NZ598598A - Detection of aad-1 event das-40278-9 - Google Patents
Detection of aad-1 event das-40278-9Info
- Publication number
- NZ598598A NZ598598A NZ598598A NZ59859810A NZ598598A NZ 598598 A NZ598598 A NZ 598598A NZ 598598 A NZ598598 A NZ 598598A NZ 59859810 A NZ59859810 A NZ 59859810A NZ 598598 A NZ598598 A NZ 598598A
- Authority
- NZ
- New Zealand
- Prior art keywords
- event
- probe
- com
- primer
- florescent
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
Disclosed is a method for determining zygosity of a com plant comprising AAD-l com event DAS-40278-9, said event comprising the nucleotide sequence of SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises a trans gene construct comprising an AAD-l gene, said transgene construct being flanked by a 5’ flanking com genomic DNA and a 3’ flanking com genomic DNA, said method comprising: obtaining a DNA sample of genomic DNA from said com plant; producing a contacted sample by contacting said DNA sample with a. a first event primer and a second event primer, wherein said first event primer specifically binds said transgene construct, said second event primer specifically binds said 5’ com genomic flanking DNA or said 3’ com genomic flanking DNA, and wherein said first event primer and said second event primer produce an event amplicon when subjected to TAQMAN PCR conditions b. a reference forward primer and a reference reverse primer that produce a reference amplicon from an endogenous com reference gene when subjected to TAQMAN PCR conditions c. a florescent event probe that hybridizes with said event amplicon d. a florescent reference probe that hybridizes with said reference amplicon; subjecting said contacted sample to fluorescence-based endpoint TAQMAN PCR conditions; quantitating said florescent event probe that hybridized to said event amplicon; quantitating said florescent reference probe that hybridized to said reference amplicon; comparing amounts of hybridized florescent event probe to hybridized florescent reference probe; and determining zygosity of DAS-40278-9 by comparing florescence ratios of hybridized fluorescent event probe and hybridized fluorescent reference probe.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23524809P | 2009-08-19 | 2009-08-19 | |
| US23736610P | 2010-04-23 | 2010-04-23 | |
| PCT/US2010/045871 WO2011022471A1 (en) | 2009-08-19 | 2010-08-18 | Detection of aad-1 event das-40278-9 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ598598A true NZ598598A (en) | 2014-05-30 |
Family
ID=43607318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ598598A NZ598598A (en) | 2009-08-19 | 2010-08-18 | Detection of aad-1 event das-40278-9 |
Country Status (16)
| Country | Link |
|---|---|
| JP (1) | JP5771208B2 (en) |
| KR (1) | KR101772638B1 (en) |
| CN (1) | CN102575299B (en) |
| AU (1) | AU2010284286B2 (en) |
| BR (1) | BR112012003873A2 (en) |
| CA (1) | CA2771581C (en) |
| CL (1) | CL2012000410A1 (en) |
| CO (1) | CO6511214A2 (en) |
| HK (1) | HK1173194A1 (en) |
| IL (1) | IL218175A (en) |
| IN (1) | IN2012DN01800A (en) |
| MX (1) | MX336072B (en) |
| NZ (1) | NZ598598A (en) |
| RU (1) | RU2577143C2 (en) |
| WO (1) | WO2011022471A1 (en) |
| ZA (1) | ZA201201210B (en) |
Families Citing this family (58)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IN2012DN02294A (en) | 2009-08-19 | 2015-08-21 | Dow Agrosciences Llc | |
