CN105431550A - 多重等位基因检测 - Google Patents

多重等位基因检测 Download PDF

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Publication number
CN105431550A
CN105431550A CN201480028405.4A CN201480028405A CN105431550A CN 105431550 A CN105431550 A CN 105431550A CN 201480028405 A CN201480028405 A CN 201480028405A CN 105431550 A CN105431550 A CN 105431550A
Authority
CN
China
Prior art keywords
primer
fluorophore
quencher
nucleic acid
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480028405.4A
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English (en)
Chinese (zh)
Inventor
S.黄
H.苏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Molecular Inc
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Abbott Molecular Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Molecular Inc filed Critical Abbott Molecular Inc
Publication of CN105431550A publication Critical patent/CN105431550A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201480028405.4A 2013-03-15 2014-03-14 多重等位基因检测 Pending CN105431550A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361792202P 2013-03-15 2013-03-15
US61/792202 2013-03-15
PCT/US2014/027048 WO2014152185A1 (en) 2013-03-15 2014-03-14 Multiplex allele detection

Publications (1)

Publication Number Publication Date
CN105431550A true CN105431550A (zh) 2016-03-23

Family

ID=51529833

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480028405.4A Pending CN105431550A (zh) 2013-03-15 2014-03-14 多重等位基因检测

Country Status (5)

Country Link
US (1) US20140274779A1 (enExample)
EP (1) EP2971147A4 (enExample)
JP (1) JP2016512041A (enExample)
CN (1) CN105431550A (enExample)
WO (1) WO2014152185A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107760764A (zh) * 2017-10-23 2018-03-06 上海阅尔基因技术有限公司 一种基于引物荧光和淬灭标记的靶核酸检测方法和试剂盒
CN109355360A (zh) * 2018-11-20 2019-02-19 元码基因科技（北京）股份有限公司 用于检测egfr突变的组合物、试剂盒和方法
CN110195099A (zh) * 2019-05-28 2019-09-03 西安交通大学 一种多靶标基因并行检测组合探针及其试剂盒的应用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2970966A1 (en) * 2014-12-22 2016-06-30 Anapa Biotech A/S Dual quenching assay for multiplex detection of target nucleic acids
EP3601608A1 (en) * 2017-03-30 2020-02-05 Life Technologies Corporation Quantification of ngs dna by adapter sequence
WO2021262589A1 (en) * 2020-06-23 2021-12-30 Genomind, Inc. Detection of specific hla alleles
KR20230066614A (ko) * 2020-09-23 2023-05-16 맥큐라 바이오테크놀로지 컴퍼니 리미티드 핵산 검출을 위한 조합물, 방법 및 키트
CN113201533B (zh) * 2021-05-28 2023-06-20 南方医科大学 基于催化发夹自组装恒温扩增技术检测核酸的通用探针及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110269124A1 (en) * 2009-08-11 2011-11-03 Response Genetics Methods, Primers, Probes and Kits Useful for the Detection of BRAF Mutations
CN102762744A (zh) * 2009-12-21 2012-10-31 Seegene株式会社 利用靶信号产生引物进行的靶检测

Family Cites Families (8)

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US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6277607B1 (en) * 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
US20030165859A1 (en) * 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US20070059690A1 (en) * 2002-05-31 2007-03-15 Amirul Islam "Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands"
WO2006047777A2 (en) * 2004-10-27 2006-05-04 Cepheid Closed-system multi-stage nucleic acid amplification reactions
DK2412718T3 (en) * 2009-03-26 2017-01-30 Xiamen Amoy Diagnostics Co Ltd CIRCUIT PRIMES USED BY NUCLEIC ACID AMPLIFICATION AND USE THEREOF
JP2013501523A (ja) * 2009-08-11 2013-01-17 レスポンス ジェネティクス，インコーポレイティド Ｂｒａｆ変異の検出に有用な方法、プライマー、プローブ、及びキット
CN102161990A (zh) * 2010-02-24 2011-08-24 北京雅康博生物科技有限公司 用于定量检测braf突变的试剂盒

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110269124A1 (en) * 2009-08-11 2011-11-03 Response Genetics Methods, Primers, Probes and Kits Useful for the Detection of BRAF Mutations
CN102762744A (zh) * 2009-12-21 2012-10-31 Seegene株式会社 利用靶信号产生引物进行的靶检测

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HAZBON ET AL: "Harpin Primers for Simplified Single-Nucleotide Polymorphism Analysis of Mycobacterium tuberculosis and Other Organisms", 《JOURNAL OF CLINICAL MICROBIOLOGY》 *
WHITCOMBE ET AL: "Detection of PCR products using self-probing amplicons and fluorescence", 《NATURE BIOTECHNOLOGY》 *
司徒博 等: "利用等位基因特异性扩增检测人结直肠癌BRAF基因V600E突变", 《中国实验诊断学》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107760764A (zh) * 2017-10-23 2018-03-06 上海阅尔基因技术有限公司 一种基于引物荧光和淬灭标记的靶核酸检测方法和试剂盒
CN107760764B (zh) * 2017-10-23 2023-04-07 上海阅尔基因技术有限公司 一种基于引物荧光和淬灭标记的靶核酸检测方法和试剂盒
CN109355360A (zh) * 2018-11-20 2019-02-19 元码基因科技（北京）股份有限公司 用于检测egfr突变的组合物、试剂盒和方法
CN110195099A (zh) * 2019-05-28 2019-09-03 西安交通大学 一种多靶标基因并行检测组合探针及其试剂盒的应用

Also Published As

Publication number Publication date
WO2014152185A1 (en) 2014-09-25
US20140274779A1 (en) 2014-09-18
JP2016512041A (ja) 2016-04-25
EP2971147A4 (en) 2016-08-31
EP2971147A1 (en) 2016-01-20

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Application publication date: 20160323