JP2014500729A5 - - Google Patents

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Publication number
JP2014500729A5
JP2014500729A5 JP2013543299A JP2013543299A JP2014500729A5 JP 2014500729 A5 JP2014500729 A5 JP 2014500729A5 JP 2013543299 A JP2013543299 A JP 2013543299A JP 2013543299 A JP2013543299 A JP 2013543299A JP 2014500729 A5 JP2014500729 A5 JP 2014500729A5
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JP
Japan
Prior art keywords
nucleic acid
target nucleic
inhibitor
detected
conjugated
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JP2013543299A
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English (en)
Japanese (ja)
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JP6096672B2 (ja
JP2014500729A (ja
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Priority claimed from PCT/US2011/063654 external-priority patent/WO2012078710A1/en
Publication of JP2014500729A publication Critical patent/JP2014500729A/ja
Publication of JP2014500729A5 publication Critical patent/JP2014500729A5/ja
Application granted granted Critical
Publication of JP6096672B2 publication Critical patent/JP6096672B2/ja
Expired - Fee Related legal-status Critical Current
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JP2013543299A 2010-12-07 2011-12-07 検出体、プローブ、および阻害剤を用いた核酸標的検出 Expired - Fee Related JP6096672B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US42074710P 2010-12-07 2010-12-07
US61/420,747 2010-12-07
PCT/US2011/063654 WO2012078710A1 (en) 2010-12-07 2011-12-07 Nucleic acid target detection using a detector, a probe and an inhibitor

Publications (3)

Publication Number Publication Date
JP2014500729A JP2014500729A (ja) 2014-01-16
JP2014500729A5 true JP2014500729A5 (enExample) 2015-01-29
JP6096672B2 JP6096672B2 (ja) 2017-03-15

Family

ID=46207491

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013543299A Expired - Fee Related JP6096672B2 (ja) 2010-12-07 2011-12-07 検出体、プローブ、および阻害剤を用いた核酸標的検出

Country Status (8)

Country Link
US (2) US9581549B2 (enExample)
EP (1) EP2649202B1 (enExample)
JP (1) JP6096672B2 (enExample)
CN (2) CN105603071B (enExample)
AU (1) AU2011338502B2 (enExample)
CA (1) CA2820094C (enExample)
SG (1) SG191725A1 (enExample)
WO (1) WO2012078710A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011338502B2 (en) * 2010-12-07 2016-08-11 Bio-Rad Laboratories, Inc. Nucleic acid target detection using a detector, a probe and an inhibitor
CA2841430C (en) 2011-03-30 2018-12-04 Gnubio, Inc. Injection of multiple volumes into or out of droplets
EP2691540B1 (en) 2011-03-31 2016-01-20 GnuBIO, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
US9228898B2 (en) 2011-03-31 2016-01-05 Gnubio, Inc. Scalable spectroscopic detection and measurement
CN108479872B (zh) 2012-09-12 2021-02-12 基纽拜奥股份有限公司 用于进行试验的集成微流体系统、方法和试剂盒
EP2948703B1 (en) 2013-01-25 2019-03-13 Bio-Rad Laboratories, Inc. System and method for performing droplet inflation
WO2014176599A1 (en) 2013-04-26 2014-10-30 Gnubio, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
EP3004813A4 (en) 2013-05-29 2016-12-21 Gnubio Inc COST-EFFECTIVE OPTICAL DISCRETE HIGH-SPEED MEASURING SYSTEM
WO2014194131A2 (en) * 2013-05-29 2014-12-04 Gnubio, Inc. Systems and methods for sequencing in emulsion based microfluidics
EP3039119A4 (en) 2013-08-27 2017-04-05 GnuBIO, Inc. Microfluidic devices and methods of their use
EP3052236B1 (en) 2013-09-30 2021-07-14 Bio-Rad Laboratories, Inc. Microfluidic cartridge device and methods of use and assembly
US10130950B2 (en) 2013-11-27 2018-11-20 Bio-Rad Laboratories, Inc. Microfluidic droplet packing
WO2016004022A2 (en) 2014-06-30 2016-01-07 Gnubio, Inc. Floating thermal contact enabled pcr
EP3574047B1 (en) 2017-01-30 2024-03-06 Bio-Rad Laboratories, Inc. Emulsion compositions and methods of their use
US10941445B2 (en) 2017-03-24 2021-03-09 Bio-Rad Laboratories, Inc. Universal hairpin primers

