JP2014210797A5 - - Google Patents
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- Publication number
- JP2014210797A5 JP2014210797A5 JP2014137837A JP2014137837A JP2014210797A5 JP 2014210797 A5 JP2014210797 A5 JP 2014210797A5 JP 2014137837 A JP2014137837 A JP 2014137837A JP 2014137837 A JP2014137837 A JP 2014137837A JP 2014210797 A5 JP2014210797 A5 JP 2014210797A5
- Authority
- JP
- Japan
- Prior art keywords
- chromatography
- steps
- chromatography step
- hydrophobic
- charge induction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000011210 chromatographic step Methods 0.000 claims 41
- 108090000765 processed proteins & peptides Proteins 0.000 claims 27
- 229920001184 polypeptide Polymers 0.000 claims 26
- 102000004196 processed proteins & peptides Human genes 0.000 claims 26
- 238000000034 method Methods 0.000 claims 19
- 238000004587 chromatography analysis Methods 0.000 claims 16
- 230000002209 hydrophobic effect Effects 0.000 claims 15
- 230000006698 induction Effects 0.000 claims 15
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 13
- 238000005277 cation exchange chromatography Methods 0.000 claims 11
- 210000001236 prokaryotic cell Anatomy 0.000 claims 10
- 238000005571 anion exchange chromatography Methods 0.000 claims 9
- 238000001042 affinity chromatography Methods 0.000 claims 7
- 238000012872 hydroxylapatite chromatography Methods 0.000 claims 6
- 238000004519 manufacturing process Methods 0.000 claims 5
- 238000000746 purification Methods 0.000 claims 5
- 210000003000 inclusion body Anatomy 0.000 claims 4
- 239000002184 metal Substances 0.000 claims 4
- 241000588724 Escherichia coli Species 0.000 claims 3
- 102000014150 Interferons Human genes 0.000 claims 3
- 108010050904 Interferons Proteins 0.000 claims 3
- 238000004255 ion exchange chromatography Methods 0.000 claims 3
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 claims 2
- 102000004218 Insulin-Like Growth Factor I Human genes 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 229940079322 interferon Drugs 0.000 claims 2
- 239000003446 ligand Substances 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 239000000556 agonist Substances 0.000 claims 1
- 239000005557 antagonist Substances 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000013522 chelant Substances 0.000 claims 1
- 239000002158 endotoxin Substances 0.000 claims 1
- 239000003102 growth factor Substances 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 229940047124 interferons Drugs 0.000 claims 1
- 230000006320 pegylation Effects 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000001172 regenerating effect Effects 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08000884 | 2008-01-18 | ||
| EP08000884.0 | 2008-01-18 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010542579A Division JP5944101B2 (ja) | 2008-01-18 | 2009-01-15 | 非グリコシル化タンパク質の精製 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2014210797A JP2014210797A (ja) | 2014-11-13 |
| JP2014210797A5 true JP2014210797A5 (https=) | 2015-05-28 |
Family
ID=39810157
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010542579A Expired - Fee Related JP5944101B2 (ja) | 2008-01-18 | 2009-01-15 | 非グリコシル化タンパク質の精製 |
| JP2014137837A Pending JP2014210797A (ja) | 2008-01-18 | 2014-07-03 | 非グリコシル化タンパク質の精製 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010542579A Expired - Fee Related JP5944101B2 (ja) | 2008-01-18 | 2009-01-15 | 非グリコシル化タンパク質の精製 |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US20110034672A1 (https=) |
| EP (1) | EP2245044A2 (https=) |
| JP (2) | JP5944101B2 (https=) |
| KR (1) | KR20100103595A (https=) |
| CN (2) | CN107033233A (https=) |
| AU (1) | AU2009204998B2 (https=) |
| CA (1) | CA2711375C (https=) |
| IL (1) | IL206158A (https=) |
| WO (1) | WO2009090056A2 (https=) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009090056A2 (en) * | 2008-01-18 | 2009-07-23 | F. Hoffmann-La Roche Ag | Purification of not-glycosylated polypeptides |
| DE102009032179A1 (de) * | 2009-07-07 | 2011-01-13 | Biogenerix Ag | Verfahren zur Reinigung von Interferon beta |
| CN102219848B (zh) * | 2011-05-25 | 2014-01-22 | 浙江海正药业股份有限公司 | 重组人干扰素β-1a的纯化方法 |
| US20140154233A1 (en) | 2012-12-05 | 2014-06-05 | Csl Limited | Method of purifying therapeutic proteins |
| US10188965B2 (en) | 2012-12-05 | 2019-01-29 | Csl Behring Gmbh | Hydrophobic charge induction chromatographic depletion of a protein from a solution |
