CN103228328A - 亲和色谱基质 - Google Patents

亲和色谱基质 Download PDF

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Publication number
CN103228328A
CN103228328A CN2011800565779A CN201180056577A CN103228328A CN 103228328 A CN103228328 A CN 103228328A CN 2011800565779 A CN2011800565779 A CN 2011800565779A CN 201180056577 A CN201180056577 A CN 201180056577A CN 103228328 A CN103228328 A CN 103228328A
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CN
China
Prior art keywords
domain
protein
amino acid
albumen
immunoglobulin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2011800565779A
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English (en)
Chinese (zh)
Inventor
T.毕约克曼
G.罗德里戈
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
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GE Healthcare Bio Sciences AB
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Filing date
Publication date
Application filed by GE Healthcare Bio Sciences AB filed Critical GE Healthcare Bio Sciences AB
Publication of CN103228328A publication Critical patent/CN103228328A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN2011800565779A 2010-11-29 2011-11-28 亲和色谱基质 Pending CN103228328A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41749410P 2010-11-29 2010-11-29
US61/417,494 2010-11-29
PCT/SE2011/051432 WO2012074463A1 (en) 2010-11-29 2011-11-28 Affinity chromatography matrix

Publications (1)

Publication Number Publication Date
CN103228328A true CN103228328A (zh) 2013-07-31

Family

ID=46172160

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011800565779A Pending CN103228328A (zh) 2010-11-29 2011-11-28 亲和色谱基质

Country Status (5)

Country Link
US (1) US8859726B2 (enExample)
EP (1) EP2646126A4 (enExample)
JP (1) JP2014502272A (enExample)
CN (1) CN103228328A (enExample)
WO (1) WO2012074463A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104513309A (zh) * 2013-09-26 2015-04-15 上海亨臻实业有限公司 一种蛋白功能结构域铰链肽及其用途
CN106117324A (zh) * 2016-07-29 2016-11-16 湖北爱晟生物科技有限公司 重组蛋白a及其编码基因和应用
CN109721645A (zh) * 2017-12-29 2019-05-07 兆生生物科技南京有限公司 一种基因突变的蛋白a及其应用
CN111511467A (zh) * 2017-12-20 2020-08-07 通用电气医疗集团生物工艺研发股份公司 制备分离基质的方法

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8592555B2 (en) 2008-08-11 2013-11-26 Emd Millipore Corporation Immunoglobulin-binding proteins with improved specificity
SG162687A1 (en) 2008-12-24 2010-07-29 Millipore Corp Caustic stable chromatography ligands
US9051375B2 (en) * 2010-12-21 2015-06-09 The University Of Western Ontario Alkali-resistant variants of protein A and their use in affinity chromatography
SG10201604554WA (en) 2011-06-08 2016-07-28 Emd Millipore Corp Chromatography matrices including novel staphylococcus aureus protein a based ligands
US9896486B2 (en) * 2013-07-10 2018-02-20 Ge Healthcare Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
KR102497083B1 (ko) 2013-08-28 2023-02-07 애피바디 에이비 돌연변이된 스캐폴드를 갖는 결합 폴리펩티드
US11566082B2 (en) * 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
GB201506868D0 (en) 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method for protein purification
ES2909833T3 (es) 2016-05-11 2022-05-10 Cytiva Bioprocess R & D Ab Método de limpieza y/o desinfección de una matriz de separación
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
US11708390B2 (en) 2016-05-11 2023-07-25 Cytiva Bioprocess R&D Ab Method of storing a separation matrix
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
EP3455243B1 (en) 2016-05-11 2021-03-24 Cytiva BioProcess R&D AB Separation matrix
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
GB201703116D0 (en) * 2017-02-27 2017-04-12 Ge Healthcare Bioprocess R&D Ab A seperation matrix and a method of seperating antibodies
EP3650465A1 (en) * 2018-11-12 2020-05-13 Navigo Proteins Gmbh Novel populations of polypeptides having a triple-helical structure
EP3947473A1 (en) * 2019-04-02 2022-02-09 Bondwell Technologies Inc. Functionalized ubx protein materials for enhanced purification of antibodies
GB202113626D0 (en) 2021-09-24 2021-11-10 Cytiva Bioprocess R & D Ab FC binding polypeptides
CN113999294B (zh) * 2021-12-30 2022-03-29 苏州纳微科技股份有限公司 免疫球蛋白结合蛋白及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050043522A1 (en) * 2001-12-19 2005-02-24 Jean-Luc Maloisel Separation method
WO2005075507A1 (en) * 2004-02-09 2005-08-18 Affibody Ab Amyloid beta peptide binding polypeptide
CN101224414A (zh) * 2007-10-18 2008-07-23 西北大学 以四唑为功能基的多用途分离介质及其制备方法
EP1972689A2 (en) * 2002-03-25 2008-09-24 GE Healthcare Bio-Sciences AB A mutated immunoglobulin-binding protein

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5362859A (en) * 1992-07-27 1994-11-08 Sepracor, Inc. High-capacity affinity supports and methods for the preparation and use of same
US7709209B2 (en) * 2002-03-25 2010-05-04 Ge Healthcare Bio-Sciences Ab Protein ligands
JP5345539B2 (ja) 2006-09-29 2013-11-20 ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー 抗体単離のためのスタフィロコッカスアウレウス由来のドメインｃを含むクロマトグラフィーリガンド
JP5229888B2 (ja) * 2008-09-30 2013-07-03 独立行政法人産業技術総合研究所 弱酸性域での易解離性を向上したプロテインａ変異型タンパク質及び抗体捕捉剤
JP2012515160A (ja) * 2009-01-12 2012-07-05 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ アフィニティークロマトグラフィーマトリックス

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050043522A1 (en) * 2001-12-19 2005-02-24 Jean-Luc Maloisel Separation method
EP1972689A2 (en) * 2002-03-25 2008-09-24 GE Healthcare Bio-Sciences AB A mutated immunoglobulin-binding protein
WO2005075507A1 (en) * 2004-02-09 2005-08-18 Affibody Ab Amyloid beta peptide binding polypeptide
CN101224414A (zh) * 2007-10-18 2008-07-23 西北大学 以四唑为功能基的多用途分离介质及其制备方法

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104513309A (zh) * 2013-09-26 2015-04-15 上海亨臻实业有限公司 一种蛋白功能结构域铰链肽及其用途
CN106117324A (zh) * 2016-07-29 2016-11-16 湖北爱晟生物科技有限公司 重组蛋白a及其编码基因和应用
CN111511467A (zh) * 2017-12-20 2020-08-07 通用电气医疗集团生物工艺研发股份公司 制备分离基质的方法
CN109721645A (zh) * 2017-12-29 2019-05-07 兆生生物科技南京有限公司 一种基因突变的蛋白a及其应用

Also Published As

Publication number Publication date
WO2012074463A1 (en) 2012-06-07
US8859726B2 (en) 2014-10-14
JP2014502272A (ja) 2014-01-30
US20130245224A1 (en) 2013-09-19
EP2646126A1 (en) 2013-10-09
EP2646126A4 (en) 2014-12-17

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Application publication date: 20130731