JP2014507950A5 - - Google Patents
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- JP2014507950A5 JP2014507950A5 JP2013556801A JP2013556801A JP2014507950A5 JP 2014507950 A5 JP2014507950 A5 JP 2014507950A5 JP 2013556801 A JP2013556801 A JP 2013556801A JP 2013556801 A JP2013556801 A JP 2013556801A JP 2014507950 A5 JP2014507950 A5 JP 2014507950A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- strand
- sequence
- target
- complementary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000007523 nucleic acids Chemical class 0.000 claims 34
- 108020004707 nucleic acids Proteins 0.000 claims 32
- 102000039446 nucleic acids Human genes 0.000 claims 32
- 230000000903 blocking effect Effects 0.000 claims 27
- 238000012163 sequencing technique Methods 0.000 claims 22
- 230000000295 complement effect Effects 0.000 claims 16
- 238000000034 method Methods 0.000 claims 12
- 239000011541 reaction mixture Substances 0.000 claims 12
- 125000003729 nucleotide group Chemical group 0.000 claims 7
- 230000003321 amplification Effects 0.000 claims 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims 5
- 108091027305 Heteroduplex Proteins 0.000 claims 4
- 108091093037 Peptide nucleic acid Proteins 0.000 claims 4
- 229910019142 PO4 Inorganic materials 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 239000010452 phosphate Substances 0.000 claims 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 2
- 238000001712 DNA sequencing Methods 0.000 claims 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 2
- 108060002716 Exonuclease Proteins 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 230000015572 biosynthetic process Effects 0.000 claims 2
- 238000004925 denaturation Methods 0.000 claims 2
- 230000036425 denaturation Effects 0.000 claims 2
- 230000001419 dependent effect Effects 0.000 claims 2
- 102000013165 exonuclease Human genes 0.000 claims 2
- 238000002844 melting Methods 0.000 claims 2
- 230000008018 melting Effects 0.000 claims 2
- 230000002438 mitochondrial effect Effects 0.000 claims 2
- 230000035772 mutation Effects 0.000 claims 2
- 108020004705 Codon Proteins 0.000 claims 1
- 101000984753 Homo sapiens Serine/threonine-protein kinase B-raf Proteins 0.000 claims 1
- 101100193693 Kirsten murine sarcoma virus K-RAS gene Proteins 0.000 claims 1
- 208000035177 MELAS Diseases 0.000 claims 1
- 102100027103 Serine/threonine-protein kinase B-raf Human genes 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000012165 high-throughput sequencing Methods 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 238000012175 pyrosequencing Methods 0.000 claims 1
- 235000011178 triphosphate Nutrition 0.000 claims 1
- 239000001226 triphosphate Substances 0.000 claims 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161447490P | 2011-02-28 | 2011-02-28 | |
| US61/447,490 | 2011-02-28 | ||
| US201161532887P | 2011-09-09 | 2011-09-09 | |
| US61/532,887 | 2011-09-09 | ||
| PCT/US2012/026938 WO2012118802A1 (en) | 2011-02-28 | 2012-02-28 | Kit and method for sequencing a target dna in a mixed population |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2014507950A JP2014507950A (ja) | 2014-04-03 |
| JP2014507950A5 true JP2014507950A5 (enExample) | 2015-04-16 |
Family
ID=45815993
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013556801A Pending JP2014507950A (ja) | 2011-02-28 | 2012-02-28 | 混合個体群における標的ｄｎａを配列決定するためのキットおよび方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20120225421A1 (enExample) |
| EP (1) | EP2681332A1 (enExample) |
| JP (1) | JP2014507950A (enExample) |
| KR (1) | KR20140010093A (enExample) |
| CN (1) | CN103517993A (enExample) |
| AU (1) | AU2012223438A1 (enExample) |
| CA (1) | CA2828535A1 (enExample) |
| WO (1) | WO2012118802A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2665500T3 (es) | 2010-03-08 | 2018-04-26 | Dana-Farber Cancer Institute, Inc. | Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia |
