JP2012529904A5 - - Google Patents
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- JP2012529904A5 JP2012529904A5 JP2012515553A JP2012515553A JP2012529904A5 JP 2012529904 A5 JP2012529904 A5 JP 2012529904A5 JP 2012515553 A JP2012515553 A JP 2012515553A JP 2012515553 A JP2012515553 A JP 2012515553A JP 2012529904 A5 JP2012529904 A5 JP 2012529904A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
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- stem
- substantially complementary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims 28
- 230000000295 complement effect Effects 0.000 claims 10
- 108091033319 polynucleotide Proteins 0.000 claims 8
- 102000040430 polynucleotide Human genes 0.000 claims 8
- 108091093088 Amplicon Proteins 0.000 claims 7
- 230000003321 amplification Effects 0.000 claims 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims 6
- 230000015572 biosynthetic process Effects 0.000 claims 5
- 238000003786 synthesis reaction Methods 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 238000001514 detection method Methods 0.000 claims 3
- 244000005700 microbiome Species 0.000 claims 3
- 239000000523 sample Substances 0.000 claims 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 238000007397 LAMP assay Methods 0.000 claims 2
- 230000029918 bioluminescence Effects 0.000 claims 2
- 238000005415 bioluminescence Methods 0.000 claims 2
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims 2
- 235000011180 diphosphates Nutrition 0.000 claims 2
- 201000010099 disease Diseases 0.000 claims 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 2
- 235000003869 genetically modified organism Nutrition 0.000 claims 2
- 238000005259 measurement Methods 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 206010067484 Adverse reaction Diseases 0.000 claims 1
- 241000894006 Bacteria Species 0.000 claims 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 claims 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 claims 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims 1
- 229930010555 Inosine Natural products 0.000 claims 1
- 101000743006 Lactococcus lactis subsp. cremoris UPF0177 protein in abiGi 5'region Proteins 0.000 claims 1
- 108060001084 Luciferase Proteins 0.000 claims 1
- 239000005089 Luciferase Substances 0.000 claims 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 claims 1
- 241001465754 Metazoa Species 0.000 claims 1
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 claims 1
- 241000204031 Mycoplasma Species 0.000 claims 1
- PJKKQFAEFWCNAQ-UHFFFAOYSA-N N(4)-methylcytosine Chemical compound CNC=1C=CNC(=O)N=1 PJKKQFAEFWCNAQ-UHFFFAOYSA-N 0.000 claims 1
- 108091028664 Ribonucleotide Proteins 0.000 claims 1
- 241000191967 Staphylococcus aureus Species 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 230000006838 adverse reaction Effects 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 238000002848 electrochemical method Methods 0.000 claims 1
- 238000001962 electrophoresis Methods 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 238000003500 gene array Methods 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 claims 1
- 229960003786 inosine Drugs 0.000 claims 1
- 238000004949 mass spectrometry Methods 0.000 claims 1
- 101150008979 mecA gene Proteins 0.000 claims 1
- 229960003085 meticillin Drugs 0.000 claims 1
- 238000001668 nucleic acid synthesis Methods 0.000 claims 1
- 102000054765 polymorphisms of proteins Human genes 0.000 claims 1
- 239000002336 ribonucleotide Substances 0.000 claims 1
- 125000002652 ribonucleotide group Chemical group 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0910302.9 | 2009-06-15 | ||