| US8598413B2 (en) * | 2009-08-19 | 2013-12-03 | Dow AgroSciecnes, LLC. | AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1 |
| TWI667347B (en) | 2010-12-15 | 2019-08-01 | 瑞士商先正達合夥公司 | Soybean event syht0h2 and compositions and methods for detection thereof |
| CN107072163A (en) * | 2014-03-20 | 2017-08-18 | 孟山都技术公司 | Transgenic corn events MON87419 and its application method |
| GB201622007D0 (en) | 2016-12-22 | 2017-02-08 | And See Cambridge Display Tech Ltd Syngenta Participations Ag | Polymorphs |
| MA47081B1 (en) | 2016-12-22 | 2021-04-30 | Syngenta Participations Ag | Polymorphs |
| UY37623A (en) | 2017-03-03 | 2018-09-28 | Syngenta Participations Ag | DERIVATIVES OF OXADIAZOL THIOPHEN FUNGICIDES |
| BR112019020134B1 (en) | 2017-03-31 | 2023-05-09 | Syngenta Participations Ag | FUNGICIDAL COMPOSITIONS |
| KR102615161B1 (en) | 2017-03-31 | 2023-12-15 | 신젠타 파티서페이션즈 아게 | fungicidal composition |
| CN110506040A (en) | 2017-04-03 | 2019-11-26 | 先正达参股股份有限公司 | Kill the oxadiazole derivatives of microorganism |
| WO2018184986A1 (en) | 2017-04-05 | 2018-10-11 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| WO2018184988A1 (en) | 2017-04-05 | 2018-10-11 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| BR112019020739B1 (en) | 2017-04-05 | 2023-12-19 | Syngenta Participations Ag | COMPOUNDS DERIVED FROM OXADIAZOLE MICROBIOCIDES AND THEIR USE, AGROCHEMICAL COMPOSITION, METHOD TO CONTROL OR PREVENT INFESTATION OF USEFUL PLANTS BY PHYTOPATHOGENIC MICROORGANISMS |
| WO2018184982A1 (en) | 2017-04-05 | 2018-10-11 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| WO2018184987A1 (en) | 2017-04-05 | 2018-10-11 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| WO2018184984A1 (en) | 2017-04-05 | 2018-10-11 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| WO2018185211A1 (en) | 2017-04-06 | 2018-10-11 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| WO2018219773A1 (en) | 2017-06-02 | 2018-12-06 | Syngenta Participations Ag | Fungicidal compositions |
| CN110709395A (en) | 2017-06-02 | 2020-01-17 | 先正达参股股份有限公司 | Microbicidal oxadiazole derivatives |
| BR112019026331A2 (en) | 2017-06-14 | 2020-07-21 | Syngenta Participations Ag | fungicidal compositions |
| BR112019027900A2 (en) | 2017-06-28 | 2020-07-21 | Syngenta Participations Ag | fungicidal compositions |
| WO2019011926A1 (en) | 2017-07-11 | 2019-01-17 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| WO2019011928A1 (en) | 2017-07-11 | 2019-01-17 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| WO2019011923A1 (en) | 2017-07-11 | 2019-01-17 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| BR112020000456A2 (en) | 2017-07-11 | 2020-07-21 | Syngenta Participations Ag | microbiocidal oxadiazole derivatives |
| BR112020000371A2 (en) | 2017-07-12 | 2020-07-14 | Syngenta Participations Ag | microbiocidal oxadiazole derivatives |
| WO2019012001A1 (en) | 2017-07-12 | 2019-01-17 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| WO2019012003A1 (en) | 2017-07-13 | 2019-01-17 | Syngenta Participations Ag | Microbiocidal oxadiazole derivatives |
| UY37913A (en) | 2017-10-05 | 2019-05-31 | Syngenta Participations Ag | PICOLINAMIDE DERIVATIVES FUNGICIDES THAT CARRY A QUATERNARY TERMINAL GROUP |