| DK2691541T3 (en) | 2011-03-31 | 2018-01-22 | Dana Farber Cancer Inst Inc | PROCEDURE FOR ENRICHMENT OF SINGLE DRAWED MUTANTS SEQUENCES FROM A MIXTURE OF WILD TYPE AND MUTANTS |
| WO2014089797A1 (zh) * | 2012-12-13 | 2014-06-19 | 深圳华大基因科技服务有限公司 | 用于高通量测序的锁核酸修饰的dna片段 |
| WO2015013166A1 (en) | 2013-07-24 | 2015-01-29 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable enrichment of minor dna alleles by limiting denaturation time in pcr or simply enable enrichment of minor dna alleles by limiting denaturation time in pcr |
| KR20160003444A (ko) * | 2014-07-01 | 2016-01-11 | 주식회사 유진셀 | 핵산말단분해효소를 이용한 서열-특이적 핵산 검출 방법 및 장치와 이에 사용되는 키트 |
| US10011869B2 (en) * | 2015-03-06 | 2018-07-03 | Pillar Biosciences Inc. | Selective amplification of overlapping amplicons |
| KR20170136555A (ko) * | 2015-04-15 | 2017-12-11 | 더 제너럴 하스피탈 코포레이션 | Lna-기반 돌연변이체 농축 차세대 서열분석 검정 |
| CN107849606A (zh) * | 2015-04-20 | 2018-03-27 | 尼欧基因组学实验室股份有限公司 | 提高下一代测序灵敏度的方法 |
| WO2017070339A1 (en) * | 2015-10-20 | 2017-04-27 | Richardson Katherine | Microfluidic device for enrichment of nucleic acid sequence alterations |
| CA3046953A1 (en) | 2016-12-12 | 2018-06-21 | Dana Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection |
| WO2019023243A1 (en) | 2017-07-24 | 2019-01-31 | Dana-Farber Cancer Institute, Inc. | METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE |
| CN109536594B (zh) * | 2017-09-20 | 2022-11-01 | 深圳华大智造科技股份有限公司 | 一种用于Small RNA的测序方法、测序试剂和应用 |
| ES2981912T3 (es) * | 2019-07-11 | 2024-10-11 | Univ Tokyo Science Found | Método para amplificar un ácido nucleico usando un portador en fase sólida |
| CN113567404A (zh) * | 2021-06-11 | 2021-10-29 | 上海交通大学 | 一种分析肿瘤细胞耐药性的方法和试剂盒 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2293238A (en) * | 1994-09-13 | 1996-03-20 | Inceltec Ltd | Primers for replication and/or amplification reactions |
| US5849497A (en) * | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| FR2779154B1 (fr) * | 1998-05-27 | 2002-07-12 | Bio Merieux | Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre |
| US6045993A (en) * | 1998-05-30 | 2000-04-04 | Visible Genetics Inc. | Method, reagent and kit for genotyping of human papillomavirus |
| DE10012540B4 (de) * | 2000-03-15 | 2004-09-23 | Vermicon Ag | Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion |
| CN1293204C (zh) * | 2000-08-30 | 2007-01-03 | 戴诺生物技术有限公司 | 等位基因的测定方法 |
| GB0406863D0 (en) * | 2004-03-26 | 2004-04-28 | Qiagen As | Nucleic acid sequencing |
| DK2183379T3 (en) * | 2007-08-01 | 2015-07-27 | Dana Farber Cancer Inst Inc | ENRICHMENT OF A TARGET SEQUENCE |
| US8071338B2 (en) * | 2007-08-08 | 2011-12-06 | Roche Molecular Systems, Inc. | Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity |
| ES2359058B1 (es) * | 2009-07-02 | 2012-03-27 | Consejo Superior De Investigaciones Cient�?Ficas (Csic) | Quimera de adn polimerasa del fago ph1 29. |
| CN102470136B (zh) | 2009-07-29 | 2013-12-25 | 内尔维阿诺医学科学有限公司 | Plk 抑制剂的盐类 |
| ES2665500T3 (es) | 2010-03-08 | 2018-04-26 | Dana-Farber Cancer Institute, Inc. | Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia |
-
2012
- 2012-02-28 CN CN201280020801.3A patent/CN103517993A/zh active Pending
- 2012-02-28 JP JP2013556801A patent/JP2014507950A/ja active Pending
- 2012-02-28 EP EP12708466.3A patent/EP2681332A1/en not_active Withdrawn
- 2012-02-28 KR KR1020137025264A patent/KR20140010093A/ko not_active Withdrawn
- 2012-02-28 US US13/407,274 patent/US20120225421A1/en not_active Abandoned
- 2012-02-28 CA CA2828535A patent/CA2828535A1/en not_active Abandoned
- 2012-02-28 AU AU2012223438A patent/AU2012223438A1/en not_active Abandoned
- 2012-02-28 WO PCT/US2012/026938 patent/WO2012118802A1/en not_active Ceased
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