JP2016512041A5 - - Google Patents
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- Publication number
- JP2016512041A5 JP2016512041A5 JP2016502320A JP2016502320A JP2016512041A5 JP 2016512041 A5 JP2016512041 A5 JP 2016512041A5 JP 2016502320 A JP2016502320 A JP 2016502320A JP 2016502320 A JP2016502320 A JP 2016502320A JP 2016512041 A5 JP2016512041 A5 JP 2016512041A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- fluorophore
- nucleic acid
- quencher
- detectable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020004707 nucleic acids Proteins 0.000 claims 31
- 102000039446 nucleic acids Human genes 0.000 claims 31
- 150000007523 nucleic acids Chemical class 0.000 claims 31
- 239000000523 sample Substances 0.000 claims 20
- 108700028369 Alleles Proteins 0.000 claims 14
- 108091034117 Oligonucleotide Proteins 0.000 claims 13
- 238000000034 method Methods 0.000 claims 9
- 101150048834 braF gene Proteins 0.000 claims 8
- 101000984753 Homo sapiens Serine/threonine-protein kinase B-raf Proteins 0.000 claims 7
- 102100027103 Serine/threonine-protein kinase B-raf Human genes 0.000 claims 7
- 239000000203 mixture Substances 0.000 claims 7
- 238000001514 detection method Methods 0.000 claims 5
- 239000003153 chemical reaction reagent Substances 0.000 claims 4
- 230000000295 complement effect Effects 0.000 claims 4
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 3
- 238000010791 quenching Methods 0.000 claims 3
- 230000000171 quenching effect Effects 0.000 claims 3
- 108091093088 Amplicon Proteins 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 101100381978 Mus musculus Braf gene Proteins 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 238000007837 multiplex assay Methods 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 102000054765 polymorphisms of proteins Human genes 0.000 claims 1
- 239000011541 reaction mixture Substances 0.000 claims 1
- 238000006467 substitution reaction Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361792202P | 2013-03-15 | 2013-03-15 | |
| US61/792,202 | 2013-03-15 | ||
| PCT/US2014/027048 WO2014152185A1 (en) | 2013-03-15 | 2014-03-14 | Multiplex allele detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016512041A JP2016512041A (ja) | 2016-04-25 |
| JP2016512041A5 true JP2016512041A5 (enExample) | 2017-03-30 |
Family
ID=51529833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016502320A Pending JP2016512041A (ja) | 2013-03-15 | 2014-03-14 | 多重対立遺伝子の検出 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20140274779A1 (enExample) |
| EP (1) | EP2971147A4 (enExample) |
| JP (1) | JP2016512041A (enExample) |
| CN (1) | CN105431550A (enExample) |
| WO (1) | WO2014152185A1 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018500932A (ja) * | 2014-12-22 | 2018-01-18 | アナーパ バイオテック エー／エス | 標的核酸のマルチプレックス検出のためのデュアルクエンチングアッセイ |
| WO2018183621A1 (en) * | 2017-03-30 | 2018-10-04 | Life Technologies Corporation | Quantification of ngs dna by adapter sequence |
| CN107760764B (zh) * | 2017-10-23 | 2023-04-07 | 上海阅尔基因技术有限公司 | 一种基于引物荧光和淬灭标记的靶核酸检测方法和试剂盒 |
| CN109355360A (zh) * | 2018-11-20 | 2019-02-19 | 元码基因科技（北京）股份有限公司 | 用于检测egfr突变的组合物、试剂盒和方法 |
| CN110195099B (zh) * | 2019-05-28 | 2021-07-13 | 西安交通大学 | 一种多靶标基因并行检测组合探针及其试剂盒的应用 |
| WO2021262589A1 (en) * | 2020-06-23 | 2021-12-30 | Genomind, Inc. | Detection of specific hla alleles |
| EP4219737A4 (en) * | 2020-09-23 | 2024-06-05 | Maccura Biotechnology Co., Ltd. | COMBINATION, METHOD AND KIT FOR DETECTION OF TARGET NUCLEIC ACID |
| CN113201533B (zh) * | 2021-05-28 | 2023-06-20 | 南方医科大学 | 基于催化发夹自组装恒温扩增技术检测核酸的通用探针及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US6277607B1 (en) * | 1999-05-24 | 2001-08-21 | Sanjay Tyagi | High specificity primers, amplification methods and kits |
| US20030165859A1 (en) * | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| WO2003102239A2 (en) * | 2002-05-31 | 2003-12-11 | Secretary, Department Of Atomic Energy | Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands |
| JP4999103B2 (ja) * | 2004-10-27 | 2012-08-15 | セフィード | 閉鎖系の多段階核酸増幅反応 |
| WO2010108325A1 (zh) * | 2009-03-26 | 2010-09-30 | 厦门艾德生物医药科技有限公司 | 一种用于核酸扩增的环形引物及其应用 |
| NZ598166A (en) * | 2009-08-11 | 2014-03-28 | Response Genetics Inc | Methods, primers, probes and kits useful for the detection of braf mutations |
| US8728763B2 (en) * | 2009-08-11 | 2014-05-20 | Response Genetics | Methods, primers, probes and kits useful for the detection of BRAF mutations |
| AU2010336137B2 (en) * | 2009-12-21 | 2015-08-06 | Seegene, Inc. | TSG primer target detection |
| CN102161990A (zh) * | 2010-02-24 | 2011-08-24 | 北京雅康博生物科技有限公司 | 用于定量检测braf突变的试剂盒 |
-
2014
- 2014-03-14 JP JP2016502320A patent/JP2016512041A/ja active Pending
- 2014-03-14 WO PCT/US2014/027048 patent/WO2014152185A1/en not_active Ceased
- 2014-03-14 EP EP14768263.7A patent/EP2971147A4/en not_active Withdrawn
- 2014-03-14 US US14/210,618 patent/US20140274779A1/en not_active Abandoned
- 2014-03-14 CN CN201480028405.4A patent/CN105431550A/zh active Pending
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