JP2014502272A - アフィニティークロマトグラフィーマトリックス - Google Patents

アフィニティークロマトグラフィーマトリックス Download PDF

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Publication number
JP2014502272A
JP2014502272A JP2013541958A JP2013541958A JP2014502272A JP 2014502272 A JP2014502272 A JP 2014502272A JP 2013541958 A JP2013541958 A JP 2013541958A JP 2013541958 A JP2013541958 A JP 2013541958A JP 2014502272 A JP2014502272 A JP 2014502272A
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JP
Japan
Prior art keywords
protein
ligand
domains
immunoglobulin
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2013541958A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014502272A5 (enExample
Inventor
ビヨルクマン，トーマス
ロドリゴ，グスタブ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Amersham Bioscience AB
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Bioscience AB, GE Healthcare Bio Sciences Corp filed Critical Amersham Bioscience AB
Publication of JP2014502272A publication Critical patent/JP2014502272A/ja
Publication of JP2014502272A5 publication Critical patent/JP2014502272A5/ja
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
JP2013541958A 2010-11-29 2011-11-28 アフィニティークロマトグラフィーマトリックス Pending JP2014502272A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41749410P 2010-11-29 2010-11-29
US61/417,494 2010-11-29
PCT/SE2011/051432 WO2012074463A1 (en) 2010-11-29 2011-11-28 Affinity chromatography matrix

Publications (2)

Publication Number Publication Date
JP2014502272A true JP2014502272A (ja) 2014-01-30
JP2014502272A5 JP2014502272A5 (enExample) 2015-01-15

Family

ID=46172160

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013541958A Pending JP2014502272A (ja) 2010-11-29 2011-11-28 アフィニティークロマトグラフィーマトリックス

Country Status (5)

Country Link
US (1) US8859726B2 (enExample)
EP (1) EP2646126A4 (enExample)
JP (1) JP2014502272A (enExample)
CN (1) CN103228328A (enExample)
WO (1) WO2012074463A1 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8592555B2 (en) 2008-08-11 2013-11-26 Emd Millipore Corporation Immunoglobulin-binding proteins with improved specificity
SG162687A1 (en) 2008-12-24 2010-07-29 Millipore Corp Caustic stable chromatography ligands
US9051375B2 (en) * 2010-12-21 2015-06-09 The University Of Western Ontario Alkali-resistant variants of protein A and their use in affinity chromatography
SG10201604559TA (en) 2011-06-08 2016-07-28 Emd Millipore Corp Chromatography matrices including novel staphylococcus aureus protein a based ligands
US9896486B2 (en) * 2013-07-10 2018-02-20 Ge Healthcare Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
ES2742505T3 (es) 2013-08-28 2020-02-14 Affibody Ab Polipéptidos de unión que tienen un andamio mutado
CN104513309A (zh) * 2013-09-26 2015-04-15 上海亨臻实业有限公司 一种蛋白功能结构域铰链肽及其用途
US11566082B2 (en) * 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
GB201506868D0 (en) 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method for protein purification
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
CN109311949B (zh) 2016-05-11 2022-09-16 思拓凡生物工艺研发有限公司 储存分离基质的方法
JP6987424B2 (ja) 2016-05-11 2022-01-05 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックスを洗浄および／または消毒する方法
WO2017194597A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Separation matrix
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
CN106117324A (zh) * 2016-07-29 2016-11-16 湖北爱晟生物科技有限公司 重组蛋白a及其编码基因和应用
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
GB201703116D0 (en) * 2017-02-27 2017-04-12 Ge Healthcare Bioprocess R&D Ab A seperation matrix and a method of seperating antibodies
GB2569585A (en) * 2017-12-20 2019-06-26 Ge Healthcare Bio Sciences Ab A method for preparation of a separation matrix
CN109721645A (zh) * 2017-12-29 2019-05-07 兆生生物科技南京有限公司 一种基因突变的蛋白a及其应用
EP3650465A1 (en) 2018-11-12 2020-05-13 Navigo Proteins Gmbh Novel populations of polypeptides having a triple-helical structure
US12344638B2 (en) 2019-04-02 2025-07-01 Bondwell Technologies Lp Functionalized UBX protein materials for enhanced purification of antibodies
GB202113626D0 (en) 2021-09-24 2021-11-10 Cytiva Bioprocess R & D Ab FC binding polypeptides
CN113999294B (zh) * 2021-12-30 2022-03-29 苏州纳微科技股份有限公司 免疫球蛋白结合蛋白及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050143566A1 (en) * 2002-03-25 2005-06-30 Sophia Hober Mutated immunoglobulin-binding protein
WO2005075507A1 (en) * 2004-02-09 2005-08-18 Affibody Ab Amyloid beta peptide binding polypeptide
JP2010081866A (ja) * 2008-09-30 2010-04-15 National Institute Of Advanced Industrial Science & Technology 弱酸性域での易解離性を向上したプロテインａ変異型タンパク質及び抗体捕捉剤

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Publication number Priority date Publication date Assignee Title
US5362859A (en) * 1992-07-27 1994-11-08 Sepracor, Inc. High-capacity affinity supports and methods for the preparation and use of same
SE0104353D0 (sv) * 2001-12-19 2001-12-19 Amersham Biosciences Ab Separation method
US7709209B2 (en) * 2002-03-25 2010-05-04 Ge Healthcare Bio-Sciences Ab Protein ligands
CN101522278B (zh) * 2006-09-29 2016-08-10 通用电气健康护理生物科学股份公司 用于抗体分离的包含来自金黄色葡萄球菌a蛋白的结构域c的层析配体
CN101224414A (zh) * 2007-10-18 2008-07-23 西北大学 以四唑为功能基的多用途分离介质及其制备方法
US20120149875A1 (en) 2009-01-12 2012-06-14 Ge Healthcare Bio-Sciences Ab Affinity chromatography matrix

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050143566A1 (en) * 2002-03-25 2005-06-30 Sophia Hober Mutated immunoglobulin-binding protein
US20060194950A1 (en) * 2002-03-25 2006-08-31 Sophia Hober Mutant protein
WO2005075507A1 (en) * 2004-02-09 2005-08-18 Affibody Ab Amyloid beta peptide binding polypeptide
JP2010081866A (ja) * 2008-09-30 2010-04-15 National Institute Of Advanced Industrial Science & Technology 弱酸性域での易解離性を向上したプロテインａ変異型タンパク質及び抗体捕捉剤

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JPN6015050981; 'Ｒｅｖｉｅｗ　：　Ｐｒｏｔｅｉｎ　Ａ　ｃｈｒｏｍａｔｏｇｒａｐｈｙ　ｆｏｒ　ａｎｔｉｂｏｄｙ　ｐｕｒｉｆｉｃａｔｉｏｎ' Ｊｏｕｒｎａｌ　ｏｆ　Ｃｈｒｏｍａｔｏｇｒａｐｈｙ　Ｂ Ｖｏｌ．８４８，ｐ．４０-４７, 2007 *

Also Published As

Publication number Publication date
US8859726B2 (en) 2014-10-14
EP2646126A4 (en) 2014-12-17
CN103228328A (zh) 2013-07-31
US20130245224A1 (en) 2013-09-19
WO2012074463A1 (en) 2012-06-07
EP2646126A1 (en) 2013-10-09

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