法和试剂盒

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2293238A (en) * 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
FR2779154B1 (fr) * 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
US6045993A (en) * 1998-05-30 2000-04-04 Visible Genetics Inc. Method, reagent and kit for genotyping of human papillomavirus
DE10012540B4 (de) * 2000-03-15 2004-09-23 Vermicon Ag Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion
JP2004520812A (ja) * 2000-08-30 2004-07-15 ハプロゲン・エルエルシー 対立遺伝子を決定するための方法
GB0406863D0 (en) * 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
US8455190B2 (en) * 2007-08-01 2013-06-04 Dana-Farber Cancer Institute, Inc. Enrichment of a target sequence
US8071338B2 (en) * 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
ES2359058B1 (es) * 2009-07-02 2012-03-27 Consejo Superior De Investigaciones Cient�?Ficas (Csic) Quimera de adn polimerasa del fago ph1 29.
WO2011012534A1 (en) 2009-07-29 2011-02-03 Nerviano Medical Sciences S.R.L. Plk inhibitor salts
ES2665500T3 (es) 2010-03-08 2018-04-26 Dana-Farber Cancer Institute, Inc. Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia

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