| CN104710527B (zh) * | 2015-02-28 | 2018-08-24 | 苏州金盟生物技术有限公司 | 一种生物制品的内毒素去除方法 |
| KR102775456B1 (ko) * | 2015-11-09 | 2025-02-28 | 바이오로지칼 이 리미티드 | 생물학적 활성 재조합 캐리어 단백질을 회수하기 위한 산업적 확장성이 있는 방법 |
| TW202128264A (zh) * | 2019-09-25 | 2021-08-01 | 瑞士商赫孚孟拉羅股份公司 | 使用經預測之溶離緩衝鹽濃度的陽離子層析法 |
| US20220227805A1 (en) * | 2020-12-23 | 2022-07-21 | Jazz Pharmaceuticals Ireland Ltd. | Methods of purifying charge-shielded fusion proteins |
| CN113480632B (zh) * | 2021-07-30 | 2023-02-03 | 宁波人健药业集团股份有限公司 | 一种在CHO细胞中表达的重组蛋白rhCG纯化工艺 |
| TW202403043A (zh) | 2022-03-18 | 2024-01-16 | 美商健臻公司 | 重組人類酸性鞘磷脂酶醫藥組合物及方法 |
| CN117024561B (zh) * | 2023-10-10 | 2024-02-20 | 哈药集团生物工程有限公司 | 一种聚乙二醇修饰干扰素的纯化方法 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH657141A5 (de) * | 1980-07-01 | 1986-08-15 | Hoffmann La Roche | Dns-sequenzen, rekombinante expressionsvektoren zur mikrobiellen herstellung von human-leukozyten-interferonen und transformierte mikroorganismen. |
| US5696238A (en) * | 1991-08-20 | 1997-12-09 | Chiron Corporation | Purified GP120 composition retaining natural conformation |
| EP0626448A3 (de) * | 1993-05-26 | 1998-01-14 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Verfahren zur Herstellung und Reinigung von alpha-Interferon |
| DE4329756A1 (de) * | 1993-09-03 | 1995-03-09 | Boehringer Ingelheim Int | Verfahren zur Herstellung und Reinigung von alpha-Interferon |
| DK0679718T3 (da) | 1994-04-09 | 2001-10-22 | Hoffmann La Roche | Fremgangsmåde til fremstilling af alfa-interferon |
| TW517067B (en) * | 1996-05-31 | 2003-01-11 | Hoffmann La Roche | Interferon conjugates |
| KR100443890B1 (ko) | 2001-04-11 | 2004-08-09 | 한미약품 주식회사 | 재조합 대장균으로부터 인간 성장 호르몬의 정제 방법 |
| CN1233816C (zh) * | 2002-04-16 | 2005-12-28 | 海南海梁生物高科技有限公司 | 酵母重组株及IFNα－la干扰素的纯化方法 |
| CA2478925C (en) * | 2002-04-26 | 2016-06-07 | Robert Lee Fahrner | Non-affinity purification of proteins |
| ES2391457T3 (es) * | 2003-08-13 | 2012-11-26 | Sandoz Ag | Procedimiento para la purificación de polipéptidos recombinantes |
| RS50516B (sr) | 2003-11-05 | 2010-05-07 | Ares Trading S.A. | Prečišćavanje il-18 vezujućeg proteina |
| CA2544333A1 (en) | 2003-12-22 | 2005-07-14 | Ares Trading S.A. | Method for purifying fsh |
| SE0400886D0 (sv) * | 2004-04-02 | 2004-04-02 | Amersham Biosciences Ab | Process of purification |
| AU2005245918A1 (en) * | 2004-05-19 | 2005-12-01 | F. Hoffmann-La Roche Ag | Interferon-alpha polypeptides and conjugates |
| WO2006101441A2 (en) | 2005-03-24 | 2006-09-28 | Straumann Holding Ag | Method for protein purification comprising heat incubation in acetic acidic solution |
| WO2007016250A1 (en) | 2005-07-28 | 2007-02-08 | Genvec, Inc. | Method of purifying protein |
| CN1916024A (zh) * | 2005-08-17 | 2007-02-21 | 海南国栋药物研究所有限公司 | 高比活人α－2b干扰素突变体序列、酵母表达质粒的构建及菌株筛选和纯化方法 |
| WO2007041713A1 (en) * | 2005-10-04 | 2007-04-12 | Zymogenetics, Inc. | Production and purification of il-29 |
| WO2007075283A2 (en) | 2005-12-06 | 2007-07-05 | Amgen Inc. | Polishing steps used in multi-step protein purification processes |
| WO2009090056A2 (en) * | 2008-01-18 | 2009-07-23 | F. Hoffmann-La Roche Ag | Purification of not-glycosylated polypeptides |
| CN103864915B (zh) * | 2012-12-07 | 2016-06-08 | 天津未名生物医药有限公司 | 纯化重组人干扰素α2b的方法和试剂盒 |
| CN104356223B (zh) * | 2014-11-26 | 2017-11-10 | 安徽安科生物工程（集团）股份有限公司 | 高纯度重组人干扰素α2b的制备方法 |
-
2009
- 2009-01-15 WO PCT/EP2009/000192 patent/WO2009090056A2/en not_active Ceased
- 2009-01-15 JP JP2010542579A patent/JP5944101B2/ja not_active Expired - Fee Related
- 2009-01-15 US US12/811,397 patent/US20110034672A1/en not_active Abandoned
- 2009-01-15 EP EP09702937A patent/EP2245044A2/en not_active Withdrawn
- 2009-01-15 CN CN201710044891.4A patent/CN107033233A/zh active Pending
- 2009-01-15 CA CA2711375A patent/CA2711375C/en not_active Expired - Fee Related
- 2009-01-15 CN CN2009801023313A patent/CN101918430A/zh active Pending
- 2009-01-15 AU AU2009204998A patent/AU2009204998B2/en not_active Ceased
- 2009-01-15 KR KR1020107015694A patent/KR20100103595A/ko not_active Ceased
-
2010
- 2010-06-03 IL IL206158A patent/IL206158A/en active IP Right Grant
-
2012
- 2012-09-21 US US13/624,091 patent/US20130190478A1/en not_active Abandoned
-
2014
- 2014-04-07 US US14/246,783 patent/US9481706B2/en not_active Expired - Fee Related
- 2014-07-03 JP JP2014137837A patent/JP2014210797A/ja active Pending
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