| UY37912A (en) | 2017-10-05 | 2019-05-31 | Syngenta Participations Ag | PICOLINAMIDE DERIVATIVES FUNGICIDES THAT CONTAIN HETEROARILO OR HETEROARILOXI TERMINAL GROUPS |
| US11291205B2 (en) | 2017-11-15 | 2022-04-05 | Syngenta Participations Ag | Microbiocidal picolinamide derivatives |
| CN112020503A (en) | 2018-04-26 | 2020-12-01 | 先正达参股股份有限公司 | Microbicidal oxadiazole derivatives |
| BR112020026877A2 (en) | 2018-06-29 | 2021-04-06 | Syngenta Crop Protection Ag | MICROBIOCIDAL OXADIAZOL DERIVATIVES |
| WO2020007658A1 (en) | 2018-07-02 | 2020-01-09 | Syngenta Crop Protection Ag | 3-(2-thienyl)-5-(trifluoromethyl)-1,2,4-oxadiazole derivatives as agrochemical fungicides |
| US20210267204A1 (en) | 2018-07-16 | 2021-09-02 | Syngenta Crop Protection Ag | Microbiocidal oxadiazole derivatives |
| CN113195462A (en) | 2018-10-17 | 2021-07-30 | 先正达农作物保护股份公司 | Microbicidal oxadiazole derivatives |
| AR116628A1 (en) | 2018-10-18 | 2021-05-26 | Syngenta Crop Protection Ag | MICROBIOCIDAL COMPOUNDS |
| WO2020165403A1 (en) | 2019-02-15 | 2020-08-20 | Syngenta Crop Protection Ag | Phenyl substituted thiazole derivatives as microbiocidal compounds |
| CA3129347A1 (en) | 2019-02-20 | 2020-08-27 | Syngenta Crop Protection Ag | Use of spiropidion |
| GB201903942D0 (en) | 2019-03-22 | 2019-05-08 | Syngenta Crop Protection Ag | Microbiocidal compounds |
| CN113966171A (en) | 2019-04-10 | 2022-01-21 | 先正达农作物保护股份公司 | Fungicidal compositions |
| WO2020208095A1 (en) | 2019-04-10 | 2020-10-15 | Syngenta Crop Protection Ag | Microbiocidal picolinamide derivatives |
| BR112021026861A2 (en) | 2019-07-05 | 2022-02-22 | Syngenta Crop Protection Ag | Microbiocidal picolinamide derivatives |
| GB201910037D0 (en) | 2019-07-12 | 2019-08-28 | Syngenta Crop Protection Ag | Microbiocidal compounds |
| US20230131427A1 (en) | 2020-03-05 | 2023-04-27 | Syngenta Crop Protection Ag | Fungicidal compositions |
| UY39115A (en) | 2020-03-05 | 2021-10-29 | Syngenta Crop Protection Ag | FUNGICIDE MIXTURES OF ARYL METHOXYACRYLATE DERIVATIVES |
| GB202006386D0 (en) | 2020-04-30 | 2020-06-17 | Syngenta Crop Protection Ag | Microbiocidal Compounds |
| GB202006399D0 (en) | 2020-04-30 | 2020-06-17 | Syngenta Crop Protection Ag | Microbiocidal compounds |
| GB202006480D0 (en) | 2020-05-01 | 2020-06-17 | Syngenta Crop Protection Ag | Microbiocidal compounds |
| GB202006606D0 (en) | 2020-05-05 | 2020-06-17 | Syngenta Crop Protection Ag | Microbiocidal compounds |
| GB202014840D0 (en) | 2020-09-21 | 2020-11-04 | Syngenta Crop Protection Ag | Microbiocidal compounds |
| KR102383881B1 (en) * | 2020-10-16 | 2022-04-06 | 국립생태원 | Simultaneous detection method for genetically modified maize |
| TW202231187A (en) | 2020-11-27 | 2022-08-16 | 瑞士商先正達農作物保護公司 | Pesticidal compositions |
| UY39544A (en) | 2020-12-02 | 2022-06-30 | Syngenta Crop Protection Ag | FUNGICIDE COMPOSITIONS COMPRISING A MIXTURE OF COMPONENTS (A) AND (B) AS ACTIVE PRINCIPLES |
| WO2022117650A1 (en) | 2020-12-02 | 2022-06-09 | Syngenta Crop Protection Ag | Fungicidal compositions |
| AR125089A1 (en) | 2021-03-19 | 2023-06-07 | Syngenta Crop Protection Ag | PESTICIDE COMPOSITIONS |
| WO2022233869A1 (en) | 2021-05-04 | 2022-11-10 | Syngenta Crop Protection Ag | Use of clethodim for insect control |
| CN117597025A (en) | 2021-07-02 | 2024-02-23 | 先正达农作物保护股份公司 | Use of haloxyfop-R-methyl for insect control |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6245974B1 (en) * | 1997-08-06 | 2001-06-12 | North Carolina State University | Matrix attachment regions |
