JP5986623B2 - スケーラブルなスペクトル検出および計測 - Google Patents
スケーラブルなスペクトル検出および計測 Download PDFInfo
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- JP5986623B2 JP5986623B2 JP2014502715A JP2014502715A JP5986623B2 JP 5986623 B2 JP5986623 B2 JP 5986623B2 JP 2014502715 A JP2014502715 A JP 2014502715A JP 2014502715 A JP2014502715 A JP 2014502715A JP 5986623 B2 JP5986623 B2 JP 5986623B2
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- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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Description
本願は、2011年3月31日に出願された、米国仮特許出願整理番号第61/469,889号の優先権を主張するものである。
実施例1
図6は本発明にしたがって構築された検出システムの実施形態を用いて取得された読み出しの例を示す。液滴信号701を包囲するボックスにより示される蛍光標識された試料を含有する1つの特定の液滴からの信号に対応するデジタル化されたレコード700の1部分が示される。検出器出力は、デジタル化されたレコード700において、最も古いデータが底部に最も新しいデータが上部にある状態で垂直にスタックされた配列として示される、強度が変化する画素のラインを含む。下方には、デジタル化されたレコード700が示される。なお、グラフ720は液滴信号701の数学的な成分分解の結果を示すものである。このグラフ上の垂直強度軸は液滴信号701から記録された強度に直接対応し、水平軸は液滴信号701における画素数に直接対応する。全体信号721は以下の成分、すなわち入射光による励起時にマイクロ流体チャネルを構成する物質により放出される自家蛍光成分725と、第1蛍光染料成分726と、第2蛍光染料成分727と、を含む。これら3つの成分725〜727を加え合わせると全体信号721が形成され得る。数学的な成分分解は、曲線725〜727により示される自家蛍光および既知の染料濃度に対する基準応答モデルの所望により重みが加えられた組み合わせを決定し、それにより観察された全体信号721が生成される。これらの基準応答モデルが、単一の実行されたデジタル化されたレコード700内で見出される観察を用いて確立され、応答成分に対する別個に準備された応答を用いて確立されない場合は、画素数と波長との間に正確な対応関係を確立する必要はない。
図7は、本発明にしたがって構築された検出システムの、実施例1に対して用いられたのと同じ実施形態を用いて取得されたデジタル化されたレコードの第2の例を示す。この事例において、対象となる試料は、一定組成の単一染料溶液の単相流である。結果として生じた、記録されたスペクトル信号は実質的に時間に対して不変である。
Claims (31)
- 試料からの光のスペクトル特性を検出および計測するためのシステムであって、前記システムは、
複数のマイクロ流体チャネルを有するマイクロ流体ハウジングであって、前記マイクロ流体チャネルは、前記試料のスケーラブルな流体流れに対応することができるように構成される、マイクロ流体ハウジングと、
単一の分散要素を備える光学トレインと、
撮像センサと、を備え、
各マイクロ流体チャネル内にある前記試料の流体流れからの光は、前記単一の分散要素によりスペクトル分散され、前記光のスペクトル特性は、前記試料と前記撮像センサとの間の相対運動の関数として前記撮像センサ上である期間にわたって計測され、さらに、重ね合わされた光の計測結果は、前記試料中の複数の構成要素の正体および濃度を判定するために数学的に成分分解される、システム。 - 前記光は、試料の照射後に試料から散乱された光、前記試料中の化学的プロセスによる化学発光として放出される光、または前記試料にめがけた広帯域光源の指向の後における光の選択的吸収の結果として前記試料を通して透過された光、1つ以上の蛍光標識を含む試料の励起後に蛍光として放出される光、およびそれらの組み合わせを含む群から選択される、請求項1に記載のシステム。
- 前記試料は光源により励起され、前記光源は、レーザ、発光ダイオード(LED)、アークランプ、高輝度電球、およびそれらの組み合わせを含む群から選択される、請求項1に記載のシステム。
- 前記試料は単相流および液滴のどちらかまたは両方を含む、請求項1に記載のシステム。
- 前記試料は、不連続的なターゲット、ビーズを含む不連続的なターゲット、細胞を含む不連続的なターゲット、およびそれらの組み合わせを含む、請求項1に記載のシステム。
- 前記試料はエマルジョン内に存在する、請求項1に記載のシステム。
- 前記試料はマイクロ流体装置内のエマルジョン内に存在する、請求項6に記載のシステム。
- 前記光のスペクトル特性は波長および/または強度を含む、請求項1に記載のシステム。
