JP2014500729A5 - - Google Patents
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- JP2014500729A5 JP2014500729A5 JP2013543299A JP2013543299A JP2014500729A5 JP 2014500729 A5 JP2014500729 A5 JP 2014500729A5 JP 2013543299 A JP2013543299 A JP 2013543299A JP 2013543299 A JP2013543299 A JP 2013543299A JP 2014500729 A5 JP2014500729 A5 JP 2014500729A5
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- JP
- Japan
- Prior art keywords
- nucleic acid
- target nucleic
- inhibitor
- detected
- conjugated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Claims (19)
- 標的核酸を検出するための方法であって、
a)標的核酸に検出体を組み込むことと、
b)反応物にオリゴヌクレオチドプローブを添加することと、
c)前記反応物に阻害剤を添加することと、
d)前記標的核酸の存在の指標として前記オリゴヌクレオチドプローブの前記阻害剤との干渉を検出することと、を含む、方法。 - 前記標的核酸が一本鎖核酸である、請求項1に記載の方法。
- 前記標的核酸内の単一ヌクレオチドが検出される、請求項1に記載の方法。
- 前記標的核酸内のヌクレオチドの短配列が検出される、請求項1に記載の方法。
- 前記標的核酸全体が検出される、請求項1に記載の方法。
- 前記標的核酸内の変異が検出される、請求項1に記載の方法。
- 前記標的核酸内の突然変異が検出される、請求項1に記載の方法。
- 前記標的核酸内の多型が検出される、請求項1に記載の方法。
- 前記検出体がフルオロフォアに共役される、請求項1に記載の方法。
- 前記阻害剤が消光剤に共役される、請求項1に記載の方法。
- 前記検出体が消光剤に共役される、請求項1に記載の方法。
- 前記阻害剤がフルオロフォアに共役される、請求項1に記載の方法。
- 前記検出体がエピトープに共役される、請求項1に記載の方法。
- 前記阻害剤がエピトープに共役される、請求項1に記載の方法。
- 1つを超える標的核酸が検出される、請求項1に記載の方法。
- 前記方法が乳剤内で行われる、請求項1〜15のいずれか一項に記載の方法。
- 前記乳剤がマイクロ流体デバイス内にある、請求項16に記載の方法。
- 請求項1〜17のいずれか一項に記載の方法を実施するためのキット。
- 前記阻害剤が、オリゴヌクレオチド阻害剤であり、前記検出は、前記オリゴヌクレオチドプローブが前記標的核酸にハイブリダイズする場合、前記オリゴヌクレオチド阻害剤を置換する、ポリメラーゼによる鎖置換反応を行うことを含む、請求項1に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US42074710P | 2010-12-07 | 2010-12-07 | |
US61/420,747 | 2010-12-07 | ||
PCT/US2011/063654 WO2012078710A1 (en) | 2010-12-07 | 2011-12-07 | Nucleic acid target detection using a detector, a probe and an inhibitor |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2014500729A JP2014500729A (ja) | 2014-01-16 |
JP2014500729A5 true JP2014500729A5 (ja) | 2015-01-29 |
JP6096672B2 JP6096672B2 (ja) | 2017-03-15 |
Family
ID=46207491
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2013543299A Active JP6096672B2 (ja) | 2010-12-07 | 2011-12-07 | 検出体、プローブ、および阻害剤を用いた核酸標的検出 |
Country Status (8)
Country | Link |
---|---|
US (2) | US9581549B2 (ja) |
EP (1) | EP2649202B1 (ja) |
JP (1) | JP6096672B2 (ja) |
CN (2) | CN105603071B (ja) |
AU (1) | AU2011338502B2 (ja) |
CA (1) | CA2820094C (ja) |
SG (1) | SG191725A1 (ja) |
WO (1) | WO2012078710A1 (ja) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
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AU2011338502B2 (en) | 2010-12-07 | 2016-08-11 | Bio-Rad Laboratories, Inc. | Nucleic acid target detection using a detector, a probe and an inhibitor |
CN103765068B (zh) | 2011-03-30 | 2016-09-07 | 努拜欧有限公司 | 将多个体积注入或注出液滴 |
JP5986623B2 (ja) | 2011-03-31 | 2016-09-06 | ヌビオ,インコーポレイテッド | スケーラブルなスペクトル検出および計測 |
US9816931B2 (en) | 2011-03-31 | 2017-11-14 | Bio-Rad Laboratories, Inc. | Managing variation in spectroscopic intensity measurements through the use of a reference component |
CN104781386B (zh) | 2012-09-12 | 2018-04-06 | 基纽拜奥股份有限公司 | 用于进行试验的集成微流体系统、方法和试剂盒 |
US9592503B2 (en) | 2013-01-25 | 2017-03-14 | Gnubio, Inc. | System and method for performing droplet inflation |
WO2014176599A1 (en) | 2013-04-26 | 2014-10-30 | Gnubio, Inc. | A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure |
CN105393094B (zh) | 2013-05-29 | 2019-07-23 | 生物辐射实验室股份有限公司 | 低成本光学高速离散测量系统 |
US9809851B2 (en) | 2013-05-29 | 2017-11-07 | Bio-Rad Laboratories, Inc. | Systems and methods for sequencing in emulsion based microfluidics |