| GBGB0910302.9A GB0910302D0 (en) | 2009-06-15 | 2009-06-15 | Nucleic acid amplification |
| PCT/GB2010/001169 WO2010146349A1 (en) | 2009-06-15 | 2010-06-15 | Stem accelerated isothermal nucleic acid amplification technology |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015201347A Division JP6106732B2 (ja) | 2009-06-15 | 2015-10-09 | ステム加速等温核酸増幅技術 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012529904A JP2012529904A (ja) | 2012-11-29 |
| JP2012529904A5 true JP2012529904A5 (enExample) | 2013-08-01 |
| JP5907864B2 JP5907864B2 (ja) | 2016-04-26 |
Family
ID=40940851
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012515553A Active JP5907864B2 (ja) | 2009-06-15 | 2010-06-15 | ステム加速等温核酸増幅技術 |
| JP2015201347A Expired - Fee Related JP6106732B2 (ja) | 2009-06-15 | 2015-10-09 | ステム加速等温核酸増幅技術 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015201347A Expired - Fee Related JP6106732B2 (ja) | 2009-06-15 | 2015-10-09 | ステム加速等温核酸増幅技術 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9410190B2 (enExample) |
| EP (1) | EP2443253B1 (enExample) |
| JP (2) | JP5907864B2 (enExample) |
| CN (1) | CN102652176B (enExample) |
| AU (1) | AU2010261541B2 (enExample) |
| CA (1) | CA2765406C (enExample) |
| ES (1) | ES2589079T3 (enExample) |
| GB (1) | GB0910302D0 (enExample) |
| WO (1) | WO2010146349A1 (enExample) |
| ZA (1) | ZA201109493B (enExample) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2010209528B2 (en) | 2009-01-30 | 2015-10-01 | Oxford Nanopore Technologies Limited | Adaptors for nucleic acid constructs in transmembrane sequencing |
| US9284602B2 (en) | 2010-10-27 | 2016-03-15 | President And Fellows Of Harvard College | Compositions of toehold primer duplexes and methods of use |
| CA2835283C (en) | 2011-05-24 | 2019-08-06 | Elitech Holding B.V. | Detection of methicillin-resistant staphylococcus aureus |
| JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| EP2875154B1 (en) | 2012-07-19 | 2017-08-23 | Oxford Nanopore Technologies Limited | SSB method for characterising a nucleic acid |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| CA2906824C (en) | 2013-03-15 | 2023-10-03 | Theranos, Inc. | Nucleic acid amplification |
| US10450595B2 (en) | 2013-03-15 | 2019-10-22 | Theranos Ip Company, Llc | Nucleic acid amplification |
| KR20150132481A (ko) * | 2013-03-15 | 2015-11-25 | 테라노스, 인코포레이티드 | 핵산 증폭 |
| US9677981B2 (en) | 2013-03-15 | 2017-06-13 | 3M Innovative Properties Company | Sample concentrator and method of use |
| CN105452480B (zh) * | 2013-08-09 | 2020-01-31 | 基因生物系统股份有限公司 | 经由剪刀状结构的dna扩增(dasl) |
| US9529976B2 (en) | 2013-09-06 | 2016-12-27 | Theranos, Inc. | Systems and methods for detecting infectious diseases |
| CN103497947B (zh) * | 2013-09-13 | 2015-08-12 | 浙江国际旅行卫生保健中心 | 一种体外新型快速环介导等温扩增方法、体外检测甲型h1n1流感病毒的试剂及方法 |
| EP3071713B1 (en) * | 2013-11-22 | 2021-05-12 | Labrador Diagnostics LLC | Nucleic acid amplification |
| US10767222B2 (en) | 2013-12-11 | 2020-09-08 | Accuragen Holdings Limited | Compositions and methods for detecting rare sequence variants |
| US11859246B2 (en) | 2013-12-11 | 2024-01-02 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| US11286519B2 (en) | 2013-12-11 | 2022-03-29 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| US10716587B2 (en) | 2014-06-13 | 2020-07-21 | Surgis Medical Llc | Surgical device with light |
| WO2016011280A1 (en) | 2014-07-16 | 2016-01-21 | Tangen Biosciences, Inc. | Isothermal methods for amplifying nucleic acid samples |
| EP3215260B1 (en) | 2014-11-03 | 2020-01-15 | Tangen Biosciences Inc. | Apparatus and method for cell, spore, or virus capture and disruption |
| CN107208160B (zh) | 2015-01-30 | 2023-02-17 | 哈佛学院院长及董事 | 无显微镜成像 |