- 前記撮像センサは、単一のモノクローム光センサ、光センサの画素アレイ、1次元(ライン)における光センサの画素アレイ、2次元(グリッド)における光センサの画素アレイ、時間遅延積分(TDI)センサ、およびそれらの組み合わせを含む群から選択される、請求項1に記載のシステム。
- 前記撮像センサがTDIセンサである場合、ある期間にわたる光の計測結果は、連続的な時間的期間の間に前記TDIセンサ上で重ね合わされる、請求項9に記載のシステム。
- 前記単一の分散要素は、回折格子、平面透過格子、平面反射格子、分散プリズム、およびゼロ偏角(直視)プリズム装置を含む群から選択される、請求項1に記載のシステム。
- 前記試料の流れを観察し、且つ落射照明位置から前記試料を照明する監視光源システムを有する監視カメラシステムをさらに備える、請求項1に記載のシステム。
- 前記試料は前記監視光源システムにより励起される、請求項12に記載のシステム。
- 前記監視カメラシステムは、高速流動する液滴または粒子を撮像するためにストロボ発光される、請求項12に記載のシステム。
- 前記複数のマイクロ流体チャネルは並列に配列される、請求項1に記載のシステム。
- 複数の試料の中に存在する各試料からの光のスペクトル特性をスケーラブルに、同時に、検出および計測するためのシステムであって、前記システムは、
複数のマイクロ流体チャネルを有するマイクロ流体ハウジングであって、前記マイクロ流体チャネルは、前記試料の流体流れに対応することができるように構成される、マイクロ流体ハウジングと、
単一の分散要素を備える光学トレインと、
撮像センサと、を備え、
各マイクロ流体チャネル内にある前記試料の各流体流れからの光は、前記単一の分散要素によりスペクトル分散され、前記光のスペクトル特性は、各特定の試料と前記撮像センサとの間の相対運動の関数として前記撮像センサ上である期間にわたって計測され、さらに、重ね合わされた光の計測結果は、前記試料中の複数の構成要素の正体および濃度を判定するために数学的に成分分解される、システム。 - 前記光は、試料の照射後に前記試料から散乱された光、前記試料中の化学的プロセスによる化学発光として放出される光、または前記試料にめがけた広帯域光源の指向の後における光の選択的吸収の結果として前記試料を通して透過された光、1つ以上の蛍光標識を含む試料の励起後に蛍光として放出される光、およびそれらの組み合わせを含む群から選択される、請求項16に記載のシステム。
- 前記試料は光源により励起され、前記光源は、レーザ、発光ダイオード(LED)、アークランプ、高輝度電球、およびそれらの組み合わせを含む群から選択される、請求項16に記載のシステム。
- 前記複数の試料のそれぞれは単相流および液滴のどちらかまたは両方を含む、請求項16に記載のシステム。
- 前記複数の試料のそれぞれは、不連続的なターゲット、ビーズを含む不連続的なターゲット、細胞を含む不連続的なターゲット、およびそれらの組み合わせを含む群から選択される、請求項16に記載のシステム。
- 前記複数の試料はエマルジョン内に存在する、請求項16に記載のシステム。
- 前記複数の試料はマイクロ流体装置内のエマルジョン内に存在する、請求項21に記載のシステム。
- 前記光のスペクトル特性は波長および/または強度を含む、請求項16に記載のシステム。
- 前記撮像センサは、光センサの画素アレイ、光センサの画素アレイ、1次元(ライン)における光センサの画素アレイ、2次元(グリッド)における光センサの画素アレイ、時間遅延積分(TDI)センサ、およびそれらの組み合わせを含む群から選択される、請求項16に記載のシステム。
- 前記撮像センサがTDIセンサである場合、ある期間にわたる光の計測結果は、連続的な時間的期間の間に前記TDIセンサ上で重ね合わされる、請求項24に記載のシステム。
- 前記単一の分散要素は、回折格子、平面透過格子、平面反射格子、分散プリズム、およびゼロ偏角(直視)プリズム装置を含む群から選択される、請求項16に記載のシステム。
- 前記試料の各流れを観察し、且つ落射照明位置から各試料を照明する監視光源システムを有する監視カメラシステムをさらに備える、請求項16に記載のシステム。
- 前記試料は前記監視光源システムにより励起される、請求項27に記載のシステム。
- 前記監視カメラシステムは、高速流動する液滴または粒子を撮像するためにストロボ発光される、請求項27に記載のシステム。
- 前記複数の試料のそれぞれは、前記撮像センサの別個の領域上に投影される撮像範囲を有する、請求項16に記載のシステム。
- 前記複数のマイクロ流体チャネルは並列に配列される、請求項16に記載のシステム。
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US201161469889P | 2011-03-31 | 2011-03-31 | |
US61/469,889 | 2011-03-31 | ||
PCT/US2012/030716 WO2012135201A1 (en) | 2011-03-31 | 2012-03-27 | Scalable spectroscopic detection and measurement |
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