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US20030148303A1 (en) * 2000-09-19 2003-08-07 Nadeau James G. Universal probes and methods for detection of nucleic acids
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BRPI0414004A (pt) 2003-08-27 2006-10-24 Harvard College controle eletrÈnico de espécies fluìdicas
EP1984738A2 (en) 2006-01-11 2008-10-29 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
EP4190448A3 (en) * 2006-05-11 2023-09-20 Bio-Rad Laboratories, Inc. Microfluidic devices
EP2059614A4 (en) * 2006-08-15 2010-06-30 Zygene Biotech Llc ANTISONDON PROBE COMPOSITIONS AND METHODS OF DETECTION OF DNA OR RNA
US8568979B2 (en) * 2006-10-10 2013-10-29 Illumina, Inc. Compositions and methods for representational selection of nucleic acids from complex mixtures using hybridization
US20100129792A1 (en) 2007-02-06 2010-05-27 Gerassimos Makrigiorgos Direct monitoring and pcr amplification of the dosage and dosage difference between target genetic regions
JP2010528619A (ja) * 2007-05-31 2010-08-26 ベクトン・ディキンソン・アンド・カンパニー インフルエンザａおよびインフルエンザｂウイルスを検出するための配列および方法
US9797010B2 (en) 2007-12-21 2017-10-24 President And Fellows Of Harvard College Systems and methods for nucleic acid sequencing
CN102124259B (zh) 2008-05-16 2015-12-16 哈佛大学 在包含微流体系统的流体系统中的阀和其它流动控制
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3150724A1 (en) 2008-12-19 2017-04-05 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
JP5687414B2 (ja) * 2009-04-20 2015-03-18 オリンパス株式会社 多型の識別方法
EP4019977B1 (en) 2009-06-26 2024-11-20 President and Fellows of Harvard College Fluid injection
EP2493619B1 (en) 2009-10-27 2018-12-19 President and Fellows of Harvard College Droplet creation techniques
EP4435111A1 (en) 2010-02-12 2024-09-25 Bio-Rad Laboratories, Inc. Digital analyte analysis
AU2011338502B2 (en) 2010-12-07 2016-08-11 Bio-Rad Laboratories, Inc. Nucleic acid target detection using a detector, a probe and an inhibitor
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
CA2841430C (en) 2011-03-30 2018-12-04 Gnubio, Inc. Injection of multiple volumes into or out of droplets
EP2691540B1 (en) 2011-03-31 2016-01-20 GnuBIO, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
US9228898B2 (en) 2011-03-31 2016-01-05 Gnubio, Inc. Scalable spectroscopic detection and measurement
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
WO2013095737A2 (en) 2011-09-28 2013-06-27 President And Fellows Of Harvard College Systems and methods for droplet production and/or fluidic manipulation
US20150045258A1 (en) 2012-02-14 2015-02-12 Gnubio, Inc. Cascaded addition of target specific universal adapters to nucleic acids
WO2013165748A1 (en) 2012-04-30 2013-11-07 Raindance Technologies, Inc Digital analyte analysis
CN108479872B (zh) 2012-09-12 2021-02-12 基纽拜奥股份有限公司 用于进行试验的集成微流体系统、方法和试剂盒
CN105050583A (zh) 2012-12-14 2015-11-11 基纽拜奥股份有限公司 在乳液滴中维持分子的不均匀浓度的方法
EP2948703B1 (en) 2013-01-25 2019-03-13 Bio-Rad Laboratories, Inc. System and method for performing droplet inflation
WO2014176599A1 (en) 2013-04-26 2014-10-30 Gnubio, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
WO2014194131A2 (en) 2013-05-29 2014-12-04 Gnubio, Inc. Systems and methods for sequencing in emulsion based microfluidics
EP3004813A4 (en) 2013-05-29 2016-12-21 Gnubio Inc COST-EFFECTIVE OPTICAL DISCRETE HIGH-SPEED MEASURING SYSTEM
EP3039119A4 (en) 2013-08-27 2017-04-05 GnuBIO, Inc. Microfluidic devices and methods of their use
EP3052236B1 (en) 2013-09-30 2021-07-14 Bio-Rad Laboratories, Inc. Microfluidic cartridge device and methods of use and assembly
WO2016004022A2 (en) 2014-06-30 2016-01-07 Gnubio, Inc. Floating thermal contact enabled pcr

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