| GB2550712B (en) * | 2015-02-26 | 2021-01-13 | Hitachi High Tech Corp | Method for constructing hairpin-containing nucleic acid molecules for nanopore sequencing |
| US10260090B2 (en) | 2015-07-16 | 2019-04-16 | Arizona Board Of Regents On Behalf Of Arizona State University | Accelerated isothermal amplification of DNA |
| CN114807323A (zh) * | 2015-10-09 | 2022-07-29 | 安可济控股有限公司 | 用于富集扩增产物的方法及组合物 |
| CN108699505A (zh) | 2015-12-03 | 2018-10-23 | 安可济控股有限公司 | 用于形成连接产物的方法和组合物 |
| JP7085999B2 (ja) | 2016-02-17 | 2022-06-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 分子プログラミングツール |
| CA3024630A1 (en) | 2016-05-16 | 2017-11-23 | Accuragen Holdings Limited | Method of improved sequencing by strand identification |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| CN107488656B (zh) | 2016-06-13 | 2020-07-17 | 陆欣华 | 一种核酸等温自扩增方法 |
| WO2018035170A1 (en) | 2016-08-15 | 2018-02-22 | Accuragen Holdings Limited | Compositions and methods for detecting rare sequence variants |
| JP7116062B2 (ja) | 2017-01-10 | 2022-08-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 多重化シグナル増幅 |
| CA3123182A1 (en) | 2017-12-14 | 2019-06-20 | Medicinal Genomics Corporation | Methods and kits for classifying cannabinoid production in cannabis plants |
| WO2019147945A1 (en) | 2018-01-26 | 2019-08-01 | President And Fellows Of Harvard College | Proximity detection methods and compositions |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| CN112601823A (zh) | 2018-06-12 | 2021-04-02 | 安可济控股有限公司 | 用于形成连接产物的方法和组合物 |
| CN114045330B (zh) * | 2021-12-23 | 2024-02-09 | 川北医学院附属医院 | 一种基于滑动复制的核酸等温扩增方法 |
| CN118621045A (zh) * | 2024-08-14 | 2024-09-10 | 天健生物制药（天津）有限公司 | 基于lamp的鸟分枝杆菌检测的组合物和试剂盒 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5866336A (en) * | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| WO2002024902A1 (en) * | 2000-09-19 | 2002-03-28 | Eiken Kagaku Kabushiki Kaisha | Method of synthesizing polynucleotide |
| JPWO2002090538A1 (ja) * | 2001-05-08 | 2004-08-26 | 栄研化学株式会社 | 核酸を合成する方法 |
| GB0218215D0 (en) | 2002-08-06 | 2002-09-11 | Tepnel Medical Ltd | Amplification of nucleic acids |
| ATE532878T1 (de) | 2005-08-24 | 2011-11-15 | Life Technologies Corp | Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas |
| JP4580875B2 (ja) * | 2006-01-20 | 2010-11-17 | 株式会社東芝 | 核酸検出法のためのプライマーの設計方法 |
| JP2008029333A (ja) | 2006-07-03 | 2008-02-14 | Fujifilm Corp | 新規遺伝子増幅法に用いられるプライマー |
| WO2008043987A2 (en) | 2006-10-09 | 2008-04-17 | Oxitec Limited | Methods for amplifying and detecting nucleic acid sequences |
| JP4490988B2 (ja) * | 2007-04-26 | 2010-06-30 | 株式会社東芝 | 血清アミロイドａ１（ｓａａ１）の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法 |
| US8748100B2 (en) | 2007-08-30 | 2014-06-10 | The Chinese University Of Hong Kong | Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences |
-
2009
- 2009-06-15 GB GBGB0910302.9A patent/GB0910302D0/en not_active Ceased
-
2010
- 2010-06-15 US US13/377,704 patent/US9410190B2/en active Active
- 2010-06-15 JP JP2012515553A patent/JP5907864B2/ja active Active
- 2010-06-15 CN CN201080035868.5A patent/CN102652176B/zh active Active
- 2010-06-15 ES ES10725825.3T patent/ES2589079T3/es active Active
- 2010-06-15 WO PCT/GB2010/001169 patent/WO2010146349A1/en not_active Ceased
- 2010-06-15 EP EP10725825.3A patent/EP2443253B1/en active Active
- 2010-06-15 AU AU2010261541A patent/AU2010261541B2/en active Active
- 2010-06-15 CA CA2765406A patent/CA2765406C/en active Active
-
2011
- 2011-12-22 ZA ZA2011/09493A patent/ZA201109493B/en unknown
-
2015
- 2015-10-09 JP JP2015201347A patent/JP6106732B2/ja not_active Expired - Fee Related
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