CN105765055A (zh) | 2013-08-27 | 2016-07-13 | 基纽拜奥股份有限公司 | 微流体装置和其使用方法 |
WO2015048798A1 (en) | 2013-09-30 | 2015-04-02 | Gnubio, Inc. | Microfluidic cartridge device and methods of use and assembly |
US10130950B2 (en) | 2013-11-27 | 2018-11-20 | Bio-Rad Laboratories, Inc. | Microfluidic droplet packing |
CN106573245B (zh) | 2014-06-30 | 2019-06-18 | 生物辐射实验室股份有限公司 | 实现pcr的浮动热接触 |
US11118217B2 (en) | 2017-01-30 | 2021-09-14 | Bio-Rad Laboratories, Inc. | Emulsion compositions and methods of their use |
CN110446787A (zh) | 2017-03-24 | 2019-11-12 | 生物辐射实验室股份有限公司 | 通用发夹引物 |
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CN1842368B (zh) | 2003-08-27 | 2014-05-28 | 哈佛大学 | 流体物种的电子控制 |
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AU2011338502B2 (en) | 2010-12-07 | 2016-08-11 | Bio-Rad Laboratories, Inc. | Nucleic acid target detection using a detector, a probe and an inhibitor |
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US9150852B2 (en) | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
CN103765068B (zh) | 2011-03-30 | 2016-09-07 | 努拜欧有限公司 | 将多个体积注入或注出液滴 |
US9816931B2 (en) | 2011-03-31 | 2017-11-14 | Bio-Rad Laboratories, Inc. | Managing variation in spectroscopic intensity measurements through the use of a reference component |
JP5986623B2 (ja) | 2011-03-31 | 2016-09-06 | ヌビオ,インコーポレイテッド | スケーラブルなスペクトル検出および計測 |
US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
CN106423314B (zh) | 2011-09-28 | 2021-03-02 | 哈佛学院院长等 | 用于液滴产生和/或流体操纵的系统和方法 |
WO2013122826A1 (en) | 2012-02-14 | 2013-08-22 | Gnubio, Inc. | Cascaded addition of target specific universal adapters to nucleic acids |
EP3524693A1 (en) | 2012-04-30 | 2019-08-14 | Raindance Technologies, Inc. | Digital analyte analysis |
CN104781386B (zh) | 2012-09-12 | 2018-04-06 | 基纽拜奥股份有限公司 | 用于进行试验的集成微流体系统、方法和试剂盒 |
WO2014093976A1 (en) | 2012-12-14 | 2014-06-19 | Gnubio, Inc. | Method for maintaining heterogeneous concentrations of molecules in emulsion droplets |
US9592503B2 (en) | 2013-01-25 | 2017-03-14 | Gnubio, Inc. | System and method for performing droplet inflation |
WO2014176599A1 (en) | 2013-04-26 | 2014-10-30 | Gnubio, Inc. | A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure |
CN105393094B (zh) | 2013-05-29 | 2019-07-23 | 生物辐射实验室股份有限公司 | 低成本光学高速离散测量系统 |
US9809851B2 (en) | 2013-05-29 | 2017-11-07 | Bio-Rad Laboratories, Inc. | Systems and methods for sequencing in emulsion based microfluidics |
CN105765055A (zh) | 2013-08-27 | 2016-07-13 | 基纽拜奥股份有限公司 | 微流体装置和其使用方法 |
WO2015048798A1 (en) | 2013-09-30 | 2015-04-02 | Gnubio, Inc. | Microfluidic cartridge device and methods of use and assembly |
CN106573245B (zh) | 2014-06-30 | 2019-06-18 | 生物辐射实验室股份有限公司 | 实现pcr的浮动热接触 |
-
2011
- 2011-12-07 AU AU2011338502A patent/AU2011338502B2/en active Active
- 2011-12-07 JP JP2013543299A patent/JP6096672B2/ja active Active
- 2011-12-07 US US13/992,187 patent/US9581549B2/en active Active
- 2011-12-07 WO PCT/US2011/063654 patent/WO2012078710A1/en active Application Filing
- 2011-12-07 CN CN201610045852.1A patent/CN105603071B/zh active Active
- 2011-12-07 EP EP11846231.6A patent/EP2649202B1/en active Active
- 2011-12-07 CN CN201180058333.4A patent/CN103348017B/zh active Active
- 2011-12-07 CA CA2820094A patent/CA2820094C/en active Active
- 2011-12-07 SG SG2013043880A patent/SG191725A1/en unknown
-
2017
- 2017-01-17 US US15/408,191 patent/US10533212B2/en active Active
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