CN103517993A - 用于对混合群体中的靶dna进行测序的试剂盒和方法 - Google Patents

用于对混合群体中的靶dna进行测序的试剂盒和方法 Download PDF

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Publication number
CN103517993A
CN103517993A CN201280020801.3A CN201280020801A CN103517993A CN 103517993 A CN103517993 A CN 103517993A CN 201280020801 A CN201280020801 A CN 201280020801A CN 103517993 A CN103517993 A CN 103517993A
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nucleic acid
chain
sequencing
sequence
target
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Chinese (zh)
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K·理查森
B·小勒让德
Y·施
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Msf Gene Co Ltd
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Msf Gene Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/173—Nucleic acid detection characterized by the use of physical, structural and functional properties staining/intercalating agent, e.g. ethidium bromide

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201280020801.3A 2011-02-28 2012-02-28 用于对混合群体中的靶dna进行测序的试剂盒和方法 Pending CN103517993A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161447490P 2011-02-28 2011-02-28
US61/447,490 2011-02-28
US201161532887P 2011-09-09 2011-09-09
US61/532,887 2011-09-09
PCT/US2012/026938 WO2012118802A1 (en) 2011-02-28 2012-02-28 Kit and method for sequencing a target dna in a mixed population

Publications (1)

Publication Number Publication Date
CN103517993A true CN103517993A (zh) 2014-01-15

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CN201280020801.3A Pending CN103517993A (zh) 2011-02-28 2012-02-28 用于对混合群体中的靶dna进行测序的试剂盒和方法

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Country Link
US (1) US20120225421A1 (enExample)
EP (1) EP2681332A1 (enExample)
JP (1) JP2014507950A (enExample)
KR (1) KR20140010093A (enExample)
CN (1) CN103517993A (enExample)
AU (1) AU2012223438A1 (enExample)
CA (1) CA2828535A1 (enExample)
WO (1) WO2012118802A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107709557A (zh) * 2015-04-15 2018-02-16 通用医疗公司 基于lna的突变型富集下一代测序测定

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5795341B2 (ja) 2010-03-08 2015-10-14 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド 参照ブロック配列によるｆｕｌｌＣＯＬＤ−ＰＣＲ濃縮
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
WO2014089797A1 (zh) * 2012-12-13 2014-06-19 深圳华大基因科技服务有限公司 用于高通量测序的锁核酸修饰的dna片段
WO2015013166A1 (en) 2013-07-24 2015-01-29 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor dna alleles by limiting denaturation time in pcr or simply enable enrichment of minor dna alleles by limiting denaturation time in pcr
KR20160003444A (ko) * 2014-07-01 2016-01-11 주식회사 유진셀 핵산말단분해효소를 이용한 서열-특이적 핵산 검출 방법 및 장치와 이에 사용되는 키트
US10011869B2 (en) * 2015-03-06 2018-07-03 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
JP2018512878A (ja) * 2015-04-20 2018-05-24 ネオゲノミクス ラボラトリーズ， インコーポレイテッド 次世代シークエンシングの感度を高めるための方法
WO2017070339A1 (en) * 2015-10-20 2017-04-27 Richardson Katherine Microfluidic device for enrichment of nucleic acid sequence alterations
CA3046953A1 (en) 2016-12-12 2018-06-21 Dana Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
CN109536594B (zh) * 2017-09-20 2022-11-01 深圳华大智造科技股份有限公司 一种用于Small RNA的测序方法、测序试剂和应用
CN114096679B (zh) * 2019-07-11 2024-04-09 学校法人东京理科大学 使用固相载体的核酸扩增方法
CN113567404A (zh) * 2021-06-11 2021-10-29 上海交通大学 一种分析肿瘤细胞耐药性的方法和试剂盒

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2293238A (en) * 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
FR2779154B1 (fr) * 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
US6045993A (en) * 1998-05-30 2000-04-04 Visible Genetics Inc. Method, reagent and kit for genotyping of human papillomavirus
DE10012540B4 (de) * 2000-03-15 2004-09-23 Vermicon Ag Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion
CN1293204C (zh) * 2000-08-30 2007-01-03 戴诺生物技术有限公司 等位基因的测定方法
GB0406863D0 (en) * 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
KR101376359B1 (ko) * 2007-08-01 2014-03-27 다나-파버 캔서 인스티튜트 인크. 표적 서열 강화
US8071338B2 (en) * 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
ES2359058B1 (es) * 2009-07-02 2012-03-27 Consejo Superior De Investigaciones Cient�?Ficas (Csic) Quimera de adn polimerasa del fago ph1 29.
US8648078B2 (en) 2009-07-29 2014-02-11 Nerviano Medical Sciences S.R.L. PLK inhibitor salts
JP5795341B2 (ja) 2010-03-08 2015-10-14 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド 参照ブロック配列によるｆｕｌｌＣＯＬＤ−ＰＣＲ濃縮

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2293238A (en) * 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MILBURY COREN A ET AL: "Ice-COLD-PCR enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations", 《NUCLEIC ACIDS RESEARCH》, 11 October 2010 (2010-10-11), pages 1 - 10 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107709557A (zh) * 2015-04-15 2018-02-16 通用医疗公司 基于lna的突变型富集下一代测序测定
US10876151B2 (en) 2015-04-15 2020-12-29 The General Hospital Corporation LNA-based mutant enrichment next-generation sequencing assays

Also Published As

Publication number Publication date
WO2012118802A1 (en) 2012-09-07
AU2012223438A1 (en) 2013-09-26
KR20140010093A (ko) 2014-01-23
US20120225421A1 (en) 2012-09-06
WO2012118802A9 (en) 2013-07-18
JP2014507950A (ja) 2014-04-03
EP2681332A1 (en) 2014-01-08
CA2828535A1 (en) 2012-09-07

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