CA2820094C - Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides - Google Patents

Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides Download PDF

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Publication number
CA2820094C
CA2820094C CA2820094A CA2820094A CA2820094C CA 2820094 C CA2820094 C CA 2820094C CA 2820094 A CA2820094 A CA 2820094A CA 2820094 A CA2820094 A CA 2820094A CA 2820094 C CA2820094 C CA 2820094C
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Prior art keywords
nucleic acid
fluorophore
inhibitor
target nucleic
oligonucleotide
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CA2820094A
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English (en)
French (fr)
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CA2820094A1 (en
Inventor
Tal Raz
Pascaline Mary
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Dispersion Chemistry (AREA)
CA2820094A 2010-12-07 2011-12-07 Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides Active CA2820094C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US42074710P 2010-12-07 2010-12-07
US61/420,747 2010-12-07
PCT/US2011/063654 WO2012078710A1 (en) 2010-12-07 2011-12-07 Nucleic acid target detection using a detector, a probe and an inhibitor

Publications (2)

Publication Number Publication Date
CA2820094A1 CA2820094A1 (en) 2012-06-14
CA2820094C true CA2820094C (en) 2019-02-26

Family

ID=46207491

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2820094A Active CA2820094C (en) 2010-12-07 2011-12-07 Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides

Country Status (8)

Country Link
US (2) US9581549B2 (enExample)
EP (1) EP2649202B1 (enExample)
JP (1) JP6096672B2 (enExample)
CN (2) CN105603071B (enExample)
AU (1) AU2011338502B2 (enExample)
CA (1) CA2820094C (enExample)
SG (1) SG191725A1 (enExample)
WO (1) WO2012078710A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2820094C (en) 2010-12-07 2019-02-26 Gnubio, Inc. Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides
JP6472998B2 (ja) 2011-03-30 2019-02-20 バイオ−ラッド ラボラトリーズ インコーポレイテッド 液滴内への、または液滴からの複数の容量の注入
EP2691540B1 (en) 2011-03-31 2016-01-20 GnuBIO, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
WO2012135201A1 (en) 2011-03-31 2012-10-04 Gnubio, Inc. Scalable spectroscopic detection and measurement
US9821312B2 (en) 2012-09-12 2017-11-21 Bio-Rad Laboratories, Inc. Integrated microfluidic system, method and kit for performing assays
US9592503B2 (en) 2013-01-25 2017-03-14 Gnubio, Inc. System and method for performing droplet inflation
EP2989213B1 (en) 2013-04-26 2019-11-06 Bio-Rad Laboratories, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
EP3004813A4 (en) 2013-05-29 2016-12-21 Gnubio Inc COST-EFFECTIVE OPTICAL DISCRETE HIGH-SPEED MEASURING SYSTEM
EP3004391B1 (en) 2013-05-29 2019-03-27 Bio-Rad Laboratories, Inc. Methods for sequencing in emulsion based microfluidics
CN105765055A (zh) 2013-08-27 2016-07-13 基纽拜奥股份有限公司 微流体装置和其使用方法
EP3052236B1 (en) 2013-09-30 2021-07-14 Bio-Rad Laboratories, Inc. Microfluidic cartridge device and methods of use and assembly
US10130950B2 (en) 2013-11-27 2018-11-20 Bio-Rad Laboratories, Inc. Microfluidic droplet packing
US9683792B2 (en) 2014-06-30 2017-06-20 Bio-Rad Laboratories, Inc. Floating thermal contact enabled PCR
US11118217B2 (en) 2017-01-30 2021-09-14 Bio-Rad Laboratories, Inc. Emulsion compositions and methods of their use
WO2018175399A1 (en) 2017-03-24 2018-09-27 Bio-Rad Laboratories, Inc. Universal hairpin primers