| CL2003002461A1 (en) * | 2002-11-27 | 2005-01-07 | Dow Chemical Company Agroscien | IMMUNOGLOBULIN THAT UNDERSTANDS AT LEAST ONE AFUCOSILATED GLICAN, COMPOSITION THAT CONTAINS IT, NUCLEOTIDIC SEQUENCE AND VECTOR THAT UNDERSTANDS IT, PROCEDURE TO PRODUCE IMMUNOGLOBULIN SAID IN PLANTS. |
| MXPA06006729A (en) * | 2003-12-15 | 2006-08-18 | Monsanto Technology Llc | Corn plant mon88017 and compositions and methods for detection thereof. |
| SI2298901T2 (en) * | 2004-04-30 | 2017-08-31 | Dow Agrosciences Llc | Novel herbicide resistance genes |
| RU2270254C2 (en) * | 2004-04-30 | 2006-02-20 | Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук | Identification of transgenic dna sequences in plant material and products made of the same, oligonucleotide kit and bioarray therefor |
| WO2008143993A2 (en) * | 2007-05-17 | 2008-11-27 | Monsanto Technology Llc | Corn polymorphisms and methods of genotyping |
| IN2012DN02294A (en) * | 2009-08-19 | 2015-08-21 | Dow Agrosciences Llc | |
| US8598413B2 (en) * | 2009-08-19 | 2013-12-03 | Dow AgroSciecnes, LLC. | AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1 |
-
2010
- 2010-08-18 MX MX2012002076A patent/MX336072B/en unknown
- 2010-08-18 IN IN1800DEN2012 patent/IN2012DN01800A/en unknown
- 2010-08-18 WO PCT/US2010/045871 patent/WO2011022471A1/en active Application Filing
- 2010-08-18 NZ NZ598598A patent/NZ598598A/en not_active IP Right Cessation
- 2010-08-18 RU RU2012110253/10A patent/RU2577143C2/en not_active IP Right Cessation
- 2010-08-18 CN CN201080047115.6A patent/CN102575299B/en active Active
- 2010-08-18 CA CA2771581A patent/CA2771581C/en active Active
- 2010-08-18 BR BR112012003873A patent/BR112012003873A2/en not_active Application Discontinuation
- 2010-08-18 KR KR1020127006871A patent/KR101772638B1/en active IP Right Grant
- 2010-08-18 JP JP2012525662A patent/JP5771208B2/en not_active Expired - Fee Related
- 2010-08-18 AU AU2010284286A patent/AU2010284286B2/en not_active Ceased
-
2012
- 2012-02-16 IL IL218175A patent/IL218175A/en not_active IP Right Cessation
- 2012-02-16 CL CL2012000410A patent/CL2012000410A1/en unknown
- 2012-02-17 ZA ZA2012/01210A patent/ZA201201210B/en unknown
- 2012-03-06 CO CO12039002A patent/CO6511214A2/en not_active Application Discontinuation
-
2013
- 2013-01-11 HK HK13100530.2A patent/HK1173194A1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JP5771208B2 (en) | 2015-08-26 |
| KR20120053038A (en) | 2012-05-24 |
| WO2011022471A1 (en) | 2011-02-24 |
| MX336072B (en) | 2016-01-06 |
| IN2012DN01800A (en) | 2015-06-05 |
| CA2771581C (en) | 2018-05-01 |
| JP2013505706A (en) | 2013-02-21 |
| CL2012000410A1 (en) | 2012-10-19 |
| HK1173194A1 (en) | 2013-05-10 |
| BR112012003873A2 (en) | 2016-11-22 |
| RU2577143C2 (en) | 2016-03-10 |
| CO6511214A2 (en) | 2012-08-31 |
| KR101772638B1 (en) | 2017-08-31 |
| IL218175A0 (en) | 2012-07-31 |
| RU2012110253A (en) | 2013-09-27 |
| AU2010284286A1 (en) | 2012-03-29 |
| ZA201201210B (en) | 2012-10-31 |
| CN102575299A (en) | 2012-07-11 |
| CN102575299B (en) | 2015-09-09 |
| MX2012002076A (en) | 2012-03-21 |
| AU2010284286B2 (en) | 2015-08-20 |
| IL218175A (en) | 2017-04-30 |
| CA2771581A1 (en) | 2011-02-24 |
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