JP2016512041A5 - - Google Patents
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- Publication number
- JP2016512041A5 JP2016512041A5 JP2016502320A JP2016502320A JP2016512041A5 JP 2016512041 A5 JP2016512041 A5 JP 2016512041A5 JP 2016502320 A JP2016502320 A JP 2016502320A JP 2016502320 A JP2016502320 A JP 2016502320A JP 2016512041 A5 JP2016512041 A5 JP 2016512041A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- fluorophore
- nucleic acid
- quencher
- detectable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020004707 nucleic acids Proteins 0.000 claims 31
- 102000039446 nucleic acids Human genes 0.000 claims 31
- 150000007523 nucleic acids Chemical class 0.000 claims 31
- 239000000523 sample Substances 0.000 claims 20
- 108700028369 Alleles Proteins 0.000 claims 14
- 108091034117 Oligonucleotide Proteins 0.000 claims 13
- 238000000034 method Methods 0.000 claims 9
- 101150048834 braF gene Proteins 0.000 claims 8
- 101000984753 Homo sapiens Serine/threonine-protein kinase B-raf Proteins 0.000 claims 7
- 102100027103 Serine/threonine-protein kinase B-raf Human genes 0.000 claims 7
- 239000000203 mixture Substances 0.000 claims 7
- 238000001514 detection method Methods 0.000 claims 5
- 239000003153 chemical reaction reagent Substances 0.000 claims 4
- 230000000295 complement effect Effects 0.000 claims 4
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 3
- 238000010791 quenching Methods 0.000 claims 3
- 230000000171 quenching effect Effects 0.000 claims 3
- 108091093088 Amplicon Proteins 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 101100381978 Mus musculus Braf gene Proteins 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 238000007837 multiplex assay Methods 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 102000054765 polymorphisms of proteins Human genes 0.000 claims 1
- 239000011541 reaction mixture Substances 0.000 claims 1
- 238000006467 substitution reaction Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361792202P | 2013-03-15 | 2013-03-15 | |
| US61/792,202 | 2013-03-15 | ||
| PCT/US2014/027048 WO2014152185A1 (en) | 2013-03-15 | 2014-03-14 | Multiplex allele detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016512041A JP2016512041A (ja) | 2016-04-25 |
| JP2016512041A5 true JP2016512041A5 (enExample) | 2017-03-30 |
Family
ID=51529833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016502320A Pending JP2016512041A (ja) | 2013-03-15 | 2014-03-14 | 多重対立遺伝子の検出 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20140274779A1 (enExample) |
| EP (1) | EP2971147A4 (enExample) |
| JP (1) | JP2016512041A (enExample) |
| CN (1) | CN105431550A (enExample) |
| WO (1) | WO2014152185A1 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK3237636T3 (da) * | 2014-12-22 | 2021-01-25 | Anapa Biotech As | Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer |
| US20210108253A1 (en) * | 2017-03-30 | 2021-04-15 | Life Technologies Corporation | Quantification of ngs dna by adapter sequence |
| CN107760764B (zh) * | 2017-10-23 | 2023-04-07 | 上海阅尔基因技术有限公司 | 一种基于引物荧光和淬灭标记的靶核酸检测方法和试剂盒 |
| CN109355360A (zh) * | 2018-11-20 | 2019-02-19 | 元码基因科技（北京）股份有限公司 | 用于检测egfr突变的组合物、试剂盒和方法 |
| CN110195099B (zh) * | 2019-05-28 | 2021-07-13 | 西安交通大学 | 一种多靶标基因并行检测组合探针及其试剂盒的应用 |
| US20220017948A1 (en) * | 2020-06-23 | 2022-01-20 | Genomind, Inc. | Detection of specific hla alleles |
| JP7519537B2 (ja) * | 2020-09-23 | 2024-07-19 | マキュラ バイオテクノロジー カンパニー リミテッド | 核酸を検出する組み合わせ、方法及びキット |
| CN113201533B (zh) * | 2021-05-28 | 2023-06-20 | 南方医科大学 | 基于催化发夹自组装恒温扩增技术检测核酸的通用探针及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US6277607B1 (en) * | 1999-05-24 | 2001-08-21 | Sanjay Tyagi | High specificity primers, amplification methods and kits |
| US20030165859A1 (en) * | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| EP1509624A2 (en) * | 2002-05-31 | 2005-03-02 | Secretary, Department Of Atomic Energy | Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands |
| AU2005299590B2 (en) * | 2004-10-27 | 2010-09-16 | Cepheid | Closed-system multi-stage nucleic acid amplification reactions |
| JP5818018B2 (ja) * | 2009-03-26 | 2015-11-18 | 厦門艾徳生物医薬科技有限公司 | 核酸増幅を用いる環状プライマー及びその応用 |
| US8728763B2 (en) * | 2009-08-11 | 2014-05-20 | Response Genetics | Methods, primers, probes and kits useful for the detection of BRAF mutations |
| EP2464751B1 (en) * | 2009-08-11 | 2017-05-24 | Response Genetics, Inc. | Methods, primers, probes and kits useful for the detection of braf mutations |
| JP5822843B2 (ja) * | 2009-12-21 | 2015-11-24 | シージーン アイエヌシー | Ｔｓｇプライマーターゲット検出 |
| CN102161990A (zh) * | 2010-02-24 | 2011-08-24 | 北京雅康博生物科技有限公司 | 用于定量检测braf突变的试剂盒 |
-
2014
- 2014-03-14 WO PCT/US2014/027048 patent/WO2014152185A1/en not_active Ceased
- 2014-03-14 US US14/210,618 patent/US20140274779A1/en not_active Abandoned
- 2014-03-14 JP JP2016502320A patent/JP2016512041A/ja active Pending
- 2014-03-14 EP EP14768263.7A patent/EP2971147A4/en not_active Withdrawn
- 2014-03-14 CN CN201480028405.4A patent/CN105431550A/zh active Pending
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