AU2011338502B2 - Nucleic acid target detection using a detector, a probe and an inhibitor - Google Patents

Nucleic acid target detection using a detector, a probe and an inhibitor Download PDF

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Publication number
AU2011338502B2
AU2011338502B2 AU2011338502A AU2011338502A AU2011338502B2 AU 2011338502 B2 AU2011338502 B2 AU 2011338502B2 AU 2011338502 A AU2011338502 A AU 2011338502A AU 2011338502 A AU2011338502 A AU 2011338502A AU 2011338502 B2 AU2011338502 B2 AU 2011338502B2
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AU
Australia
Prior art keywords
nucleic acid
inhibitor
fluorophore
target nucleic
detector
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Ceased
Application number
AU2011338502A
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English (en)
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AU2011338502A1 (en
Inventor
Mary Pascaline
Tal Raz
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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Assigned to BIO-RAD LABORATORIES, INC. reassignment BIO-RAD LABORATORIES, INC. Request for Assignment Assignors: GNUBIO, INC.
Ceased legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Dispersion Chemistry (AREA)
AU2011338502A 2010-12-07 2011-12-07 Nucleic acid target detection using a detector, a probe and an inhibitor Ceased AU2011338502B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US42074710P 2010-12-07 2010-12-07
US61/420,747 2010-12-07
PCT/US2011/063654 WO2012078710A1 (en) 2010-12-07 2011-12-07 Nucleic acid target detection using a detector, a probe and an inhibitor

Publications (2)

Publication Number Publication Date
AU2011338502A1 AU2011338502A1 (en) 2013-06-13
AU2011338502B2 true AU2011338502B2 (en) 2016-08-11

Family

ID=46207491

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2011338502A Ceased AU2011338502B2 (en) 2010-12-07 2011-12-07 Nucleic acid target detection using a detector, a probe and an inhibitor

Country Status (8)

Country Link
US (2) US9581549B2 (enExample)
EP (1) EP2649202B1 (enExample)
JP (1) JP6096672B2 (enExample)
CN (2) CN103348017B (enExample)
AU (1) AU2011338502B2 (enExample)
CA (1) CA2820094C (enExample)
SG (1) SG191725A1 (enExample)
WO (1) WO2012078710A1 (enExample)

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WO2012078710A1 (en) 2010-12-07 2012-06-14 Gnubio, Inc. Nucleic acid target detection using a detector, a probe and an inhibitor
EP2691676B1 (en) 2011-03-30 2019-03-27 Bio-Rad Laboratories, Inc. Injection of multiple volumes into or out of droplets
US9228898B2 (en) 2011-03-31 2016-01-05 Gnubio, Inc. Scalable spectroscopic detection and measurement
EP3056573B1 (en) 2011-03-31 2018-09-26 Bio-Rad Laboratories, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
US9821312B2 (en) 2012-09-12 2017-11-21 Bio-Rad Laboratories, Inc. Integrated microfluidic system, method and kit for performing assays
EP2948703B1 (en) 2013-01-25 2019-03-13 Bio-Rad Laboratories, Inc. System and method for performing droplet inflation
WO2014176599A1 (en) 2013-04-26 2014-10-30 Gnubio, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
US9809851B2 (en) 2013-05-29 2017-11-07 Bio-Rad Laboratories, Inc. Systems and methods for sequencing in emulsion based microfluidics
US9766261B2 (en) 2013-05-29 2017-09-19 Bio-Rad Laboratories, Inc. Low cost optical high speed discrete measurement system
EP3039119A4 (en) 2013-08-27 2017-04-05 GnuBIO, Inc. Microfluidic devices and methods of their use
US9555411B2 (en) 2013-09-30 2017-01-31 Gnubio, Inc. Microfluidic cartridge devices and methods of use and assembly
CN106061598B (zh) 2013-11-27 2020-08-28 生物辐射实验室股份有限公司 微流体液滴包裹
WO2016004022A2 (en) 2014-06-30 2016-01-07 Gnubio, Inc. Floating thermal contact enabled pcr
CN110234696B (zh) 2017-01-30 2022-05-31 生物辐射实验室股份有限公司 乳液组合物及其使用方法
CN110446787A (zh) 2017-03-24 2019-11-12 生物辐射实验室股份有限公司 通用发夹引物

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WO2008021446A2 (en) * 2006-08-15 2008-02-21 Genetag Technology, Inc. Probe-antiprobe compositions and methods for dna or rna detection
US20100129792A1 (en) * 2007-02-06 2010-05-27 Gerassimos Makrigiorgos Direct monitoring and pcr amplification of the dosage and dosage difference between target genetic regions

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JP5687414B2 (ja) * 2009-04-20 2015-03-18 オリンパス株式会社 多型の識別方法
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Also Published As

Publication number Publication date
SG191725A1 (en) 2013-08-30
US10533212B2 (en) 2020-01-14
CN105603071A (zh) 2016-05-25
JP6096672B2 (ja) 2017-03-15
CN105603071B (zh) 2019-06-21
CA2820094C (en) 2019-02-26
EP2649202A1 (en) 2013-10-16
EP2649202A4 (en) 2015-05-27
WO2012078710A1 (en) 2012-06-14
CA2820094A1 (en) 2012-06-14
CN103348017A (zh) 2013-10-09
JP2014500729A (ja) 2014-01-16
EP2649202B1 (en) 2019-02-20
US20130344485A1 (en) 2013-12-26
CN103348017B (zh) 2016-01-20
US9581549B2 (en) 2017-02-28
US20170226568A1 (en) 2017-08-10
AU2011338502A1 (en) 2013-06-13

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FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: BIO-RAD LABORATORIES, INC.

Free format text: FORMER OWNER(S): GNUBIO, INC.

MK14 Patent ceased section 143(a) (annual fees not paid) or expired