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6007994A (en) * 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization
US6235502B1 (en) * 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
US20030148303A1 (en) * 2000-09-19 2003-08-07 Nadeau James G. Universal probes and methods for detection of nucleic acids
JP4537053B2 (ja) * 2001-06-25 2010-09-01 ジョージア テック リサーチ コーポレーション 二重共鳴エネルギー転移核酸プローブ
EP2662136A3 (en) 2003-08-27 2013-12-25 President and Fellows of Harvard College Method for handling and mixing droplets
EP3913375A1 (en) 2006-01-11 2021-11-24 Bio-Rad Laboratories, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
EP2021113A2 (en) * 2006-05-11 2009-02-11 Raindance Technologies, Inc. Microfluidic devices
WO2008021446A2 (en) * 2006-08-15 2008-02-21 Genetag Technology, Inc. Probe-antiprobe compositions and methods for dna or rna detection
US8568979B2 (en) * 2006-10-10 2013-10-29 Illumina, Inc. Compositions and methods for representational selection of nucleic acids from complex mixtures using hybridization
US20100129792A1 (en) * 2007-02-06 2010-05-27 Gerassimos Makrigiorgos Direct monitoring and pcr amplification of the dosage and dosage difference between target genetic regions
EP2152871A4 (en) * 2007-05-31 2011-01-05 Becton Dickinson Co SEQUENCES AND METHOD FOR DETECTING INFLUENZA VIRUS A AND INFLUENZA VIRUS B
US9797010B2 (en) 2007-12-21 2017-10-24 President And Fellows Of Harvard College Systems and methods for nucleic acid sequencing
EP2286125B1 (en) 2008-05-16 2015-07-08 President and Fellows of Harvard College Valves and other flow control in fluidic systems including microfluidic systems
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3290531B1 (en) 2008-12-19 2019-07-24 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
JP5687414B2 (ja) * 2009-04-20 2015-03-18 オリンパス株式会社 多型の識別方法
JP5841937B2 (ja) 2009-06-26 2016-01-13 プレジデント アンド フェローズ オブ ハーバード カレッジ 流体注入
EP2493619B1 (en) 2009-10-27 2018-12-19 President and Fellows of Harvard College Droplet creation techniques
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
CA2820094C (en) 2010-12-07 2019-02-26 Gnubio, Inc. Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
JP6472998B2 (ja) 2011-03-30 2019-02-20 バイオ−ラッド ラボラトリーズ インコーポレイテッド 液滴内への、または液滴からの複数の容量の注入
EP2691540B1 (en) 2011-03-31 2016-01-20 GnuBIO, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
WO2012135201A1 (en) 2011-03-31 2012-10-04 Gnubio, Inc. Scalable spectroscopic detection and measurement
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US11389800B2 (en) 2011-09-28 2022-07-19 President And Fellows Of Harvard College Systems and methods for droplet production and/or fluidic manipulation
US20150045258A1 (en) 2012-02-14 2015-02-12 Gnubio, Inc. Cascaded addition of target specific universal adapters to nucleic acids
WO2013165748A1 (en) 2012-04-30 2013-11-07 Raindance Technologies, Inc Digital analyte analysis
US9821312B2 (en) 2012-09-12 2017-11-21 Bio-Rad Laboratories, Inc. Integrated microfluidic system, method and kit for performing assays
US20150321163A1 (en) 2012-12-14 2015-11-12 Gnubio, Inc. Method for maintaining heterogeneous concentrations of molecules in emulsion droplets
US9592503B2 (en) 2013-01-25 2017-03-14 Gnubio, Inc. System and method for performing droplet inflation
EP2989213B1 (en) 2013-04-26 2019-11-06 Bio-Rad Laboratories, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
EP3004391B1 (en) 2013-05-29 2019-03-27 Bio-Rad Laboratories, Inc. Methods for sequencing in emulsion based microfluidics
EP3004813A4 (en) 2013-05-29 2016-12-21 Gnubio Inc COST-EFFECTIVE OPTICAL DISCRETE HIGH-SPEED MEASURING SYSTEM
CN105765055A (zh) 2013-08-27 2016-07-13 基纽拜奥股份有限公司 微流体装置和其使用方法
EP3052236B1 (en) 2013-09-30 2021-07-14 Bio-Rad Laboratories, Inc. Microfluidic cartridge device and methods of use and assembly
US9683792B2 (en) 2014-06-30 2017-06-20 Bio-Rad Laboratories, Inc. Floating thermal contact enabled PCR

Also Published As

Publication number Publication date
EP2649202B1 (en) 2019-02-20
CA2820094A1 (en) 2012-06-14
CN103348017A (zh) 2013-10-09
US20170226568A1 (en) 2017-08-10
JP2014500729A (ja) 2014-01-16
SG191725A1 (en) 2013-08-30
JP6096672B2 (ja) 2017-03-15
US10533212B2 (en) 2020-01-14
US20130344485A1 (en) 2013-12-26
US9581549B2 (en) 2017-02-28
EP2649202A4 (en) 2015-05-27
CN105603071A (zh) 2016-05-25
CN103348017B (zh) 2016-01-20
AU2011338502B2 (en) 2016-08-11
AU2011338502A1 (en) 2013-06-13
EP2649202A1 (en) 2013-10-16
CN105603071B (zh) 2019-06-21
WO2012078710A1 (en) 2012-06-14

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