CA2820094C - Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides - Google Patents

Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides Download PDF

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Publication number
CA2820094C
CA2820094C CA2820094A CA2820094A CA2820094C CA 2820094 C CA2820094 C CA 2820094C CA 2820094 A CA2820094 A CA 2820094A CA 2820094 A CA2820094 A CA 2820094A CA 2820094 C CA2820094 C CA 2820094C
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nucleic acid
fluorophore
inhibitor
target nucleic
oligonucleotide
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CA2820094A
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English (en)
French (fr)
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CA2820094A1 (en
Inventor
Tal Raz
Pascaline Mary
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Dispersion Chemistry (AREA)
CA2820094A 2010-12-07 2011-12-07 Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides Active CA2820094C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US42074710P 2010-12-07 2010-12-07
US61/420,747 2010-12-07
PCT/US2011/063654 WO2012078710A1 (en) 2010-12-07 2011-12-07 Nucleic acid target detection using a detector, a probe and an inhibitor

Publications (2)

Publication Number Publication Date
CA2820094A1 CA2820094A1 (en) 2012-06-14
CA2820094C true CA2820094C (en) 2019-02-26

Family

ID=46207491

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2820094A Active CA2820094C (en) 2010-12-07 2011-12-07 Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides

Country Status (8)

Country Link
US (2) US9581549B2 (enExample)
EP (1) EP2649202B1 (enExample)
JP (1) JP6096672B2 (enExample)
CN (2) CN103348017B (enExample)
AU (1) AU2011338502B2 (enExample)
CA (1) CA2820094C (enExample)
SG (1) SG191725A1 (enExample)
WO (1) WO2012078710A1 (enExample)

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JP6096672B2 (ja) 2010-12-07 2017-03-15 ヌビオ，インコーポレイテッド 検出体、プローブ、および阻害剤を用いた核酸標的検出
SG193436A1 (en) 2011-03-30 2013-10-30 Gnubio Inc Injection of multiple volumes into or out of droplets
EP3056573B1 (en) 2011-03-31 2018-09-26 Bio-Rad Laboratories, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
JP5986623B2 (ja) 2011-03-31 2016-09-06 ヌビオ，インコーポレイテッド スケーラブルなスペクトル検出および計測
CN104781386B (zh) 2012-09-12 2018-04-06 基纽拜奥股份有限公司 用于进行试验的集成微流体系统、方法和试剂盒
WO2014117088A1 (en) 2013-01-25 2014-07-31 Gnubio, Inc. System and method for performing droplet inflation
US20160076082A1 (en) 2013-04-26 2016-03-17 Gnubio, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
EP3004813A4 (en) 2013-05-29 2016-12-21 Gnubio Inc COST-EFFECTIVE OPTICAL DISCRETE HIGH-SPEED MEASURING SYSTEM
CN105431553B (zh) * 2013-05-29 2020-02-07 生物辐射实验室股份有限公司 用于在基于乳液的微流体中测序的系统和方法
CN105765055A (zh) 2013-08-27 2016-07-13 基纽拜奥股份有限公司 微流体装置和其使用方法
WO2015048798A1 (en) 2013-09-30 2015-04-02 Gnubio, Inc. Microfluidic cartridge device and methods of use and assembly
WO2015081102A1 (en) 2013-11-27 2015-06-04 Gnubio, Inc. Microfluidic droplet packing
EP3160649B1 (en) 2014-06-30 2019-12-11 Bio-Rad Laboratories, Inc. Floating thermal contact enabled pcr
US11118217B2 (en) 2017-01-30 2021-09-14 Bio-Rad Laboratories, Inc. Emulsion compositions and methods of their use
WO2018175399A1 (en) 2017-03-24 2018-09-27 Bio-Rad Laboratories, Inc. Universal hairpin primers

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US6007994A (en) * 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization
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CN1842368B (zh) 2003-08-27 2014-05-28 哈佛大学 流体物种的电子控制
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JP5738597B2 (ja) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
CN105344389B (zh) 2008-05-16 2018-01-02 哈佛大学 微流体系统、方法和装置
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EP3004813A4 (en) 2013-05-29 2016-12-21 Gnubio Inc COST-EFFECTIVE OPTICAL DISCRETE HIGH-SPEED MEASURING SYSTEM
CN105431553B (zh) 2013-05-29 2020-02-07 生物辐射实验室股份有限公司 用于在基于乳液的微流体中测序的系统和方法
CN105765055A (zh) 2013-08-27 2016-07-13 基纽拜奥股份有限公司 微流体装置和其使用方法
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Also Published As

Publication number Publication date
WO2012078710A1 (en) 2012-06-14
AU2011338502B2 (en) 2016-08-11
US20170226568A1 (en) 2017-08-10
JP6096672B2 (ja) 2017-03-15
CN105603071A (zh) 2016-05-25
CN105603071B (zh) 2019-06-21
JP2014500729A (ja) 2014-01-16
US10533212B2 (en) 2020-01-14
CA2820094A1 (en) 2012-06-14
EP2649202B1 (en) 2019-02-20
CN103348017B (zh) 2016-01-20
US9581549B2 (en) 2017-02-28
SG191725A1 (en) 2013-08-30
US20130344485A1 (en) 2013-12-26
EP2649202A4 (en) 2015-05-27
AU2011338502A1 (en) 2013-06-13
CN103348017A (zh) 2013-10-09
EP2649202A1 (en) 2013-10-16

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