CA2765406C - Stem accelerated isothermal nucleic acid amplification technology - Google Patents

Stem accelerated isothermal nucleic acid amplification technology Download PDF

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Publication number
CA2765406C
CA2765406C CA2765406A CA2765406A CA2765406C CA 2765406 C CA2765406 C CA 2765406C CA 2765406 A CA2765406 A CA 2765406A CA 2765406 A CA2765406 A CA 2765406A CA 2765406 C CA2765406 C CA 2765406C
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Canada
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primer
primers
stem
region
segment
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CA2765406A
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English (en)
French (fr)
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CA2765406A1 (en
Inventor
Laurence Carlo Tisi
Olga Gandelmann
Guy Kiddle
Cathal Joseph Mcelgunn
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Lumora Ltd
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Lumora Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2765406A 2009-06-15 2010-06-15 Stem accelerated isothermal nucleic acid amplification technology Active CA2765406C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0910302.9A GB0910302D0 (en) 2009-06-15 2009-06-15 Nucleic acid amplification
GB0910302.9 2009-06-15
PCT/GB2010/001169 WO2010146349A1 (en) 2009-06-15 2010-06-15 Stem accelerated isothermal nucleic acid amplification technology

Publications (2)

Publication Number Publication Date
CA2765406A1 CA2765406A1 (en) 2010-12-23
CA2765406C true CA2765406C (en) 2019-02-26

Family

ID=40940851

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2765406A Active CA2765406C (en) 2009-06-15 2010-06-15 Stem accelerated isothermal nucleic acid amplification technology

Country Status (10)

Country Link
US (1) US9410190B2 (enExample)
EP (1) EP2443253B1 (enExample)
JP (2) JP5907864B2 (enExample)
CN (1) CN102652176B (enExample)
AU (1) AU2010261541B2 (enExample)
CA (1) CA2765406C (enExample)
ES (1) ES2589079T3 (enExample)
GB (1) GB0910302D0 (enExample)
WO (1) WO2010146349A1 (enExample)
ZA (1) ZA201109493B (enExample)

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EP2633071B1 (en) 2010-10-27 2016-10-12 President and Fellows of Harvard College Compositions of toehold primer duplexes and methods of use
ES2537189T3 (es) * 2011-05-24 2015-06-03 Elitech Holding B.V. Detección de estafilococos áureos resistentes a la meticilina
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
CN116334193A (zh) * 2013-03-15 2023-06-27 赛拉诺斯知识产权有限责任公司 核酸扩增
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
US9677981B2 (en) 2013-03-15 2017-06-13 3M Innovative Properties Company Sample concentrator and method of use
SG11201507273SA (en) 2013-03-15 2015-10-29 Theranos Inc Nucleic acid amplification
WO2015019247A1 (en) * 2013-08-09 2015-02-12 Genebio Systems, Inc. Dna amplification via scissor-like structures (dasl)
SG11201601611WA (en) 2013-09-06 2016-04-28 Theranos Inc Systems and methods for detecting infectious diseases
CN103497947B (zh) * 2013-09-13 2015-08-12 浙江国际旅行卫生保健中心 一种体外新型快速环介导等温扩增方法、体外检测甲型h1n1流感病毒的试剂及方法
MX370616B (es) * 2013-11-22 2019-12-17 Theranos Ip Co Llc Amplificacion de acidos nucleicos.
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US10767222B2 (en) 2013-12-11 2020-09-08 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
WO2017062863A1 (en) 2015-10-09 2017-04-13 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10716587B2 (en) 2014-06-13 2020-07-21 Surgis Medical Llc Surgical device with light
US10364458B2 (en) 2014-07-16 2019-07-30 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
EP3215260B1 (en) 2014-11-03 2020-01-15 Tangen Biosciences Inc. Apparatus and method for cell, spore, or virus capture and disruption
US11639522B2 (en) 2015-01-30 2023-05-02 President And Fellows Of Harvard College Microscope-free imaging
GB2550712B (en) * 2015-02-26 2021-01-13 Hitachi High Tech Corp Method for constructing hairpin-containing nucleic acid molecules for nanopore sequencing
US10260090B2 (en) 2015-07-16 2019-04-16 Arizona Board Of Regents On Behalf Of Arizona State University Accelerated isothermal amplification of DNA
WO2017096322A1 (en) 2015-12-03 2017-06-08 Accuragen Holdings Limited Methods and compositions for forming ligation products
EP4239079B1 (en) 2016-02-17 2025-12-17 President And Fellows Of Harvard College Molecular programming tools
CN109511265B (zh) 2016-05-16 2023-07-14 安可济控股有限公司 通过链鉴定改进测序的方法
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN107488656B (zh) * 2016-06-13 2020-07-17 陆欣华 一种核酸等温自扩增方法
CN109844133A (zh) 2016-08-15 2019-06-04 安可济控股有限公司 检测罕见序列变体的组合物和方法
JP7116062B2 (ja) * 2017-01-10 2022-08-09 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重化シグナル増幅
EP3724322A1 (en) 2017-12-14 2020-10-21 Medicinal Genomics Corporation Methods and kits for classifying cannabinoid production in cannabis plants
CN111630179B (zh) 2018-01-26 2024-07-19 哈佛学院院长及董事 邻近检测方法和组合物
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
WO2019241290A1 (en) 2018-06-12 2019-12-19 Accuragen Holdings Limited Methods and compositions for forming ligation products
US12553080B2 (en) 2021-06-14 2026-02-17 Delta Electronics Int'l (Singapore) Pte Ltd Lamp primer set and method for amplifying nucleic acids using the same
CN114045330B (zh) * 2021-12-23 2024-02-09 川北医学院附属医院 一种基于滑动复制的核酸等温扩增方法
CN118621045A (zh) * 2024-08-14 2024-09-10 天健生物制药（天津）有限公司 基于lamp的鸟分枝杆菌检测的组合物和试剂盒
WO2026050948A1 (zh) * 2024-09-04 2026-03-12 深圳华大生命科学研究院 核酸文库的构建方法及应用

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US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
ES2327712T3 (es) * 2000-09-19 2009-11-03 Eiken Kagaku Kabushiki Kaisha Procedimiento para sintetizar polinucleotidos.
WO2002090538A1 (en) 2001-05-08 2002-11-14 Eiken Kagaku Kabushiki Kaisha Method of synthesizing nucleic acid
GB0218215D0 (en) 2002-08-06 2002-09-11 Tepnel Medical Ltd Amplification of nucleic acids
EP1924713B1 (en) 2005-08-24 2011-11-09 Life Technologies Corporation A method to quantify sirnas, mirnas and polymorphic mirnas
JP4580875B2 (ja) * 2006-01-20 2010-11-17 株式会社東芝 核酸検出法のためのプライマーの設計方法
JP2008029333A (ja) 2006-07-03 2008-02-14 Fujifilm Corp 新規遺伝子増幅法に用いられるプライマー
EP2069540B1 (en) 2006-10-09 2014-03-19 Oxitec Limited Methods for amplifying and detecting nucleic acid sequences
JP4490988B2 (ja) 2007-04-26 2010-06-30 株式会社東芝 血清アミロイドａ１（ｓａａ１）の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法
US8748100B2 (en) 2007-08-30 2014-06-10 The Chinese University Of Hong Kong Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences

Also Published As

Publication number Publication date
US9410190B2 (en) 2016-08-09
US20120157326A1 (en) 2012-06-21
WO2010146349A1 (en) 2010-12-23
GB0910302D0 (en) 2009-07-29
EP2443253B1 (en) 2016-06-08
JP2012529904A (ja) 2012-11-29
CN102652176A (zh) 2012-08-29
CA2765406A1 (en) 2010-12-23
JP2016041070A (ja) 2016-03-31
JP5907864B2 (ja) 2016-04-26
ES2589079T3 (es) 2016-11-10
ZA201109493B (en) 2015-07-29
CN102652176B (zh) 2016-09-28
AU2010261541B2 (en) 2016-03-10
AU2010261541A1 (en) 2012-02-02
JP6106732B2 (ja) 2017-04-05
EP2443253A1 (en) 2012-04-25

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